Evidence of idiotypic modulation in the immune response to gp43, the major antigenic component of Paracoccidioides brasiliensis in both mice and humans

Paracoccidioidomycosis (PCM) is a systemic mycosis endemic in Latin America, with a high prevalence in Brazil, Argentina, Colombia and Venezuela. The aetiologic agent of disease is a thermal dimorphic fungus, Paracoccidioides brasiliensis. A glycoprotein of 43 000 D (gp43) is the major antigen of P....

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Veröffentlicht in:Clinical and experimental immunology 1998-10, Vol.114 (1), p.40-48
Hauptverfasser: SOUZA, A. R, GESZTESI, J.-L, MORAES, J. Z, CRUZ, C. R. B, SATO, J, MARIANO, M, LOPES, J. D
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container_issue 1
container_start_page 40
container_title Clinical and experimental immunology
container_volume 114
creator SOUZA, A. R
GESZTESI, J.-L
MORAES, J. Z
CRUZ, C. R. B
SATO, J
MARIANO, M
LOPES, J. D
description Paracoccidioidomycosis (PCM) is a systemic mycosis endemic in Latin America, with a high prevalence in Brazil, Argentina, Colombia and Venezuela. The aetiologic agent of disease is a thermal dimorphic fungus, Paracoccidioides brasiliensis. A glycoprotein of 43 000 D (gp43) is the major antigen of P. brasiliensis. Antibodies directed to this antigen are detected in the sera of all patients with PCM. Gp43 binds to laminin, thus participating in adhesion, invasion and pathogenesis of the fungus. As the role of antibodies in PCM is not fully understood, we decided to investigate the outcome of mice immunization with three distinct anti‐gp43 MoAbs (17c, 8a and 24a) coupled with keyhole limpet haemocyanin (KLH). Results show not only the expected presence of anti‐Id (AB2) antibodies in the sera of these animals but also a spontaneous and increasing amount of anti‐anti‐Id (AB3) antibodies after the third course of immunization. Hybridomas producing both AB2 and AB3 MoAbs were obtained using spleen cells from mice immunized with MoAb 17c. AB3 MoAbs were also obtained with spleen cells of mice immunized with MoAbs 8a and 24a. It was also shown that human PCM patients' sera with high titres of anti‐gp43 antibodies generate anti‐Id antibodies. These data suggest that the immune response to P. brasiliensis can be spontaneously modulated by the idiotypic network.
doi_str_mv 10.1046/j.1365-2249.1998.00679.x
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R ; GESZTESI, J.-L ; MORAES, J. Z ; CRUZ, C. R. B ; SATO, J ; MARIANO, M ; LOPES, J. D</creator><creatorcontrib>SOUZA, A. R ; GESZTESI, J.-L ; MORAES, J. Z ; CRUZ, C. R. B ; SATO, J ; MARIANO, M ; LOPES, J. D</creatorcontrib><description>Paracoccidioidomycosis (PCM) is a systemic mycosis endemic in Latin America, with a high prevalence in Brazil, Argentina, Colombia and Venezuela. The aetiologic agent of disease is a thermal dimorphic fungus, Paracoccidioides brasiliensis. A glycoprotein of 43 000 D (gp43) is the major antigen of P. brasiliensis. Antibodies directed to this antigen are detected in the sera of all patients with PCM. Gp43 binds to laminin, thus participating in adhesion, invasion and pathogenesis of the fungus. As the role of antibodies in PCM is not fully understood, we decided to investigate the outcome of mice immunization with three distinct anti‐gp43 MoAbs (17c, 8a and 24a) coupled with keyhole limpet haemocyanin (KLH). Results show not only the expected presence of anti‐Id (AB2) antibodies in the sera of these animals but also a spontaneous and increasing amount of anti‐anti‐Id (AB3) antibodies after the third course of immunization. Hybridomas producing both AB2 and AB3 MoAbs were obtained using spleen cells from mice immunized with MoAb 17c. AB3 MoAbs were also obtained with spleen cells of mice immunized with MoAbs 8a and 24a. It was also shown that human PCM patients' sera with high titres of anti‐gp43 antibodies generate anti‐Id antibodies. 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D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence of idiotypic modulation in the immune response to gp43, the major antigenic component of Paracoccidioides brasiliensis in both mice and humans</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>1998-10</date><risdate>1998</risdate><volume>114</volume><issue>1</issue><spage>40</spage><epage>48</epage><pages>40-48</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><coden>CEXIAL</coden><abstract>Paracoccidioidomycosis (PCM) is a systemic mycosis endemic in Latin America, with a high prevalence in Brazil, Argentina, Colombia and Venezuela. The aetiologic agent of disease is a thermal dimorphic fungus, Paracoccidioides brasiliensis. A glycoprotein of 43 000 D (gp43) is the major antigen of P. brasiliensis. Antibodies directed to this antigen are detected in the sera of all patients with PCM. Gp43 binds to laminin, thus participating in adhesion, invasion and pathogenesis of the fungus. As the role of antibodies in PCM is not fully understood, we decided to investigate the outcome of mice immunization with three distinct anti‐gp43 MoAbs (17c, 8a and 24a) coupled with keyhole limpet haemocyanin (KLH). Results show not only the expected presence of anti‐Id (AB2) antibodies in the sera of these animals but also a spontaneous and increasing amount of anti‐anti‐Id (AB3) antibodies after the third course of immunization. Hybridomas producing both AB2 and AB3 MoAbs were obtained using spleen cells from mice immunized with MoAb 17c. AB3 MoAbs were also obtained with spleen cells of mice immunized with MoAbs 8a and 24a. It was also shown that human PCM patients' sera with high titres of anti‐gp43 antibodies generate anti‐Id antibodies. These data suggest that the immune response to P. brasiliensis can be spontaneously modulated by the idiotypic network.</abstract><cop>Oxford BSL</cop><pub>Blackwell Science Ltd</pub><pmid>9764601</pmid><doi>10.1046/j.1365-2249.1998.00679.x</doi><tpages>9</tpages></addata></record>
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subjects Animals
Antibodies, Anti-Idiotypic - immunology
Antibodies, Fungal - immunology
Antibodies, Monoclonal - immunology
Antigens, Fungal - immunology
Biological and medical sciences
Carcinoembryonic Antigen - immunology
Female
Fungal Proteins
General aspects
Glycoproteins - immunology
gp43
human infection
Humans
Hybridomas
idiotypic network
Immunization, Passive
Immunoglobulin Idiotypes - blood
Immunoglobulin Idiotypes - immunology
Medical sciences
Mice
Mice, Inbred BALB C
monoclonal antibodies
Oligosaccharides - immunology
Original
Paracoccidioides - immunology
Paracoccidioides brasiliensis
Paracoccidioidomycosis - blood
Paracoccidioidomycosis - immunology
title Evidence of idiotypic modulation in the immune response to gp43, the major antigenic component of Paracoccidioides brasiliensis in both mice and humans
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