Sequence-independent DNA binding and replication initiation by the human origin recognition complex
We report that a highly purified human origin recognition complex (HsORC) has intrinsic DNA-binding activity, and that this activity is modestly stimulated by ATP. HsORC binds preferentially to synthetic AT-rich polydeoxynucleotides, but does not effectively discriminate between natural DNA fragment...
Gespeichert in:
| Veröffentlicht in: | Genes & development 2003-08, Vol.17 (15), p.1894-1908 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1908 |
|---|---|
| container_issue | 15 |
| container_start_page | 1894 |
| container_title | Genes & development |
| container_volume | 17 |
| creator | Vashee, Sanjay Cvetic, Christin Lu, Wenyan Simancek, Pamela Kelly, Thomas J Walter, Johannes C |
| description | We report that a highly purified human origin recognition complex (HsORC) has intrinsic DNA-binding activity, and that this activity is modestly stimulated by ATP. HsORC binds preferentially to synthetic AT-rich polydeoxynucleotides, but does not effectively discriminate between natural DNA fragments that contain known human origins and control fragments. The complex fully restores DNA replication to ORC-depleted Xenopus egg extracts, providing strong evidence for its initiator function. Strikingly, HsORC stimulates initiation from any DNA sequence, and it does not preferentially replicate DNA containing human origin sequences. These data provide a biochemical explanation for the observation that in metazoans, initiation of DNA replication often occurs in a seemingly random pattern, and they have important implications for the nature of human origins of DNA replication. |
| doi_str_mv | 10.1101/gad.1084203 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_196240</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>18902457</sourcerecordid><originalsourceid>FETCH-LOGICAL-c473t-95ed7e82b6cf9eda055cc5d9de781ec7af64894f94ca7d6201f0286023b3f49a3</originalsourceid><addsrcrecordid>eNqFkUtPxCAUhYnROONj5d505cZ0BEqhLFyY8ZkYXahrQuG2g2mhth2j_14mM_GxcsMl3O-Sc89B6IjgGSGYnNXazgguGMXZFpqSnMk0Z0JsoykuJE5lxuUE7Q3DK8aYY8530YTQQgqc51NknuBtCd5A6ryFDuLhx-Ty4SIp44PzdaK9TXroGmf06IJPnHejW1_Lz2RcQLJYttonoXe18xE1oV4hsW9C2zXwcYB2Kt0McLip--jl-up5fpveP97czS_uU8NENqYyByugoCU3lQSroz5jcistiIKAEbrirJCsksxoYTnFpMK04JhmZVYxqbN9dL7-t1uWLVgTN-l1o7retbr_VEE79bfj3ULV4V0RySnDcf5kM9-HaMowqtYNBppGewjLQYkspyI6-y9Iou-U5SvwdA2aPgxDD9W3GILVKjwVw1Ob8CJ9_Fv_D7tJK_sCDoWYGg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18902457</pqid></control><display><type>article</type><title>Sequence-independent DNA binding and replication initiation by the human origin recognition complex</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Vashee, Sanjay ; Cvetic, Christin ; Lu, Wenyan ; Simancek, Pamela ; Kelly, Thomas J ; Walter, Johannes C</creator><creatorcontrib>Vashee, Sanjay ; Cvetic, Christin ; Lu, Wenyan ; Simancek, Pamela ; Kelly, Thomas J ; Walter, Johannes C</creatorcontrib><description>We report that a highly purified human origin recognition complex (HsORC) has intrinsic DNA-binding activity, and that this activity is modestly stimulated by ATP. HsORC binds preferentially to synthetic AT-rich polydeoxynucleotides, but does not effectively discriminate between natural DNA fragments that contain known human origins and control fragments. The complex fully restores DNA replication to ORC-depleted Xenopus egg extracts, providing strong evidence for its initiator function. Strikingly, HsORC stimulates initiation from any DNA sequence, and it does not preferentially replicate DNA containing human origin sequences. These data provide a biochemical explanation for the observation that in metazoans, initiation of DNA replication often occurs in a seemingly random pattern, and they have important implications for the nature of human origins of DNA replication.</description><identifier>ISSN: 0890-9369</identifier><identifier>EISSN: 1549-5477</identifier><identifier>DOI: 10.1101/gad.1084203</identifier><identifier>PMID: 12897055</identifier><language>eng</language><publisher>United States: Cold Spring Harbor Laboratory Press</publisher><subject>Animals ; Binding, Competitive ; Cell Line ; Cell-Free System ; DNA - metabolism ; DNA Replication ; DNA-Binding Proteins ; Dose-Response Relationship, Drug ; Humans ; Insecta ; Origin Recognition Complex ; Plasmids - metabolism ; Protein Binding ; Recombinant Proteins - metabolism ; Research Papers ; Time Factors ; Xenopus</subject><ispartof>Genes & development, 2003-08, Vol.17 (15), p.1894-1908</ispartof><rights>Copyright © 2003, Cold Spring Harbor Laboratory Press 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-95ed7e82b6cf9eda055cc5d9de781ec7af64894f94ca7d6201f0286023b3f49a3</citedby><cites>FETCH-LOGICAL-c473t-95ed7e82b6cf9eda055cc5d9de781ec7af64894f94ca7d6201f0286023b3f49a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC196240/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC196240/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12897055$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vashee, Sanjay</creatorcontrib><creatorcontrib>Cvetic, Christin</creatorcontrib><creatorcontrib>Lu, Wenyan</creatorcontrib><creatorcontrib>Simancek, Pamela</creatorcontrib><creatorcontrib>Kelly, Thomas J</creatorcontrib><creatorcontrib>Walter, Johannes C</creatorcontrib><title>Sequence-independent DNA binding and replication initiation by the human origin recognition complex</title><title>Genes & development</title><addtitle>Genes Dev</addtitle><description>We report that a highly purified human origin recognition complex (HsORC) has intrinsic DNA-binding activity, and that this activity is modestly stimulated by ATP. HsORC binds preferentially to synthetic AT-rich polydeoxynucleotides, but does not effectively discriminate between natural DNA fragments that contain known human origins and control fragments. The complex fully restores DNA replication to ORC-depleted Xenopus egg extracts, providing strong evidence for its initiator function. Strikingly, HsORC stimulates initiation from any DNA sequence, and it does not preferentially replicate DNA containing human origin sequences. These data provide a biochemical explanation for the observation that in metazoans, initiation of DNA replication often occurs in a seemingly random pattern, and they have important implications for the nature of human origins of DNA replication.</description><subject>Animals</subject><subject>Binding, Competitive</subject><subject>Cell Line</subject><subject>Cell-Free System</subject><subject>DNA - metabolism</subject><subject>DNA Replication</subject><subject>DNA-Binding Proteins</subject><subject>Dose-Response Relationship, Drug</subject><subject>Humans</subject><subject>Insecta</subject><subject>Origin Recognition Complex</subject><subject>Plasmids - metabolism</subject><subject>Protein Binding</subject><subject>Recombinant Proteins - metabolism</subject><subject>Research Papers</subject><subject>Time Factors</subject><subject>Xenopus</subject><issn>0890-9369</issn><issn>1549-5477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtPxCAUhYnROONj5d505cZ0BEqhLFyY8ZkYXahrQuG2g2mhth2j_14mM_GxcsMl3O-Sc89B6IjgGSGYnNXazgguGMXZFpqSnMk0Z0JsoykuJE5lxuUE7Q3DK8aYY8530YTQQgqc51NknuBtCd5A6ryFDuLhx-Ty4SIp44PzdaK9TXroGmf06IJPnHejW1_Lz2RcQLJYttonoXe18xE1oV4hsW9C2zXwcYB2Kt0McLip--jl-up5fpveP97czS_uU8NENqYyByugoCU3lQSroz5jcistiIKAEbrirJCsksxoYTnFpMK04JhmZVYxqbN9dL7-t1uWLVgTN-l1o7retbr_VEE79bfj3ULV4V0RySnDcf5kM9-HaMowqtYNBppGewjLQYkspyI6-y9Iou-U5SvwdA2aPgxDD9W3GILVKjwVw1Ob8CJ9_Fv_D7tJK_sCDoWYGg</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>Vashee, Sanjay</creator><creator>Cvetic, Christin</creator><creator>Lu, Wenyan</creator><creator>Simancek, Pamela</creator><creator>Kelly, Thomas J</creator><creator>Walter, Johannes C</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20030801</creationdate><title>Sequence-independent DNA binding and replication initiation by the human origin recognition complex</title><author>Vashee, Sanjay ; Cvetic, Christin ; Lu, Wenyan ; Simancek, Pamela ; Kelly, Thomas J ; Walter, Johannes C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-95ed7e82b6cf9eda055cc5d9de781ec7af64894f94ca7d6201f0286023b3f49a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Binding, Competitive</topic><topic>Cell Line</topic><topic>Cell-Free System</topic><topic>DNA - metabolism</topic><topic>DNA Replication</topic><topic>DNA-Binding Proteins</topic><topic>Dose-Response Relationship, Drug</topic><topic>Humans</topic><topic>Insecta</topic><topic>Origin Recognition Complex</topic><topic>Plasmids - metabolism</topic><topic>Protein Binding</topic><topic>Recombinant Proteins - metabolism</topic><topic>Research Papers</topic><topic>Time Factors</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vashee, Sanjay</creatorcontrib><creatorcontrib>Cvetic, Christin</creatorcontrib><creatorcontrib>Lu, Wenyan</creatorcontrib><creatorcontrib>Simancek, Pamela</creatorcontrib><creatorcontrib>Kelly, Thomas J</creatorcontrib><creatorcontrib>Walter, Johannes C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genes & development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vashee, Sanjay</au><au>Cvetic, Christin</au><au>Lu, Wenyan</au><au>Simancek, Pamela</au><au>Kelly, Thomas J</au><au>Walter, Johannes C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence-independent DNA binding and replication initiation by the human origin recognition complex</atitle><jtitle>Genes & development</jtitle><addtitle>Genes Dev</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>17</volume><issue>15</issue><spage>1894</spage><epage>1908</epage><pages>1894-1908</pages><issn>0890-9369</issn><eissn>1549-5477</eissn><abstract>We report that a highly purified human origin recognition complex (HsORC) has intrinsic DNA-binding activity, and that this activity is modestly stimulated by ATP. HsORC binds preferentially to synthetic AT-rich polydeoxynucleotides, but does not effectively discriminate between natural DNA fragments that contain known human origins and control fragments. The complex fully restores DNA replication to ORC-depleted Xenopus egg extracts, providing strong evidence for its initiator function. Strikingly, HsORC stimulates initiation from any DNA sequence, and it does not preferentially replicate DNA containing human origin sequences. These data provide a biochemical explanation for the observation that in metazoans, initiation of DNA replication often occurs in a seemingly random pattern, and they have important implications for the nature of human origins of DNA replication.</abstract><cop>United States</cop><pub>Cold Spring Harbor Laboratory Press</pub><pmid>12897055</pmid><doi>10.1101/gad.1084203</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0890-9369 |
| ispartof | Genes & development, 2003-08, Vol.17 (15), p.1894-1908 |
| issn | 0890-9369 1549-5477 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_196240 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Binding, Competitive Cell Line Cell-Free System DNA - metabolism DNA Replication DNA-Binding Proteins Dose-Response Relationship, Drug Humans Insecta Origin Recognition Complex Plasmids - metabolism Protein Binding Recombinant Proteins - metabolism Research Papers Time Factors Xenopus |
| title | Sequence-independent DNA binding and replication initiation by the human origin recognition complex |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T07%3A24%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sequence-independent%20DNA%20binding%20and%20replication%20initiation%20by%20the%20human%20origin%20recognition%20complex&rft.jtitle=Genes%20&%20development&rft.au=Vashee,%20Sanjay&rft.date=2003-08-01&rft.volume=17&rft.issue=15&rft.spage=1894&rft.epage=1908&rft.pages=1894-1908&rft.issn=0890-9369&rft.eissn=1549-5477&rft_id=info:doi/10.1101/gad.1084203&rft_dat=%3Cproquest_pubme%3E18902457%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18902457&rft_id=info:pmid/12897055&rfr_iscdi=true |