Heteroduplex DNA in meiotic recombination in Drosophila mei-9 mutants
Meiotic recombination gives rise to crossovers, which are required in most organisms for the faithful segregation of homologous chromosomes during meiotic cell division. Characterization of crossover-defective mutants has contributed much to our understanding of the molecular mechanism of crossover...
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| Veröffentlicht in: | Genetics (Austin) 2007-05, Vol.176 (1), p.63-72 |
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| creator | Radford, Sarah J McMahan, Susan Blanton, Hunter L Sekelsky, Jeff |
| description | Meiotic recombination gives rise to crossovers, which are required in most organisms for the faithful segregation of homologous chromosomes during meiotic cell division. Characterization of crossover-defective mutants has contributed much to our understanding of the molecular mechanism of crossover formation. We report here a molecular analysis of recombination in a Drosophila melanogaster crossover-defective mutant, mei-9. In the absence of mei-9 activity, postmeiotic segregation associated with noncrossovers occurs at the expense of crossover products, suggesting that the underlying meiotic function for MEI-9 is in crossover formation rather than mismatch repair. In support of this, analysis of the arrangement of heteroduplex DNA in the postmeiotic segregation products reveals different patterns from those observed in Drosophila Msh6 mutants, which are mismatch-repair defective. This analysis also provides evidence that the double-strand break repair model applies to meiotic recombination in Drosophila. Our results support a model in which MEI-9 nicks Holliday junctions to generate crossovers during meiotic recombination, and, in the absence of MEI-9 activity, the double Holliday junction intermediate instead undergoes dissolution to generate noncrossover products in which heteroduplex is unrepaired. |
| doi_str_mv | 10.1534/genetics.107.070557 |
| format | Article |
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Characterization of crossover-defective mutants has contributed much to our understanding of the molecular mechanism of crossover formation. We report here a molecular analysis of recombination in a Drosophila melanogaster crossover-defective mutant, mei-9. In the absence of mei-9 activity, postmeiotic segregation associated with noncrossovers occurs at the expense of crossover products, suggesting that the underlying meiotic function for MEI-9 is in crossover formation rather than mismatch repair. In support of this, analysis of the arrangement of heteroduplex DNA in the postmeiotic segregation products reveals different patterns from those observed in Drosophila Msh6 mutants, which are mismatch-repair defective. This analysis also provides evidence that the double-strand break repair model applies to meiotic recombination in Drosophila. Our results support a model in which MEI-9 nicks Holliday junctions to generate crossovers during meiotic recombination, and, in the absence of MEI-9 activity, the double Holliday junction intermediate instead undergoes dissolution to generate noncrossover products in which heteroduplex is unrepaired.</description><identifier>ISSN: 0016-6731</identifier><identifier>ISSN: 1943-2631</identifier><identifier>EISSN: 1943-2631</identifier><identifier>DOI: 10.1534/genetics.107.070557</identifier><identifier>PMID: 17339219</identifier><identifier>CODEN: GENTAE</identifier><language>eng</language><publisher>United States: Genetics Society of America</publisher><subject>Animals ; Base Composition - genetics ; Chromosome Segregation ; Chromosomes ; Crossing Over, Genetic ; Deoxyribonucleic acid ; DNA ; DNA Repair ; Drosophila melanogaster - genetics ; Drosophila Proteins - genetics ; Genetic recombination ; Investigations ; Meiosis ; Models, Genetic ; Mutation - genetics ; Nuclear Proteins - genetics ; Nucleic Acid Heteroduplexes - genetics ; Proteins ; Yeast</subject><ispartof>Genetics (Austin), 2007-05, Vol.176 (1), p.63-72</ispartof><rights>Copyright Genetics Society of America May 2007</rights><rights>Copyright © 2007 by the Genetics Society of America 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-29d5a4b6a999397574319b783b65d56ab541ca65d9859843edbe17c9b323cd323</citedby><cites>FETCH-LOGICAL-c430t-29d5a4b6a999397574319b783b65d56ab541ca65d9859843edbe17c9b323cd323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17339219$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Radford, Sarah J</creatorcontrib><creatorcontrib>McMahan, Susan</creatorcontrib><creatorcontrib>Blanton, Hunter L</creatorcontrib><creatorcontrib>Sekelsky, Jeff</creatorcontrib><title>Heteroduplex DNA in meiotic recombination in Drosophila mei-9 mutants</title><title>Genetics (Austin)</title><addtitle>Genetics</addtitle><description>Meiotic recombination gives rise to crossovers, which are required in most organisms for the faithful segregation of homologous chromosomes during meiotic cell division. Characterization of crossover-defective mutants has contributed much to our understanding of the molecular mechanism of crossover formation. We report here a molecular analysis of recombination in a Drosophila melanogaster crossover-defective mutant, mei-9. In the absence of mei-9 activity, postmeiotic segregation associated with noncrossovers occurs at the expense of crossover products, suggesting that the underlying meiotic function for MEI-9 is in crossover formation rather than mismatch repair. In support of this, analysis of the arrangement of heteroduplex DNA in the postmeiotic segregation products reveals different patterns from those observed in Drosophila Msh6 mutants, which are mismatch-repair defective. This analysis also provides evidence that the double-strand break repair model applies to meiotic recombination in Drosophila. Our results support a model in which MEI-9 nicks Holliday junctions to generate crossovers during meiotic recombination, and, in the absence of MEI-9 activity, the double Holliday junction intermediate instead undergoes dissolution to generate noncrossover products in which heteroduplex is unrepaired.</description><subject>Animals</subject><subject>Base Composition - genetics</subject><subject>Chromosome Segregation</subject><subject>Chromosomes</subject><subject>Crossing Over, Genetic</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Repair</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila Proteins - genetics</subject><subject>Genetic recombination</subject><subject>Investigations</subject><subject>Meiosis</subject><subject>Models, Genetic</subject><subject>Mutation - genetics</subject><subject>Nuclear Proteins - genetics</subject><subject>Nucleic Acid Heteroduplexes - genetics</subject><subject>Proteins</subject><subject>Yeast</subject><issn>0016-6731</issn><issn>1943-2631</issn><issn>1943-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkcFO3DAQhq2qqLuFPgESinroLYsnE9vxBWkFtFRawQXOluMY8CqxFztB8Pb1arctcBmPPN_8mpmfkGOgC2BYnz5Yb0dn0gKoWFBBGROfyBxkjWXFET6TOaXASy4QZuRrSmtKKZes-UJmIBBlBXJOLq_saGPopk1vX4qL62XhfDFYF7JyEa0JQ-u8Hl3w28JFDClsHl2vt0wpi2EatR_TETm4132y3_bvIbn7eXl7flWubn79Pl-uSlMjHctKdkzXLddSSpSCiRpBtqLBlrOOcd2yGozOuWyYbGq0XWtBGNlihabL4ZCc7XQ3UzvYzlg_Rt2rTXSDjq8qaKfeV7x7VA_hWUEjkTKaBX7sBWJ4mmwa1eCSsX2vvQ1TUvmKSDmKDH7_AK7DFH1eTlVQAyKnkCHcQSbfJUV7_28SoGrrkfrrUf4QaudR7jp5u8T_nr0p-AfHwY8M</recordid><startdate>200705</startdate><enddate>200705</enddate><creator>Radford, Sarah J</creator><creator>McMahan, Susan</creator><creator>Blanton, Hunter L</creator><creator>Sekelsky, Jeff</creator><general>Genetics Society of America</general><general>Copyright © 2007 by the Genetics Society of America</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7QP</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200705</creationdate><title>Heteroduplex DNA in meiotic recombination in Drosophila mei-9 mutants</title><author>Radford, Sarah J ; McMahan, Susan ; Blanton, Hunter L ; Sekelsky, Jeff</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-29d5a4b6a999397574319b783b65d56ab541ca65d9859843edbe17c9b323cd323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Base Composition - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genetics (Austin)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Radford, Sarah J</au><au>McMahan, Susan</au><au>Blanton, Hunter L</au><au>Sekelsky, Jeff</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heteroduplex DNA in meiotic recombination in Drosophila mei-9 mutants</atitle><jtitle>Genetics (Austin)</jtitle><addtitle>Genetics</addtitle><date>2007-05</date><risdate>2007</risdate><volume>176</volume><issue>1</issue><spage>63</spage><epage>72</epage><pages>63-72</pages><issn>0016-6731</issn><issn>1943-2631</issn><eissn>1943-2631</eissn><coden>GENTAE</coden><abstract>Meiotic recombination gives rise to crossovers, which are required in most organisms for the faithful segregation of homologous chromosomes during meiotic cell division. Characterization of crossover-defective mutants has contributed much to our understanding of the molecular mechanism of crossover formation. We report here a molecular analysis of recombination in a Drosophila melanogaster crossover-defective mutant, mei-9. In the absence of mei-9 activity, postmeiotic segregation associated with noncrossovers occurs at the expense of crossover products, suggesting that the underlying meiotic function for MEI-9 is in crossover formation rather than mismatch repair. In support of this, analysis of the arrangement of heteroduplex DNA in the postmeiotic segregation products reveals different patterns from those observed in Drosophila Msh6 mutants, which are mismatch-repair defective. This analysis also provides evidence that the double-strand break repair model applies to meiotic recombination in Drosophila. Our results support a model in which MEI-9 nicks Holliday junctions to generate crossovers during meiotic recombination, and, in the absence of MEI-9 activity, the double Holliday junction intermediate instead undergoes dissolution to generate noncrossover products in which heteroduplex is unrepaired.</abstract><cop>United States</cop><pub>Genetics Society of America</pub><pmid>17339219</pmid><doi>10.1534/genetics.107.070557</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Genetics (Austin), 2007-05, Vol.176 (1), p.63-72 |
| issn | 0016-6731 1943-2631 1943-2631 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1893050 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Base Composition - genetics Chromosome Segregation Chromosomes Crossing Over, Genetic Deoxyribonucleic acid DNA DNA Repair Drosophila melanogaster - genetics Drosophila Proteins - genetics Genetic recombination Investigations Meiosis Models, Genetic Mutation - genetics Nuclear Proteins - genetics Nucleic Acid Heteroduplexes - genetics Proteins Yeast |
| title | Heteroduplex DNA in meiotic recombination in Drosophila mei-9 mutants |
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