Human T cell responses to recombinant mite antigens of Dermatophagoides farinae
We studied T cell responses to four glutathione S transferase (GST)‐fused mite antigens prepared in our laboratory using peripheral blood lymphocytes from mite‐sensitive patients with bronchial asthma. Of the four recombinant antigens, purified GST‐Mag3 had the strongest ability to cause patients�...
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| Veröffentlicht in: | Clinical and experimental immunology 1997-05, Vol.108 (2), p.284-288 |
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| creator | FUJII, S. ONO, K. SHIGETA, S. JYO, T. YAMASHITA, U. |
| description | We studied T cell responses to four glutathione S transferase (GST)‐fused mite antigens prepared in our laboratory using peripheral blood lymphocytes from mite‐sensitive patients with bronchial asthma. Of the four recombinant antigens, purified GST‐Mag3 had the strongest ability to cause patients' lymphocytes to proliferate, and its potency was almost comparable to that of crude mite bodies (Dfb) and faeces (Dff) antigens and a purified major antigen, Der f 2. The responder lymphocytes were mainly T cells, because the proliferative response was depleted by the treatment of lymphocytes with anti‐CD3 antibody and complement, but not with anti‐CD20 antibody and complement. The responsiveness of lymphocytes to GST‐Mag3 correlated with that to Der f 2, but GST‐Mag3 displayed slightly higher activity to stimulate lymphocytes than Der f 2. Simultaneously, the levels of Dff‐ and GST‐Mag3‐specific IgE antibodies correlated with the responsiveness of lymphocytes to GST‐Mag3. These results suggest that Mag3 is a new valuable antigen for the response of T cell proliferation in mite‐sensitive patients. |
| doi_str_mv | 10.1046/j.1365-2249.1997.3431244.x |
| format | Article |
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Of the four recombinant antigens, purified GST‐Mag3 had the strongest ability to cause patients' lymphocytes to proliferate, and its potency was almost comparable to that of crude mite bodies (Dfb) and faeces (Dff) antigens and a purified major antigen, Der f 2. The responder lymphocytes were mainly T cells, because the proliferative response was depleted by the treatment of lymphocytes with anti‐CD3 antibody and complement, but not with anti‐CD20 antibody and complement. The responsiveness of lymphocytes to GST‐Mag3 correlated with that to Der f 2, but GST‐Mag3 displayed slightly higher activity to stimulate lymphocytes than Der f 2. Simultaneously, the levels of Dff‐ and GST‐Mag3‐specific IgE antibodies correlated with the responsiveness of lymphocytes to GST‐Mag3. These results suggest that Mag3 is a new valuable antigen for the response of T cell proliferation in mite‐sensitive patients.</description><identifier>ISSN: 0009-9104</identifier><identifier>EISSN: 1365-2249</identifier><identifier>DOI: 10.1046/j.1365-2249.1997.3431244.x</identifier><identifier>PMID: 9158099</identifier><identifier>CODEN: CEXIAL</identifier><language>eng</language><publisher>Oxford BSL: Blackwell Science Ltd</publisher><subject>Allergens - genetics ; Allergens - immunology ; Allergic diseases ; Animals ; Antibody Specificity ; Antigens, Dermatophagoides ; Asthma - immunology ; Biological and medical sciences ; Dermatophagoides farinae ; Glutathione Transferase - genetics ; Glutathione Transferase - immunology ; Glycoproteins - genetics ; Glycoproteins - immunology ; GST‐fused protein ; Humans ; Immunoglobulin E - metabolism ; Immunoglobulin G - metabolism ; Immunopathology ; Lymphocyte Activation ; Medical sciences ; Mites - genetics ; Mites - immunology ; Original ; recombinant antigen ; Recombinant Fusion Proteins - immunology ; Respiratory and ent allergic diseases ; T cell response ; T-Lymphocytes - immunology</subject><ispartof>Clinical and experimental immunology, 1997-05, Vol.108 (2), p.284-288</ispartof><rights>Blackwell Science Ltd, Oxford</rights><rights>1997 INIST-CNRS</rights><rights>1997 Blackwell Science Ltd 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4683-e226563d59c25a0e6f9ceeb3d290660aa5752492b12bfa2f38e35430aaccacdb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1904645/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1904645/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2664733$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9158099$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FUJII, S.</creatorcontrib><creatorcontrib>ONO, K.</creatorcontrib><creatorcontrib>SHIGETA, S.</creatorcontrib><creatorcontrib>JYO, T.</creatorcontrib><creatorcontrib>YAMASHITA, U.</creatorcontrib><title>Human T cell responses to recombinant mite antigens of Dermatophagoides farinae</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>We studied T cell responses to four glutathione S transferase (GST)‐fused mite antigens prepared in our laboratory using peripheral blood lymphocytes from mite‐sensitive patients with bronchial asthma. Of the four recombinant antigens, purified GST‐Mag3 had the strongest ability to cause patients' lymphocytes to proliferate, and its potency was almost comparable to that of crude mite bodies (Dfb) and faeces (Dff) antigens and a purified major antigen, Der f 2. The responder lymphocytes were mainly T cells, because the proliferative response was depleted by the treatment of lymphocytes with anti‐CD3 antibody and complement, but not with anti‐CD20 antibody and complement. The responsiveness of lymphocytes to GST‐Mag3 correlated with that to Der f 2, but GST‐Mag3 displayed slightly higher activity to stimulate lymphocytes than Der f 2. Simultaneously, the levels of Dff‐ and GST‐Mag3‐specific IgE antibodies correlated with the responsiveness of lymphocytes to GST‐Mag3. These results suggest that Mag3 is a new valuable antigen for the response of T cell proliferation in mite‐sensitive patients.</description><subject>Allergens - genetics</subject><subject>Allergens - immunology</subject><subject>Allergic diseases</subject><subject>Animals</subject><subject>Antibody Specificity</subject><subject>Antigens, Dermatophagoides</subject><subject>Asthma - immunology</subject><subject>Biological and medical sciences</subject><subject>Dermatophagoides farinae</subject><subject>Glutathione Transferase - genetics</subject><subject>Glutathione Transferase - immunology</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - immunology</subject><subject>GST‐fused protein</subject><subject>Humans</subject><subject>Immunoglobulin E - metabolism</subject><subject>Immunoglobulin G - metabolism</subject><subject>Immunopathology</subject><subject>Lymphocyte Activation</subject><subject>Medical sciences</subject><subject>Mites - genetics</subject><subject>Mites - immunology</subject><subject>Original</subject><subject>recombinant antigen</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Respiratory and ent allergic diseases</subject><subject>T cell response</subject><subject>T-Lymphocytes - immunology</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUU1v1DAQtRCoLIWfgBQhxC3BH4l3zQEJLYVWqtRLOVsTZ7L1KrEXOwvtv2eiRgucEL7Yo_eh53mMvRG8ErzW7_eVULoppaxNJYxZV6pWQtZ1df-ErU7QU7binJvSkOY5e5HznkattTxjZ0Y0G27Mit1cHkcIxW3hcBiKhPkQQ8ZcTJEGF8fWBwhTMfoJC3r4HYZcxL74jGmEKR7uYBd9R4IeElHxJXvWw5Dx1XKfs29fLm63l-X1zder7afr0tV6o0qUUjdadY1xsgGOujcOsVWdNBSRAzTrhr4gWyHbHmSvNqiaWhHgHLiuVefs46Pv4diO2DkMU4LBHpIfIT3YCN7-jQR_Z3fxhxWGNlg3ZPBuMUjx-xHzZEef5yVAwHjMdm241IrOv4hCc02eayJ-eCS6FHNO2J_SCG7n3uzezuXYuRw792aX3uw9iV__-Z-TdCmK8LcLDtnB0CcIzucTTWpNCdTvtfz0Az78RwC7vbiiQf0Cgw61bg</recordid><startdate>199705</startdate><enddate>199705</enddate><creator>FUJII, S.</creator><creator>ONO, K.</creator><creator>SHIGETA, S.</creator><creator>JYO, T.</creator><creator>YAMASHITA, U.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Blackwell Science Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199705</creationdate><title>Human T cell responses to recombinant mite antigens of Dermatophagoides farinae</title><author>FUJII, S. ; ONO, K. ; SHIGETA, S. ; JYO, T. ; YAMASHITA, U.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4683-e226563d59c25a0e6f9ceeb3d290660aa5752492b12bfa2f38e35430aaccacdb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Allergens - genetics</topic><topic>Allergens - immunology</topic><topic>Allergic diseases</topic><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Antigens, Dermatophagoides</topic><topic>Asthma - immunology</topic><topic>Biological and medical sciences</topic><topic>Dermatophagoides farinae</topic><topic>Glutathione Transferase - genetics</topic><topic>Glutathione Transferase - immunology</topic><topic>Glycoproteins - genetics</topic><topic>Glycoproteins - immunology</topic><topic>GST‐fused protein</topic><topic>Humans</topic><topic>Immunoglobulin E - metabolism</topic><topic>Immunoglobulin G - metabolism</topic><topic>Immunopathology</topic><topic>Lymphocyte Activation</topic><topic>Medical sciences</topic><topic>Mites - genetics</topic><topic>Mites - immunology</topic><topic>Original</topic><topic>recombinant antigen</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Respiratory and ent allergic diseases</topic><topic>T cell response</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FUJII, S.</creatorcontrib><creatorcontrib>ONO, K.</creatorcontrib><creatorcontrib>SHIGETA, S.</creatorcontrib><creatorcontrib>JYO, T.</creatorcontrib><creatorcontrib>YAMASHITA, U.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical and experimental immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FUJII, S.</au><au>ONO, K.</au><au>SHIGETA, S.</au><au>JYO, T.</au><au>YAMASHITA, U.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human T cell responses to recombinant mite antigens of Dermatophagoides farinae</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>1997-05</date><risdate>1997</risdate><volume>108</volume><issue>2</issue><spage>284</spage><epage>288</epage><pages>284-288</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><coden>CEXIAL</coden><abstract>We studied T cell responses to four glutathione S transferase (GST)‐fused mite antigens prepared in our laboratory using peripheral blood lymphocytes from mite‐sensitive patients with bronchial asthma. Of the four recombinant antigens, purified GST‐Mag3 had the strongest ability to cause patients' lymphocytes to proliferate, and its potency was almost comparable to that of crude mite bodies (Dfb) and faeces (Dff) antigens and a purified major antigen, Der f 2. The responder lymphocytes were mainly T cells, because the proliferative response was depleted by the treatment of lymphocytes with anti‐CD3 antibody and complement, but not with anti‐CD20 antibody and complement. The responsiveness of lymphocytes to GST‐Mag3 correlated with that to Der f 2, but GST‐Mag3 displayed slightly higher activity to stimulate lymphocytes than Der f 2. Simultaneously, the levels of Dff‐ and GST‐Mag3‐specific IgE antibodies correlated with the responsiveness of lymphocytes to GST‐Mag3. These results suggest that Mag3 is a new valuable antigen for the response of T cell proliferation in mite‐sensitive patients.</abstract><cop>Oxford BSL</cop><pub>Blackwell Science Ltd</pub><pmid>9158099</pmid><doi>10.1046/j.1365-2249.1997.3431244.x</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Allergens - genetics Allergens - immunology Allergic diseases Animals Antibody Specificity Antigens, Dermatophagoides Asthma - immunology Biological and medical sciences Dermatophagoides farinae Glutathione Transferase - genetics Glutathione Transferase - immunology Glycoproteins - genetics Glycoproteins - immunology GST‐fused protein Humans Immunoglobulin E - metabolism Immunoglobulin G - metabolism Immunopathology Lymphocyte Activation Medical sciences Mites - genetics Mites - immunology Original recombinant antigen Recombinant Fusion Proteins - immunology Respiratory and ent allergic diseases T cell response T-Lymphocytes - immunology |
| title | Human T cell responses to recombinant mite antigens of Dermatophagoides farinae |
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