Genes misregulated in C. elegans deficient in Dicer, RDE-4, or RDE-1 are enriched for innate immunity genes
We describe the first microarray analysis of a whole animal containing a mutation in the Dicer gene. We used adult Caenorhabditis elegans and, to distinguish among different roles of Dicer, we also performed microarray analyses of animals with mutations in rde-4 and rde-1, which are involved in sile...
Gespeichert in:
| Veröffentlicht in: | RNA (Cambridge) 2007-07, Vol.13 (7), p.1090-1102 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1102 |
|---|---|
| container_issue | 7 |
| container_start_page | 1090 |
| container_title | RNA (Cambridge) |
| container_volume | 13 |
| creator | Welker, Noah C Habig, Jeffrey W Bass, Brenda L |
| description | We describe the first microarray analysis of a whole animal containing a mutation in the Dicer gene. We used adult Caenorhabditis elegans and, to distinguish among different roles of Dicer, we also performed microarray analyses of animals with mutations in rde-4 and rde-1, which are involved in silencing by siRNA, but not miRNA. Surprisingly, we find that the X chromosome is greatly enriched for genes regulated by Dicer. Comparison of all three microarray data sets indicates the majority of Dicer-regulated genes are not dependent on RDE-4 or RDE-1, including the X-linked genes. However, all three data sets are enriched in genes important for innate immunity and, specifically, show increased expression of innate immunity genes. |
| doi_str_mv | 10.1261/rna.542107 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1894926</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20318495</sourcerecordid><originalsourceid>FETCH-LOGICAL-c473t-21027ad93f95f25400b977fe9b0dbb338d960390bc40d016c0399f1c5d047dca3</originalsourceid><addsrcrecordid>eNpVUclKBDEQDaK4jF78AMnJg9hjtl5yEWTGDQRB9BzSSfUY7U5r0i3492acweVUVa9eXlXqIXRIyZSygp4Fr6e5YJSUG2iXikJmkhC6mXKe51nFK7aD9mJ8SSBP7W20Q8ucFYVgu-j1GjxE3LkYYDG2egCLncezKYYWFtpHbKFxxoEflvjcGQin-GF-mYlT3IfvjGIdAIMPzjyn502CnfdJCruuG70bPvFiOWUfbTW6jXCwjhP0dHX5OLvJ7u6vb2cXd5kRJR-y9BFWait5I_OG5YKQWpZlA7Imtq45r6wsCJekNoJYQguTCtlQk1siSms0n6Dzle7bWHdgTdo96Fa9Bdfp8Kl67dT_jnfPatF_KFpJIVmRBI7XAqF_HyEOKt3HQNtqD_0YFSOcVkLmiXiyIprQx3TB5mcIJWrpjUreqJU3iXz0d61f6toM_gXRzol9</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20318495</pqid></control><display><type>article</type><title>Genes misregulated in C. elegans deficient in Dicer, RDE-4, or RDE-1 are enriched for innate immunity genes</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Welker, Noah C ; Habig, Jeffrey W ; Bass, Brenda L</creator><creatorcontrib>Welker, Noah C ; Habig, Jeffrey W ; Bass, Brenda L</creatorcontrib><description>We describe the first microarray analysis of a whole animal containing a mutation in the Dicer gene. We used adult Caenorhabditis elegans and, to distinguish among different roles of Dicer, we also performed microarray analyses of animals with mutations in rde-4 and rde-1, which are involved in silencing by siRNA, but not miRNA. Surprisingly, we find that the X chromosome is greatly enriched for genes regulated by Dicer. Comparison of all three microarray data sets indicates the majority of Dicer-regulated genes are not dependent on RDE-4 or RDE-1, including the X-linked genes. However, all three data sets are enriched in genes important for innate immunity and, specifically, show increased expression of innate immunity genes.</description><identifier>ISSN: 1355-8382</identifier><identifier>EISSN: 1469-9001</identifier><identifier>DOI: 10.1261/rna.542107</identifier><identifier>PMID: 17526642</identifier><language>eng</language><publisher>United States: Cold Spring Harbor Laboratory Press</publisher><subject>Animals ; Animals, Genetically Modified ; Caenorhabditis elegans ; Caenorhabditis elegans - genetics ; Caenorhabditis elegans Proteins - genetics ; Gene Deletion ; Gene Expression Regulation ; Immunity, Innate - genetics ; MicroRNAs - genetics ; Oligonucleotide Array Sequence Analysis ; Ribonuclease III - genetics ; Ribonuclease III - physiology ; RNA, Small Interfering - genetics ; RNA-Binding Proteins - genetics ; X Chromosome - chemistry</subject><ispartof>RNA (Cambridge), 2007-07, Vol.13 (7), p.1090-1102</ispartof><rights>Copyright © 2007 RNA Society 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-21027ad93f95f25400b977fe9b0dbb338d960390bc40d016c0399f1c5d047dca3</citedby><cites>FETCH-LOGICAL-c473t-21027ad93f95f25400b977fe9b0dbb338d960390bc40d016c0399f1c5d047dca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1894926/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1894926/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17526642$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Welker, Noah C</creatorcontrib><creatorcontrib>Habig, Jeffrey W</creatorcontrib><creatorcontrib>Bass, Brenda L</creatorcontrib><title>Genes misregulated in C. elegans deficient in Dicer, RDE-4, or RDE-1 are enriched for innate immunity genes</title><title>RNA (Cambridge)</title><addtitle>RNA</addtitle><description>We describe the first microarray analysis of a whole animal containing a mutation in the Dicer gene. We used adult Caenorhabditis elegans and, to distinguish among different roles of Dicer, we also performed microarray analyses of animals with mutations in rde-4 and rde-1, which are involved in silencing by siRNA, but not miRNA. Surprisingly, we find that the X chromosome is greatly enriched for genes regulated by Dicer. Comparison of all three microarray data sets indicates the majority of Dicer-regulated genes are not dependent on RDE-4 or RDE-1, including the X-linked genes. However, all three data sets are enriched in genes important for innate immunity and, specifically, show increased expression of innate immunity genes.</description><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Caenorhabditis elegans Proteins - genetics</subject><subject>Gene Deletion</subject><subject>Gene Expression Regulation</subject><subject>Immunity, Innate - genetics</subject><subject>MicroRNAs - genetics</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Ribonuclease III - genetics</subject><subject>Ribonuclease III - physiology</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA-Binding Proteins - genetics</subject><subject>X Chromosome - chemistry</subject><issn>1355-8382</issn><issn>1469-9001</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUclKBDEQDaK4jF78AMnJg9hjtl5yEWTGDQRB9BzSSfUY7U5r0i3492acweVUVa9eXlXqIXRIyZSygp4Fr6e5YJSUG2iXikJmkhC6mXKe51nFK7aD9mJ8SSBP7W20Q8ucFYVgu-j1GjxE3LkYYDG2egCLncezKYYWFtpHbKFxxoEflvjcGQin-GF-mYlT3IfvjGIdAIMPzjyn502CnfdJCruuG70bPvFiOWUfbTW6jXCwjhP0dHX5OLvJ7u6vb2cXd5kRJR-y9BFWait5I_OG5YKQWpZlA7Imtq45r6wsCJekNoJYQguTCtlQk1siSms0n6Dzle7bWHdgTdo96Fa9Bdfp8Kl67dT_jnfPatF_KFpJIVmRBI7XAqF_HyEOKt3HQNtqD_0YFSOcVkLmiXiyIprQx3TB5mcIJWrpjUreqJU3iXz0d61f6toM_gXRzol9</recordid><startdate>20070701</startdate><enddate>20070701</enddate><creator>Welker, Noah C</creator><creator>Habig, Jeffrey W</creator><creator>Bass, Brenda L</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20070701</creationdate><title>Genes misregulated in C. elegans deficient in Dicer, RDE-4, or RDE-1 are enriched for innate immunity genes</title><author>Welker, Noah C ; Habig, Jeffrey W ; Bass, Brenda L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-21027ad93f95f25400b977fe9b0dbb338d960390bc40d016c0399f1c5d047dca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Caenorhabditis elegans Proteins - genetics</topic><topic>Gene Deletion</topic><topic>Gene Expression Regulation</topic><topic>Immunity, Innate - genetics</topic><topic>MicroRNAs - genetics</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Ribonuclease III - genetics</topic><topic>Ribonuclease III - physiology</topic><topic>RNA, Small Interfering - genetics</topic><topic>RNA-Binding Proteins - genetics</topic><topic>X Chromosome - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Welker, Noah C</creatorcontrib><creatorcontrib>Habig, Jeffrey W</creatorcontrib><creatorcontrib>Bass, Brenda L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RNA (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Welker, Noah C</au><au>Habig, Jeffrey W</au><au>Bass, Brenda L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genes misregulated in C. elegans deficient in Dicer, RDE-4, or RDE-1 are enriched for innate immunity genes</atitle><jtitle>RNA (Cambridge)</jtitle><addtitle>RNA</addtitle><date>2007-07-01</date><risdate>2007</risdate><volume>13</volume><issue>7</issue><spage>1090</spage><epage>1102</epage><pages>1090-1102</pages><issn>1355-8382</issn><eissn>1469-9001</eissn><abstract>We describe the first microarray analysis of a whole animal containing a mutation in the Dicer gene. We used adult Caenorhabditis elegans and, to distinguish among different roles of Dicer, we also performed microarray analyses of animals with mutations in rde-4 and rde-1, which are involved in silencing by siRNA, but not miRNA. Surprisingly, we find that the X chromosome is greatly enriched for genes regulated by Dicer. Comparison of all three microarray data sets indicates the majority of Dicer-regulated genes are not dependent on RDE-4 or RDE-1, including the X-linked genes. However, all three data sets are enriched in genes important for innate immunity and, specifically, show increased expression of innate immunity genes.</abstract><cop>United States</cop><pub>Cold Spring Harbor Laboratory Press</pub><pmid>17526642</pmid><doi>10.1261/rna.542107</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1355-8382 |
| ispartof | RNA (Cambridge), 2007-07, Vol.13 (7), p.1090-1102 |
| issn | 1355-8382 1469-9001 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1894926 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Animals, Genetically Modified Caenorhabditis elegans Caenorhabditis elegans - genetics Caenorhabditis elegans Proteins - genetics Gene Deletion Gene Expression Regulation Immunity, Innate - genetics MicroRNAs - genetics Oligonucleotide Array Sequence Analysis Ribonuclease III - genetics Ribonuclease III - physiology RNA, Small Interfering - genetics RNA-Binding Proteins - genetics X Chromosome - chemistry |
| title | Genes misregulated in C. elegans deficient in Dicer, RDE-4, or RDE-1 are enriched for innate immunity genes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T17%3A12%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genes%20misregulated%20in%20C.%20elegans%20deficient%20in%20Dicer,%20RDE-4,%20or%20RDE-1%20are%20enriched%20for%20innate%20immunity%20genes&rft.jtitle=RNA%20(Cambridge)&rft.au=Welker,%20Noah%20C&rft.date=2007-07-01&rft.volume=13&rft.issue=7&rft.spage=1090&rft.epage=1102&rft.pages=1090-1102&rft.issn=1355-8382&rft.eissn=1469-9001&rft_id=info:doi/10.1261/rna.542107&rft_dat=%3Cproquest_pubme%3E20318495%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20318495&rft_id=info:pmid/17526642&rfr_iscdi=true |