Reverse transcriptase activity and particles of retroviral density in cultured canine lymphosarcoma supernatants
Lymphoid tissue from 43 cases of canine lymphosarcoma and from 40 clinically normal dogs have been examined for markers of retrovirus infection. From 69-76% of culture supernatants from lymphosarcomas were shown to contain particles of retroviral density and to possess poly rC-oligo dG templated pol...
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| Veröffentlicht in: | British journal of cancer 1983-02, Vol.47 (2), p.277-284 |
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| container_title | British journal of cancer |
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| creator | Tomley, F M Armstrong, S J Mahy, B W Owen, L N |
| description | Lymphoid tissue from 43 cases of canine lymphosarcoma and from 40 clinically normal dogs have been examined for markers of retrovirus infection. From 69-76% of culture supernatants from lymphosarcomas were shown to contain particles of retroviral density and to possess poly rC-oligo dG templated polymerase (reverse transcriptase) activity compared with 17-24% of culture supernatants from normal canine lymphoid cells. In 6 culture supernatants from cases of lymphosarcoma, high molecular weight 60-70S RNA was detected and shown to be found in association with this particulate reverse transcriptase activity. No such RNA was detected in 6 culture supernatants from normal canine lymphoid cells. |
| doi_str_mv | 10.1038/bjc.1983.36 |
| format | Article |
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No such RNA was detected in 6 culture supernatants from normal canine lymphoid cells.</description><identifier>ISSN: 0007-0920</identifier><identifier>EISSN: 1532-1827</identifier><identifier>DOI: 10.1038/bjc.1983.36</identifier><identifier>PMID: 6186265</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; Cancer Research ; Centrifugation, Density Gradient ; Dog Diseases - enzymology ; Dog Diseases - microbiology ; Dogs ; Drug Resistance ; Epidemiology ; Female ; Lymph Nodes - enzymology ; Lymph Nodes - microbiology ; Lymphoma, Non-Hodgkin - enzymology ; Lymphoma, Non-Hodgkin - microbiology ; Lymphoma, Non-Hodgkin - veterinary ; Male ; Molecular Medicine ; Oncology ; original-article ; Retroviridae - isolation & purification ; RNA, Neoplasm - analysis ; RNA-Directed DNA Polymerase - metabolism</subject><ispartof>British journal of cancer, 1983-02, Vol.47 (2), p.277-284</ispartof><rights>Cancer Research Campaign 1983</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-772c24df21ba8cdffad5bb48eda4644ebb35be506db9470f75bbb5a458aa72fe3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011285/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2011285/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,41467,42536,51297,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6186265$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tomley, F M</creatorcontrib><creatorcontrib>Armstrong, S J</creatorcontrib><creatorcontrib>Mahy, B W</creatorcontrib><creatorcontrib>Owen, L N</creatorcontrib><title>Reverse transcriptase activity and particles of retroviral density in cultured canine lymphosarcoma supernatants</title><title>British journal of cancer</title><addtitle>Br J Cancer</addtitle><addtitle>Br J Cancer</addtitle><description>Lymphoid tissue from 43 cases of canine lymphosarcoma and from 40 clinically normal dogs have been examined for markers of retrovirus infection. From 69-76% of culture supernatants from lymphosarcomas were shown to contain particles of retroviral density and to possess poly rC-oligo dG templated polymerase (reverse transcriptase) activity compared with 17-24% of culture supernatants from normal canine lymphoid cells. In 6 culture supernatants from cases of lymphosarcoma, high molecular weight 60-70S RNA was detected and shown to be found in association with this particulate reverse transcriptase activity. No such RNA was detected in 6 culture supernatants from normal canine lymphoid cells.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer Research</subject><subject>Centrifugation, Density Gradient</subject><subject>Dog Diseases - enzymology</subject><subject>Dog Diseases - microbiology</subject><subject>Dogs</subject><subject>Drug Resistance</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Lymph Nodes - enzymology</subject><subject>Lymph Nodes - microbiology</subject><subject>Lymphoma, Non-Hodgkin - enzymology</subject><subject>Lymphoma, Non-Hodgkin - microbiology</subject><subject>Lymphoma, Non-Hodgkin - veterinary</subject><subject>Male</subject><subject>Molecular Medicine</subject><subject>Oncology</subject><subject>original-article</subject><subject>Retroviridae - isolation & purification</subject><subject>RNA, Neoplasm - analysis</subject><subject>RNA-Directed DNA Polymerase - metabolism</subject><issn>0007-0920</issn><issn>1532-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo67h68izk5EV7TNKdTuYiyOIXLAii51BJV-9m6E7aJD0w_94MMywKgqdQPA8vqXoJecnZlrNWv7N7t-U73W7b_hHZcNmKhmuhHpMNY0w1bCfYU_Is530dd0yrK3LVc92LXm7I8h0PmDLSkiBkl_xSoE7gij_4cqQQBrpAKt5NmGkcacKS4sEnmOiAIZ8cH6hbp7ImHKiD4APS6Tgv9zFDcnEGmtcFU4ACoeTn5MkIU8YXl_ea_Pz08cfNl-b22-evNx9uG9d1XWmUEk50wyi4Be2GcYRBWttpHKDruw6tbaVFyfrB7jrFRlWpldBJDaDEiO01eX_OXVY74-Aw1A0nsyQ_QzqaCN78TYK_N3fxYATjXGhZA15fAlL8tWIuZvbZ4TRBwLhmo1mreL9j_xW5lFJppqr45iy6FHNOOD78hjNzatLUJs2pSdP21X715wIP7qW6yt-eea4k3GEy-7jWK0_5n3G_AWhjriY</recordid><startdate>19830201</startdate><enddate>19830201</enddate><creator>Tomley, F M</creator><creator>Armstrong, S J</creator><creator>Mahy, B W</creator><creator>Owen, L N</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19830201</creationdate><title>Reverse transcriptase activity and particles of retroviral density in cultured canine lymphosarcoma supernatants</title><author>Tomley, F M ; Armstrong, S J ; Mahy, B W ; Owen, L N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-772c24df21ba8cdffad5bb48eda4644ebb35be506db9470f75bbb5a458aa72fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cancer Research</topic><topic>Centrifugation, Density Gradient</topic><topic>Dog Diseases - enzymology</topic><topic>Dog Diseases - microbiology</topic><topic>Dogs</topic><topic>Drug Resistance</topic><topic>Epidemiology</topic><topic>Female</topic><topic>Lymph Nodes - enzymology</topic><topic>Lymph Nodes - microbiology</topic><topic>Lymphoma, Non-Hodgkin - enzymology</topic><topic>Lymphoma, Non-Hodgkin - microbiology</topic><topic>Lymphoma, Non-Hodgkin - veterinary</topic><topic>Male</topic><topic>Molecular Medicine</topic><topic>Oncology</topic><topic>original-article</topic><topic>Retroviridae - isolation & purification</topic><topic>RNA, Neoplasm - analysis</topic><topic>RNA-Directed DNA Polymerase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tomley, F M</creatorcontrib><creatorcontrib>Armstrong, S J</creatorcontrib><creatorcontrib>Mahy, B W</creatorcontrib><creatorcontrib>Owen, L N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tomley, F M</au><au>Armstrong, S J</au><au>Mahy, B W</au><au>Owen, L N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reverse transcriptase activity and particles of retroviral density in cultured canine lymphosarcoma supernatants</atitle><jtitle>British journal of cancer</jtitle><stitle>Br J Cancer</stitle><addtitle>Br J Cancer</addtitle><date>1983-02-01</date><risdate>1983</risdate><volume>47</volume><issue>2</issue><spage>277</spage><epage>284</epage><pages>277-284</pages><issn>0007-0920</issn><eissn>1532-1827</eissn><abstract>Lymphoid tissue from 43 cases of canine lymphosarcoma and from 40 clinically normal dogs have been examined for markers of retrovirus infection. From 69-76% of culture supernatants from lymphosarcomas were shown to contain particles of retroviral density and to possess poly rC-oligo dG templated polymerase (reverse transcriptase) activity compared with 17-24% of culture supernatants from normal canine lymphoid cells. In 6 culture supernatants from cases of lymphosarcoma, high molecular weight 60-70S RNA was detected and shown to be found in association with this particulate reverse transcriptase activity. No such RNA was detected in 6 culture supernatants from normal canine lymphoid cells.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>6186265</pmid><doi>10.1038/bjc.1983.36</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; SpringerLink Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Nature Journals Online; PubMed Central |
| subjects | Animals Biomedical and Life Sciences Biomedicine Cancer Research Centrifugation, Density Gradient Dog Diseases - enzymology Dog Diseases - microbiology Dogs Drug Resistance Epidemiology Female Lymph Nodes - enzymology Lymph Nodes - microbiology Lymphoma, Non-Hodgkin - enzymology Lymphoma, Non-Hodgkin - microbiology Lymphoma, Non-Hodgkin - veterinary Male Molecular Medicine Oncology original-article Retroviridae - isolation & purification RNA, Neoplasm - analysis RNA-Directed DNA Polymerase - metabolism |
| title | Reverse transcriptase activity and particles of retroviral density in cultured canine lymphosarcoma supernatants |
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