Lack of effect of microinjection of noradrenaline or medetomidine on stimulus‐evoked release of substance P in the spinal cord of the cat: a study with antibody microprobes
1 Experiments were performed on barbiturate anaesthetized, spinalized cats to investigate the effect of microinjected noradrenaline or medetomidine on the release of immunoreactive substance P in the dorsal spinal cord following peripheral nerve stimulation. The presence of immunoreactive substance...
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| Veröffentlicht in: | British journal of pharmacology 1994-07, Vol.112 (3), p.951-957 |
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| creator | Lang, C.W. Hope, P.J. Grubb, B.D. Duggan, A.W. |
| description | 1
Experiments were performed on barbiturate anaesthetized, spinalized cats to investigate the effect of microinjected noradrenaline or medetomidine on the release of immunoreactive substance P in the dorsal spinal cord following peripheral nerve stimulation. The presence of immunoreactive substance P was assessed with microprobes bearing C‐terminus‐directed antibodies to substance P.
2
Noradrenaline or medetomidine were microinjected into the grey matter of the spinal cord, near microprobe insertion sites, at depths of 2.5, 2.0, 1.5 and 1.0 mm below the spinal cord surface with volumes of approximately 0.125 μl and a concentration of 10−3m.
3
In the untreated spinal cord, electrical stimulation of the ipsilateral tibial nerve (suprathreshold for C‐fibres) elicited release of immunoreactive substance P which was centred in and around lamina II. Neither noradrenaline nor medetomidine administration in the manner described produced significant alterations in this pattern of nerve stimulus‐evoked release.
4
In agreement with recent ultrastructural studies these results do not support a control of substance P release by catecholamines released from sites near to the central terminals of small diameter primary afferent fibres. |
| doi_str_mv | 10.1111/j.1476-5381.1994.tb13173.x |
| format | Article |
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Experiments were performed on barbiturate anaesthetized, spinalized cats to investigate the effect of microinjected noradrenaline or medetomidine on the release of immunoreactive substance P in the dorsal spinal cord following peripheral nerve stimulation. The presence of immunoreactive substance P was assessed with microprobes bearing C‐terminus‐directed antibodies to substance P.
2
Noradrenaline or medetomidine were microinjected into the grey matter of the spinal cord, near microprobe insertion sites, at depths of 2.5, 2.0, 1.5 and 1.0 mm below the spinal cord surface with volumes of approximately 0.125 μl and a concentration of 10−3m.
3
In the untreated spinal cord, electrical stimulation of the ipsilateral tibial nerve (suprathreshold for C‐fibres) elicited release of immunoreactive substance P which was centred in and around lamina II. Neither noradrenaline nor medetomidine administration in the manner described produced significant alterations in this pattern of nerve stimulus‐evoked release.
4
In agreement with recent ultrastructural studies these results do not support a control of substance P release by catecholamines released from sites near to the central terminals of small diameter primary afferent fibres.</description><identifier>ISSN: 0007-1188</identifier><identifier>EISSN: 1476-5381</identifier><identifier>DOI: 10.1111/j.1476-5381.1994.tb13173.x</identifier><identifier>PMID: 7522862</identifier><identifier>CODEN: BJPCBM</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adrenergic alpha-Agonists - administration & dosage ; Adrenergic alpha-Agonists - pharmacology ; Animals ; Antibodies - immunology ; Antibody microprobes ; Biological and medical sciences ; Cats ; Decerebrate State - physiopathology ; Electric Stimulation ; Female ; Fundamental and applied biological sciences. Psychology ; Imidazoles - administration & dosage ; Imidazoles - pharmacology ; Iodine Radioisotopes ; Male ; Medetomidine ; microinjection ; Microinjections ; Nerve Fibers - ultrastructure ; Neurons, Afferent - physiology ; noradrenaline ; Norepinephrine - administration & dosage ; Norepinephrine - pharmacology ; peripheral nerve stimulation ; Peripheral Nerves - physiology ; Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors ; spinal cord ; Spinal Cord - drug effects ; Spinal Cord - metabolism ; Substance P - immunology ; Substance P - metabolism ; substance P release ; Substantia Gelatinosa ; Vertebrates: nervous system and sense organs</subject><ispartof>British journal of pharmacology, 1994-07, Vol.112 (3), p.951-957</ispartof><rights>1994 British Pharmacological Society</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5073-9496c68ab17f3773869ab97983332357ddb2bacded1e91078695ffdf5cf2d7e73</citedby><cites>FETCH-LOGICAL-c5073-9496c68ab17f3773869ab97983332357ddb2bacded1e91078695ffdf5cf2d7e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1910218/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1910218/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3329519$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7522862$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lang, C.W.</creatorcontrib><creatorcontrib>Hope, P.J.</creatorcontrib><creatorcontrib>Grubb, B.D.</creatorcontrib><creatorcontrib>Duggan, A.W.</creatorcontrib><title>Lack of effect of microinjection of noradrenaline or medetomidine on stimulus‐evoked release of substance P in the spinal cord of the cat: a study with antibody microprobes</title><title>British journal of pharmacology</title><addtitle>Br J Pharmacol</addtitle><description>1
Experiments were performed on barbiturate anaesthetized, spinalized cats to investigate the effect of microinjected noradrenaline or medetomidine on the release of immunoreactive substance P in the dorsal spinal cord following peripheral nerve stimulation. The presence of immunoreactive substance P was assessed with microprobes bearing C‐terminus‐directed antibodies to substance P.
2
Noradrenaline or medetomidine were microinjected into the grey matter of the spinal cord, near microprobe insertion sites, at depths of 2.5, 2.0, 1.5 and 1.0 mm below the spinal cord surface with volumes of approximately 0.125 μl and a concentration of 10−3m.
3
In the untreated spinal cord, electrical stimulation of the ipsilateral tibial nerve (suprathreshold for C‐fibres) elicited release of immunoreactive substance P which was centred in and around lamina II. Neither noradrenaline nor medetomidine administration in the manner described produced significant alterations in this pattern of nerve stimulus‐evoked release.
4
In agreement with recent ultrastructural studies these results do not support a control of substance P release by catecholamines released from sites near to the central terminals of small diameter primary afferent fibres.</description><subject>Adrenergic alpha-Agonists - administration & dosage</subject><subject>Adrenergic alpha-Agonists - pharmacology</subject><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Antibody microprobes</subject><subject>Biological and medical sciences</subject><subject>Cats</subject><subject>Decerebrate State - physiopathology</subject><subject>Electric Stimulation</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Imidazoles - administration & dosage</subject><subject>Imidazoles - pharmacology</subject><subject>Iodine Radioisotopes</subject><subject>Male</subject><subject>Medetomidine</subject><subject>microinjection</subject><subject>Microinjections</subject><subject>Nerve Fibers - ultrastructure</subject><subject>Neurons, Afferent - physiology</subject><subject>noradrenaline</subject><subject>Norepinephrine - administration & dosage</subject><subject>Norepinephrine - pharmacology</subject><subject>peripheral nerve stimulation</subject><subject>Peripheral Nerves - physiology</subject><subject>Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors</subject><subject>spinal cord</subject><subject>Spinal Cord - drug effects</subject><subject>Spinal Cord - metabolism</subject><subject>Substance P - immunology</subject><subject>Substance P - metabolism</subject><subject>substance P release</subject><subject>Substantia Gelatinosa</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0007-1188</issn><issn>1476-5381</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUUGO0zAUtRBoKIUjIFkIsUuI4yZOZoEGRsAgVWIWsLYc-5u6k9jFdmamO47ASTgUJ8FpowqWeGP__95_fvZD6AUpcpLW621OVqzOKtqQnLTtKo8doYTR_P4BWpygh2hRFAXLCGmax-hJCNuiSCCrztAZq8qyqcsF-rUW8gY7jUFrkHE6DUZ6Z-w2lcbZqWOdF8qDFb2xgJ3HAyiIbjDqUFscohnGfgy_f_yEW3cDCnvoQQSYpsPYhSisBHyNjcVxAzjsTBLD0nk1MaaWFPEci6Q0qj2-M3GDhY2mc6k6GNp510F4ih5p0Qd4Nu9L9PXD-y-XV9n688dPl2_XmawKRrN21daybkRHmKaM0aZuRdeytqGUlrRiSnVlJ6QCRaAlBUt4pbXSldSlYsDoEr056u7GLj1Wgo1e9HznzSD8njth-L-INRv-zd1ykuRK0iSBV7OAd99HCJEPJkjoe2HBjYGzmiUvyc8SnR-J6Y0heNCnS0jBp7T5lk-R8ilSPqXN57T5fRp-_rfN0-gcb8JfzrgIUvTapxhMONHSZ7QVaRPt4ki7Mz3s_8MAf3d9dTjSP28jzZw</recordid><startdate>199407</startdate><enddate>199407</enddate><creator>Lang, C.W.</creator><creator>Hope, P.J.</creator><creator>Grubb, B.D.</creator><creator>Duggan, A.W.</creator><general>Blackwell Publishing Ltd</general><general>Nature Publishing</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199407</creationdate><title>Lack of effect of microinjection of noradrenaline or medetomidine on stimulus‐evoked release of substance P in the spinal cord of the cat: a study with antibody microprobes</title><author>Lang, C.W. ; Hope, P.J. ; Grubb, B.D. ; Duggan, A.W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5073-9496c68ab17f3773869ab97983332357ddb2bacded1e91078695ffdf5cf2d7e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Adrenergic alpha-Agonists - administration & dosage</topic><topic>Adrenergic alpha-Agonists - pharmacology</topic><topic>Animals</topic><topic>Antibodies - immunology</topic><topic>Antibody microprobes</topic><topic>Biological and medical sciences</topic><topic>Cats</topic><topic>Decerebrate State - physiopathology</topic><topic>Electric Stimulation</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Imidazoles - administration & dosage</topic><topic>Imidazoles - pharmacology</topic><topic>Iodine Radioisotopes</topic><topic>Male</topic><topic>Medetomidine</topic><topic>microinjection</topic><topic>Microinjections</topic><topic>Nerve Fibers - ultrastructure</topic><topic>Neurons, Afferent - physiology</topic><topic>noradrenaline</topic><topic>Norepinephrine - administration & dosage</topic><topic>Norepinephrine - pharmacology</topic><topic>peripheral nerve stimulation</topic><topic>Peripheral Nerves - physiology</topic><topic>Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors</topic><topic>spinal cord</topic><topic>Spinal Cord - drug effects</topic><topic>Spinal Cord - metabolism</topic><topic>Substance P - immunology</topic><topic>Substance P - metabolism</topic><topic>substance P release</topic><topic>Substantia Gelatinosa</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lang, C.W.</creatorcontrib><creatorcontrib>Hope, P.J.</creatorcontrib><creatorcontrib>Grubb, B.D.</creatorcontrib><creatorcontrib>Duggan, A.W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lang, C.W.</au><au>Hope, P.J.</au><au>Grubb, B.D.</au><au>Duggan, A.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lack of effect of microinjection of noradrenaline or medetomidine on stimulus‐evoked release of substance P in the spinal cord of the cat: a study with antibody microprobes</atitle><jtitle>British journal of pharmacology</jtitle><addtitle>Br J Pharmacol</addtitle><date>1994-07</date><risdate>1994</risdate><volume>112</volume><issue>3</issue><spage>951</spage><epage>957</epage><pages>951-957</pages><issn>0007-1188</issn><eissn>1476-5381</eissn><coden>BJPCBM</coden><abstract>1
Experiments were performed on barbiturate anaesthetized, spinalized cats to investigate the effect of microinjected noradrenaline or medetomidine on the release of immunoreactive substance P in the dorsal spinal cord following peripheral nerve stimulation. The presence of immunoreactive substance P was assessed with microprobes bearing C‐terminus‐directed antibodies to substance P.
2
Noradrenaline or medetomidine were microinjected into the grey matter of the spinal cord, near microprobe insertion sites, at depths of 2.5, 2.0, 1.5 and 1.0 mm below the spinal cord surface with volumes of approximately 0.125 μl and a concentration of 10−3m.
3
In the untreated spinal cord, electrical stimulation of the ipsilateral tibial nerve (suprathreshold for C‐fibres) elicited release of immunoreactive substance P which was centred in and around lamina II. Neither noradrenaline nor medetomidine administration in the manner described produced significant alterations in this pattern of nerve stimulus‐evoked release.
4
In agreement with recent ultrastructural studies these results do not support a control of substance P release by catecholamines released from sites near to the central terminals of small diameter primary afferent fibres.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7522862</pmid><doi>10.1111/j.1476-5381.1994.tb13173.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | British journal of pharmacology, 1994-07, Vol.112 (3), p.951-957 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adrenergic alpha-Agonists - administration & dosage Adrenergic alpha-Agonists - pharmacology Animals Antibodies - immunology Antibody microprobes Biological and medical sciences Cats Decerebrate State - physiopathology Electric Stimulation Female Fundamental and applied biological sciences. Psychology Imidazoles - administration & dosage Imidazoles - pharmacology Iodine Radioisotopes Male Medetomidine microinjection Microinjections Nerve Fibers - ultrastructure Neurons, Afferent - physiology noradrenaline Norepinephrine - administration & dosage Norepinephrine - pharmacology peripheral nerve stimulation Peripheral Nerves - physiology Somesthesis and somesthetic pathways (proprioception, exteroception, nociception) interoception electrolocation. Sensory receptors spinal cord Spinal Cord - drug effects Spinal Cord - metabolism Substance P - immunology Substance P - metabolism substance P release Substantia Gelatinosa Vertebrates: nervous system and sense organs |
| title | Lack of effect of microinjection of noradrenaline or medetomidine on stimulus‐evoked release of substance P in the spinal cord of the cat: a study with antibody microprobes |
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