Detection of human papillomavirus DNA in biopsies of human oral tissue
We have employed molecular probes produced from DNA fragments of human papillomavirus, cloned into prokaryotic vectors, to detect virus nucleic acid sequences in extracts of human oral tissues. The study was conducted with duplicate coded snap-frozen tissue biopsies from which frozen sections had be...
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| Veröffentlicht in: | British journal of cancer 1987-09, Vol.56 (3), p.245-250 |
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| container_title | British journal of cancer |
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| creator | Maitland, NJ Cox, MF Lynas, C Prime, SS Meanwell, CA Scully, C |
| description | We have employed molecular probes produced from DNA fragments of human papillomavirus, cloned into prokaryotic vectors, to detect virus nucleic acid sequences in extracts of human oral tissues. The study was conducted with duplicate coded snap-frozen tissue biopsies from which frozen sections had been taken to accurately assess the pathology of each particular sample. The results show that a large proportion of the oral biopsies contained DNA which hybridized to the viral DNA probes, even under conditions of high stringency. The presence of virus did not correlate with neoplasia in the tissues examined, but HPV like sequences were found in a high proportion (80%) of biopsies taken from areas of keratosis and lichen planus and also in 41 to 46% of normal and tumour tissues. |
| doi_str_mv | 10.1038/bjc.1987.185 |
| format | Article |
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The study was conducted with duplicate coded snap-frozen tissue biopsies from which frozen sections had been taken to accurately assess the pathology of each particular sample. The results show that a large proportion of the oral biopsies contained DNA which hybridized to the viral DNA probes, even under conditions of high stringency. The presence of virus did not correlate with neoplasia in the tissues examined, but HPV like sequences were found in a high proportion (80%) of biopsies taken from areas of keratosis and lichen planus and also in 41 to 46% of normal and tumour tissues.</description><identifier>ISSN: 0007-0920</identifier><identifier>EISSN: 1532-1827</identifier><identifier>DOI: 10.1038/bjc.1987.185</identifier><identifier>PMID: 2822070</identifier><identifier>CODEN: BJCAAI</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Biopsy ; Cancer Research ; Carcinoma, Squamous Cell - microbiology ; Deoxyribonucleotides - analysis ; DNA, Viral - analysis ; Drug Resistance ; Epidemiology ; experimental-oncology ; Humans ; Lichen Planus - microbiology ; Medical sciences ; Molecular Medicine ; Mouth - microbiology ; Mouth Diseases - microbiology ; Mouth Neoplasms - microbiology ; Nucleic Acid Hybridization ; Oncology ; Otorhinolaryngology. Stomatology ; Papillomaviridae - isolation & purification ; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><ispartof>British journal of cancer, 1987-09, Vol.56 (3), p.245-250</ispartof><rights>Cancer Research Campaign 1987</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c537t-5f69945c61b735c796a7bdcd469115884d810c31adbbe4ac0f4d0be7dc561a1d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2002211/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2002211/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,2727,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8336038$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2822070$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maitland, NJ</creatorcontrib><creatorcontrib>Cox, MF</creatorcontrib><creatorcontrib>Lynas, C</creatorcontrib><creatorcontrib>Prime, SS</creatorcontrib><creatorcontrib>Meanwell, CA</creatorcontrib><creatorcontrib>Scully, C</creatorcontrib><title>Detection of human papillomavirus DNA in biopsies of human oral tissue</title><title>British journal of cancer</title><addtitle>Br J Cancer</addtitle><addtitle>Br J Cancer</addtitle><description>We have employed molecular probes produced from DNA fragments of human papillomavirus, cloned into prokaryotic vectors, to detect virus nucleic acid sequences in extracts of human oral tissues. The study was conducted with duplicate coded snap-frozen tissue biopsies from which frozen sections had been taken to accurately assess the pathology of each particular sample. The results show that a large proportion of the oral biopsies contained DNA which hybridized to the viral DNA probes, even under conditions of high stringency. The presence of virus did not correlate with neoplasia in the tissues examined, but HPV like sequences were found in a high proportion (80%) of biopsies taken from areas of keratosis and lichen planus and also in 41 to 46% of normal and tumour tissues.</description><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biopsy</subject><subject>Cancer Research</subject><subject>Carcinoma, Squamous Cell - microbiology</subject><subject>Deoxyribonucleotides - analysis</subject><subject>DNA, Viral - analysis</subject><subject>Drug Resistance</subject><subject>Epidemiology</subject><subject>experimental-oncology</subject><subject>Humans</subject><subject>Lichen Planus - microbiology</subject><subject>Medical sciences</subject><subject>Molecular Medicine</subject><subject>Mouth - microbiology</subject><subject>Mouth Diseases - microbiology</subject><subject>Mouth Neoplasms - microbiology</subject><subject>Nucleic Acid Hybridization</subject><subject>Oncology</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Papillomaviridae - isolation & purification</subject><subject>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><issn>0007-0920</issn><issn>1532-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1LwzAchoMoc37cvAo9iCc7k7Rp0oswNqfC0IueQ5qmW0ab1KQd-N-b0TH14CmE9-F9fzwAXCE4QTBh98VGTlDO6AQxcgTGiCQ4RgzTYzCGENIY5hiegjPvN-GbQ0ZHYIQZxpDCMVjMVadkp62JbBWt-0aYqBWtrmvbiK12vY_mr9NIm6jQtvVa-R_OOlFHnfa-VxfgpBK1V5f79xx8LB7fZ8_x8u3pZTZdxpIktItJleV5SmSGCpoQSfNM0KKUZZrlCBHG0pIhKBMkyqJQqZCwSktYKFpKkiGByuQcPAy9bV80qpTKdOEI3jrdCPfFrdD8b2L0mq_slmMIMUYoFNzuC5z97JXveKO9VHUtjLK952GfYEazAN4NoHTWe6eqwwiCfOedB-98550H7wG__n3YAd6LDvnNPhdeirpywkjtDxhLkix0BiweMB8Ss1KOb2zvTFD632w08EZ0vVOHvgDtmB3yDY_Fpdg</recordid><startdate>19870901</startdate><enddate>19870901</enddate><creator>Maitland, NJ</creator><creator>Cox, MF</creator><creator>Lynas, C</creator><creator>Prime, SS</creator><creator>Meanwell, CA</creator><creator>Scully, C</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19870901</creationdate><title>Detection of human papillomavirus DNA in biopsies of human oral tissue</title><author>Maitland, NJ ; Cox, MF ; Lynas, C ; Prime, SS ; Meanwell, CA ; Scully, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c537t-5f69945c61b735c796a7bdcd469115884d810c31adbbe4ac0f4d0be7dc561a1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biopsy</topic><topic>Cancer Research</topic><topic>Carcinoma, Squamous Cell - microbiology</topic><topic>Deoxyribonucleotides - analysis</topic><topic>DNA, Viral - analysis</topic><topic>Drug Resistance</topic><topic>Epidemiology</topic><topic>experimental-oncology</topic><topic>Humans</topic><topic>Lichen Planus - microbiology</topic><topic>Medical sciences</topic><topic>Molecular Medicine</topic><topic>Mouth - microbiology</topic><topic>Mouth Diseases - microbiology</topic><topic>Mouth Neoplasms - microbiology</topic><topic>Nucleic Acid Hybridization</topic><topic>Oncology</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Papillomaviridae - isolation & purification</topic><topic>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maitland, NJ</creatorcontrib><creatorcontrib>Cox, MF</creatorcontrib><creatorcontrib>Lynas, C</creatorcontrib><creatorcontrib>Prime, SS</creatorcontrib><creatorcontrib>Meanwell, CA</creatorcontrib><creatorcontrib>Scully, C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maitland, NJ</au><au>Cox, MF</au><au>Lynas, C</au><au>Prime, SS</au><au>Meanwell, CA</au><au>Scully, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of human papillomavirus DNA in biopsies of human oral tissue</atitle><jtitle>British journal of cancer</jtitle><stitle>Br J Cancer</stitle><addtitle>Br J Cancer</addtitle><date>1987-09-01</date><risdate>1987</risdate><volume>56</volume><issue>3</issue><spage>245</spage><epage>250</epage><pages>245-250</pages><issn>0007-0920</issn><eissn>1532-1827</eissn><coden>BJCAAI</coden><abstract>We have employed molecular probes produced from DNA fragments of human papillomavirus, cloned into prokaryotic vectors, to detect virus nucleic acid sequences in extracts of human oral tissues. The study was conducted with duplicate coded snap-frozen tissue biopsies from which frozen sections had been taken to accurately assess the pathology of each particular sample. The results show that a large proportion of the oral biopsies contained DNA which hybridized to the viral DNA probes, even under conditions of high stringency. The presence of virus did not correlate with neoplasia in the tissues examined, but HPV like sequences were found in a high proportion (80%) of biopsies taken from areas of keratosis and lichen planus and also in 41 to 46% of normal and tumour tissues.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>2822070</pmid><doi>10.1038/bjc.1987.185</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Nature; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Biological and medical sciences Biomedical and Life Sciences Biomedicine Biopsy Cancer Research Carcinoma, Squamous Cell - microbiology Deoxyribonucleotides - analysis DNA, Viral - analysis Drug Resistance Epidemiology experimental-oncology Humans Lichen Planus - microbiology Medical sciences Molecular Medicine Mouth - microbiology Mouth Diseases - microbiology Mouth Neoplasms - microbiology Nucleic Acid Hybridization Oncology Otorhinolaryngology. Stomatology Papillomaviridae - isolation & purification Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology |
| title | Detection of human papillomavirus DNA in biopsies of human oral tissue |
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