Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I
Crystals of site‐specific DNA nickase Nb.BspD6I (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 Å resolution at 100 K. The crystal belongs to space group P21, with unit‐cell parameters a = 57.76, b = 90.67,...
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| Veröffentlicht in: | Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2005-03, Vol.61 (3), p.332-334 |
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| container_title | Acta crystallographica. Section F, Structural biology and crystallization communications |
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| creator | Kachalova, Galina S. Rogulin, Eugeny A. Artyukh, Rimma I. Perevyazova, Tatyana A. Zheleznaya, Ludmila A. Matvienko, Nickolay I. Bartunik, Hans D. |
| description | Crystals of site‐specific DNA nickase Nb.BspD6I (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 Å resolution at 100 K. The crystal belongs to space group P21, with unit‐cell parameters a = 57.76, b = 90.67, c = 71.71, β = 110.1°. There is one molecule in the asymmetric unit and the solvent content is estimated to be 53% by volume. |
| doi_str_mv | 10.1107/S174430910500309X |
| format | Article |
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The diffraction pattern of the crystal extends to 3.3 Å resolution at 100 K. The crystal belongs to space group P21, with unit‐cell parameters a = 57.76, b = 90.67, c = 71.71, β = 110.1°. There is one molecule in the asymmetric unit and the solvent content is estimated to be 53% by volume.</description><identifier>ISSN: 1744-3091</identifier><identifier>EISSN: 1744-3091</identifier><identifier>DOI: 10.1107/S174430910500309X</identifier><identifier>PMID: 16511033</identifier><language>eng</language><publisher>5 Abbey Square, Chester, Cheshire CH1 2HU, England: Munksgaard International Publishers</publisher><subject>Bacillus - enzymology ; Bacillus sp. 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Section F, Structural biology and crystallization communications</title><addtitle>Acta Cryst. F</addtitle><description>Crystals of site‐specific DNA nickase Nb.BspD6I (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 Å resolution at 100 K. The crystal belongs to space group P21, with unit‐cell parameters a = 57.76, b = 90.67, c = 71.71, β = 110.1°. There is one molecule in the asymmetric unit and the solvent content is estimated to be 53% by volume.</description><subject>Bacillus - enzymology</subject><subject>Bacillus sp. D6</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacterial Proteins - metabolism</subject><subject>Crystallization</subject><subject>Crystallization Communications</subject><subject>Crystallography, X-Ray</subject><subject>Deoxyribonuclease I - chemistry</subject><subject>Deoxyribonuclease I - isolation & purification</subject><subject>Deoxyribonuclease I - metabolism</subject><subject>Kinetics</subject><subject>Nb.BspD6I</subject><subject>site-specific DNA nickase</subject><subject>Substrate Specificity</subject><subject>X-Ray Diffraction</subject><issn>1744-3091</issn><issn>1744-3091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9P3DAQxS1UxP8P0EuVU28BTxzHzgVpdykLEiwgQNCT5dgO6-JNUjtLu_309WpXFMShp7Hm_d6bsQahz4APATA7ugWW5wSXgCnGsT5uoJ1lK132Pr15b6PdEH5EhpQF30LbUNAYQMgO0iO_CL10zv6RvW2bRDY66bxxdmYb6ReJWuvtk5fd1KoISLcINiRtnfRTkwTbmzR0Rtk6qieTQdJY9SyDSSbV4TB0J8X5PtqspQvmYF330P3pt7vRWXpxNT4fDS5SlXPK0rzEjBdaUw28rjNQKpc0JxqyghrGgFfAgauCQGUiooFRQlhe1VJTXmWU7KHjVW43r2ZGK9P0XjrReTuLXxGttOK90tipeGpfBJQ0yziOAV_XAb79OTehFzMblHFONqadB8EwEFpQFkFYgcq3IXhTvw4BLJa3ER9uEz1f3m73z7E-RgTKFfDLOrP4f6IYfD_Nhg8Us-VC6cprQ29-v3qlfxYFI4yKh8lYDG-uR0N8ORac_AUQMqqU</recordid><startdate>200503</startdate><enddate>200503</enddate><creator>Kachalova, Galina S.</creator><creator>Rogulin, Eugeny A.</creator><creator>Artyukh, Rimma I.</creator><creator>Perevyazova, Tatyana A.</creator><creator>Zheleznaya, Ludmila A.</creator><creator>Matvienko, Nickolay I.</creator><creator>Bartunik, Hans D.</creator><general>Munksgaard International Publishers</general><general>International Union of Crystallography</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200503</creationdate><title>Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I</title><author>Kachalova, Galina S. ; Rogulin, Eugeny A. ; Artyukh, Rimma I. ; Perevyazova, Tatyana A. ; Zheleznaya, Ludmila A. ; Matvienko, Nickolay I. ; Bartunik, Hans D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4857-490786dd5d18ff21cc4a543d1265e7718b1818c631bed18d1753374bfad58b253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Bacillus - enzymology</topic><topic>Bacillus sp. 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F</addtitle><date>2005-03</date><risdate>2005</risdate><volume>61</volume><issue>3</issue><spage>332</spage><epage>334</epage><pages>332-334</pages><issn>1744-3091</issn><eissn>1744-3091</eissn><abstract>Crystals of site‐specific DNA nickase Nb.BspD6I (of molecular weight 70.8 kDa) have been grown at 291 K using PEG 8000 as precipitant. The diffraction pattern of the crystal extends to 3.3 Å resolution at 100 K. The crystal belongs to space group P21, with unit‐cell parameters a = 57.76, b = 90.67, c = 71.71, β = 110.1°. There is one molecule in the asymmetric unit and the solvent content is estimated to be 53% by volume.</abstract><cop>5 Abbey Square, Chester, Cheshire CH1 2HU, England</cop><pub>Munksgaard International Publishers</pub><pmid>16511033</pmid><doi>10.1107/S174430910500309X</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Bacillus - enzymology Bacillus sp. D6 Bacterial Proteins - chemistry Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Crystallization Crystallization Communications Crystallography, X-Ray Deoxyribonuclease I - chemistry Deoxyribonuclease I - isolation & purification Deoxyribonuclease I - metabolism Kinetics Nb.BspD6I site-specific DNA nickase Substrate Specificity X-Ray Diffraction |
| title | Crystallization and preliminary crystallographic analysis of the site-specific DNA nickase Nb.BspD6I |
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