Quantitative cytochemical assessment of the neurotoxicity of misonidazole in the mouse
A quantitative, cytochemical assay for measuring lysosomal enzymes in the peripheral nerves of mice has been developed. That the time course of lysosomal enzyme changes after misonidazole (MISO) treatment reflects the degree of neurotoxicity of this agent in the mouse, has been confirmed by the use...
Gespeichert in:
| Veröffentlicht in: | British journal of cancer 1982-04, Vol.45 (4), p.582-587 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 587 |
|---|---|
| container_issue | 4 |
| container_start_page | 582 |
| container_title | British journal of cancer |
| container_volume | 45 |
| creator | Clarke, C Dawson, K B Sheldon, P W |
| description | A quantitative, cytochemical assay for measuring lysosomal enzymes in the peripheral nerves of mice has been developed. That the time course of lysosomal enzyme changes after misonidazole (MISO) treatment reflects the degree of neurotoxicity of this agent in the mouse, has been confirmed by the use of two known neurotoxic compounds: methyl mercury and acrylamide. This effect is specific to the peripheral nerves and was not found in liver, kidney, heart or cerebral cortex. Enzyme activities varied with mouse strain and sex, as did the response to MISO treatment. Of the mice studied, female C57 gave the greatest increase in beta-glucuronidase activity. With the MISO dose of 0.6 mg/g/dose the increased enzyme activity was independent of the route of administration and appeared to approach a plateau after 5 daily doses. |
| doi_str_mv | 10.1038/bjc.1982.95 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2010990</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>7073948</sourcerecordid><originalsourceid>FETCH-LOGICAL-c395t-1b62786e85ee6980e8b9ea22bb7233d8da55f5a14f1ea8333cdf66a0aa5b252c3</originalsourceid><addsrcrecordid>eNp1kM9LHDEYhkNRdGt76lmcu86aH2YmuQhFtC0IIrS9hm8y37hZdhJJMku3f73Zriz24Cl8eZ_vTXgI-cLonFGhLrulnTOt-FzLD2TGpOA1U7w9IDNKaVtTzekx-ZjSsoyaqvaIHLW0FfpKzcjvxwl8dhmyW2NlNznYBY7OwqqClDClEX2uwlDlBVYepxhy-OOsy5vt5ehS8K6Hv2GFlfP_oDFMCT-RwwFWCT-_nifk193tz5vv9f3Dtx83X-9rK7TMNesa3qoGlURstKKoOo3Aede1XIhe9SDlIIFdDQxBCSFsPzQNUADZccmtOCHXu97nqRuxt-WzEVbmOboR4sYEcOb_xLuFeQprwymjWtNScL4rsDGkFHHY7zJqtnZNsWu2do2WhT59-9yefdVZ8otdnkrinzCaZZiiLwbeqTvb4R7yFHFfV5gtUogXzJaSuw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Quantitative cytochemical assessment of the neurotoxicity of misonidazole in the mouse</title><source>MEDLINE</source><source>Nature</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>SpringerLink Journals - AutoHoldings</source><creator>Clarke, C ; Dawson, K B ; Sheldon, P W</creator><creatorcontrib>Clarke, C ; Dawson, K B ; Sheldon, P W</creatorcontrib><description>A quantitative, cytochemical assay for measuring lysosomal enzymes in the peripheral nerves of mice has been developed. That the time course of lysosomal enzyme changes after misonidazole (MISO) treatment reflects the degree of neurotoxicity of this agent in the mouse, has been confirmed by the use of two known neurotoxic compounds: methyl mercury and acrylamide. This effect is specific to the peripheral nerves and was not found in liver, kidney, heart or cerebral cortex. Enzyme activities varied with mouse strain and sex, as did the response to MISO treatment. Of the mice studied, female C57 gave the greatest increase in beta-glucuronidase activity. With the MISO dose of 0.6 mg/g/dose the increased enzyme activity was independent of the route of administration and appeared to approach a plateau after 5 daily doses.</description><identifier>ISSN: 0007-0920</identifier><identifier>EISSN: 1532-1827</identifier><identifier>DOI: 10.1038/bjc.1982.95</identifier><identifier>PMID: 7073948</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Acrylamide ; Acrylamides - pharmacology ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Cancer Research ; Drug Resistance ; Epidemiology ; Female ; Glucuronidase - metabolism ; Male ; Methylmercury Compounds - pharmacology ; Mice ; Mice, Inbred Strains ; Misonidazole - administration & dosage ; Misonidazole - pharmacology ; Molecular Medicine ; Nitroimidazoles - pharmacology ; Oncology ; original-article ; Sex Factors ; Tibial Nerve - drug effects ; Tibial Nerve - enzymology</subject><ispartof>British journal of cancer, 1982-04, Vol.45 (4), p.582-587</ispartof><rights>Cancer Research Campaign 1982</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010990/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010990/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,2727,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7073948$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clarke, C</creatorcontrib><creatorcontrib>Dawson, K B</creatorcontrib><creatorcontrib>Sheldon, P W</creatorcontrib><title>Quantitative cytochemical assessment of the neurotoxicity of misonidazole in the mouse</title><title>British journal of cancer</title><addtitle>Br J Cancer</addtitle><addtitle>Br J Cancer</addtitle><description>A quantitative, cytochemical assay for measuring lysosomal enzymes in the peripheral nerves of mice has been developed. That the time course of lysosomal enzyme changes after misonidazole (MISO) treatment reflects the degree of neurotoxicity of this agent in the mouse, has been confirmed by the use of two known neurotoxic compounds: methyl mercury and acrylamide. This effect is specific to the peripheral nerves and was not found in liver, kidney, heart or cerebral cortex. Enzyme activities varied with mouse strain and sex, as did the response to MISO treatment. Of the mice studied, female C57 gave the greatest increase in beta-glucuronidase activity. With the MISO dose of 0.6 mg/g/dose the increased enzyme activity was independent of the route of administration and appeared to approach a plateau after 5 daily doses.</description><subject>Acrylamide</subject><subject>Acrylamides - pharmacology</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer Research</subject><subject>Drug Resistance</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Glucuronidase - metabolism</subject><subject>Male</subject><subject>Methylmercury Compounds - pharmacology</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Misonidazole - administration & dosage</subject><subject>Misonidazole - pharmacology</subject><subject>Molecular Medicine</subject><subject>Nitroimidazoles - pharmacology</subject><subject>Oncology</subject><subject>original-article</subject><subject>Sex Factors</subject><subject>Tibial Nerve - drug effects</subject><subject>Tibial Nerve - enzymology</subject><issn>0007-0920</issn><issn>1532-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM9LHDEYhkNRdGt76lmcu86aH2YmuQhFtC0IIrS9hm8y37hZdhJJMku3f73Zriz24Cl8eZ_vTXgI-cLonFGhLrulnTOt-FzLD2TGpOA1U7w9IDNKaVtTzekx-ZjSsoyaqvaIHLW0FfpKzcjvxwl8dhmyW2NlNznYBY7OwqqClDClEX2uwlDlBVYepxhy-OOsy5vt5ehS8K6Hv2GFlfP_oDFMCT-RwwFWCT-_nifk193tz5vv9f3Dtx83X-9rK7TMNesa3qoGlURstKKoOo3Aede1XIhe9SDlIIFdDQxBCSFsPzQNUADZccmtOCHXu97nqRuxt-WzEVbmOboR4sYEcOb_xLuFeQprwymjWtNScL4rsDGkFHHY7zJqtnZNsWu2do2WhT59-9yefdVZ8otdnkrinzCaZZiiLwbeqTvb4R7yFHFfV5gtUogXzJaSuw</recordid><startdate>19820401</startdate><enddate>19820401</enddate><creator>Clarke, C</creator><creator>Dawson, K B</creator><creator>Sheldon, P W</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19820401</creationdate><title>Quantitative cytochemical assessment of the neurotoxicity of misonidazole in the mouse</title><author>Clarke, C ; Dawson, K B ; Sheldon, P W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-1b62786e85ee6980e8b9ea22bb7233d8da55f5a14f1ea8333cdf66a0aa5b252c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Acrylamide</topic><topic>Acrylamides - pharmacology</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cancer Research</topic><topic>Drug Resistance</topic><topic>Epidemiology</topic><topic>Female</topic><topic>Glucuronidase - metabolism</topic><topic>Male</topic><topic>Methylmercury Compounds - pharmacology</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Misonidazole - administration & dosage</topic><topic>Misonidazole - pharmacology</topic><topic>Molecular Medicine</topic><topic>Nitroimidazoles - pharmacology</topic><topic>Oncology</topic><topic>original-article</topic><topic>Sex Factors</topic><topic>Tibial Nerve - drug effects</topic><topic>Tibial Nerve - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clarke, C</creatorcontrib><creatorcontrib>Dawson, K B</creatorcontrib><creatorcontrib>Sheldon, P W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clarke, C</au><au>Dawson, K B</au><au>Sheldon, P W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative cytochemical assessment of the neurotoxicity of misonidazole in the mouse</atitle><jtitle>British journal of cancer</jtitle><stitle>Br J Cancer</stitle><addtitle>Br J Cancer</addtitle><date>1982-04-01</date><risdate>1982</risdate><volume>45</volume><issue>4</issue><spage>582</spage><epage>587</epage><pages>582-587</pages><issn>0007-0920</issn><eissn>1532-1827</eissn><abstract>A quantitative, cytochemical assay for measuring lysosomal enzymes in the peripheral nerves of mice has been developed. That the time course of lysosomal enzyme changes after misonidazole (MISO) treatment reflects the degree of neurotoxicity of this agent in the mouse, has been confirmed by the use of two known neurotoxic compounds: methyl mercury and acrylamide. This effect is specific to the peripheral nerves and was not found in liver, kidney, heart or cerebral cortex. Enzyme activities varied with mouse strain and sex, as did the response to MISO treatment. Of the mice studied, female C57 gave the greatest increase in beta-glucuronidase activity. With the MISO dose of 0.6 mg/g/dose the increased enzyme activity was independent of the route of administration and appeared to approach a plateau after 5 daily doses.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>7073948</pmid><doi>10.1038/bjc.1982.95</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-0920 |
| ispartof | British journal of cancer, 1982-04, Vol.45 (4), p.582-587 |
| issn | 0007-0920 1532-1827 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2010990 |
| source | MEDLINE; Nature; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; SpringerLink Journals - AutoHoldings |
| subjects | Acrylamide Acrylamides - pharmacology Animals Biomedical and Life Sciences Biomedicine Cancer Research Drug Resistance Epidemiology Female Glucuronidase - metabolism Male Methylmercury Compounds - pharmacology Mice Mice, Inbred Strains Misonidazole - administration & dosage Misonidazole - pharmacology Molecular Medicine Nitroimidazoles - pharmacology Oncology original-article Sex Factors Tibial Nerve - drug effects Tibial Nerve - enzymology |
| title | Quantitative cytochemical assessment of the neurotoxicity of misonidazole in the mouse |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T17%3A09%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantitative%20cytochemical%20assessment%20of%20the%20neurotoxicity%20of%20misonidazole%20in%20the%20mouse&rft.jtitle=British%20journal%20of%20cancer&rft.au=Clarke,%20C&rft.date=1982-04-01&rft.volume=45&rft.issue=4&rft.spage=582&rft.epage=587&rft.pages=582-587&rft.issn=0007-0920&rft.eissn=1532-1827&rft_id=info:doi/10.1038/bjc.1982.95&rft_dat=%3Cpubmed_cross%3E7073948%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/7073948&rfr_iscdi=true |