Intrarenal cytokine gene expression in lupus nephritis
Background: Lupus nephritis is characterised by intrarenal inflammation and lymphocyte activation. Aim: To examine the profile of cytokine gene expression in glomerulus and tubulointerstitium in patients with lupus nephritis. Methods: 36 consecutive patients with systemic lupus erythematosus having...
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| Veröffentlicht in: | Annals of the rheumatic diseases 2007-07, Vol.66 (7), p.886-892 |
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| creator | Chan, Rebecca Wing-Yan Lai, Fernand Mac-Moune Li, Edmund Kwok-Ming Tam, Lai-Shan Chow, Kai-Ming Lai, Ka-Bik Li, Philip Kam-Tao Szeto, Cheuk-Chun |
| description | Background: Lupus nephritis is characterised by intrarenal inflammation and lymphocyte activation. Aim: To examine the profile of cytokine gene expression in glomerulus and tubulointerstitium in patients with lupus nephritis. Methods: 36 consecutive patients with systemic lupus erythematosus having active renal disease were recruited, and they were required to undergo kidney biopsy. Glomerular and tubulointestitial cytokine expression of interleukin (IL)2, 4, 10, 12, 18, interferon γ (IFN)γ, T-bet (the Th1 transcription factor), GATA-3 (the Th2 transcription factor), transforming growth factorβ and monocyte chemoattractant protein (MCP)1 were studied by laser microdissection of the renal biopsy specimen, followed by real-time quantitative PCR. Results: There were 13 patients with World Health Organization class III nephritis, 14 patients with class IV nephritis and 9 patients with class V nephritis. There was a significant correlation between serum C3, C4 and anti-double strand DNA antibody level with glomerular expression of T-bet, IFNγ and IL2. There was a significant correlation between histological activity index and glomerular expression of IL12, IL18, IL10 and MCP1. In addition, the degree of glomerular leucocyte infiltration significantly correlated with glomerular expression of IFNγ, IL10, IL12 and IL18. By contrast, histological chronicity index correlated with the tubulointerstitial expression of IL2, MCP1 and GATA-3. Conclusions: Intraglomerular expression of certain target genes correlate with the severity of systemic as well as histological activity, whereas the tubulointerstitial expression of other target genes correlate with the degree of chronic kidney scarring. This result may shed light on the immunopathogenesis of lupus nephritis. |
| doi_str_mv | 10.1136/ard.2006.063123 |
| format | Article |
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Aim: To examine the profile of cytokine gene expression in glomerulus and tubulointerstitium in patients with lupus nephritis. Methods: 36 consecutive patients with systemic lupus erythematosus having active renal disease were recruited, and they were required to undergo kidney biopsy. Glomerular and tubulointestitial cytokine expression of interleukin (IL)2, 4, 10, 12, 18, interferon γ (IFN)γ, T-bet (the Th1 transcription factor), GATA-3 (the Th2 transcription factor), transforming growth factorβ and monocyte chemoattractant protein (MCP)1 were studied by laser microdissection of the renal biopsy specimen, followed by real-time quantitative PCR. Results: There were 13 patients with World Health Organization class III nephritis, 14 patients with class IV nephritis and 9 patients with class V nephritis. There was a significant correlation between serum C3, C4 and anti-double strand DNA antibody level with glomerular expression of T-bet, IFNγ and IL2. There was a significant correlation between histological activity index and glomerular expression of IL12, IL18, IL10 and MCP1. In addition, the degree of glomerular leucocyte infiltration significantly correlated with glomerular expression of IFNγ, IL10, IL12 and IL18. By contrast, histological chronicity index correlated with the tubulointerstitial expression of IL2, MCP1 and GATA-3. Conclusions: Intraglomerular expression of certain target genes correlate with the severity of systemic as well as histological activity, whereas the tubulointerstitial expression of other target genes correlate with the degree of chronic kidney scarring. This result may shed light on the immunopathogenesis of lupus nephritis.</description><identifier>ISSN: 0003-4967</identifier><identifier>EISSN: 1468-2060</identifier><identifier>DOI: 10.1136/ard.2006.063123</identifier><identifier>PMID: 17324967</identifier><identifier>CODEN: ARDIAO</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and European League Against Rheumatism</publisher><subject>activity index ; Adult ; Biological and medical sciences ; Chemokine CCL2 - analysis ; Chemokine CCL2 - urine ; chronicity index ; Cytokines ; Cytokines - analysis ; Cytokines - urine ; Deoxyribonucleic acid ; DNA ; double strand ; Extended Report ; Female ; GATA3 Transcription Factor - analysis ; GATA3 Transcription Factor - urine ; Gene expression ; Gene Expression - genetics ; Gene Expression - immunology ; Genetic engineering ; Humans ; IFN ; interferon ; Interferon-gamma - analysis ; Interferon-gamma - urine ; interleukin ; Interleukins - analysis ; Interleukins - urine ; Kidney - immunology ; Kidney - pathology ; Kidney diseases ; Kidney Glomerulus - immunology ; Kidney Glomerulus - pathology ; Kidney Tubules - immunology ; Kidney Tubules - pathology ; Kidneys ; Lasers ; Leukocytes - immunology ; Lupus ; Lupus Nephritis - genetics ; Lupus Nephritis - immunology ; Lupus Nephritis - pathology ; Male ; MCP ; Medical sciences ; messenger RNA ; Methods ; monocyte chemoattractant protein ; mRNA ; Nephrology. Urinary tract diseases ; Polyethylene ; real-time quantitative PCR ; ribosomal RNA ; Rodents ; rRNA ; RT-QPCR ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis ; SLE ; SLEDAI ; Studies ; systemic lupus erythematosus ; Systemic Lupus Erythematosus Disease Activity Index ; T-Box Domain Proteins - analysis ; T-Box Domain Proteins - urine ; Transcription factors ; Transforming Growth Factor beta - analysis ; Transforming Growth Factor beta - urine ; Urinary system involvement in other diseases. Miscellaneous ; WHO ; World Health Organization</subject><ispartof>Annals of the rheumatic diseases, 2007-07, Vol.66 (7), p.886-892</ispartof><rights>Copyright 2007 by Annals of the Rheumatic Diseases</rights><rights>2007 INIST-CNRS</rights><rights>Copyright: 2007 Copyright 2007 by Annals of the Rheumatic Diseases</rights><rights>Copyright © 2007 BMJ Publishing Group and European League Against Rheumatism</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b553t-2f504b82ae1ee2c0ae538dcb2218c6843410ebfed91071685cf1d735f2a40ad93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttp://ard.bmj.com/content/66/7/886.full.pdf$$EPDF$$P50$$Gbmj$$H</linktopdf><linktohtml>$$Uhttp://ard.bmj.com/content/66/7/886.full$$EHTML$$P50$$Gbmj$$H</linktohtml><link.rule.ids>114,115,230,315,728,781,785,886,3197,23576,27929,27930,53796,53798,77605,77636</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18825256$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17324967$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chan, Rebecca Wing-Yan</creatorcontrib><creatorcontrib>Lai, Fernand Mac-Moune</creatorcontrib><creatorcontrib>Li, Edmund Kwok-Ming</creatorcontrib><creatorcontrib>Tam, Lai-Shan</creatorcontrib><creatorcontrib>Chow, Kai-Ming</creatorcontrib><creatorcontrib>Lai, Ka-Bik</creatorcontrib><creatorcontrib>Li, Philip Kam-Tao</creatorcontrib><creatorcontrib>Szeto, Cheuk-Chun</creatorcontrib><title>Intrarenal cytokine gene expression in lupus nephritis</title><title>Annals of the rheumatic diseases</title><addtitle>Ann Rheum Dis</addtitle><description>Background: Lupus nephritis is characterised by intrarenal inflammation and lymphocyte activation. Aim: To examine the profile of cytokine gene expression in glomerulus and tubulointerstitium in patients with lupus nephritis. Methods: 36 consecutive patients with systemic lupus erythematosus having active renal disease were recruited, and they were required to undergo kidney biopsy. Glomerular and tubulointestitial cytokine expression of interleukin (IL)2, 4, 10, 12, 18, interferon γ (IFN)γ, T-bet (the Th1 transcription factor), GATA-3 (the Th2 transcription factor), transforming growth factorβ and monocyte chemoattractant protein (MCP)1 were studied by laser microdissection of the renal biopsy specimen, followed by real-time quantitative PCR. Results: There were 13 patients with World Health Organization class III nephritis, 14 patients with class IV nephritis and 9 patients with class V nephritis. There was a significant correlation between serum C3, C4 and anti-double strand DNA antibody level with glomerular expression of T-bet, IFNγ and IL2. There was a significant correlation between histological activity index and glomerular expression of IL12, IL18, IL10 and MCP1. In addition, the degree of glomerular leucocyte infiltration significantly correlated with glomerular expression of IFNγ, IL10, IL12 and IL18. By contrast, histological chronicity index correlated with the tubulointerstitial expression of IL2, MCP1 and GATA-3. Conclusions: Intraglomerular expression of certain target genes correlate with the severity of systemic as well as histological activity, whereas the tubulointerstitial expression of other target genes correlate with the degree of chronic kidney scarring. This result may shed light on the immunopathogenesis of lupus nephritis.</description><subject>activity index</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Chemokine CCL2 - analysis</subject><subject>Chemokine CCL2 - urine</subject><subject>chronicity index</subject><subject>Cytokines</subject><subject>Cytokines - analysis</subject><subject>Cytokines - urine</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>double strand</subject><subject>Extended Report</subject><subject>Female</subject><subject>GATA3 Transcription Factor - analysis</subject><subject>GATA3 Transcription Factor - urine</subject><subject>Gene expression</subject><subject>Gene Expression - genetics</subject><subject>Gene Expression - immunology</subject><subject>Genetic engineering</subject><subject>Humans</subject><subject>IFN</subject><subject>interferon</subject><subject>Interferon-gamma - analysis</subject><subject>Interferon-gamma - urine</subject><subject>interleukin</subject><subject>Interleukins - analysis</subject><subject>Interleukins - urine</subject><subject>Kidney - immunology</subject><subject>Kidney - pathology</subject><subject>Kidney diseases</subject><subject>Kidney Glomerulus - immunology</subject><subject>Kidney Glomerulus - pathology</subject><subject>Kidney Tubules - immunology</subject><subject>Kidney Tubules - pathology</subject><subject>Kidneys</subject><subject>Lasers</subject><subject>Leukocytes - immunology</subject><subject>Lupus</subject><subject>Lupus Nephritis - genetics</subject><subject>Lupus Nephritis - immunology</subject><subject>Lupus Nephritis - pathology</subject><subject>Male</subject><subject>MCP</subject><subject>Medical sciences</subject><subject>messenger RNA</subject><subject>Methods</subject><subject>monocyte chemoattractant protein</subject><subject>mRNA</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Polyethylene</subject><subject>real-time quantitative PCR</subject><subject>ribosomal RNA</subject><subject>Rodents</subject><subject>rRNA</subject><subject>RT-QPCR</subject><subject>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</subject><subject>SLE</subject><subject>SLEDAI</subject><subject>Studies</subject><subject>systemic lupus erythematosus</subject><subject>Systemic Lupus Erythematosus Disease Activity Index</subject><subject>T-Box Domain Proteins - analysis</subject><subject>T-Box Domain Proteins - urine</subject><subject>Transcription factors</subject><subject>Transforming Growth Factor beta - analysis</subject><subject>Transforming Growth Factor beta - urine</subject><subject>Urinary system involvement in other diseases. Miscellaneous</subject><subject>WHO</subject><subject>World Health Organization</subject><issn>0003-4967</issn><issn>1468-2060</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0b9v1DAUB3ALgehRmNlQJESHSrk-2_GPLEjoVGilU2EoDCyW47y0vuaS1E5Q-9_jU04tdOliy_bHT8_-EvKewpJSLk9sqJcMQC5Bcsr4C7KghdQ5AwkvyQIAeF6UUh2QNzFu0hI01a_JAVWc7fYXRJ53Y7ABO9tm7n7sb3yH2RWmAe-GgDH6vst8l7XTMMWsw-E6-NHHt-RVY9uI7_bzIfn59fRydZavv387X31Z55UQfMxZI6CoNLNIEZkDi4Lr2lWMUe2kLnhBAasG65KColIL19BacdEwW4CtS35IPs91h6naYu1w121rhuC3Ntyb3nrz_0nnr81V_8fQUgjKWCpwtC8Q-tsJ42i2PjpsW9thP0WjQCYqxbOQlloKISHBj0_gpp9C-r9klFJaUKl4UiezcqGPMWDz0DMFs4vOpOjMLjozR5dufPj3qY9-n1UCn_bARmfbJtjO-fjotGaCCZlcPjsfR7x7OLfhxqQqSpiLX6v0nEtRyt8_zDr549lX282zXf4FsmS9cw</recordid><startdate>20070701</startdate><enddate>20070701</enddate><creator>Chan, Rebecca Wing-Yan</creator><creator>Lai, Fernand Mac-Moune</creator><creator>Li, Edmund Kwok-Ming</creator><creator>Tam, Lai-Shan</creator><creator>Chow, Kai-Ming</creator><creator>Lai, Ka-Bik</creator><creator>Li, Philip Kam-Tao</creator><creator>Szeto, Cheuk-Chun</creator><general>BMJ Publishing Group Ltd and European League Against Rheumatism</general><general>BMJ</general><general>BMJ Publishing Group LTD</general><general>BMJ Group</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20070701</creationdate><title>Intrarenal cytokine gene expression in lupus nephritis</title><author>Chan, Rebecca Wing-Yan ; Lai, Fernand Mac-Moune ; Li, Edmund Kwok-Ming ; Tam, Lai-Shan ; Chow, Kai-Ming ; Lai, Ka-Bik ; Li, Philip Kam-Tao ; Szeto, Cheuk-Chun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b553t-2f504b82ae1ee2c0ae538dcb2218c6843410ebfed91071685cf1d735f2a40ad93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>activity index</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Chemokine CCL2 - analysis</topic><topic>Chemokine CCL2 - urine</topic><topic>chronicity index</topic><topic>Cytokines</topic><topic>Cytokines - analysis</topic><topic>Cytokines - urine</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>double strand</topic><topic>Extended Report</topic><topic>Female</topic><topic>GATA3 Transcription Factor - analysis</topic><topic>GATA3 Transcription Factor - urine</topic><topic>Gene expression</topic><topic>Gene Expression - genetics</topic><topic>Gene Expression - immunology</topic><topic>Genetic engineering</topic><topic>Humans</topic><topic>IFN</topic><topic>interferon</topic><topic>Interferon-gamma - analysis</topic><topic>Interferon-gamma - urine</topic><topic>interleukin</topic><topic>Interleukins - analysis</topic><topic>Interleukins - urine</topic><topic>Kidney - immunology</topic><topic>Kidney - pathology</topic><topic>Kidney diseases</topic><topic>Kidney Glomerulus - immunology</topic><topic>Kidney Glomerulus - pathology</topic><topic>Kidney Tubules - immunology</topic><topic>Kidney Tubules - pathology</topic><topic>Kidneys</topic><topic>Lasers</topic><topic>Leukocytes - immunology</topic><topic>Lupus</topic><topic>Lupus Nephritis - genetics</topic><topic>Lupus Nephritis - immunology</topic><topic>Lupus Nephritis - pathology</topic><topic>Male</topic><topic>MCP</topic><topic>Medical sciences</topic><topic>messenger RNA</topic><topic>Methods</topic><topic>monocyte chemoattractant protein</topic><topic>mRNA</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Polyethylene</topic><topic>real-time quantitative PCR</topic><topic>ribosomal RNA</topic><topic>Rodents</topic><topic>rRNA</topic><topic>RT-QPCR</topic><topic>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</topic><topic>SLE</topic><topic>SLEDAI</topic><topic>Studies</topic><topic>systemic lupus erythematosus</topic><topic>Systemic Lupus Erythematosus Disease Activity Index</topic><topic>T-Box Domain Proteins - analysis</topic><topic>T-Box Domain Proteins - urine</topic><topic>Transcription factors</topic><topic>Transforming Growth Factor beta - analysis</topic><topic>Transforming Growth Factor beta - urine</topic><topic>Urinary system involvement in other diseases. Miscellaneous</topic><topic>WHO</topic><topic>World Health Organization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chan, Rebecca Wing-Yan</creatorcontrib><creatorcontrib>Lai, Fernand Mac-Moune</creatorcontrib><creatorcontrib>Li, Edmund Kwok-Ming</creatorcontrib><creatorcontrib>Tam, Lai-Shan</creatorcontrib><creatorcontrib>Chow, Kai-Ming</creatorcontrib><creatorcontrib>Lai, Ka-Bik</creatorcontrib><creatorcontrib>Li, Philip Kam-Tao</creatorcontrib><creatorcontrib>Szeto, Cheuk-Chun</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>ProQuest Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Annals of the rheumatic diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chan, Rebecca Wing-Yan</au><au>Lai, Fernand Mac-Moune</au><au>Li, Edmund Kwok-Ming</au><au>Tam, Lai-Shan</au><au>Chow, Kai-Ming</au><au>Lai, Ka-Bik</au><au>Li, Philip Kam-Tao</au><au>Szeto, Cheuk-Chun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intrarenal cytokine gene expression in lupus nephritis</atitle><jtitle>Annals of the rheumatic diseases</jtitle><addtitle>Ann Rheum Dis</addtitle><date>2007-07-01</date><risdate>2007</risdate><volume>66</volume><issue>7</issue><spage>886</spage><epage>892</epage><pages>886-892</pages><issn>0003-4967</issn><eissn>1468-2060</eissn><coden>ARDIAO</coden><abstract>Background: Lupus nephritis is characterised by intrarenal inflammation and lymphocyte activation. Aim: To examine the profile of cytokine gene expression in glomerulus and tubulointerstitium in patients with lupus nephritis. Methods: 36 consecutive patients with systemic lupus erythematosus having active renal disease were recruited, and they were required to undergo kidney biopsy. Glomerular and tubulointestitial cytokine expression of interleukin (IL)2, 4, 10, 12, 18, interferon γ (IFN)γ, T-bet (the Th1 transcription factor), GATA-3 (the Th2 transcription factor), transforming growth factorβ and monocyte chemoattractant protein (MCP)1 were studied by laser microdissection of the renal biopsy specimen, followed by real-time quantitative PCR. Results: There were 13 patients with World Health Organization class III nephritis, 14 patients with class IV nephritis and 9 patients with class V nephritis. There was a significant correlation between serum C3, C4 and anti-double strand DNA antibody level with glomerular expression of T-bet, IFNγ and IL2. There was a significant correlation between histological activity index and glomerular expression of IL12, IL18, IL10 and MCP1. In addition, the degree of glomerular leucocyte infiltration significantly correlated with glomerular expression of IFNγ, IL10, IL12 and IL18. By contrast, histological chronicity index correlated with the tubulointerstitial expression of IL2, MCP1 and GATA-3. Conclusions: Intraglomerular expression of certain target genes correlate with the severity of systemic as well as histological activity, whereas the tubulointerstitial expression of other target genes correlate with the degree of chronic kidney scarring. This result may shed light on the immunopathogenesis of lupus nephritis.</abstract><cop>London</cop><pub>BMJ Publishing Group Ltd and European League Against Rheumatism</pub><pmid>17324967</pmid><doi>10.1136/ard.2006.063123</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Annals of the rheumatic diseases, 2007-07, Vol.66 (7), p.886-892 |
| issn | 0003-4967 1468-2060 |
| language | eng |
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| subjects | activity index Adult Biological and medical sciences Chemokine CCL2 - analysis Chemokine CCL2 - urine chronicity index Cytokines Cytokines - analysis Cytokines - urine Deoxyribonucleic acid DNA double strand Extended Report Female GATA3 Transcription Factor - analysis GATA3 Transcription Factor - urine Gene expression Gene Expression - genetics Gene Expression - immunology Genetic engineering Humans IFN interferon Interferon-gamma - analysis Interferon-gamma - urine interleukin Interleukins - analysis Interleukins - urine Kidney - immunology Kidney - pathology Kidney diseases Kidney Glomerulus - immunology Kidney Glomerulus - pathology Kidney Tubules - immunology Kidney Tubules - pathology Kidneys Lasers Leukocytes - immunology Lupus Lupus Nephritis - genetics Lupus Nephritis - immunology Lupus Nephritis - pathology Male MCP Medical sciences messenger RNA Methods monocyte chemoattractant protein mRNA Nephrology. Urinary tract diseases Polyethylene real-time quantitative PCR ribosomal RNA Rodents rRNA RT-QPCR Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis SLE SLEDAI Studies systemic lupus erythematosus Systemic Lupus Erythematosus Disease Activity Index T-Box Domain Proteins - analysis T-Box Domain Proteins - urine Transcription factors Transforming Growth Factor beta - analysis Transforming Growth Factor beta - urine Urinary system involvement in other diseases. Miscellaneous WHO World Health Organization |
| title | Intrarenal cytokine gene expression in lupus nephritis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T18%3A00%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Intrarenal%20cytokine%20gene%20expression%20in%20lupus%20nephritis&rft.jtitle=Annals%20of%20the%20rheumatic%20diseases&rft.au=Chan,%20Rebecca%20Wing-Yan&rft.date=2007-07-01&rft.volume=66&rft.issue=7&rft.spage=886&rft.epage=892&rft.pages=886-892&rft.issn=0003-4967&rft.eissn=1468-2060&rft.coden=ARDIAO&rft_id=info:doi/10.1136/ard.2006.063123&rft_dat=%3Cproquest_pubme%3E4008407731%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1777851673&rft_id=info:pmid/17324967&rfr_iscdi=true |