The IgE-facilitated allergen binding (FAB) assay: Validation of a novel flow-cytometric based method for the detection of inhibitory antibody responses

The IgE-facilitated allergen binding (IgE-FAB) assay represents an in vitro model of facilitated allergen presentation. Allergen–IgE complexes are incubated with an EBV-transformed B cell line and complexes bound to CD23 on the surface of cells are detected by flow cytometry. The addition of serum f...

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Veröffentlicht in:	Journal of immunological methods 2006-12, Vol.317 (1), p.71-79
Hauptverfasser:	Shamji, Mohamed H., Wilcock, Louisa K., Wachholz, Petra A., Dearman, Rebecca J., Kimber, Ian, Wurtzen, Peter A., Larché, Mark, Durham, Stephen R., Francis, James N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergen-specific immunotherapy Allergens - immunology Allergic rhinitis Antibody Formation - immunology Antigen Presentation - immunology Assay validation B-Lymphocytes - immunology Biological and medical sciences Desensitization, Immunologic Double-Blind Method Flow Cytometry - methods Fundamental and applied biological sciences. Psychology Fundamental immunology Humans IgE-facilitated allergen presentation IgG blocking antibodies Immunoglobulin E - immunology Molecular immunology Placebos Poaceae - immunology Pollen - immunology Protein Binding Randomized Controlled Trials as Topic Receptors, IgE - immunology Reproducibility of Results Rhinitis, Allergic, Seasonal - immunology Rhinitis, Allergic, Seasonal - prevention & control ROC Curve Sensitivity and Specificity Techniques
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