Germacrene C synthase from Lycopersicon esculentum cv. VFNT Cherry tomato: cDNA isolation, characterization, and bacterial expression of the multiple product sesquiterpene cyclase

Germacrene C was found by GC-MS and NMR analysis to be the most abundant sesquiterpene in the leaf oil of Lycopersicon esculentum cv. VFNT Cherry, with lesser amounts of germacrene A, guaia-6,9-diene, germacrene B, beta-caryophyllene, alpha-humulene, and germacrene D. Soluble enzyme preparations fro...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1998-03, Vol.95 (5), p.2216-2221
Hauptverfasser:	Colby, S.M. (University of California, Berkeley, CA.), Crock, J, Dowdle-Rizzo, B, Lemaux, P.G, Croteau, R
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ADN Alkenes Alkyl and Aryl Transferases - biosynthesis Alkyl and Aryl Transferases - chemistry Alkyl and Aryl Transferases - isolation & purification Amino Acid Sequence AMINO ACID SEQUENCES Amino acids Bacteria Biochemistry Biological Sciences BIOSINTESIS BIOSYNTHESE BIOSYNTHESIS Carbon-Carbon Lyases - biosynthesis Carbon-Carbon Lyases - chemistry Carbon-Carbon Lyases - isolation & purification CHEMICAL COMPOSITION Cloning, Molecular COMPLEMENTARY DNA COMPOSICION QUIMICA COMPOSITION CHIMIQUE Conserved Sequence Deoxyribonucleic acid Diphosphates DNA DNA Primers Enzymes ENZYMIC ACTIVITY ESCHERICHIA COLI EXPRESION GENICA EXPRESSION DES GENES FEUILLE GENBANK/AF035630 GENBANK/AF035631 GENE EXPRESSION GENE TRANSFER HOJAS LEAVES LIASAS LYASE LYASES LYCOPERSICON ESCULENTUM MOLECULAR SEQUENCE DATA MONOTERPENE MONOTERPENES MONOTERPENOS NUCLEOTIDE SEQUENCE Pentanes Plant Oils Polyisoprenyl Phosphates - metabolism Polymerase Chain Reaction Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE SESQUITERPENE SYNTHASE Sesquiterpenes Sesquiterpenes - chemistry Sesquiterpenes - metabolism SESQUITERPENOIDE SESQUITERPENOIDS SESQUITERPENOS Solanum lycopersicum - enzymology TERPENOID CYCLASE Terpenoids Tomatoes TRANSFERENCIA DE GENES TRANSFERT DE GENE
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