Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I

Crystals of site‐specific DNA nickase Nb.BspD6I (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 Å resolution at 100 K. The crystal belongs to space group P21, with unit‐cell parameters a = 57.76, b = 90.67,...

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Veröffentlicht in:	Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2005-03, Vol.61 (3), p.332-334
Hauptverfasser:	Kachalova, Galina S., Rogulin, Eugeny A., Artyukh, Rimma I., Perevyazova, Tatyana A., Zheleznaya, Ludmila A., Matvienko, Nickolay I., Bartunik, Hans D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus - enzymology Bacillus sp. D6 Bacterial Proteins - chemistry Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Crystallization Crystallization Communications Crystallography, X-Ray Deoxyribonuclease I - chemistry Deoxyribonuclease I - isolation & purification Deoxyribonuclease I - metabolism Kinetics Nb.BspD6I site-specific DNA nickase Substrate Specificity X-Ray Diffraction
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