Lack of prognostic significance of DNA ploidy and S phase fraction in breast cancer
DNA Ploidy and S phase fraction (SPF) were measured in Stage I and II breast cancers from patients with at least 8 years of follow-up, to assess the prognostic significance of these data. Disaggregated sections of formalin-fixed, paraffin-embedded tumour were analysed by flow cytometry. SPF was calc...
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| Veröffentlicht in: | British journal of cancer 1992-11, Vol.66 (5), p.925-929 |
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| creator | STANTON, P. D COOKE, T. G OAKES, S. J WINSTANLEY, J HOLT, S GEORGE, W. D MURRAY, G. D |
| description | DNA Ploidy and S phase fraction (SPF) were measured in Stage I and II breast cancers from patients with at least 8 years of follow-up, to assess the prognostic significance of these data. Disaggregated sections of formalin-fixed, paraffin-embedded tumour were analysed by flow cytometry. SPF was calculated using a rectangular model of S phase, after subtraction of background debris using an exponential model. 64% of tumours were DNA aneuploid. The median SPF was 4.5% for DNA diploid, and 10.9% for DNA aneuploid tumours. There were small reductions in survival at 10 years for DNA aneuploid tumours, and for tumours with above median SPF, but these were not statistically significant. The relative hazard for DNA aneuploid tumours was 1.20 (95% CI 0.81-1.76), and for high SPF was 1.31 (95% CI 0.87-1.98). Neither factor was statistically correlated with survival in multivariate analysis. Technical and theoretical factors limit the accuracy and reproducibility of flow cytometric data, and may explain the lack of prognostic information given. |
| doi_str_mv | 10.1038/bjc.1992.387 |
| format | Article |
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D ; COOKE, T. G ; OAKES, S. J ; WINSTANLEY, J ; HOLT, S ; GEORGE, W. D ; MURRAY, G. D</creator><creatorcontrib>STANTON, P. D ; COOKE, T. G ; OAKES, S. J ; WINSTANLEY, J ; HOLT, S ; GEORGE, W. D ; MURRAY, G. D</creatorcontrib><description>DNA Ploidy and S phase fraction (SPF) were measured in Stage I and II breast cancers from patients with at least 8 years of follow-up, to assess the prognostic significance of these data. Disaggregated sections of formalin-fixed, paraffin-embedded tumour were analysed by flow cytometry. SPF was calculated using a rectangular model of S phase, after subtraction of background debris using an exponential model. 64% of tumours were DNA aneuploid. The median SPF was 4.5% for DNA diploid, and 10.9% for DNA aneuploid tumours. There were small reductions in survival at 10 years for DNA aneuploid tumours, and for tumours with above median SPF, but these were not statistically significant. The relative hazard for DNA aneuploid tumours was 1.20 (95% CI 0.81-1.76), and for high SPF was 1.31 (95% CI 0.87-1.98). Neither factor was statistically correlated with survival in multivariate analysis. Technical and theoretical factors limit the accuracy and reproducibility of flow cytometric data, and may explain the lack of prognostic information given.</description><identifier>ISSN: 0007-0920</identifier><identifier>EISSN: 1532-1827</identifier><identifier>DOI: 10.1038/bjc.1992.387</identifier><identifier>PMID: 1419638</identifier><identifier>CODEN: BJCAAI</identifier><language>eng</language><publisher>Basingstoke: Nature Publishing Group</publisher><subject>Biological and medical sciences ; Breast Neoplasms - genetics ; Breast Neoplasms - pathology ; Cohort Studies ; DNA, Neoplasm - genetics ; Female ; Follow-Up Studies ; Gynecology. Andrology. 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D</creatorcontrib><creatorcontrib>COOKE, T. G</creatorcontrib><creatorcontrib>OAKES, S. J</creatorcontrib><creatorcontrib>WINSTANLEY, J</creatorcontrib><creatorcontrib>HOLT, S</creatorcontrib><creatorcontrib>GEORGE, W. D</creatorcontrib><creatorcontrib>MURRAY, G. D</creatorcontrib><title>Lack of prognostic significance of DNA ploidy and S phase fraction in breast cancer</title><title>British journal of cancer</title><addtitle>Br J Cancer</addtitle><description>DNA Ploidy and S phase fraction (SPF) were measured in Stage I and II breast cancers from patients with at least 8 years of follow-up, to assess the prognostic significance of these data. Disaggregated sections of formalin-fixed, paraffin-embedded tumour were analysed by flow cytometry. SPF was calculated using a rectangular model of S phase, after subtraction of background debris using an exponential model. 64% of tumours were DNA aneuploid. The median SPF was 4.5% for DNA diploid, and 10.9% for DNA aneuploid tumours. There were small reductions in survival at 10 years for DNA aneuploid tumours, and for tumours with above median SPF, but these were not statistically significant. The relative hazard for DNA aneuploid tumours was 1.20 (95% CI 0.81-1.76), and for high SPF was 1.31 (95% CI 0.87-1.98). Neither factor was statistically correlated with survival in multivariate analysis. Technical and theoretical factors limit the accuracy and reproducibility of flow cytometric data, and may explain the lack of prognostic information given.</description><subject>Biological and medical sciences</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - pathology</subject><subject>Cohort Studies</subject><subject>DNA, Neoplasm - genetics</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Mammary gland diseases</subject><subject>Medical sciences</subject><subject>Multivariate Analysis</subject><subject>Neoplasm Staging</subject><subject>Ploidies</subject><subject>Prognosis</subject><subject>S Phase - physiology</subject><subject>Tumors</subject><issn>0007-0920</issn><issn>1532-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1vEzEQxS1EVdLCjSuSD4hTN9ie9delUlVaQIrgUDhbXq83dbuxt_YGqf99HRK1cBqN3m_ejOYh9J6SJSWgPnd3bkm1ZktQ8hVaUA6soYrJ12hBCJEN0Yy8QSel3NVWEyWP0TFtqRagFuhmZd09TgOeclrHVObgcAnrGIbgbHR-J335cYGnMYX-EdvY4xs83dri8ZCtm0OKOETcZW_LjP-O5LfoaLBj8e8O9RT9vr76dfmtWf38-v3yYtW4lqi5EVqrXgolekYcFUSDVZxSqnsAoTvgXKtB9MLVq0EwaAVjkrfMOdu7jns4Red732nbbXzvfJyzHc2Uw8bmR5NsMP8rMdyadfpjqJZSa1oNPh0McnrY-jKbTSjOj6ONPm2LkQCECc4reLYHXU6lZD88L6HE7EIwNQSzC8HUECr-4d_DXuD916v-8aDb4uxYHxldKM9YC5prAHgCbb6OfA</recordid><startdate>19921101</startdate><enddate>19921101</enddate><creator>STANTON, P. 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G</creatorcontrib><creatorcontrib>OAKES, S. J</creatorcontrib><creatorcontrib>WINSTANLEY, J</creatorcontrib><creatorcontrib>HOLT, S</creatorcontrib><creatorcontrib>GEORGE, W. D</creatorcontrib><creatorcontrib>MURRAY, G. D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>STANTON, P. D</au><au>COOKE, T. G</au><au>OAKES, S. J</au><au>WINSTANLEY, J</au><au>HOLT, S</au><au>GEORGE, W. D</au><au>MURRAY, G. D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lack of prognostic significance of DNA ploidy and S phase fraction in breast cancer</atitle><jtitle>British journal of cancer</jtitle><addtitle>Br J Cancer</addtitle><date>1992-11-01</date><risdate>1992</risdate><volume>66</volume><issue>5</issue><spage>925</spage><epage>929</epage><pages>925-929</pages><issn>0007-0920</issn><eissn>1532-1827</eissn><coden>BJCAAI</coden><abstract>DNA Ploidy and S phase fraction (SPF) were measured in Stage I and II breast cancers from patients with at least 8 years of follow-up, to assess the prognostic significance of these data. Disaggregated sections of formalin-fixed, paraffin-embedded tumour were analysed by flow cytometry. SPF was calculated using a rectangular model of S phase, after subtraction of background debris using an exponential model. 64% of tumours were DNA aneuploid. The median SPF was 4.5% for DNA diploid, and 10.9% for DNA aneuploid tumours. There were small reductions in survival at 10 years for DNA aneuploid tumours, and for tumours with above median SPF, but these were not statistically significant. The relative hazard for DNA aneuploid tumours was 1.20 (95% CI 0.81-1.76), and for high SPF was 1.31 (95% CI 0.87-1.98). Neither factor was statistically correlated with survival in multivariate analysis. Technical and theoretical factors limit the accuracy and reproducibility of flow cytometric data, and may explain the lack of prognostic information given.</abstract><cop>Basingstoke</cop><pub>Nature Publishing Group</pub><pmid>1419638</pmid><doi>10.1038/bjc.1992.387</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Breast Neoplasms - genetics Breast Neoplasms - pathology Cohort Studies DNA, Neoplasm - genetics Female Follow-Up Studies Gynecology. Andrology. Obstetrics Humans Mammary gland diseases Medical sciences Multivariate Analysis Neoplasm Staging Ploidies Prognosis S Phase - physiology Tumors |
| title | Lack of prognostic significance of DNA ploidy and S phase fraction in breast cancer |
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