Modulation of multiple neutrophil functions by preparative methods or trace concentrations of bacterial lipopolysaccharide

Human neutrophils were isolated from peripheral blood by four methods: 1) Ficoll-Hypaque gradients and erythrocyte lysis, 2) plasma-Percoll gradients, 3) a "lipopolysaccharide (LPS)-free" method yielding 85% neutrophils, and 4) by centrifugation of cells prepared by Method 3 through a plas...

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Veröffentlicht in:	The American journal of pathology 1985-04, Vol.119 (1), p.101-110
Hauptverfasser:	Haslett, C, Guthrie, LA, Kopaniak, MM, Johnston, RB, Jr, Henson, PM
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium Chloride - pharmacology Biological and medical sciences Chemotaxis, Leukocyte - drug effects Ficoll Humans Immunological methods for diagnosis and exploration Immunopathology Lipopolysaccharides - pharmacology Medical sciences Neutrophils - drug effects Neutrophils - enzymology Neutrophils - physiology Neutrophils - ultrastructure Other methods Superoxides - metabolism
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