Increased number of T cells committed to IL‐5 production after respiratory syncytial virus (RSV) infection of human mononuclear cells in vitro
We examined changes in the cytokine profile of T cells induced by in vitro infection with RSV. Isolated mononuclear cells from 27 healthy adults and six infants were infected with RSV at a concentration of 3 MOI (multiplicity of infection). After 48 h cells were restimulated with phorbol ester and i...
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| Veröffentlicht in: | Clinical and experimental immunology 1998-09, Vol.113 (3), p.450-455 |
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| creator | THURAU, A. M STRECKERT, H. J RIEGER, C. H. L SCHAUER, U |
| description | We examined changes in the cytokine profile of T cells induced by in vitro infection with RSV. Isolated mononuclear cells from 27 healthy adults and six infants were infected with RSV at a concentration of 3 MOI (multiplicity of infection). After 48 h cells were restimulated with phorbol ester and ionomycin in the presence of monensin for 5 h. The intracellular expression of viral antigen, the cytokines IL‐2, IL‐4, IL‐5, interferon‐gamma (IFN‐γ), and the expression of surface markers were assessed by immunofluorescent staining and flow cytometry. There was a significant (P |
| doi_str_mv | 10.1046/j.1365-2249.1998.00683.x |
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M ; STRECKERT, H. J ; RIEGER, C. H. L ; SCHAUER, U</creator><creatorcontrib>THURAU, A. M ; STRECKERT, H. J ; RIEGER, C. H. L ; SCHAUER, U</creatorcontrib><description>We examined changes in the cytokine profile of T cells induced by in vitro infection with RSV. Isolated mononuclear cells from 27 healthy adults and six infants were infected with RSV at a concentration of 3 MOI (multiplicity of infection). After 48 h cells were restimulated with phorbol ester and ionomycin in the presence of monensin for 5 h. The intracellular expression of viral antigen, the cytokines IL‐2, IL‐4, IL‐5, interferon‐gamma (IFN‐γ), and the expression of surface markers were assessed by immunofluorescent staining and flow cytometry. There was a significant (P < 0.001) rise of IL‐5 expression in RSV‐infected cultures in comparison with uninfected cultures from the same individuals, whereas there were no changes in the expression of the other lymphokines. The increase in IL‐5 generation depended on viable infectious RSV rather than inactivated virus. RSV infection as well as IL‐5 production in T cells were confined to the CD8 subpopulation. However, there was no simultaneous expression of RSV antigen and IL‐5. Purified T cells did not show any increase in IL‐5 generation. However, when the rate of RSV infection was enhanced in monocytes by means of a specific monoclonal antibody, co‐cultured T cells displayed an increase of IL‐5 production compared with samples with ordinary low rate RSV infection. It is therefore likely that the increased commitment of lymphocytes to produce IL‐5 after RSV infection in vitro is mediated by monocytes or other antigen‐presenting cells.</description><identifier>ISSN: 0009-9104</identifier><identifier>EISSN: 1365-2249</identifier><identifier>DOI: 10.1046/j.1365-2249.1998.00683.x</identifier><identifier>PMID: 9737676</identifier><identifier>CODEN: CEXIAL</identifier><language>eng</language><publisher>Oxford BSL: Blackwell Science Ltd</publisher><subject>Adult ; Animals ; Antigen-Presenting Cells - physiology ; antigen‐presenting cell ; Biological and medical sciences ; Human viral diseases ; Humans ; IL‐5 ; Infant ; Infectious diseases ; Interleukin-5 - biosynthesis ; Leukocytes, Mononuclear - virology ; Lymphokines - biosynthesis ; Medical sciences ; Mice ; Original ; respiratory syncytial virus ; Respiratory Syncytial Virus, Human - physiology ; T lymphocyte ; T-Lymphocytes - immunology ; Viral diseases ; Viral diseases of the respiratory system and ent viral diseases</subject><ispartof>Clinical and experimental immunology, 1998-09, Vol.113 (3), p.450-455</ispartof><rights>Blackwell Science Ltd, Oxford</rights><rights>1998 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. 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M</creatorcontrib><creatorcontrib>STRECKERT, H. J</creatorcontrib><creatorcontrib>RIEGER, C. H. L</creatorcontrib><creatorcontrib>SCHAUER, U</creatorcontrib><title>Increased number of T cells committed to IL‐5 production after respiratory syncytial virus (RSV) infection of human mononuclear cells in vitro</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>We examined changes in the cytokine profile of T cells induced by in vitro infection with RSV. Isolated mononuclear cells from 27 healthy adults and six infants were infected with RSV at a concentration of 3 MOI (multiplicity of infection). After 48 h cells were restimulated with phorbol ester and ionomycin in the presence of monensin for 5 h. The intracellular expression of viral antigen, the cytokines IL‐2, IL‐4, IL‐5, interferon‐gamma (IFN‐γ), and the expression of surface markers were assessed by immunofluorescent staining and flow cytometry. There was a significant (P < 0.001) rise of IL‐5 expression in RSV‐infected cultures in comparison with uninfected cultures from the same individuals, whereas there were no changes in the expression of the other lymphokines. The increase in IL‐5 generation depended on viable infectious RSV rather than inactivated virus. RSV infection as well as IL‐5 production in T cells were confined to the CD8 subpopulation. However, there was no simultaneous expression of RSV antigen and IL‐5. Purified T cells did not show any increase in IL‐5 generation. However, when the rate of RSV infection was enhanced in monocytes by means of a specific monoclonal antibody, co‐cultured T cells displayed an increase of IL‐5 production compared with samples with ordinary low rate RSV infection. It is therefore likely that the increased commitment of lymphocytes to produce IL‐5 after RSV infection in vitro is mediated by monocytes or other antigen‐presenting cells.</description><subject>Adult</subject><subject>Animals</subject><subject>Antigen-Presenting Cells - physiology</subject><subject>antigen‐presenting cell</subject><subject>Biological and medical sciences</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>IL‐5</subject><subject>Infant</subject><subject>Infectious diseases</subject><subject>Interleukin-5 - biosynthesis</subject><subject>Leukocytes, Mononuclear - virology</subject><subject>Lymphokines - biosynthesis</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Original</subject><subject>respiratory syncytial virus</subject><subject>Respiratory Syncytial Virus, Human - physiology</subject><subject>T lymphocyte</subject><subject>T-Lymphocytes - immunology</subject><subject>Viral diseases</subject><subject>Viral diseases of the respiratory system and ent viral diseases</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkt-K1DAYxYso67j6CEIQkfWiNWnzF0SQYdWBAUFXb0Oapm6GNplN2t3tnY-wz-iTmDplUG_0Kg3nd86Xr5wsAwgWCGL6alegipK8LLEokBC8gJDyqri9l62Owv1sBSEUuUiOh9mjGHfpSiktT7ITwSpGGV1ldxung1HRNMCNfW0C8C24ANp0XQTa970dhqQNHmy2P77fEbAPvhn1YL0Dqh0SH0zc26AGHyYQJ6enwaoOXNswRnD26fPXl8C61hwcKfty7JUDvXfejbozKiyzrEueIfjH2YNWddE8Wc7T7Mu784v1h3z78f1m_Xaba0JIlfO2IaZFmCOjaoxbpRBuSC0ETdtCzCumqYYlbTGFXEFci7KpBeasQaw2iFSn2ZtD7n6se9No44agOrkPtldhkl5Z-afi7KX85q8lEpBAwlPAiyUg-KvRxEH2Ns67KGf8GCWrRCkqgv8JIgYFp7hM4LO_wJ0fg0t_IQ2lnMMSiQTxA6SDjzGY9vhkBOXcDbmTcwXkXAE5d0P-6oa8Tdanv698NC5lSPrzRVdRq64Nymkbj1hZMS4YTNjrA3ZjOzP993i5Pt-kj-onLz_XuA</recordid><startdate>199809</startdate><enddate>199809</enddate><creator>THURAU, A. M</creator><creator>STRECKERT, H. J</creator><creator>RIEGER, C. H. L</creator><creator>SCHAUER, U</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Oxford University Press</general><general>Blackwell Science Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199809</creationdate><title>Increased number of T cells committed to IL‐5 production after respiratory syncytial virus (RSV) infection of human mononuclear cells in vitro</title><author>THURAU, A. M ; STRECKERT, H. J ; RIEGER, C. H. L ; SCHAUER, U</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5553-8fd5ef1481eab44faa14d5b99610404837c6c026f4608a04b92db9487d17be153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Antigen-Presenting Cells - physiology</topic><topic>antigen‐presenting cell</topic><topic>Biological and medical sciences</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>IL‐5</topic><topic>Infant</topic><topic>Infectious diseases</topic><topic>Interleukin-5 - biosynthesis</topic><topic>Leukocytes, Mononuclear - virology</topic><topic>Lymphokines - biosynthesis</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Original</topic><topic>respiratory syncytial virus</topic><topic>Respiratory Syncytial Virus, Human - physiology</topic><topic>T lymphocyte</topic><topic>T-Lymphocytes - immunology</topic><topic>Viral diseases</topic><topic>Viral diseases of the respiratory system and ent viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>THURAU, A. 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J</au><au>RIEGER, C. H. L</au><au>SCHAUER, U</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased number of T cells committed to IL‐5 production after respiratory syncytial virus (RSV) infection of human mononuclear cells in vitro</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>1998-09</date><risdate>1998</risdate><volume>113</volume><issue>3</issue><spage>450</spage><epage>455</epage><pages>450-455</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><coden>CEXIAL</coden><abstract>We examined changes in the cytokine profile of T cells induced by in vitro infection with RSV. Isolated mononuclear cells from 27 healthy adults and six infants were infected with RSV at a concentration of 3 MOI (multiplicity of infection). After 48 h cells were restimulated with phorbol ester and ionomycin in the presence of monensin for 5 h. The intracellular expression of viral antigen, the cytokines IL‐2, IL‐4, IL‐5, interferon‐gamma (IFN‐γ), and the expression of surface markers were assessed by immunofluorescent staining and flow cytometry. There was a significant (P < 0.001) rise of IL‐5 expression in RSV‐infected cultures in comparison with uninfected cultures from the same individuals, whereas there were no changes in the expression of the other lymphokines. The increase in IL‐5 generation depended on viable infectious RSV rather than inactivated virus. RSV infection as well as IL‐5 production in T cells were confined to the CD8 subpopulation. However, there was no simultaneous expression of RSV antigen and IL‐5. Purified T cells did not show any increase in IL‐5 generation. However, when the rate of RSV infection was enhanced in monocytes by means of a specific monoclonal antibody, co‐cultured T cells displayed an increase of IL‐5 production compared with samples with ordinary low rate RSV infection. It is therefore likely that the increased commitment of lymphocytes to produce IL‐5 after RSV infection in vitro is mediated by monocytes or other antigen‐presenting cells.</abstract><cop>Oxford BSL</cop><pub>Blackwell Science Ltd</pub><pmid>9737676</pmid><doi>10.1046/j.1365-2249.1998.00683.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adult Animals Antigen-Presenting Cells - physiology antigen‐presenting cell Biological and medical sciences Human viral diseases Humans IL‐5 Infant Infectious diseases Interleukin-5 - biosynthesis Leukocytes, Mononuclear - virology Lymphokines - biosynthesis Medical sciences Mice Original respiratory syncytial virus Respiratory Syncytial Virus, Human - physiology T lymphocyte T-Lymphocytes - immunology Viral diseases Viral diseases of the respiratory system and ent viral diseases |
| title | Increased number of T cells committed to IL‐5 production after respiratory syncytial virus (RSV) infection of human mononuclear cells in vitro |
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