Evidence of idiotypic modulation in the immune response to gp43, the major antigenic component of Paracoccidioides brasiliensis in both mice and humans
Paracoccidioidomycosis (PCM) is a systemic mycosis endemic in Latin America, with a high prevalence in Brazil, Argentina, Colombia and Venezuela. The aetiologic agent of disease is a thermal dimorphic fungus, Paracoccidioides brasiliensis. A glycoprotein of 43 000 D (gp43) is the major antigen of P....
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description | Paracoccidioidomycosis (PCM) is a systemic mycosis endemic in Latin America, with a high prevalence in Brazil, Argentina, Colombia and Venezuela. The aetiologic agent of disease is a thermal dimorphic fungus, Paracoccidioides brasiliensis. A glycoprotein of 43 000 D (gp43) is the major antigen of P. brasiliensis. Antibodies directed to this antigen are detected in the sera of all patients with PCM. Gp43 binds to laminin, thus participating in adhesion, invasion and pathogenesis of the fungus. As the role of antibodies in PCM is not fully understood, we decided to investigate the outcome of mice immunization with three distinct anti‐gp43 MoAbs (17c, 8a and 24a) coupled with keyhole limpet haemocyanin (KLH). Results show not only the expected presence of anti‐Id (AB2) antibodies in the sera of these animals but also a spontaneous and increasing amount of anti‐anti‐Id (AB3) antibodies after the third course of immunization. Hybridomas producing both AB2 and AB3 MoAbs were obtained using spleen cells from mice immunized with MoAb 17c. AB3 MoAbs were also obtained with spleen cells of mice immunized with MoAbs 8a and 24a. It was also shown that human PCM patients' sera with high titres of anti‐gp43 antibodies generate anti‐Id antibodies. These data suggest that the immune response to P. brasiliensis can be spontaneously modulated by the idiotypic network. |
doi_str_mv | 10.1046/j.1365-2249.1998.00679.x |
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R ; GESZTESI, J.-L ; MORAES, J. Z ; CRUZ, C. R. B ; SATO, J ; MARIANO, M ; LOPES, J. D</creator><creatorcontrib>SOUZA, A. R ; GESZTESI, J.-L ; MORAES, J. Z ; CRUZ, C. R. B ; SATO, J ; MARIANO, M ; LOPES, J. D</creatorcontrib><description>Paracoccidioidomycosis (PCM) is a systemic mycosis endemic in Latin America, with a high prevalence in Brazil, Argentina, Colombia and Venezuela. The aetiologic agent of disease is a thermal dimorphic fungus, Paracoccidioides brasiliensis. A glycoprotein of 43 000 D (gp43) is the major antigen of P. brasiliensis. Antibodies directed to this antigen are detected in the sera of all patients with PCM. Gp43 binds to laminin, thus participating in adhesion, invasion and pathogenesis of the fungus. As the role of antibodies in PCM is not fully understood, we decided to investigate the outcome of mice immunization with three distinct anti‐gp43 MoAbs (17c, 8a and 24a) coupled with keyhole limpet haemocyanin (KLH). Results show not only the expected presence of anti‐Id (AB2) antibodies in the sera of these animals but also a spontaneous and increasing amount of anti‐anti‐Id (AB3) antibodies after the third course of immunization. Hybridomas producing both AB2 and AB3 MoAbs were obtained using spleen cells from mice immunized with MoAb 17c. AB3 MoAbs were also obtained with spleen cells of mice immunized with MoAbs 8a and 24a. It was also shown that human PCM patients' sera with high titres of anti‐gp43 antibodies generate anti‐Id antibodies. These data suggest that the immune response to P. brasiliensis can be spontaneously modulated by the idiotypic network.</description><identifier>ISSN: 0009-9104</identifier><identifier>EISSN: 1365-2249</identifier><identifier>DOI: 10.1046/j.1365-2249.1998.00679.x</identifier><identifier>PMID: 9764601</identifier><identifier>CODEN: CEXIAL</identifier><language>eng</language><publisher>Oxford BSL: Blackwell Science Ltd</publisher><subject>Animals ; Antibodies, Anti-Idiotypic - immunology ; Antibodies, Fungal - immunology ; Antibodies, Monoclonal - immunology ; Antigens, Fungal - immunology ; Biological and medical sciences ; Carcinoembryonic Antigen - immunology ; Female ; Fungal Proteins ; General aspects ; Glycoproteins - immunology ; gp43 ; human infection ; Humans ; Hybridomas ; idiotypic network ; Immunization, Passive ; Immunoglobulin Idiotypes - blood ; Immunoglobulin Idiotypes - immunology ; Medical sciences ; Mice ; Mice, Inbred BALB C ; monoclonal antibodies ; Oligosaccharides - immunology ; Original ; Paracoccidioides - immunology ; Paracoccidioides brasiliensis ; Paracoccidioidomycosis - blood ; Paracoccidioidomycosis - immunology</subject><ispartof>Clinical and experimental immunology, 1998-10, Vol.114 (1), p.40-48</ispartof><rights>Blackwell Science Ltd, Oxford</rights><rights>1998 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. Oct 1998</rights><rights>1998 Blackwell Science Ltd 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5059-b14d3e91a92d329f7232ec5b028fb7609008461ed1bf2a83d2222208167322213</citedby><cites>FETCH-LOGICAL-c5059-b14d3e91a92d329f7232ec5b028fb7609008461ed1bf2a83d2222208167322213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1905082/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1905082/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2396033$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9764601$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SOUZA, A. R</creatorcontrib><creatorcontrib>GESZTESI, J.-L</creatorcontrib><creatorcontrib>MORAES, J. Z</creatorcontrib><creatorcontrib>CRUZ, C. R. B</creatorcontrib><creatorcontrib>SATO, J</creatorcontrib><creatorcontrib>MARIANO, M</creatorcontrib><creatorcontrib>LOPES, J. D</creatorcontrib><title>Evidence of idiotypic modulation in the immune response to gp43, the major antigenic component of Paracoccidioides brasiliensis in both mice and humans</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>Paracoccidioidomycosis (PCM) is a systemic mycosis endemic in Latin America, with a high prevalence in Brazil, Argentina, Colombia and Venezuela. The aetiologic agent of disease is a thermal dimorphic fungus, Paracoccidioides brasiliensis. A glycoprotein of 43 000 D (gp43) is the major antigen of P. brasiliensis. Antibodies directed to this antigen are detected in the sera of all patients with PCM. Gp43 binds to laminin, thus participating in adhesion, invasion and pathogenesis of the fungus. As the role of antibodies in PCM is not fully understood, we decided to investigate the outcome of mice immunization with three distinct anti‐gp43 MoAbs (17c, 8a and 24a) coupled with keyhole limpet haemocyanin (KLH). Results show not only the expected presence of anti‐Id (AB2) antibodies in the sera of these animals but also a spontaneous and increasing amount of anti‐anti‐Id (AB3) antibodies after the third course of immunization. Hybridomas producing both AB2 and AB3 MoAbs were obtained using spleen cells from mice immunized with MoAb 17c. AB3 MoAbs were also obtained with spleen cells of mice immunized with MoAbs 8a and 24a. It was also shown that human PCM patients' sera with high titres of anti‐gp43 antibodies generate anti‐Id antibodies. These data suggest that the immune response to P. brasiliensis can be spontaneously modulated by the idiotypic network.</description><subject>Animals</subject><subject>Antibodies, Anti-Idiotypic - immunology</subject><subject>Antibodies, Fungal - immunology</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, Fungal - immunology</subject><subject>Biological and medical sciences</subject><subject>Carcinoembryonic Antigen - immunology</subject><subject>Female</subject><subject>Fungal Proteins</subject><subject>General aspects</subject><subject>Glycoproteins - immunology</subject><subject>gp43</subject><subject>human infection</subject><subject>Humans</subject><subject>Hybridomas</subject><subject>idiotypic network</subject><subject>Immunization, Passive</subject><subject>Immunoglobulin Idiotypes - blood</subject><subject>Immunoglobulin Idiotypes - immunology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>monoclonal antibodies</subject><subject>Oligosaccharides - immunology</subject><subject>Original</subject><subject>Paracoccidioides - immunology</subject><subject>Paracoccidioides brasiliensis</subject><subject>Paracoccidioidomycosis - blood</subject><subject>Paracoccidioidomycosis - immunology</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkt-K1DAUxoso67j6CEIQ8crW_GuagAgyjLqwoBd6HdI0nUlpkjFp150n2dfddGcY1BvNTRK-3_nOSfiKAiBYIUjZu6FChNUlxlRUSAheQcgaUd0-KlZn4XGxghCKUuSKp8WzlIZ8ZYzhi-JCNIwyiFbF3ebGdsZrA0IPbGfDdNhbDVzo5lFNNnhgPZh2BljnZm9ANGkffDJgCmC7p-Ttg-jUECJQfrJb43O5Di5Txk-L6zcVlQ5aL-65VwJtVMmO1vhk02LfhmkHnM0zKN-B3eyUT8-LJ70ak3lx2i-LH58239dfyuuvn6_WH69LXcNalC2iHTECKYE7gkXfYIKNrluIed82DAoIOWXIdKjtseKkw8uCHLGG5AMil8WHo-9-bp3pdJ45qlHuo3UqHmRQVv6peLuT23AjkYA15DgbvDkZxPBzNmmSziZtxlF5E-YkGyIagrj4J4gYFZTWi-Orv8AhzNHnX8hNGW8EJzRD_AjpGFKKpj-PjKBcIiIHuSRBLkmQS0TkQ0TkbS59-fuTz4WnTGT99UlXSauxj8prm84YJoJBQjL2_oj9sqM5_Hd7ud5c5QO5B8O62Iw</recordid><startdate>199810</startdate><enddate>199810</enddate><creator>SOUZA, A. 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R</creatorcontrib><creatorcontrib>GESZTESI, J.-L</creatorcontrib><creatorcontrib>MORAES, J. Z</creatorcontrib><creatorcontrib>CRUZ, C. R. B</creatorcontrib><creatorcontrib>SATO, J</creatorcontrib><creatorcontrib>MARIANO, M</creatorcontrib><creatorcontrib>LOPES, J. 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D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence of idiotypic modulation in the immune response to gp43, the major antigenic component of Paracoccidioides brasiliensis in both mice and humans</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>1998-10</date><risdate>1998</risdate><volume>114</volume><issue>1</issue><spage>40</spage><epage>48</epage><pages>40-48</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><coden>CEXIAL</coden><abstract>Paracoccidioidomycosis (PCM) is a systemic mycosis endemic in Latin America, with a high prevalence in Brazil, Argentina, Colombia and Venezuela. The aetiologic agent of disease is a thermal dimorphic fungus, Paracoccidioides brasiliensis. A glycoprotein of 43 000 D (gp43) is the major antigen of P. brasiliensis. Antibodies directed to this antigen are detected in the sera of all patients with PCM. Gp43 binds to laminin, thus participating in adhesion, invasion and pathogenesis of the fungus. As the role of antibodies in PCM is not fully understood, we decided to investigate the outcome of mice immunization with three distinct anti‐gp43 MoAbs (17c, 8a and 24a) coupled with keyhole limpet haemocyanin (KLH). Results show not only the expected presence of anti‐Id (AB2) antibodies in the sera of these animals but also a spontaneous and increasing amount of anti‐anti‐Id (AB3) antibodies after the third course of immunization. Hybridomas producing both AB2 and AB3 MoAbs were obtained using spleen cells from mice immunized with MoAb 17c. AB3 MoAbs were also obtained with spleen cells of mice immunized with MoAbs 8a and 24a. It was also shown that human PCM patients' sera with high titres of anti‐gp43 antibodies generate anti‐Id antibodies. These data suggest that the immune response to P. brasiliensis can be spontaneously modulated by the idiotypic network.</abstract><cop>Oxford BSL</cop><pub>Blackwell Science Ltd</pub><pmid>9764601</pmid><doi>10.1046/j.1365-2249.1998.00679.x</doi><tpages>9</tpages></addata></record> |
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subjects | Animals Antibodies, Anti-Idiotypic - immunology Antibodies, Fungal - immunology Antibodies, Monoclonal - immunology Antigens, Fungal - immunology Biological and medical sciences Carcinoembryonic Antigen - immunology Female Fungal Proteins General aspects Glycoproteins - immunology gp43 human infection Humans Hybridomas idiotypic network Immunization, Passive Immunoglobulin Idiotypes - blood Immunoglobulin Idiotypes - immunology Medical sciences Mice Mice, Inbred BALB C monoclonal antibodies Oligosaccharides - immunology Original Paracoccidioides - immunology Paracoccidioides brasiliensis Paracoccidioidomycosis - blood Paracoccidioidomycosis - immunology |
title | Evidence of idiotypic modulation in the immune response to gp43, the major antigenic component of Paracoccidioides brasiliensis in both mice and humans |
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