Improved outcome of chronic Pseudomonas aeruginosa lung infection is associated with induction of a Th1‐dominated cytokine response
SUMMARY Repeated challenge with antigen is involved in the pathogenesis of a variety of pulmonary diseases. Patients with cystic fibrosis (CF) experience recurrent pulmonary colonization with Pseudomonas aeruginosa before establishment of chronic lung infection. To mimic recurrent lung infections in...
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| Veröffentlicht in: | Clinical and experimental immunology 2002-02, Vol.127 (2), p.206-213 |
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| creator | MOSER, C. JENSEN, P. O. KOBAYASHI, O. HOUGEN, H. P. SONG, Z. RYGAARD, J. KHARAZMI, A. H?BY, N. |
| description | SUMMARY
Repeated challenge with antigen is involved in the pathogenesis of a variety of pulmonary diseases. Patients with cystic fibrosis (CF) experience recurrent pulmonary colonization with Pseudomonas aeruginosa before establishment of chronic lung
infection. To mimic recurrent lung infections in CF patients, the lungs of susceptible
BALB/c mice were re‐infected with P. aeruginosa 14 days after the initial
infection. Singly‐infected BALB/c mice, as well as non‐infected mice, were used as
controls. Decreased mortality and milder lung inflammation in re‐infected BALB/c
mice, as well as a tendency for improved clearance of bacteria, was observed when
compared with singly‐infected mice. The improved outcome in re‐infected mice correlated
with changes in CD4 cell numbers. Surface expression of LFA‐1 on pulmonary CD4 cells
was increased in re‐infected compared with singly‐infected mice. Moreover, resistance
to re‐infection was paralleled by a shift towards a Th1‐dominated response and increased
IL‐12 production. No significant increase in serum IgG was observed in the re‐infected
mice. In conclusion, these results indicate a protective role for a Th1‐dominated
response, independent of antibody production, in chronic P. aeruginosa lung infection in CF. |
| doi_str_mv | 10.1046/j.1365-2249.2002.01731.x |
| format | Article |
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Repeated challenge with antigen is involved in the pathogenesis of a variety of pulmonary diseases. Patients with cystic fibrosis (CF) experience recurrent pulmonary colonization with Pseudomonas aeruginosa before establishment of chronic lung
infection. To mimic recurrent lung infections in CF patients, the lungs of susceptible
BALB/c mice were re‐infected with P. aeruginosa 14 days after the initial
infection. Singly‐infected BALB/c mice, as well as non‐infected mice, were used as
controls. Decreased mortality and milder lung inflammation in re‐infected BALB/c
mice, as well as a tendency for improved clearance of bacteria, was observed when
compared with singly‐infected mice. The improved outcome in re‐infected mice correlated
with changes in CD4 cell numbers. Surface expression of LFA‐1 on pulmonary CD4 cells
was increased in re‐infected compared with singly‐infected mice. Moreover, resistance
to re‐infection was paralleled by a shift towards a Th1‐dominated response and increased
IL‐12 production. No significant increase in serum IgG was observed in the re‐infected
mice. In conclusion, these results indicate a protective role for a Th1‐dominated
response, independent of antibody production, in chronic P. aeruginosa lung infection in CF.</description><identifier>ISSN: 0009-9104</identifier><identifier>EISSN: 1365-2249</identifier><identifier>DOI: 10.1046/j.1365-2249.2002.01731.x</identifier><identifier>PMID: 11876741</identifier><identifier>CODEN: CEXIAL</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Agar ; Alginates ; Animal Studies ; Animals ; Antibodies, Bacterial - biosynthesis ; Antibodies, Bacterial - blood ; Bacterial diseases ; Bacterial diseases of the respiratory system ; Biological and medical sciences ; Bronchoalveolar Lavage Fluid ; CD4 Lymphocyte Count ; Chronic Disease ; cytokines ; Cytokines - biosynthesis ; Female ; Glucuronic Acid ; Hexuronic Acids ; Human bacterial diseases ; Immunoglobulin G - biosynthesis ; Immunoglobulin G - blood ; Infectious diseases ; infectious immunity‐bacteria ; Interferon-gamma - biosynthesis ; Interleukin-12 - biosynthesis ; Interleukin-4 - biosynthesis ; lung ; Lymphocyte Function-Associated Antigen-1 - biosynthesis ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Models, Animal ; Pneumonia, Bacterial - immunology ; Pseudomonas aeruginosa ; Pseudomonas Infections - immunology ; Recurrence ; rodent ; Th1 Cells - immunology ; Th1 Cells - metabolism ; Th1/Th2</subject><ispartof>Clinical and experimental immunology, 2002-02, Vol.127 (2), p.206-213</ispartof><rights>2002 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. Feb 2002</rights><rights>2002 Blackwell Science Ltd 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5081-2eda9f60e2a5ed63ad4f423ef82203f3f7523fc4dce7822da51ba54c8cb963f3</citedby><cites>FETCH-LOGICAL-c5081-2eda9f60e2a5ed63ad4f423ef82203f3f7523fc4dce7822da51ba54c8cb963f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1906339/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1906339/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,27926,27927,53793,53795</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13755314$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11876741$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MOSER, C.</creatorcontrib><creatorcontrib>JENSEN, P. O.</creatorcontrib><creatorcontrib>KOBAYASHI, O.</creatorcontrib><creatorcontrib>HOUGEN, H. P.</creatorcontrib><creatorcontrib>SONG, Z.</creatorcontrib><creatorcontrib>RYGAARD, J.</creatorcontrib><creatorcontrib>KHARAZMI, A.</creatorcontrib><creatorcontrib>H?BY, N.</creatorcontrib><title>Improved outcome of chronic Pseudomonas aeruginosa lung infection is associated with induction of a Th1‐dominated cytokine response</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>SUMMARY
Repeated challenge with antigen is involved in the pathogenesis of a variety of pulmonary diseases. Patients with cystic fibrosis (CF) experience recurrent pulmonary colonization with Pseudomonas aeruginosa before establishment of chronic lung
infection. To mimic recurrent lung infections in CF patients, the lungs of susceptible
BALB/c mice were re‐infected with P. aeruginosa 14 days after the initial
infection. Singly‐infected BALB/c mice, as well as non‐infected mice, were used as
controls. Decreased mortality and milder lung inflammation in re‐infected BALB/c
mice, as well as a tendency for improved clearance of bacteria, was observed when
compared with singly‐infected mice. The improved outcome in re‐infected mice correlated
with changes in CD4 cell numbers. Surface expression of LFA‐1 on pulmonary CD4 cells
was increased in re‐infected compared with singly‐infected mice. Moreover, resistance
to re‐infection was paralleled by a shift towards a Th1‐dominated response and increased
IL‐12 production. No significant increase in serum IgG was observed in the re‐infected
mice. In conclusion, these results indicate a protective role for a Th1‐dominated
response, independent of antibody production, in chronic P. aeruginosa lung infection in CF.</description><subject>Agar</subject><subject>Alginates</subject><subject>Animal Studies</subject><subject>Animals</subject><subject>Antibodies, Bacterial - biosynthesis</subject><subject>Antibodies, Bacterial - blood</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the respiratory system</subject><subject>Biological and medical sciences</subject><subject>Bronchoalveolar Lavage Fluid</subject><subject>CD4 Lymphocyte Count</subject><subject>Chronic Disease</subject><subject>cytokines</subject><subject>Cytokines - biosynthesis</subject><subject>Female</subject><subject>Glucuronic Acid</subject><subject>Hexuronic Acids</subject><subject>Human bacterial diseases</subject><subject>Immunoglobulin G - biosynthesis</subject><subject>Immunoglobulin G - blood</subject><subject>Infectious diseases</subject><subject>infectious immunity‐bacteria</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interleukin-12 - biosynthesis</subject><subject>Interleukin-4 - biosynthesis</subject><subject>lung</subject><subject>Lymphocyte Function-Associated Antigen-1 - biosynthesis</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Models, Animal</subject><subject>Pneumonia, Bacterial - immunology</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas Infections - immunology</subject><subject>Recurrence</subject><subject>rodent</subject><subject>Th1 Cells - immunology</subject><subject>Th1 Cells - metabolism</subject><subject>Th1/Th2</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksuO0zAUhi0EYsrAKyALCXYJvsROsgAJVQNUGgkW3Vuuc9y6JHaxk5npjg17npEnwZ1WM8AGVr783_l9OT9CmJKSkkq-3paUS1EwVrUlI4SVhNacljcP0OxOeIhmhJC2aHPFGXqS0jYvpZTsMTqjtKllXdEZ-r4YdjFcQYfDNJowAA4Wm00M3hn8OcHUhSF4nbCGOK2dD0njfvJr7LwFM7rgsctiSsE4PWabazdusthNRzG7abzc0J_ffmQn528Zsx_DF-cBR0i74BM8RY-s7hM8O43naPn-Yjn_WFx--rCYv7ssjCANLRh0urWSANMCOsl1V9mKcbANY4RbbmvBuDVVZ6DOW50WdKVFZRqzamXWz9Hbo-1uWg2QKT9G3atddIOOexW0U38q3m3UOlwp2hLJeZsNXp0MYvg6QRrV4JKBvtcewpRUTQUnlPJ_grRhNedEZPDFX-A2TNHnT8iHyjY3sKUZao6QiSGlCPbuypSoQyDUVh36rg59V4dAqNtAqJtc-vz3J98XnhKQgZcnQCejexu1Ny7dc7wWgtMqc2-O3LXrYf_fF1Dzi8Vhxn8Bgn3V8Q</recordid><startdate>200202</startdate><enddate>200202</enddate><creator>MOSER, C.</creator><creator>JENSEN, P. O.</creator><creator>KOBAYASHI, O.</creator><creator>HOUGEN, H. P.</creator><creator>SONG, Z.</creator><creator>RYGAARD, J.</creator><creator>KHARAZMI, A.</creator><creator>H?BY, N.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Oxford University Press</general><general>Blackwell Science Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200202</creationdate><title>Improved outcome of chronic Pseudomonas aeruginosa lung infection is associated with induction of a Th1‐dominated cytokine response</title><author>MOSER, C. ; JENSEN, P. O. ; KOBAYASHI, O. ; HOUGEN, H. 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O.</creatorcontrib><creatorcontrib>KOBAYASHI, O.</creatorcontrib><creatorcontrib>HOUGEN, H. P.</creatorcontrib><creatorcontrib>SONG, Z.</creatorcontrib><creatorcontrib>RYGAARD, J.</creatorcontrib><creatorcontrib>KHARAZMI, A.</creatorcontrib><creatorcontrib>H?BY, N.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical and experimental immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MOSER, C.</au><au>JENSEN, P. O.</au><au>KOBAYASHI, O.</au><au>HOUGEN, H. P.</au><au>SONG, Z.</au><au>RYGAARD, J.</au><au>KHARAZMI, A.</au><au>H?BY, N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved outcome of chronic Pseudomonas aeruginosa lung infection is associated with induction of a Th1‐dominated cytokine response</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>2002-02</date><risdate>2002</risdate><volume>127</volume><issue>2</issue><spage>206</spage><epage>213</epage><pages>206-213</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><coden>CEXIAL</coden><abstract>SUMMARY
Repeated challenge with antigen is involved in the pathogenesis of a variety of pulmonary diseases. Patients with cystic fibrosis (CF) experience recurrent pulmonary colonization with Pseudomonas aeruginosa before establishment of chronic lung
infection. To mimic recurrent lung infections in CF patients, the lungs of susceptible
BALB/c mice were re‐infected with P. aeruginosa 14 days after the initial
infection. Singly‐infected BALB/c mice, as well as non‐infected mice, were used as
controls. Decreased mortality and milder lung inflammation in re‐infected BALB/c
mice, as well as a tendency for improved clearance of bacteria, was observed when
compared with singly‐infected mice. The improved outcome in re‐infected mice correlated
with changes in CD4 cell numbers. Surface expression of LFA‐1 on pulmonary CD4 cells
was increased in re‐infected compared with singly‐infected mice. Moreover, resistance
to re‐infection was paralleled by a shift towards a Th1‐dominated response and increased
IL‐12 production. No significant increase in serum IgG was observed in the re‐infected
mice. In conclusion, these results indicate a protective role for a Th1‐dominated
response, independent of antibody production, in chronic P. aeruginosa lung infection in CF.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>11876741</pmid><doi>10.1046/j.1365-2249.2002.01731.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical and experimental immunology, 2002-02, Vol.127 (2), p.206-213 |
| issn | 0009-9104 1365-2249 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1906339 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); PubMed Central; Alma/SFX Local Collection |
| subjects | Agar Alginates Animal Studies Animals Antibodies, Bacterial - biosynthesis Antibodies, Bacterial - blood Bacterial diseases Bacterial diseases of the respiratory system Biological and medical sciences Bronchoalveolar Lavage Fluid CD4 Lymphocyte Count Chronic Disease cytokines Cytokines - biosynthesis Female Glucuronic Acid Hexuronic Acids Human bacterial diseases Immunoglobulin G - biosynthesis Immunoglobulin G - blood Infectious diseases infectious immunity‐bacteria Interferon-gamma - biosynthesis Interleukin-12 - biosynthesis Interleukin-4 - biosynthesis lung Lymphocyte Function-Associated Antigen-1 - biosynthesis Medical sciences Mice Mice, Inbred BALB C Models, Animal Pneumonia, Bacterial - immunology Pseudomonas aeruginosa Pseudomonas Infections - immunology Recurrence rodent Th1 Cells - immunology Th1 Cells - metabolism Th1/Th2 |
| title | Improved outcome of chronic Pseudomonas aeruginosa lung infection is associated with induction of a Th1‐dominated cytokine response |
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