Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E.coli

The Calvin Cycle is the primary conduit for the fixation of carbon dioxide into the biosphere; ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzes the rate-limiting fixation step. Our goal is to direct the evolution of RuBisCO variants with improved kinetic and biophysical properties...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Protein engineering, design and selection design and selection, 2006-03, Vol.19 (3), p.113-119
Hauptverfasser:	Parikh, Monal R., Greene, Dina N., Woods, Kristen K., Matsumura, Ichiro
Format:	Artikel
Sprache:	eng
Schlagworte:	5-bisphosphate carboxylase oxygenase Blotting, Western Carbon Dioxide - metabolism carbon dioxide fixation Cloning, Molecular Directed Molecular Evolution DNA, Bacterial Escherichia coli Escherichia coli - genetics Gene Transfer, Horizontal Genes, Bacterial Genetic Engineering - methods Genetic Variation horizontal transfer in vitro evolution Kinetics metabolic engineering Mutation ribulose 1 Ribulose-Bisphosphate Carboxylase - chemistry Ribulose-Bisphosphate Carboxylase - genetics Ribulose-Bisphosphate Carboxylase - isolation & purification Ribulose-Bisphosphate Carboxylase - metabolism Selection, Genetic Sequence Analysis, DNA Species Specificity Synechococcus Synechococcus - enzymology Synechococcus - growth & development
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!