Oncodevelopmental expression of rat placental alkaline phosphatase. Detection in oval cells during liver carcinogenesis
Oval cells isolated from livers of rats fed a choline-deficient diet containing 0.1% DL-ethionine (CDE) have an alkaline phosphatase (ALKP) isozyme which can be distinguished by its electrophoretic mobility from the enzyme present in parenchymal cells isolated from normal liver or livers of rats fed...
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| Veröffentlicht in: | The American journal of pathology 1985-12, Vol.121 (3), p.505-513 |
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| creator | Yaswen, P Thompson, NL Fausto, N |
| description | Oval cells isolated from livers of rats fed a choline-deficient diet containing 0.1% DL-ethionine (CDE) have an alkaline phosphatase (ALKP) isozyme which can be distinguished by its electrophoretic mobility from the enzyme present in parenchymal cells isolated from normal liver or livers of rats fed the CDE diet for 4 weeks. The oval cell ALKP has the same electrophoretic mobility as the enzyme from fetal rat liver and placenta. ALKPs from oval cells, parenchymal cells, and placenta all differ from the intestinal enzyme by their electrophoretic mobility, isoelectric focusing, and the patterns of amino acid inhibition of enzyme activity. Oval cells in preneoplastic livers, fetal hepatocytes, and tumor cells of a primary hepatocellular carcinoma induced by CDE feeding stained with a monoclonal antibody directed against rat placental ALKP. Hepatocytes (in normal or preneoplastic livers) and bile duct cells in normal liver did not stain with the same antibody. Placental ALKP may thus be a useful marker in tracing the origin and fate of oval cells during hepatocarcinogenesis. |
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Detection in oval cells during liver carcinogenesis</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Yaswen, P ; Thompson, NL ; Fausto, N</creator><creatorcontrib>Yaswen, P ; Thompson, NL ; Fausto, N</creatorcontrib><description>Oval cells isolated from livers of rats fed a choline-deficient diet containing 0.1% DL-ethionine (CDE) have an alkaline phosphatase (ALKP) isozyme which can be distinguished by its electrophoretic mobility from the enzyme present in parenchymal cells isolated from normal liver or livers of rats fed the CDE diet for 4 weeks. The oval cell ALKP has the same electrophoretic mobility as the enzyme from fetal rat liver and placenta. ALKPs from oval cells, parenchymal cells, and placenta all differ from the intestinal enzyme by their electrophoretic mobility, isoelectric focusing, and the patterns of amino acid inhibition of enzyme activity. Oval cells in preneoplastic livers, fetal hepatocytes, and tumor cells of a primary hepatocellular carcinoma induced by CDE feeding stained with a monoclonal antibody directed against rat placental ALKP. Hepatocytes (in normal or preneoplastic livers) and bile duct cells in normal liver did not stain with the same antibody. Placental ALKP may thus be a useful marker in tracing the origin and fate of oval cells during hepatocarcinogenesis.</description><identifier>ISSN: 0002-9440</identifier><identifier>EISSN: 1525-2191</identifier><identifier>PMID: 3907368</identifier><language>eng</language><publisher>United States: ASIP</publisher><subject>Alkaline Phosphatase - analysis ; Alkaline Phosphatase - antagonists & inhibitors ; Amino Acids - pharmacology ; Animals ; Antibodies, Monoclonal ; Clinical Enzyme Tests ; Electrophoresis, Polyacrylamide Gel ; Female ; Immunoenzyme Techniques ; Isoenzymes - analysis ; Isoenzymes - antagonists & inhibitors ; Liver - enzymology ; Liver Neoplasms, Experimental - diagnosis ; Male ; Neuraminidase - pharmacology ; Placenta - enzymology ; Pregnancy ; Rats ; Rats, Inbred Strains ; Tissue Distribution</subject><ispartof>The American journal of pathology, 1985-12, Vol.121 (3), p.505-513</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1887918/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1887918/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3907368$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yaswen, P</creatorcontrib><creatorcontrib>Thompson, NL</creatorcontrib><creatorcontrib>Fausto, N</creatorcontrib><title>Oncodevelopmental expression of rat placental alkaline phosphatase. Detection in oval cells during liver carcinogenesis</title><title>The American journal of pathology</title><addtitle>Am J Pathol</addtitle><description>Oval cells isolated from livers of rats fed a choline-deficient diet containing 0.1% DL-ethionine (CDE) have an alkaline phosphatase (ALKP) isozyme which can be distinguished by its electrophoretic mobility from the enzyme present in parenchymal cells isolated from normal liver or livers of rats fed the CDE diet for 4 weeks. The oval cell ALKP has the same electrophoretic mobility as the enzyme from fetal rat liver and placenta. ALKPs from oval cells, parenchymal cells, and placenta all differ from the intestinal enzyme by their electrophoretic mobility, isoelectric focusing, and the patterns of amino acid inhibition of enzyme activity. Oval cells in preneoplastic livers, fetal hepatocytes, and tumor cells of a primary hepatocellular carcinoma induced by CDE feeding stained with a monoclonal antibody directed against rat placental ALKP. Hepatocytes (in normal or preneoplastic livers) and bile duct cells in normal liver did not stain with the same antibody. Placental ALKP may thus be a useful marker in tracing the origin and fate of oval cells during hepatocarcinogenesis.</description><subject>Alkaline Phosphatase - analysis</subject><subject>Alkaline Phosphatase - antagonists & inhibitors</subject><subject>Amino Acids - pharmacology</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Clinical Enzyme Tests</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>Immunoenzyme Techniques</subject><subject>Isoenzymes - analysis</subject><subject>Isoenzymes - antagonists & inhibitors</subject><subject>Liver - enzymology</subject><subject>Liver Neoplasms, Experimental - diagnosis</subject><subject>Male</subject><subject>Neuraminidase - pharmacology</subject><subject>Placenta - enzymology</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Tissue Distribution</subject><issn>0002-9440</issn><issn>1525-2191</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE1Lw0AQhhdRaq3-BGFP3iL7ka-9CFI_odCLnpfJZpJs3SZhN436701pET0Nw_M-L8yckDlPRBIJrvgpmTPGRKTimJ2TixA205rKnM3ITCqWyTSfk891a7oSR3Rdv8V2AEfxq_cYgu1a2lXUw0B7B-bAwH2Asy3SvulC38AAAW_pAw5ohr1gJ2eccgadC7TcedvW1NkRPTXgjW27GlsMNlySswpcwKvjXJD3p8e35Uu0Wj-_Lu9XUSNEPEQVk2WhIFeiKFRWQMV4maKSJo6rqjQcRQ4cZIZGGWlMViSxLBMmkzRO81IJuSB3h95-V2yx3J_hwene2y34b92B1f9Jaxtdd6PmeZ4pnk8F138Lfs3jByd-c-CNrZtP61GHLTg3pbmGTc8F11InLJE_Ml2AJw</recordid><startdate>19851201</startdate><enddate>19851201</enddate><creator>Yaswen, P</creator><creator>Thompson, NL</creator><creator>Fausto, N</creator><general>ASIP</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>5PM</scope></search><sort><creationdate>19851201</creationdate><title>Oncodevelopmental expression of rat placental alkaline phosphatase. Detection in oval cells during liver carcinogenesis</title><author>Yaswen, P ; Thompson, NL ; Fausto, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h224t-f03db9a892bb97baf01d6e93c44ffdc1e28a1a37ec9c3cc7b543d50356468d923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Alkaline Phosphatase - analysis</topic><topic>Alkaline Phosphatase - antagonists & inhibitors</topic><topic>Amino Acids - pharmacology</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Clinical Enzyme Tests</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>Immunoenzyme Techniques</topic><topic>Isoenzymes - analysis</topic><topic>Isoenzymes - antagonists & inhibitors</topic><topic>Liver - enzymology</topic><topic>Liver Neoplasms, Experimental - diagnosis</topic><topic>Male</topic><topic>Neuraminidase - pharmacology</topic><topic>Placenta - enzymology</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yaswen, P</creatorcontrib><creatorcontrib>Thompson, NL</creatorcontrib><creatorcontrib>Fausto, N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The American journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yaswen, P</au><au>Thompson, NL</au><au>Fausto, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oncodevelopmental expression of rat placental alkaline phosphatase. Detection in oval cells during liver carcinogenesis</atitle><jtitle>The American journal of pathology</jtitle><addtitle>Am J Pathol</addtitle><date>1985-12-01</date><risdate>1985</risdate><volume>121</volume><issue>3</issue><spage>505</spage><epage>513</epage><pages>505-513</pages><issn>0002-9440</issn><eissn>1525-2191</eissn><abstract>Oval cells isolated from livers of rats fed a choline-deficient diet containing 0.1% DL-ethionine (CDE) have an alkaline phosphatase (ALKP) isozyme which can be distinguished by its electrophoretic mobility from the enzyme present in parenchymal cells isolated from normal liver or livers of rats fed the CDE diet for 4 weeks. The oval cell ALKP has the same electrophoretic mobility as the enzyme from fetal rat liver and placenta. ALKPs from oval cells, parenchymal cells, and placenta all differ from the intestinal enzyme by their electrophoretic mobility, isoelectric focusing, and the patterns of amino acid inhibition of enzyme activity. Oval cells in preneoplastic livers, fetal hepatocytes, and tumor cells of a primary hepatocellular carcinoma induced by CDE feeding stained with a monoclonal antibody directed against rat placental ALKP. Hepatocytes (in normal or preneoplastic livers) and bile duct cells in normal liver did not stain with the same antibody. Placental ALKP may thus be a useful marker in tracing the origin and fate of oval cells during hepatocarcinogenesis.</abstract><cop>United States</cop><pub>ASIP</pub><pmid>3907368</pmid><tpages>9</tpages></addata></record> |
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| ispartof | The American journal of pathology, 1985-12, Vol.121 (3), p.505-513 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Alkaline Phosphatase - analysis Alkaline Phosphatase - antagonists & inhibitors Amino Acids - pharmacology Animals Antibodies, Monoclonal Clinical Enzyme Tests Electrophoresis, Polyacrylamide Gel Female Immunoenzyme Techniques Isoenzymes - analysis Isoenzymes - antagonists & inhibitors Liver - enzymology Liver Neoplasms, Experimental - diagnosis Male Neuraminidase - pharmacology Placenta - enzymology Pregnancy Rats Rats, Inbred Strains Tissue Distribution |
| title | Oncodevelopmental expression of rat placental alkaline phosphatase. Detection in oval cells during liver carcinogenesis |
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