Near infrared multiphoton-induced generation and detection of hydroxyl radicals in a biochemical system

Solutions of tryptophan and N-hydroxypyridine-2-thione (mercaptopyridine-N-oxide, MPNO) were irradiated at 335nm. Formation of 5-hydroxytryptophan was inferred from increased fluorescence at 334nm on excitation at 315nm, conditions chosen for selective detection of 5-hydroxytryptophan. Such experime...

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Veröffentlicht in:	Archives of biochemistry and biophysics 2007-08, Vol.464 (2), p.314-321
Hauptverfasser:	Botchway, Stanley W., Crisostomo, Ana G., Parker, Anthony W., Bisby, Roger H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry - methods Complex Mixtures - analysis Complex Mixtures - chemistry Femtosecond Hydroxyl Hydroxyl Radical - analysis Laser Mercaptopyridine-N-oxide Microscopy Microscopy, Fluorescence, Multiphoton - methods Multiphoton PhotoFenton Pyridines - analysis Pyridines - chemistry Radical Scavenging Sensitivity and Specificity Spectrophotometry, Infrared - methods Spectroscopy Tryptophan - analysis Tryptophan - chemistry
Online-Zugang:	Volltext
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