Impaired production of proinflammatory cytokines in response to lipopolysaccharide (LPS) stimulation in elderly humans
Ageing is associated with decreased resistance to bacterial infections and concomitant increased circulating levels of inflammatory cytokines. The purpose of the present study was to research age‐related changes in levels of early mediators of the acute‐phase response in whole blood supernatants fol...
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| Veröffentlicht in: | Clinical and experimental immunology 1999-11, Vol.118 (2), p.235-241 |
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| creator | BRUUNSGAARD, H PEDERSEN, A. N SCHROLL, M SKINHØJ, P PEDERSEN, B. K |
| description | Ageing is associated with decreased resistance to bacterial infections and concomitant increased circulating levels of inflammatory cytokines. The purpose of the present study was to research age‐related changes in levels of early mediators of the acute‐phase response in whole blood supernatants following LPS stimulation, representing an ex vivo model of sepsis. Levels of tumour necrosis factor‐alpha (TNF‐α), IL‐1β and IL‐6 in whole blood supernatants were measured after in vitro LPS stimulation for 24 h in 168 elderly humans aged 81 years from the 1914 cohort in Glostrup, Denmark and in 91 young controls aged 19–31 years. Levels of TNF‐α and IL‐1β were significantly lower in elderly humans compared with young controls, whereas no difference was detected with regard to IL‐6. Elderly humans with low body mass index had the lowest levels of IL‐1β. Young women had lower levels of proinflammatory cytokines compared with young men, but this difference was blurred by ageing. No relation was found between circulating plasma levels of TNF‐α and levels after in vitro LPS stimulation. In conclusion, decreased production of TNF‐α and IL‐1β after exposure to LPS may reflect impaired host defence against infections in the elderly and be of importance in elderly humans with underlying health disorders. However, the clinical relevance is questionable in healthy elderly people because decreased levels were found compared with young men but not compared with young women. |
| doi_str_mv | 10.1046/j.1365-2249.1999.01045.x |
| format | Article |
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N ; SCHROLL, M ; SKINHØJ, P ; PEDERSEN, B. K</creator><creatorcontrib>BRUUNSGAARD, H ; PEDERSEN, A. N ; SCHROLL, M ; SKINHØJ, P ; PEDERSEN, B. K</creatorcontrib><description>Ageing is associated with decreased resistance to bacterial infections and concomitant increased circulating levels of inflammatory cytokines. The purpose of the present study was to research age‐related changes in levels of early mediators of the acute‐phase response in whole blood supernatants following LPS stimulation, representing an ex vivo model of sepsis. Levels of tumour necrosis factor‐alpha (TNF‐α), IL‐1β and IL‐6 in whole blood supernatants were measured after in vitro LPS stimulation for 24 h in 168 elderly humans aged 81 years from the 1914 cohort in Glostrup, Denmark and in 91 young controls aged 19–31 years. Levels of TNF‐α and IL‐1β were significantly lower in elderly humans compared with young controls, whereas no difference was detected with regard to IL‐6. Elderly humans with low body mass index had the lowest levels of IL‐1β. Young women had lower levels of proinflammatory cytokines compared with young men, but this difference was blurred by ageing. No relation was found between circulating plasma levels of TNF‐α and levels after in vitro LPS stimulation. In conclusion, decreased production of TNF‐α and IL‐1β after exposure to LPS may reflect impaired host defence against infections in the elderly and be of importance in elderly humans with underlying health disorders. However, the clinical relevance is questionable in healthy elderly people because decreased levels were found compared with young men but not compared with young women.</description><identifier>ISSN: 0009-9104</identifier><identifier>EISSN: 1365-2249</identifier><identifier>DOI: 10.1046/j.1365-2249.1999.01045.x</identifier><identifier>PMID: 10540184</identifier><identifier>CODEN: CEXIAL</identifier><language>eng</language><publisher>Oxford BSL: Blackwell Science Ltd</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; ageing ; Arteriosclerosis - immunology ; Arteriosclerosis - pathology ; Biological and medical sciences ; Body Mass Index ; Cytokines - biosynthesis ; Denmark ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; General aspects. Ontogeny. Phylogeny ; Humans ; IL‐1β ; IL‐6 ; Immunobiology ; Inflammation - immunology ; interleukin 1^b ; Interleukin-1 - biosynthesis ; Interleukin-1 - blood ; Interleukin-6 - biosynthesis ; Interleukin-6 - blood ; Interleukin-6 - immunology ; lipopolysaccharide ; lipopolysaccharides ; Lipopolysaccharides - immunology ; Lymphocyte Activation - immunology ; Lymphocyte Subsets - immunology ; Male ; Original ; tumor necrosis factor-^a ; Tumor Necrosis Factor-alpha - biosynthesis ; Tumor Necrosis Factor-alpha - metabolism ; tumour necrosis factor‐alpha</subject><ispartof>Clinical and experimental immunology, 1999-11, Vol.118 (2), p.235-241</ispartof><rights>Blackwell Science Ltd, Oxford</rights><rights>1999 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. 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N</creatorcontrib><creatorcontrib>SCHROLL, M</creatorcontrib><creatorcontrib>SKINHØJ, P</creatorcontrib><creatorcontrib>PEDERSEN, B. K</creatorcontrib><title>Impaired production of proinflammatory cytokines in response to lipopolysaccharide (LPS) stimulation in elderly humans</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>Ageing is associated with decreased resistance to bacterial infections and concomitant increased circulating levels of inflammatory cytokines. The purpose of the present study was to research age‐related changes in levels of early mediators of the acute‐phase response in whole blood supernatants following LPS stimulation, representing an ex vivo model of sepsis. Levels of tumour necrosis factor‐alpha (TNF‐α), IL‐1β and IL‐6 in whole blood supernatants were measured after in vitro LPS stimulation for 24 h in 168 elderly humans aged 81 years from the 1914 cohort in Glostrup, Denmark and in 91 young controls aged 19–31 years. Levels of TNF‐α and IL‐1β were significantly lower in elderly humans compared with young controls, whereas no difference was detected with regard to IL‐6. Elderly humans with low body mass index had the lowest levels of IL‐1β. Young women had lower levels of proinflammatory cytokines compared with young men, but this difference was blurred by ageing. No relation was found between circulating plasma levels of TNF‐α and levels after in vitro LPS stimulation. In conclusion, decreased production of TNF‐α and IL‐1β after exposure to LPS may reflect impaired host defence against infections in the elderly and be of importance in elderly humans with underlying health disorders. However, the clinical relevance is questionable in healthy elderly people because decreased levels were found compared with young men but not compared with young women.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>ageing</subject><subject>Arteriosclerosis - immunology</subject><subject>Arteriosclerosis - pathology</subject><subject>Biological and medical sciences</subject><subject>Body Mass Index</subject><subject>Cytokines - biosynthesis</subject><subject>Denmark</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>General aspects. Ontogeny. Phylogeny</subject><subject>Humans</subject><subject>IL‐1β</subject><subject>IL‐6</subject><subject>Immunobiology</subject><subject>Inflammation - immunology</subject><subject>interleukin 1^b</subject><subject>Interleukin-1 - biosynthesis</subject><subject>Interleukin-1 - blood</subject><subject>Interleukin-6 - biosynthesis</subject><subject>Interleukin-6 - blood</subject><subject>Interleukin-6 - immunology</subject><subject>lipopolysaccharide</subject><subject>lipopolysaccharides</subject><subject>Lipopolysaccharides - immunology</subject><subject>Lymphocyte Activation - immunology</subject><subject>Lymphocyte Subsets - immunology</subject><subject>Male</subject><subject>Original</subject><subject>tumor necrosis factor-^a</subject><subject>Tumor Necrosis Factor-alpha - biosynthesis</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>tumour necrosis factor‐alpha</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkl2P1CAUhonRuOPqXzDEGKMXrUApLReamMmqk0yiiXpNGKAOI4UutOv230t3JuvqjV7xcZ7znk8AIEYlRpS9PpS4YnVBCOUl5pyXKH_X5fU9sLo13AcrhBAveDadgUcpHfKTMUYegjOMaopwS1fgatMP0kaj4RCDntRog4ehW17Wd072vRxDnKGax_DDepOg9TCaNASfDBwDdHYIQ3BzkkrtZbTawJfbz19ewTTafnLyRjD7GKdNdDPcT7306TF40EmXzJPTeQ6-vb_4uv5YbD992KzfbQvFSFUXVUN3tWaMK6wR6WqtKsY7zVCzY6xqOVOyJTuFOcM4Yw0istYdyWXLmhtNqnPw9qg7TLveaGX8GKUTQ7S9jLMI0oo_Ld7uxfdwJTDPLSIsC7w4CcRwOZk0it4mZZyT3oQpCcYJwRWt_gnihlJSNzyDz_4CD2GKPnchB2VtroQsebdHSMWQUjTdbcoYiWUFxEEskxbLpMWyAuJmBcR1dn16t-Q7jseZZ-D5CZBJSddF6ZVNvznOCW2Xyt8csZ_Wmfm_44v1xWa5Vb8A_PPOpQ</recordid><startdate>199911</startdate><enddate>199911</enddate><creator>BRUUNSGAARD, H</creator><creator>PEDERSEN, A. N</creator><creator>SCHROLL, M</creator><creator>SKINHØJ, P</creator><creator>PEDERSEN, B. K</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Oxford University Press</general><general>Blackwell Science Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199911</creationdate><title>Impaired production of proinflammatory cytokines in response to lipopolysaccharide (LPS) stimulation in elderly humans</title><author>BRUUNSGAARD, H ; PEDERSEN, A. N ; SCHROLL, M ; SKINHØJ, P ; PEDERSEN, B. K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6235-374b5d669c1d02f5dc369fd607b663896ca82bc19611d66702a5df2009a59ed23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>ageing</topic><topic>Arteriosclerosis - immunology</topic><topic>Arteriosclerosis - pathology</topic><topic>Biological and medical sciences</topic><topic>Body Mass Index</topic><topic>Cytokines - biosynthesis</topic><topic>Denmark</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>General aspects. Ontogeny. Phylogeny</topic><topic>Humans</topic><topic>IL‐1β</topic><topic>IL‐6</topic><topic>Immunobiology</topic><topic>Inflammation - immunology</topic><topic>interleukin 1^b</topic><topic>Interleukin-1 - biosynthesis</topic><topic>Interleukin-1 - blood</topic><topic>Interleukin-6 - biosynthesis</topic><topic>Interleukin-6 - blood</topic><topic>Interleukin-6 - immunology</topic><topic>lipopolysaccharide</topic><topic>lipopolysaccharides</topic><topic>Lipopolysaccharides - immunology</topic><topic>Lymphocyte Activation - immunology</topic><topic>Lymphocyte Subsets - immunology</topic><topic>Male</topic><topic>Original</topic><topic>tumor necrosis factor-^a</topic><topic>Tumor Necrosis Factor-alpha - biosynthesis</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>tumour necrosis factor‐alpha</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BRUUNSGAARD, H</creatorcontrib><creatorcontrib>PEDERSEN, A. N</creatorcontrib><creatorcontrib>SCHROLL, M</creatorcontrib><creatorcontrib>SKINHØJ, P</creatorcontrib><creatorcontrib>PEDERSEN, B. K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical and experimental immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BRUUNSGAARD, H</au><au>PEDERSEN, A. N</au><au>SCHROLL, M</au><au>SKINHØJ, P</au><au>PEDERSEN, B. K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impaired production of proinflammatory cytokines in response to lipopolysaccharide (LPS) stimulation in elderly humans</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>1999-11</date><risdate>1999</risdate><volume>118</volume><issue>2</issue><spage>235</spage><epage>241</epage><pages>235-241</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><coden>CEXIAL</coden><abstract>Ageing is associated with decreased resistance to bacterial infections and concomitant increased circulating levels of inflammatory cytokines. The purpose of the present study was to research age‐related changes in levels of early mediators of the acute‐phase response in whole blood supernatants following LPS stimulation, representing an ex vivo model of sepsis. Levels of tumour necrosis factor‐alpha (TNF‐α), IL‐1β and IL‐6 in whole blood supernatants were measured after in vitro LPS stimulation for 24 h in 168 elderly humans aged 81 years from the 1914 cohort in Glostrup, Denmark and in 91 young controls aged 19–31 years. Levels of TNF‐α and IL‐1β were significantly lower in elderly humans compared with young controls, whereas no difference was detected with regard to IL‐6. Elderly humans with low body mass index had the lowest levels of IL‐1β. Young women had lower levels of proinflammatory cytokines compared with young men, but this difference was blurred by ageing. No relation was found between circulating plasma levels of TNF‐α and levels after in vitro LPS stimulation. In conclusion, decreased production of TNF‐α and IL‐1β after exposure to LPS may reflect impaired host defence against infections in the elderly and be of importance in elderly humans with underlying health disorders. However, the clinical relevance is questionable in healthy elderly people because decreased levels were found compared with young men but not compared with young women.</abstract><cop>Oxford BSL</cop><pub>Blackwell Science Ltd</pub><pmid>10540184</pmid><doi>10.1046/j.1365-2249.1999.01045.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adolescent Adult Aged Aged, 80 and over ageing Arteriosclerosis - immunology Arteriosclerosis - pathology Biological and medical sciences Body Mass Index Cytokines - biosynthesis Denmark Female Fundamental and applied biological sciences. Psychology Fundamental immunology General aspects. Ontogeny. Phylogeny Humans IL‐1β IL‐6 Immunobiology Inflammation - immunology interleukin 1^b Interleukin-1 - biosynthesis Interleukin-1 - blood Interleukin-6 - biosynthesis Interleukin-6 - blood Interleukin-6 - immunology lipopolysaccharide lipopolysaccharides Lipopolysaccharides - immunology Lymphocyte Activation - immunology Lymphocyte Subsets - immunology Male Original tumor necrosis factor-^a Tumor Necrosis Factor-alpha - biosynthesis Tumor Necrosis Factor-alpha - metabolism tumour necrosis factor‐alpha |
| title | Impaired production of proinflammatory cytokines in response to lipopolysaccharide (LPS) stimulation in elderly humans |
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