Dynamic motion of paxillin on actin filaments in living endothelial cells
Our three-dimensional (3-D) images showed that paxillin co-localized on actin filaments as fibrous structures, as well as clusters, in endothelial cells (ECs). In living ECs under flow condition, we monitored concurrently the intracellular dynamics of DsRed2-paxillin and GFP-actin by time-lapse vide...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2007-06, Vol.357 (4), p.871-876 |
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| creator | Hu, Ying-Li Chien, Shu |
| description | Our three-dimensional (3-D) images showed that paxillin co-localized on actin filaments as fibrous structures, as well as clusters, in endothelial cells (ECs). In living ECs under flow condition, we monitored concurrently the intracellular dynamics of DsRed2-paxillin and GFP-actin by time-lapse video recording and dual-color fluorescence imaging. The results showed that the dynamic motion of paxillin as fibrous structures was associated with actin filaments, but not with microtubules. Our findings suggest that the actin network plays an important role not only in the assembly/disassembly of paxillin at focal adhesions, but also as a track for the intracellular transport of paxillin, which is involved in signaling pathway. |
| doi_str_mv | 10.1016/j.bbrc.2007.04.028 |
| format | Article |
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Our findings suggest that the actin network plays an important role not only in the assembly/disassembly of paxillin at focal adhesions, but also as a track for the intracellular transport of paxillin, which is involved in signaling pathway.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2007.04.028</identifier><identifier>PMID: 17466945</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Actin Cytoskeleton - physiology ; Actin Cytoskeleton - ultrastructure ; Actin filaments ; Animals ; Cattle ; Cell Movement - physiology ; Cells, Cultured ; Endothelial Cells - physiology ; Endothelial Cells - ultrastructure ; Focal adhesions ; Microtubules ; Molecular Motor Proteins - physiology ; Molecular Motor Proteins - ultrastructure ; Movement - physiology ; Paxillin ; Paxillin - physiology ; Paxillin - ultrastructure ; Protein Binding</subject><ispartof>Biochemical and biophysical research communications, 2007-06, Vol.357 (4), p.871-876</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c519t-d5414a9bf8667c028aaba53515411ed6594e7376717293d6903376fcc975cb453</citedby><cites>FETCH-LOGICAL-c519t-d5414a9bf8667c028aaba53515411ed6594e7376717293d6903376fcc975cb453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X07006973$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17466945$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Ying-Li</creatorcontrib><creatorcontrib>Chien, Shu</creatorcontrib><title>Dynamic motion of paxillin on actin filaments in living endothelial cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Our three-dimensional (3-D) images showed that paxillin co-localized on actin filaments as fibrous structures, as well as clusters, in endothelial cells (ECs). 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| ispartof | Biochemical and biophysical research communications, 2007-06, Vol.357 (4), p.871-876 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Actin Cytoskeleton - physiology Actin Cytoskeleton - ultrastructure Actin filaments Animals Cattle Cell Movement - physiology Cells, Cultured Endothelial Cells - physiology Endothelial Cells - ultrastructure Focal adhesions Microtubules Molecular Motor Proteins - physiology Molecular Motor Proteins - ultrastructure Movement - physiology Paxillin Paxillin - physiology Paxillin - ultrastructure Protein Binding |
| title | Dynamic motion of paxillin on actin filaments in living endothelial cells |
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