Distinct ribonucleoprotein reservoirs for microRNA and siRNA populations in C. elegans
MicroRNAs (miRNAs) are regulatory molecules that share both biosynthetic derivation (cleavage from short hairpin precursor RNAs) and functional roles (downregulation of specific mRNAs through targeted degradation and/or translational inhibition). A distinct family of small RNAs, termed siRNAs, have...
Gespeichert in:
| Veröffentlicht in: | RNA (Cambridge) 2007-09, Vol.13 (9), p.1492-1504 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1504 |
|---|---|
| container_issue | 9 |
| container_start_page | 1492 |
| container_title | RNA (Cambridge) |
| container_volume | 13 |
| creator | Gu, Sam G Pak, Julia Barberan-Soler, Sergio Ali, Mustapha Fire, Andrew Zahler, Alan M |
| description | MicroRNAs (miRNAs) are regulatory molecules that share both biosynthetic derivation (cleavage from short hairpin precursor RNAs) and functional roles (downregulation of specific mRNAs through targeted degradation and/or translational inhibition). A distinct family of small RNAs, termed siRNAs, have some common characteristics but exhibit distinct modes of biosynthesis and function. In this study, we report procedures for purification of a predominant species of miRNA-containing ribonucleoprotein complexes from Caenorhabditis elegans and demonstrate that this population is distinct from the predominant pool of siRNA-containing ribonucleoprotein complexes. An observed miRNP-associated RNA population consisting predominantly (>95%) of miRNAs supported the unique identity of miRNPs as biological effectors within the cell, provided clean material for analysis of changes in miRNA spectra during development, and provided strong evidence of miRNA character for a number of novel small RNAs. Likewise, the RNA spectrum derived from partial siRNP purification was useful in defining functional characteristics of this more diverse population of small RNAs. |
| doi_str_mv | 10.1261/rna.581907 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1950768</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20136569</sourcerecordid><originalsourceid>FETCH-LOGICAL-c376t-b51d163c3f728a40660d57dba41d57a54deec3de35a3b3a04da577e9b4f073dc3</originalsourceid><addsrcrecordid>eNpVUU1LxDAQDaK4fl38AdKTB6Fr0jRJexGW9RNEQdRrSJOpRrpJTdoF_71ZdvHjNI-ZN2-G9xA6JnhKCk7Og1NTVpEaiy20R0pe5zXGZDthylhe0aqYoP0YP1KTpvEumhDBWUFotYdeL20crNNDFmzj3ag78H3wA1iXBYgQlt6GmLU-ZAurg396mGXKmSzaFep9P3ZqsN7FLC3Mpxl08KZcPEQ7reoiHG3qAXq5vnqe3-b3jzd389l9rqngQ94wYginmraiqFSJOceGCdOokqSqWGkANDVAmaINVbg0igkBdVO2WFCj6QG6WOv2Y7MAo8ENQXWyD3ahwpf0ysr_E2ff5ZtfSlIzLHiVBE43AsF_jhAHubBRQ9cpB36MskieccbrRDxbE5MJMQZof44QLFcxyBSDXMeQyCd_3_qlbnyn39g3hYE</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20136569</pqid></control><display><type>article</type><title>Distinct ribonucleoprotein reservoirs for microRNA and siRNA populations in C. elegans</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Gu, Sam G ; Pak, Julia ; Barberan-Soler, Sergio ; Ali, Mustapha ; Fire, Andrew ; Zahler, Alan M</creator><creatorcontrib>Gu, Sam G ; Pak, Julia ; Barberan-Soler, Sergio ; Ali, Mustapha ; Fire, Andrew ; Zahler, Alan M</creatorcontrib><description>MicroRNAs (miRNAs) are regulatory molecules that share both biosynthetic derivation (cleavage from short hairpin precursor RNAs) and functional roles (downregulation of specific mRNAs through targeted degradation and/or translational inhibition). A distinct family of small RNAs, termed siRNAs, have some common characteristics but exhibit distinct modes of biosynthesis and function. In this study, we report procedures for purification of a predominant species of miRNA-containing ribonucleoprotein complexes from Caenorhabditis elegans and demonstrate that this population is distinct from the predominant pool of siRNA-containing ribonucleoprotein complexes. An observed miRNP-associated RNA population consisting predominantly (>95%) of miRNAs supported the unique identity of miRNPs as biological effectors within the cell, provided clean material for analysis of changes in miRNA spectra during development, and provided strong evidence of miRNA character for a number of novel small RNAs. Likewise, the RNA spectrum derived from partial siRNP purification was useful in defining functional characteristics of this more diverse population of small RNAs.</description><identifier>ISSN: 1355-8382</identifier><identifier>EISSN: 1469-9001</identifier><identifier>DOI: 10.1261/rna.581907</identifier><identifier>PMID: 17652138</identifier><language>eng</language><publisher>United States: Cold Spring Harbor Laboratory Press</publisher><subject>Animals ; Caenorhabditis elegans ; Caenorhabditis elegans - genetics ; Caenorhabditis elegans - metabolism ; Caenorhabditis elegans Proteins - genetics ; Caenorhabditis elegans Proteins - metabolism ; MicroRNAs - analysis ; MicroRNAs - isolation & purification ; MicroRNAs - metabolism ; Ribonucleoproteins - metabolism ; RNA, Small Interfering - analysis ; RNA, Small Interfering - isolation & purification ; RNA, Small Interfering - metabolism</subject><ispartof>RNA (Cambridge), 2007-09, Vol.13 (9), p.1492-1504</ispartof><rights>Copyright © 2007 RNA Society 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-b51d163c3f728a40660d57dba41d57a54deec3de35a3b3a04da577e9b4f073dc3</citedby><cites>FETCH-LOGICAL-c376t-b51d163c3f728a40660d57dba41d57a54deec3de35a3b3a04da577e9b4f073dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1950768/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1950768/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17652138$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gu, Sam G</creatorcontrib><creatorcontrib>Pak, Julia</creatorcontrib><creatorcontrib>Barberan-Soler, Sergio</creatorcontrib><creatorcontrib>Ali, Mustapha</creatorcontrib><creatorcontrib>Fire, Andrew</creatorcontrib><creatorcontrib>Zahler, Alan M</creatorcontrib><title>Distinct ribonucleoprotein reservoirs for microRNA and siRNA populations in C. elegans</title><title>RNA (Cambridge)</title><addtitle>RNA</addtitle><description>MicroRNAs (miRNAs) are regulatory molecules that share both biosynthetic derivation (cleavage from short hairpin precursor RNAs) and functional roles (downregulation of specific mRNAs through targeted degradation and/or translational inhibition). A distinct family of small RNAs, termed siRNAs, have some common characteristics but exhibit distinct modes of biosynthesis and function. In this study, we report procedures for purification of a predominant species of miRNA-containing ribonucleoprotein complexes from Caenorhabditis elegans and demonstrate that this population is distinct from the predominant pool of siRNA-containing ribonucleoprotein complexes. An observed miRNP-associated RNA population consisting predominantly (>95%) of miRNAs supported the unique identity of miRNPs as biological effectors within the cell, provided clean material for analysis of changes in miRNA spectra during development, and provided strong evidence of miRNA character for a number of novel small RNAs. Likewise, the RNA spectrum derived from partial siRNP purification was useful in defining functional characteristics of this more diverse population of small RNAs.</description><subject>Animals</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Caenorhabditis elegans - metabolism</subject><subject>Caenorhabditis elegans Proteins - genetics</subject><subject>Caenorhabditis elegans Proteins - metabolism</subject><subject>MicroRNAs - analysis</subject><subject>MicroRNAs - isolation & purification</subject><subject>MicroRNAs - metabolism</subject><subject>Ribonucleoproteins - metabolism</subject><subject>RNA, Small Interfering - analysis</subject><subject>RNA, Small Interfering - isolation & purification</subject><subject>RNA, Small Interfering - metabolism</subject><issn>1355-8382</issn><issn>1469-9001</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUU1LxDAQDaK4fl38AdKTB6Fr0jRJexGW9RNEQdRrSJOpRrpJTdoF_71ZdvHjNI-ZN2-G9xA6JnhKCk7Og1NTVpEaiy20R0pe5zXGZDthylhe0aqYoP0YP1KTpvEumhDBWUFotYdeL20crNNDFmzj3ag78H3wA1iXBYgQlt6GmLU-ZAurg396mGXKmSzaFep9P3ZqsN7FLC3Mpxl08KZcPEQ7reoiHG3qAXq5vnqe3-b3jzd389l9rqngQ94wYginmraiqFSJOceGCdOokqSqWGkANDVAmaINVbg0igkBdVO2WFCj6QG6WOv2Y7MAo8ENQXWyD3ahwpf0ysr_E2ff5ZtfSlIzLHiVBE43AsF_jhAHubBRQ9cpB36MskieccbrRDxbE5MJMQZof44QLFcxyBSDXMeQyCd_3_qlbnyn39g3hYE</recordid><startdate>200709</startdate><enddate>200709</enddate><creator>Gu, Sam G</creator><creator>Pak, Julia</creator><creator>Barberan-Soler, Sergio</creator><creator>Ali, Mustapha</creator><creator>Fire, Andrew</creator><creator>Zahler, Alan M</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>200709</creationdate><title>Distinct ribonucleoprotein reservoirs for microRNA and siRNA populations in C. elegans</title><author>Gu, Sam G ; Pak, Julia ; Barberan-Soler, Sergio ; Ali, Mustapha ; Fire, Andrew ; Zahler, Alan M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-b51d163c3f728a40660d57dba41d57a54deec3de35a3b3a04da577e9b4f073dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Caenorhabditis elegans - metabolism</topic><topic>Caenorhabditis elegans Proteins - genetics</topic><topic>Caenorhabditis elegans Proteins - metabolism</topic><topic>MicroRNAs - analysis</topic><topic>MicroRNAs - isolation & purification</topic><topic>MicroRNAs - metabolism</topic><topic>Ribonucleoproteins - metabolism</topic><topic>RNA, Small Interfering - analysis</topic><topic>RNA, Small Interfering - isolation & purification</topic><topic>RNA, Small Interfering - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gu, Sam G</creatorcontrib><creatorcontrib>Pak, Julia</creatorcontrib><creatorcontrib>Barberan-Soler, Sergio</creatorcontrib><creatorcontrib>Ali, Mustapha</creatorcontrib><creatorcontrib>Fire, Andrew</creatorcontrib><creatorcontrib>Zahler, Alan M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RNA (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gu, Sam G</au><au>Pak, Julia</au><au>Barberan-Soler, Sergio</au><au>Ali, Mustapha</au><au>Fire, Andrew</au><au>Zahler, Alan M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distinct ribonucleoprotein reservoirs for microRNA and siRNA populations in C. elegans</atitle><jtitle>RNA (Cambridge)</jtitle><addtitle>RNA</addtitle><date>2007-09</date><risdate>2007</risdate><volume>13</volume><issue>9</issue><spage>1492</spage><epage>1504</epage><pages>1492-1504</pages><issn>1355-8382</issn><eissn>1469-9001</eissn><abstract>MicroRNAs (miRNAs) are regulatory molecules that share both biosynthetic derivation (cleavage from short hairpin precursor RNAs) and functional roles (downregulation of specific mRNAs through targeted degradation and/or translational inhibition). A distinct family of small RNAs, termed siRNAs, have some common characteristics but exhibit distinct modes of biosynthesis and function. In this study, we report procedures for purification of a predominant species of miRNA-containing ribonucleoprotein complexes from Caenorhabditis elegans and demonstrate that this population is distinct from the predominant pool of siRNA-containing ribonucleoprotein complexes. An observed miRNP-associated RNA population consisting predominantly (>95%) of miRNAs supported the unique identity of miRNPs as biological effectors within the cell, provided clean material for analysis of changes in miRNA spectra during development, and provided strong evidence of miRNA character for a number of novel small RNAs. Likewise, the RNA spectrum derived from partial siRNP purification was useful in defining functional characteristics of this more diverse population of small RNAs.</abstract><cop>United States</cop><pub>Cold Spring Harbor Laboratory Press</pub><pmid>17652138</pmid><doi>10.1261/rna.581907</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1355-8382 |
| ispartof | RNA (Cambridge), 2007-09, Vol.13 (9), p.1492-1504 |
| issn | 1355-8382 1469-9001 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1950768 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Caenorhabditis elegans Caenorhabditis elegans - genetics Caenorhabditis elegans - metabolism Caenorhabditis elegans Proteins - genetics Caenorhabditis elegans Proteins - metabolism MicroRNAs - analysis MicroRNAs - isolation & purification MicroRNAs - metabolism Ribonucleoproteins - metabolism RNA, Small Interfering - analysis RNA, Small Interfering - isolation & purification RNA, Small Interfering - metabolism |
| title | Distinct ribonucleoprotein reservoirs for microRNA and siRNA populations in C. elegans |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T14%3A18%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Distinct%20ribonucleoprotein%20reservoirs%20for%20microRNA%20and%20siRNA%20populations%20in%20C.%20elegans&rft.jtitle=RNA%20(Cambridge)&rft.au=Gu,%20Sam%20G&rft.date=2007-09&rft.volume=13&rft.issue=9&rft.spage=1492&rft.epage=1504&rft.pages=1492-1504&rft.issn=1355-8382&rft.eissn=1469-9001&rft_id=info:doi/10.1261/rna.581907&rft_dat=%3Cproquest_pubme%3E20136569%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20136569&rft_id=info:pmid/17652138&rfr_iscdi=true |