Proliferation and telomere length in acutely mobilized blood mononuclear cells in HIV infected patients
SUMMARY The aim of the study was to investigate the mobilization of T cells in response to a stressful challenge (adrenalin stimulation), and to access T cells resided in the peripheral lymphoid organs in HIV infected patients. Seventeen patients and eight HIV seronegative controls received an adren...
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| Veröffentlicht in: | Clinical and experimental immunology 2002-03, Vol.127 (3), p.499-506 |
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| creator | SØNDERGAARD, S. R. ESSEN, M. V. SCHJERLING, P. ULLUM, H. PEDERSEN, B. K. |
| description | SUMMARY
The aim of the study was to investigate the mobilization of T cells in response to a stressful challenge (adrenalin stimulation), and to access T cells resided in the peripheral lymphoid organs in HIV infected patients. Seventeen patients and eight HIV seronegative controls received an adrenalin infusion for 1 h. Blood was sampled before, during and 1 h after adrenalin infusion. Proliferation and mean telomere restriction fragment length (telomeres) of blood mononuclear cells (BMNC) and purified CD8+ and CD4+ cells were investigated at all time points. In patients, the proliferation to pokeweed mitogens (PWM) was lower and decreased more during adrenalin infusion. After adrenalin infusion the proliferation to PWM was restored only in the controls. In all subjects telomeres in CD4+ cells declined during adrenalin infusion. Additionally, the patients had shortened telomeres in their CD8+ cells, and particularly HAART treated patients had shortened telomeres in all cell‐subtypes. The finding that patients mobilized cells with an impaired proliferation to PWM during and after adrenalin infusion has possible clinical relevance for HIV infected patients during pathological stressful conditions, such as sepsis, surgery and burns. However, this study did not find a correlation between impaired proliferation and telomeres. It is concluded that physiological stress further aggravates the HIV‐induced immune deficiency. |
| doi_str_mv | 10.1046/j.1365-2249.2002.01790.x |
| format | Article |
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The aim of the study was to investigate the mobilization of T cells in response to a stressful challenge (adrenalin stimulation), and to access T cells resided in the peripheral lymphoid organs in HIV infected patients. Seventeen patients and eight HIV seronegative controls received an adrenalin infusion for 1 h. Blood was sampled before, during and 1 h after adrenalin infusion. Proliferation and mean telomere restriction fragment length (telomeres) of blood mononuclear cells (BMNC) and purified CD8+ and CD4+ cells were investigated at all time points. In patients, the proliferation to pokeweed mitogens (PWM) was lower and decreased more during adrenalin infusion. After adrenalin infusion the proliferation to PWM was restored only in the controls. In all subjects telomeres in CD4+ cells declined during adrenalin infusion. Additionally, the patients had shortened telomeres in their CD8+ cells, and particularly HAART treated patients had shortened telomeres in all cell‐subtypes. The finding that patients mobilized cells with an impaired proliferation to PWM during and after adrenalin infusion has possible clinical relevance for HIV infected patients during pathological stressful conditions, such as sepsis, surgery and burns. However, this study did not find a correlation between impaired proliferation and telomeres. It is concluded that physiological stress further aggravates the HIV‐induced immune deficiency.</description><identifier>ISSN: 0009-9104</identifier><identifier>EISSN: 1365-2249</identifier><identifier>DOI: 10.1046/j.1365-2249.2002.01790.x</identifier><identifier>PMID: 11966767</identifier><identifier>CODEN: CEXIAL</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Adult ; Antiretroviral Therapy, Highly Active ; Biological and medical sciences ; Blood ; CD4 Lymphocyte Count ; CD4-Positive T-Lymphocytes - ultrastructure ; CD8-Positive T-Lymphocytes - ultrastructure ; Cell Movement ; Cells, Cultured ; Epinephrine - pharmacology ; Female ; HAART ; HIV ; HIV Infections - blood ; HIV Infections - diagnosis ; HIV Infections - drug therapy ; HIV Infections - immunology ; Human viral diseases ; Humans ; Infectious diseases ; Kinetics ; Lymphocyte Activation ; Male ; Medical sciences ; Middle Aged ; Original ; proliferation ; T-Lymphocytes - immunology ; T-Lymphocytes - ultrastructure ; Telomere - ultrastructure ; telomere length ; Viral diseases ; Viral diseases of the lymphoid tissue and the blood. Aids ; Viral Load</subject><ispartof>Clinical and experimental immunology, 2002-03, Vol.127 (3), p.499-506</ispartof><rights>2002 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. Mar 2002</rights><rights>2002 Blackwell Science Ltd 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5580-80d5e92b2999fc6ab03310b1bc3b89261f010592dca36a6863ed1f268cb6aafd3</citedby><cites>FETCH-LOGICAL-c5580-80d5e92b2999fc6ab03310b1bc3b89261f010592dca36a6863ed1f268cb6aafd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1906322/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1906322/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13697963$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11966767$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SØNDERGAARD, S. R.</creatorcontrib><creatorcontrib>ESSEN, M. V.</creatorcontrib><creatorcontrib>SCHJERLING, P.</creatorcontrib><creatorcontrib>ULLUM, H.</creatorcontrib><creatorcontrib>PEDERSEN, B. K.</creatorcontrib><title>Proliferation and telomere length in acutely mobilized blood mononuclear cells in HIV infected patients</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>SUMMARY
The aim of the study was to investigate the mobilization of T cells in response to a stressful challenge (adrenalin stimulation), and to access T cells resided in the peripheral lymphoid organs in HIV infected patients. Seventeen patients and eight HIV seronegative controls received an adrenalin infusion for 1 h. Blood was sampled before, during and 1 h after adrenalin infusion. Proliferation and mean telomere restriction fragment length (telomeres) of blood mononuclear cells (BMNC) and purified CD8+ and CD4+ cells were investigated at all time points. In patients, the proliferation to pokeweed mitogens (PWM) was lower and decreased more during adrenalin infusion. After adrenalin infusion the proliferation to PWM was restored only in the controls. In all subjects telomeres in CD4+ cells declined during adrenalin infusion. Additionally, the patients had shortened telomeres in their CD8+ cells, and particularly HAART treated patients had shortened telomeres in all cell‐subtypes. The finding that patients mobilized cells with an impaired proliferation to PWM during and after adrenalin infusion has possible clinical relevance for HIV infected patients during pathological stressful conditions, such as sepsis, surgery and burns. However, this study did not find a correlation between impaired proliferation and telomeres. It is concluded that physiological stress further aggravates the HIV‐induced immune deficiency.</description><subject>Adult</subject><subject>Antiretroviral Therapy, Highly Active</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>CD4 Lymphocyte Count</subject><subject>CD4-Positive T-Lymphocytes - ultrastructure</subject><subject>CD8-Positive T-Lymphocytes - ultrastructure</subject><subject>Cell Movement</subject><subject>Cells, Cultured</subject><subject>Epinephrine - pharmacology</subject><subject>Female</subject><subject>HAART</subject><subject>HIV</subject><subject>HIV Infections - blood</subject><subject>HIV Infections - diagnosis</subject><subject>HIV Infections - drug therapy</subject><subject>HIV Infections - immunology</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Kinetics</subject><subject>Lymphocyte Activation</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Original</subject><subject>proliferation</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes - ultrastructure</subject><subject>Telomere - ultrastructure</subject><subject>telomere length</subject><subject>Viral diseases</subject><subject>Viral diseases of the lymphoid tissue and the blood. Aids</subject><subject>Viral Load</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi1ERZfCX0AREtyS-mMziQ8gVatCV6pUDsDVchxn65VjL3YCXX49NrtqgQs92Z555p0ZvwgVBFcEL-F8WxEGdUnpklcUY1ph0nBc3T1Bi_vEU7TAGPOSp4pT9DzGbXoCAH2GTgnhAA00C7T5FLw1gw5yMt4V0vXFpK0fddCF1W4z3RYmhdWcovti9J2x5qfui85636e3825WVstQKG1tzPDV-ms6Bq2mxO2SrnZTfIFOBmmjfnk8z9CXD5efV1fl9c3H9eriulR13eKyxX2tOe0o53xQIDvMGMEd6RTrWk6BDJjgmtNeSQYSWmC6JwOFVnUg5dCzM_T-oLubu1H3KvUO0opdMKMMe-GlEX9nnLkVG_9dEI6BUZoE3h4Fgv826ziJ0cS8m3Taz1E0BChpluy_IGkZh6bOiq__Abd-Di79QmoK7bKhFCeoPUAq-BiDHu5HJlhkz8VWZGtFtlZkz8Vvz8VdKn3158oPhUeTE_DmCMiopB2CdMrEB44Bbzjkjd4duB_G6v2jBxCry3W-sV8xFskn</recordid><startdate>200203</startdate><enddate>200203</enddate><creator>SØNDERGAARD, S. R.</creator><creator>ESSEN, M. V.</creator><creator>SCHJERLING, P.</creator><creator>ULLUM, H.</creator><creator>PEDERSEN, B. K.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Oxford University Press</general><general>Blackwell Science Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200203</creationdate><title>Proliferation and telomere length in acutely mobilized blood mononuclear cells in HIV infected patients</title><author>SØNDERGAARD, S. R. ; ESSEN, M. V. ; SCHJERLING, P. ; ULLUM, H. ; PEDERSEN, B. K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5580-80d5e92b2999fc6ab03310b1bc3b89261f010592dca36a6863ed1f268cb6aafd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adult</topic><topic>Antiretroviral Therapy, Highly Active</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>CD4 Lymphocyte Count</topic><topic>CD4-Positive T-Lymphocytes - ultrastructure</topic><topic>CD8-Positive T-Lymphocytes - ultrastructure</topic><topic>Cell Movement</topic><topic>Cells, Cultured</topic><topic>Epinephrine - pharmacology</topic><topic>Female</topic><topic>HAART</topic><topic>HIV</topic><topic>HIV Infections - blood</topic><topic>HIV Infections - diagnosis</topic><topic>HIV Infections - drug therapy</topic><topic>HIV Infections - immunology</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Kinetics</topic><topic>Lymphocyte Activation</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Original</topic><topic>proliferation</topic><topic>T-Lymphocytes - immunology</topic><topic>T-Lymphocytes - ultrastructure</topic><topic>Telomere - ultrastructure</topic><topic>telomere length</topic><topic>Viral diseases</topic><topic>Viral diseases of the lymphoid tissue and the blood. Aids</topic><topic>Viral Load</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SØNDERGAARD, S. R.</creatorcontrib><creatorcontrib>ESSEN, M. V.</creatorcontrib><creatorcontrib>SCHJERLING, P.</creatorcontrib><creatorcontrib>ULLUM, H.</creatorcontrib><creatorcontrib>PEDERSEN, B. K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical and experimental immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SØNDERGAARD, S. R.</au><au>ESSEN, M. V.</au><au>SCHJERLING, P.</au><au>ULLUM, H.</au><au>PEDERSEN, B. K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proliferation and telomere length in acutely mobilized blood mononuclear cells in HIV infected patients</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>2002-03</date><risdate>2002</risdate><volume>127</volume><issue>3</issue><spage>499</spage><epage>506</epage><pages>499-506</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><coden>CEXIAL</coden><abstract>SUMMARY
The aim of the study was to investigate the mobilization of T cells in response to a stressful challenge (adrenalin stimulation), and to access T cells resided in the peripheral lymphoid organs in HIV infected patients. Seventeen patients and eight HIV seronegative controls received an adrenalin infusion for 1 h. Blood was sampled before, during and 1 h after adrenalin infusion. Proliferation and mean telomere restriction fragment length (telomeres) of blood mononuclear cells (BMNC) and purified CD8+ and CD4+ cells were investigated at all time points. In patients, the proliferation to pokeweed mitogens (PWM) was lower and decreased more during adrenalin infusion. After adrenalin infusion the proliferation to PWM was restored only in the controls. In all subjects telomeres in CD4+ cells declined during adrenalin infusion. Additionally, the patients had shortened telomeres in their CD8+ cells, and particularly HAART treated patients had shortened telomeres in all cell‐subtypes. The finding that patients mobilized cells with an impaired proliferation to PWM during and after adrenalin infusion has possible clinical relevance for HIV infected patients during pathological stressful conditions, such as sepsis, surgery and burns. However, this study did not find a correlation between impaired proliferation and telomeres. It is concluded that physiological stress further aggravates the HIV‐induced immune deficiency.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>11966767</pmid><doi>10.1046/j.1365-2249.2002.01790.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adult Antiretroviral Therapy, Highly Active Biological and medical sciences Blood CD4 Lymphocyte Count CD4-Positive T-Lymphocytes - ultrastructure CD8-Positive T-Lymphocytes - ultrastructure Cell Movement Cells, Cultured Epinephrine - pharmacology Female HAART HIV HIV Infections - blood HIV Infections - diagnosis HIV Infections - drug therapy HIV Infections - immunology Human viral diseases Humans Infectious diseases Kinetics Lymphocyte Activation Male Medical sciences Middle Aged Original proliferation T-Lymphocytes - immunology T-Lymphocytes - ultrastructure Telomere - ultrastructure telomere length Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids Viral Load |
| title | Proliferation and telomere length in acutely mobilized blood mononuclear cells in HIV infected patients |
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