Isolation of Microarray-Grade Total RNA, MicroRNA, and DNA from a Single PAXgene Blood RNA Tube
We have developed a procedure for isolation of microRNA and genomic DNA in addition to total RNA from whole blood stabilized in PAXgene Blood RNA tubes. The procedure is based on automatic extraction on a BioRobot MDx and includes isolation of DNA from a fraction of the stabilized blood and recovery...
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| Veröffentlicht in: | The Journal of molecular diagnostics : JMD 2007-09, Vol.9 (4), p.452-458 |
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| container_title | The Journal of molecular diagnostics : JMD |
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| creator | Kruhøffer, Mogens Dyrskjøt, Lars Voss, Thorsten Lindberg, Raija L.P Wyrich, Ralf Thykjaer, Thomas Orntoft, Torben F |
| description | We have developed a procedure for isolation of microRNA and genomic DNA in addition to total RNA from whole blood stabilized in PAXgene Blood RNA tubes. The procedure is based on automatic extraction on a BioRobot MDx and includes isolation of DNA from a fraction of the stabilized blood and recovery of small RNA species that are otherwise lost. The procedure presented here is suitable for large-scale experiments and is amenable to further automation. Procured total RNA and DNA was tested using Affymetrix Expression and single-nucleotide polymorphism GeneChips, respectively, and isolated microRNA was tested using spotted locked nucleic acid-based microarrays. We conclude that the yield and quality of total RNA, microRNA, and DNA from a single PAXgene blood RNA tube is sufficient for downstream microarray analysis. |
| doi_str_mv | 10.2353/jmoldx.2007.060175 |
| format | Article |
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We conclude that the yield and quality of total RNA, microRNA, and DNA from a single PAXgene blood RNA tube is sufficient for downstream microarray analysis.</description><identifier>ISSN: 1525-1578</identifier><identifier>EISSN: 1943-7811</identifier><identifier>DOI: 10.2353/jmoldx.2007.060175</identifier><identifier>PMID: 17690207</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Blood Specimen Collection - methods ; DNA - isolation & purification ; Genome, Human - genetics ; Humans ; MicroRNAs - isolation & purification ; Oligonucleotide Array Sequence Analysis ; Pathology ; Polymorphism, Single Nucleotide - genetics ; Technical Advance ; Time Factors</subject><ispartof>The Journal of molecular diagnostics : JMD, 2007-09, Vol.9 (4), p.452-458</ispartof><rights>American Society for Investigative Pathology and Association for Molecular Pathology</rights><rights>2007 American Society for Investigative Pathology and Association for Molecular Pathology</rights><rights>Copyright © American Society for Investigative Pathology and the Association for Molecular Pathology 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c603t-c4258b088c19af5d428f5682e545758218e5b17008b4c7b1f11d09197663ef093</citedby><cites>FETCH-LOGICAL-c603t-c4258b088c19af5d428f5682e545758218e5b17008b4c7b1f11d09197663ef093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1525157810604163$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17690207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kruhøffer, Mogens</creatorcontrib><creatorcontrib>Dyrskjøt, Lars</creatorcontrib><creatorcontrib>Voss, Thorsten</creatorcontrib><creatorcontrib>Lindberg, Raija L.P</creatorcontrib><creatorcontrib>Wyrich, Ralf</creatorcontrib><creatorcontrib>Thykjaer, Thomas</creatorcontrib><creatorcontrib>Orntoft, Torben F</creatorcontrib><title>Isolation of Microarray-Grade Total RNA, MicroRNA, and DNA from a Single PAXgene Blood RNA Tube</title><title>The Journal of molecular diagnostics : JMD</title><addtitle>J Mol Diagn</addtitle><description>We have developed a procedure for isolation of microRNA and genomic DNA in addition to total RNA from whole blood stabilized in PAXgene Blood RNA tubes. 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We conclude that the yield and quality of total RNA, microRNA, and DNA from a single PAXgene blood RNA tube is sufficient for downstream microarray analysis.</description><subject>Blood Specimen Collection - methods</subject><subject>DNA - isolation & purification</subject><subject>Genome, Human - genetics</subject><subject>Humans</subject><subject>MicroRNAs - isolation & purification</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Pathology</subject><subject>Polymorphism, Single Nucleotide - genetics</subject><subject>Technical Advance</subject><subject>Time Factors</subject><issn>1525-1578</issn><issn>1943-7811</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kk1v1DAQhiMEoh_wBzggH1BPzeJx4jiRUKWlQKlUCqKLxM1ynMmuFycudlLYf49DVnwdOHkkP-_r8byTJE-ALljGs-fbztnm-4JRKha0oCD4veQQqjxLRQlwP9ac8RS4KA-SoxC2lEKeF-xhcgCiqCij4jCRl8FZNRjXE9eSd0Z7p7xXu_TCqwbJyg3Kko_Xy9P57mel-oa8ul6S1ruOKHJj-rVF8mH5eY09kpfWuWaSkNVY46PkQatswMf78zj59Ob16vxtevX-4vJ8eZXqgmZDqnPGy5qWpYZKtbzJWdnyomTIcy54yaBEXoOgtKxzLWpoARpaQSWKIsOWVtlxcjb73o51h43GfvDKyltvOuV30ikj_77pzUau3Z2MHpxWIhqc7A28-zpiGGRngkZrVY9uDDI2A7QAHkE2g3EcIXhsfz0CVE65yDkXOeUi51yi6Omf7f2W7IOIwLMZ2Jj15pvxKEOnrI04RL-mkrnMOYvYixnDOMs7g14GbbDX2ESJHmTjzP_bOPtHrq3pjVb2C-4wbN3o-5iSBBmYpPJmWqBpf-LPaQ5Flv0Aa8698Q</recordid><startdate>20070901</startdate><enddate>20070901</enddate><creator>Kruhøffer, Mogens</creator><creator>Dyrskjøt, Lars</creator><creator>Voss, Thorsten</creator><creator>Lindberg, Raija L.P</creator><creator>Wyrich, Ralf</creator><creator>Thykjaer, Thomas</creator><creator>Orntoft, Torben F</creator><general>Elsevier Inc</general><general>ASIP</general><general>American Society for Investigative Pathology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20070901</creationdate><title>Isolation of Microarray-Grade Total RNA, MicroRNA, and DNA from a Single PAXgene Blood RNA Tube</title><author>Kruhøffer, Mogens ; Dyrskjøt, Lars ; Voss, Thorsten ; Lindberg, Raija L.P ; Wyrich, Ralf ; Thykjaer, Thomas ; Orntoft, Torben F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c603t-c4258b088c19af5d428f5682e545758218e5b17008b4c7b1f11d09197663ef093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Blood Specimen Collection - methods</topic><topic>DNA - isolation & purification</topic><topic>Genome, Human - genetics</topic><topic>Humans</topic><topic>MicroRNAs - isolation & purification</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Pathology</topic><topic>Polymorphism, Single Nucleotide - genetics</topic><topic>Technical Advance</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kruhøffer, Mogens</creatorcontrib><creatorcontrib>Dyrskjøt, Lars</creatorcontrib><creatorcontrib>Voss, Thorsten</creatorcontrib><creatorcontrib>Lindberg, Raija L.P</creatorcontrib><creatorcontrib>Wyrich, Ralf</creatorcontrib><creatorcontrib>Thykjaer, Thomas</creatorcontrib><creatorcontrib>Orntoft, Torben F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of molecular diagnostics : JMD</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kruhøffer, Mogens</au><au>Dyrskjøt, Lars</au><au>Voss, Thorsten</au><au>Lindberg, Raija L.P</au><au>Wyrich, Ralf</au><au>Thykjaer, Thomas</au><au>Orntoft, Torben F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of Microarray-Grade Total RNA, MicroRNA, and DNA from a Single PAXgene Blood RNA Tube</atitle><jtitle>The Journal of molecular diagnostics : JMD</jtitle><addtitle>J Mol Diagn</addtitle><date>2007-09-01</date><risdate>2007</risdate><volume>9</volume><issue>4</issue><spage>452</spage><epage>458</epage><pages>452-458</pages><issn>1525-1578</issn><eissn>1943-7811</eissn><abstract>We have developed a procedure for isolation of microRNA and genomic DNA in addition to total RNA from whole blood stabilized in PAXgene Blood RNA tubes. 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| identifier | ISSN: 1525-1578 |
| ispartof | The Journal of molecular diagnostics : JMD, 2007-09, Vol.9 (4), p.452-458 |
| issn | 1525-1578 1943-7811 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Blood Specimen Collection - methods DNA - isolation & purification Genome, Human - genetics Humans MicroRNAs - isolation & purification Oligonucleotide Array Sequence Analysis Pathology Polymorphism, Single Nucleotide - genetics Technical Advance Time Factors |
| title | Isolation of Microarray-Grade Total RNA, MicroRNA, and DNA from a Single PAXgene Blood RNA Tube |
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