Agrobacterium Transcriptional Regulator Ros is a Prokaryotic Zinc Finger Protein that Regulates the Plant Oncogene ipt
Virulence genes of Agrobacterium tumefaciens are under the control of positive and negative transcriptional regulators. We found that the transcriptional regulator Ros controls expression of the plant oncogene ipt, which encodes isopentenyl transferase, in A. tumefaciens. This enzyme is involved in...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1998-04, Vol.95 (9), p.5293-5298 |
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| creator | Chou, Alan Y. Archdeacon, John Kado, Clarence I. |
| description | Virulence genes of Agrobacterium tumefaciens are under the control of positive and negative transcriptional regulators. We found that the transcriptional regulator Ros controls expression of the plant oncogene ipt, which encodes isopentenyl transferase, in A. tumefaciens. This enzyme is involved in biosynthesis of the plant growth hormone cytokinin in the host plant. An ipt promoter::cat reporter gene fusion showed a 10-fold increase in ipt promoter activity in A. tumefaciens ros mutant strains when compared with wild type. Also, increased levels (10-to 20-fold) of isopentenyl adenosine, the product of the reaction catalyzed by isopentenyl transferase, were detected in ros mutant strains. In vitro studies using purified Ros showed it binds directly to the ipt promoter. Analysis of the deduced Ros amino acid sequence identified a novel type of C2H2zinc finger. In Ros the peptide loop spacing of the zinc finger is 9 amino acids as opposed to the invariant 12 amino acids in the classical C2H2motif. Site-directed mutagenesis of Cys-82 and His-92 in this motif showed that these residues are essential for Zn2+and DNA binding activities of Ros. The existence of such a regulator in Agrobacterium may be due to horizontal interkingdom retrotransfer of the ros gene from plant to bacteria. |
| doi_str_mv | 10.1073/pnas.95.9.5293 |
| format | Article |
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We found that the transcriptional regulator Ros controls expression of the plant oncogene ipt, which encodes isopentenyl transferase, in A. tumefaciens. This enzyme is involved in biosynthesis of the plant growth hormone cytokinin in the host plant. An ipt promoter::cat reporter gene fusion showed a 10-fold increase in ipt promoter activity in A. tumefaciens ros mutant strains when compared with wild type. Also, increased levels (10-to 20-fold) of isopentenyl adenosine, the product of the reaction catalyzed by isopentenyl transferase, were detected in ros mutant strains. In vitro studies using purified Ros showed it binds directly to the ipt promoter. Analysis of the deduced Ros amino acid sequence identified a novel type of C2H2zinc finger. In Ros the peptide loop spacing of the zinc finger is 9 amino acids as opposed to the invariant 12 amino acids in the classical C2H2motif. Site-directed mutagenesis of Cys-82 and His-92 in this motif showed that these residues are essential for Zn2+and DNA binding activities of Ros. The existence of such a regulator in Agrobacterium may be due to horizontal interkingdom retrotransfer of the ros gene from plant to bacteria.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.95.9.5293</identifier><identifier>PMID: 9560269</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Agrobacterium ; Agrobacterium tumefaciens - enzymology ; Alkyl and Aryl Transferases - genetics ; Amino Acid Sequence ; Amino acids ; Bacterial Proteins ; Binding sites ; Biological Sciences ; Cytokinins ; DNA ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - physiology ; Flowers & plants ; Gels ; Gene Expression Regulation, Bacterial ; Gene Expression Regulation, Plant ; Genes ; Isopentenyladenosine - metabolism ; Microbiology ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Oncogenes ; Plant growth ; Promoter Regions, Genetic ; Proteins ; Repressor Proteins - physiology ; Sequence Alignment ; Sequence Homology, Amino Acid ; Transcription, Genetic ; Transcriptional regulatory elements ; Transferases - genetics ; Zinc ; Zinc Fingers</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1998-04, Vol.95 (9), p.5293-5298</ispartof><rights>Copyright 1993-1998 National Academy of Sciences</rights><rights>Copyright National Academy of Sciences Apr 28, 1998</rights><rights>Copyright © 1998, The National Academy of Sciences 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-d20dc15f3d9e312da38a6ca9a33301cc4b18b1edf0dc0f2fe69c2052d455d9203</citedby><cites>FETCH-LOGICAL-c513t-d20dc15f3d9e312da38a6ca9a33301cc4b18b1edf0dc0f2fe69c2052d455d9203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/95/9.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/44707$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/44707$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53769,53771,57995,58228</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9560269$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chou, Alan Y.</creatorcontrib><creatorcontrib>Archdeacon, John</creatorcontrib><creatorcontrib>Kado, Clarence I.</creatorcontrib><title>Agrobacterium Transcriptional Regulator Ros is a Prokaryotic Zinc Finger Protein that Regulates the Plant Oncogene ipt</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Virulence genes of Agrobacterium tumefaciens are under the control of positive and negative transcriptional regulators. We found that the transcriptional regulator Ros controls expression of the plant oncogene ipt, which encodes isopentenyl transferase, in A. tumefaciens. This enzyme is involved in biosynthesis of the plant growth hormone cytokinin in the host plant. An ipt promoter::cat reporter gene fusion showed a 10-fold increase in ipt promoter activity in A. tumefaciens ros mutant strains when compared with wild type. Also, increased levels (10-to 20-fold) of isopentenyl adenosine, the product of the reaction catalyzed by isopentenyl transferase, were detected in ros mutant strains. In vitro studies using purified Ros showed it binds directly to the ipt promoter. Analysis of the deduced Ros amino acid sequence identified a novel type of C2H2zinc finger. In Ros the peptide loop spacing of the zinc finger is 9 amino acids as opposed to the invariant 12 amino acids in the classical C2H2motif. Site-directed mutagenesis of Cys-82 and His-92 in this motif showed that these residues are essential for Zn2+and DNA binding activities of Ros. The existence of such a regulator in Agrobacterium may be due to horizontal interkingdom retrotransfer of the ros gene from plant to bacteria.</description><subject>Agrobacterium</subject><subject>Agrobacterium tumefaciens - enzymology</subject><subject>Alkyl and Aryl Transferases - genetics</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Bacterial Proteins</subject><subject>Binding sites</subject><subject>Biological Sciences</subject><subject>Cytokinins</subject><subject>DNA</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Flowers & plants</subject><subject>Gels</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Isopentenyladenosine - metabolism</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Site-Directed</subject><subject>Oncogenes</subject><subject>Plant growth</subject><subject>Promoter Regions, Genetic</subject><subject>Proteins</subject><subject>Repressor Proteins - physiology</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Transcription, Genetic</subject><subject>Transcriptional regulatory elements</subject><subject>Transferases - genetics</subject><subject>Zinc</subject><subject>Zinc Fingers</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc9rFDEcxYModa1ePQhC8NDbjPk5swEvpVgVCi2lXryEbOY706yzyZpkiv73zbDruoqnkLzP-_K-eQi9pqSmpOXvt96kWsla1ZIp_gQtKFG0aoQiT9GCENZWS8HEc_QipTUhRMklOUEnSjaENWqBHs6HGFbGZohu2uC7aHyy0W2zC96M-BaGaTQ5RHwbEnYJG3wTw3cTf4XsLP7mvMWXzg8Q5_cMzuN8b_JvH6RyBXwzGp_xtbdhAA-4TH-JnvVmTPBqf56ir5cf7y4-V1fXn75cnF9VVlKeq46RzlLZ804Bp6wzfGkaa5ThnBNqrVjR5YpC1xeM9KyHRllGJOuElJ1ihJ-iD7u522m1gc6Cz9GMehvdpuygg3H6b8W7ez2EB80Ik6LYz_b2GH5MkLLeuGRhLPtAmJKmDW-ppLKA7_4B12GK5QdTmUR504pGFajeQTaGlCL0hxyU6LlMPZepldRKz2UWw9vj9Ad8395RvNn3R937dT-NY4af-WjQf8Giv9np61S6PgBCtCXVI9ujvpk</recordid><startdate>19980428</startdate><enddate>19980428</enddate><creator>Chou, Alan Y.</creator><creator>Archdeacon, John</creator><creator>Kado, Clarence I.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>19980428</creationdate><title>Agrobacterium Transcriptional Regulator Ros is a Prokaryotic Zinc Finger Protein that Regulates the Plant Oncogene ipt</title><author>Chou, Alan Y. ; Archdeacon, John ; Kado, Clarence I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-d20dc15f3d9e312da38a6ca9a33301cc4b18b1edf0dc0f2fe69c2052d455d9203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Agrobacterium</topic><topic>Agrobacterium tumefaciens - enzymology</topic><topic>Alkyl and Aryl Transferases - genetics</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Bacterial Proteins</topic><topic>Binding sites</topic><topic>Biological Sciences</topic><topic>Cytokinins</topic><topic>DNA</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - physiology</topic><topic>Flowers & plants</topic><topic>Gels</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Isopentenyladenosine - metabolism</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Site-Directed</topic><topic>Oncogenes</topic><topic>Plant growth</topic><topic>Promoter Regions, Genetic</topic><topic>Proteins</topic><topic>Repressor Proteins - physiology</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Transcription, Genetic</topic><topic>Transcriptional regulatory elements</topic><topic>Transferases - genetics</topic><topic>Zinc</topic><topic>Zinc Fingers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chou, Alan Y.</creatorcontrib><creatorcontrib>Archdeacon, John</creatorcontrib><creatorcontrib>Kado, Clarence I.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chou, Alan Y.</au><au>Archdeacon, John</au><au>Kado, Clarence I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Agrobacterium Transcriptional Regulator Ros is a Prokaryotic Zinc Finger Protein that Regulates the Plant Oncogene ipt</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1998-04-28</date><risdate>1998</risdate><volume>95</volume><issue>9</issue><spage>5293</spage><epage>5298</epage><pages>5293-5298</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Virulence genes of Agrobacterium tumefaciens are under the control of positive and negative transcriptional regulators. We found that the transcriptional regulator Ros controls expression of the plant oncogene ipt, which encodes isopentenyl transferase, in A. tumefaciens. This enzyme is involved in biosynthesis of the plant growth hormone cytokinin in the host plant. An ipt promoter::cat reporter gene fusion showed a 10-fold increase in ipt promoter activity in A. tumefaciens ros mutant strains when compared with wild type. Also, increased levels (10-to 20-fold) of isopentenyl adenosine, the product of the reaction catalyzed by isopentenyl transferase, were detected in ros mutant strains. In vitro studies using purified Ros showed it binds directly to the ipt promoter. Analysis of the deduced Ros amino acid sequence identified a novel type of C2H2zinc finger. In Ros the peptide loop spacing of the zinc finger is 9 amino acids as opposed to the invariant 12 amino acids in the classical C2H2motif. Site-directed mutagenesis of Cys-82 and His-92 in this motif showed that these residues are essential for Zn2+and DNA binding activities of Ros. The existence of such a regulator in Agrobacterium may be due to horizontal interkingdom retrotransfer of the ros gene from plant to bacteria.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>9560269</pmid><doi>10.1073/pnas.95.9.5293</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1998-04, Vol.95 (9), p.5293-5298 |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Agrobacterium Agrobacterium tumefaciens - enzymology Alkyl and Aryl Transferases - genetics Amino Acid Sequence Amino acids Bacterial Proteins Binding sites Biological Sciences Cytokinins DNA DNA-Binding Proteins - genetics DNA-Binding Proteins - physiology Flowers & plants Gels Gene Expression Regulation, Bacterial Gene Expression Regulation, Plant Genes Isopentenyladenosine - metabolism Microbiology Molecular Sequence Data Mutagenesis, Site-Directed Oncogenes Plant growth Promoter Regions, Genetic Proteins Repressor Proteins - physiology Sequence Alignment Sequence Homology, Amino Acid Transcription, Genetic Transcriptional regulatory elements Transferases - genetics Zinc Zinc Fingers |
| title | Agrobacterium Transcriptional Regulator Ros is a Prokaryotic Zinc Finger Protein that Regulates the Plant Oncogene ipt |
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