iGentifier: indexing and large-scale profiling of unknown transcriptomes
Development and refinement of methods to analyse differential gene expression has been essential in the progress of molecular biology. A novel approach called iGentifier is presented for profiling known and unknown transcriptomes, thus bypassing a major limitation in microarray analysis. The iGentif...
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| Veröffentlicht in: | Nucleic acids research 2007-07, Vol.35 (14), p.4640-4648 |
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| creator | Fischer, Achim Lenhard, Alistair Tronecker, Heike Lorat, Yvonne Kraenzle, Marcel Sorgenfrei, Oliver Zeppenfeld, Tim Haushalter, Michael Vogt, Gerhard Gruene, Ulrich Meyer, Annette Handlbichler, Ulrich Schweizer, Patrick Gaelweiler, Leo |
| description | Development and refinement of methods to analyse differential gene expression has been essential in the progress of molecular biology. A novel approach called iGentifier is presented for profiling known and unknown transcriptomes, thus bypassing a major limitation in microarray analysis. The iGentifier technology combines elements of fragment display (e.g. Differential Display or RMDD) and tag sequencing (e.g. SAGE, MPSS) and allows for analysis of samples in high throughput using current capillary electrophoresis equipment. Application to epidermal tissue of wild-type and mlo5 barley (Hordeum vulgare) plants, infected with powdery mildew [Blumeria graminis (DC.) E.O. Speer f.sp.hordei], led to the identification of several 100 genes induced or repressed upon infection with many well known for their response to fungal pathogens or other stressors. Ten of these genes are suggested to be classified as marker genes for durable resistance mediated by the mlo5 resistance gene. |
| doi_str_mv | 10.1093/nar/gkm331 |
| format | Article |
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A novel approach called iGentifier is presented for profiling known and unknown transcriptomes, thus bypassing a major limitation in microarray analysis. The iGentifier technology combines elements of fragment display (e.g. Differential Display or RMDD) and tag sequencing (e.g. SAGE, MPSS) and allows for analysis of samples in high throughput using current capillary electrophoresis equipment. Application to epidermal tissue of wild-type and mlo5 barley (Hordeum vulgare) plants, infected with powdery mildew [Blumeria graminis (DC.) E.O. Speer f.sp.hordei], led to the identification of several 100 genes induced or repressed upon infection with many well known for their response to fungal pathogens or other stressors. Ten of these genes are suggested to be classified as marker genes for durable resistance mediated by the mlo5 resistance gene.</description><subject>Blumeria graminis</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genomics</subject><subject>Hordeum - genetics</subject><subject>Hordeum - metabolism</subject><subject>Hordeum - microbiology</subject><subject>Hordeum vulgare</subject><subject>Reproducibility of Results</subject><subject>RNA, Plant - metabolism</subject><subject>Sequence Tagged Sites</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1v1DAQBmALgei2cOEHQIRUDkihM_5K3AMSWqCLVMGhVFRcLG_iLO4mdrATKP-erLIqHwc4-TCPXs34JeQRwgsExU68iSebbccY3iELZJLmXEl6lyyAgcgReHlADlO6BkCOgt8nB1gIhRLFgqzcmfWDa5yNp5nztb1xfpMZX2etiRubp8q0NutjaFy7m4QmG_3Wh-8-G6LxqYquH0Jn0wNyrzFtsg_37xG5fPvm43KVn384e7d8dZ5XgsohN1LQitJSsbqpbc3XWCFDpqqacrS1FFLhmlfAAYWhgq05QsOhtIUFI1jJjsjLObcf152tq2n7aFrdR9eZ-EMH4_SfE---6E34plEJEExNAc_2ATF8HW0adOdSZdvWeBvGpGWJArni_4UUKOcKdvDpX_A6jNFPvzAZkCUFvkPPZ1TFkFK0ze3KCHpXo55q1HONE378-5G_6L63CRzPIIz9v4Py2bk02JtbaeJWy4IVQq-uPmv1-urTxeq91MvJP5l9Y4I2m-iSvryggAygUAVFyX4C3-i-ZA</recordid><startdate>20070701</startdate><enddate>20070701</enddate><creator>Fischer, Achim</creator><creator>Lenhard, Alistair</creator><creator>Tronecker, Heike</creator><creator>Lorat, Yvonne</creator><creator>Kraenzle, Marcel</creator><creator>Sorgenfrei, Oliver</creator><creator>Zeppenfeld, Tim</creator><creator>Haushalter, Michael</creator><creator>Vogt, Gerhard</creator><creator>Gruene, Ulrich</creator><creator>Meyer, Annette</creator><creator>Handlbichler, Ulrich</creator><creator>Schweizer, Patrick</creator><creator>Gaelweiler, Leo</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20070701</creationdate><title>iGentifier: indexing and large-scale profiling of unknown transcriptomes</title><author>Fischer, Achim ; 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| ispartof | Nucleic acids research, 2007-07, Vol.35 (14), p.4640-4648 |
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| source | Oxford Journals Open Access Collection |
| subjects | Blumeria graminis Gene Expression Profiling - methods Gene Expression Regulation, Plant Genomics Hordeum - genetics Hordeum - metabolism Hordeum - microbiology Hordeum vulgare Reproducibility of Results RNA, Plant - metabolism Sequence Tagged Sites |
| title | iGentifier: indexing and large-scale profiling of unknown transcriptomes |
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