Differential expression of SKALP/Elafin in human epidermal tumors
Recently we described a new epidermal serine proteinase inhibitor, skin-derived antileukoproteinase (SKALP), also known as elafin. SKALP/elafin was found to be absent in normal human epidermis, but can be induced in vitro and in vivo under hyperproliferative conditions. Here we studied the expressio...
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| Veröffentlicht in: | The American journal of pathology 1993-12, Vol.143 (6), p.1679-1687 |
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| creator | Alkemade, HA Molhuizen, HO van Vlijmen-Willems, IM van Haelst, UJ Schalkwijk, J |
| description | Recently we described a new epidermal serine proteinase inhibitor, skin-derived antileukoproteinase (SKALP), also known as elafin. SKALP/elafin was found to be absent in normal human epidermis, but can be induced in vitro and in vivo under hyperproliferative conditions. Here we studied the expression of SKALP/elafin in several types of epidermal tumors (basal cell carcinoma, squamous cell carcinoma, Bowen's disease, actinic keratosis, and keratoacanthoma). Using immunohistochemical staining SKALP/elafin appeared to be differentially expressed in these tumors. Functional measurements of anti-proteinase activity, and Western blotting of tumor extracts confirmed our findings at the histological level. In well differentiated squamous cell carcinoma, SKALP/elafin messenger RNA was demonstrated by non-radioactive in situ hybridization. We conclude that SKALP/elafin is a marker for abnormal or disturbed squamous differentiation. A possible role of SKALP/elafin in the control of tumor cell invasion is discussed. |
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SKALP/elafin was found to be absent in normal human epidermis, but can be induced in vitro and in vivo under hyperproliferative conditions. Here we studied the expression of SKALP/elafin in several types of epidermal tumors (basal cell carcinoma, squamous cell carcinoma, Bowen's disease, actinic keratosis, and keratoacanthoma). Using immunohistochemical staining SKALP/elafin appeared to be differentially expressed in these tumors. Functional measurements of anti-proteinase activity, and Western blotting of tumor extracts confirmed our findings at the histological level. In well differentiated squamous cell carcinoma, SKALP/elafin messenger RNA was demonstrated by non-radioactive in situ hybridization. We conclude that SKALP/elafin is a marker for abnormal or disturbed squamous differentiation. A possible role of SKALP/elafin in the control of tumor cell invasion is discussed.</description><identifier>ISSN: 0002-9440</identifier><identifier>EISSN: 1525-2191</identifier><identifier>PMID: 8256855</identifier><identifier>CODEN: AJPAA4</identifier><language>eng</language><publisher>Bethesda, MD: ASIP</publisher><subject>Biological and medical sciences ; Blotting, Western ; Bowen's Disease - chemistry ; Bowen's Disease - pathology ; Carcinoma, Basal Cell - chemistry ; Carcinoma, Basal Cell - pathology ; Carcinoma, Basal Cell - physiopathology ; Carcinoma, Squamous Cell - chemistry ; Carcinoma, Squamous Cell - pathology ; Carcinoma, Squamous Cell - physiopathology ; Dermatology ; Electrophoresis, Polyacrylamide Gel ; Humans ; Immunohistochemistry ; In Situ Hybridization ; Keratoacanthoma - metabolism ; Keratoacanthoma - pathology ; Medical sciences ; Proteinase Inhibitory Proteins, Secretory ; Proteins ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; Serine Proteinase Inhibitors - analysis ; Serine Proteinase Inhibitors - genetics ; Serine Proteinase Inhibitors - metabolism ; Skin - chemistry ; Skin - metabolism ; Skin - pathology ; Skin Diseases - metabolism ; Skin Diseases - pathology ; Skin Neoplasms - chemistry ; Skin Neoplasms - pathology ; Skin Neoplasms - physiopathology ; Tumors of the skin and soft tissue. Premalignant lesions</subject><ispartof>The American journal of pathology, 1993-12, Vol.143 (6), p.1679-1687</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1887253/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1887253/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3900555$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8256855$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alkemade, HA</creatorcontrib><creatorcontrib>Molhuizen, HO</creatorcontrib><creatorcontrib>van Vlijmen-Willems, IM</creatorcontrib><creatorcontrib>van Haelst, UJ</creatorcontrib><creatorcontrib>Schalkwijk, J</creatorcontrib><title>Differential expression of SKALP/Elafin in human epidermal tumors</title><title>The American journal of pathology</title><addtitle>Am J Pathol</addtitle><description>Recently we described a new epidermal serine proteinase inhibitor, skin-derived antileukoproteinase (SKALP), also known as elafin. SKALP/elafin was found to be absent in normal human epidermis, but can be induced in vitro and in vivo under hyperproliferative conditions. Here we studied the expression of SKALP/elafin in several types of epidermal tumors (basal cell carcinoma, squamous cell carcinoma, Bowen's disease, actinic keratosis, and keratoacanthoma). Using immunohistochemical staining SKALP/elafin appeared to be differentially expressed in these tumors. Functional measurements of anti-proteinase activity, and Western blotting of tumor extracts confirmed our findings at the histological level. In well differentiated squamous cell carcinoma, SKALP/elafin messenger RNA was demonstrated by non-radioactive in situ hybridization. We conclude that SKALP/elafin is a marker for abnormal or disturbed squamous differentiation. A possible role of SKALP/elafin in the control of tumor cell invasion is discussed.</description><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Bowen's Disease - chemistry</subject><subject>Bowen's Disease - pathology</subject><subject>Carcinoma, Basal Cell - chemistry</subject><subject>Carcinoma, Basal Cell - pathology</subject><subject>Carcinoma, Basal Cell - physiopathology</subject><subject>Carcinoma, Squamous Cell - chemistry</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Carcinoma, Squamous Cell - physiopathology</subject><subject>Dermatology</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Keratoacanthoma - metabolism</subject><subject>Keratoacanthoma - pathology</subject><subject>Medical sciences</subject><subject>Proteinase Inhibitory Proteins, Secretory</subject><subject>Proteins</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>Serine Proteinase Inhibitors - analysis</subject><subject>Serine Proteinase Inhibitors - genetics</subject><subject>Serine Proteinase Inhibitors - metabolism</subject><subject>Skin - chemistry</subject><subject>Skin - metabolism</subject><subject>Skin - pathology</subject><subject>Skin Diseases - metabolism</subject><subject>Skin Diseases - pathology</subject><subject>Skin Neoplasms - chemistry</subject><subject>Skin Neoplasms - pathology</subject><subject>Skin Neoplasms - physiopathology</subject><subject>Tumors of the skin and soft tissue. Premalignant lesions</subject><issn>0002-9440</issn><issn>1525-2191</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkFlLw0AUhYMotS4_QciD6FNw1szkRSi1LlhQUJ-H22SmmZLNmcTl3zvYUBQuXC7n45zL2YummBOeEJzh_WiKECJJxhg6jI6834QzpRJNookkPJWcT6PZjTVGO930FqpYf3VOe2_bJm5N_PI4Wz5fLSowtonDlEMNTaw7W2hXB7of6tb5k-jAQOX16biPo7fbxev8Plk-3T3MZ8ukpIT0CQiS0ZQBwZinQmPIGayKggFiBosVw-mKa4CCSm2YMJJkosiokAUUGHAO9Di63vp2w6rWRR5edlCpztka3Ldqwar_SmNLtW4_FJZSEE6DwcVo4Nr3Qfte1dbnuqqg0e3glUhRhrCQATz7m7SLGEsL-vmog8-hMg6a3PodRjOE-C92ucVKuy4_rdPKh9aqYIoVbDrMqEoVTkVGfwAuZ4SM</recordid><startdate>19931201</startdate><enddate>19931201</enddate><creator>Alkemade, HA</creator><creator>Molhuizen, HO</creator><creator>van Vlijmen-Willems, IM</creator><creator>van Haelst, UJ</creator><creator>Schalkwijk, J</creator><general>ASIP</general><general>American Society for Investigative Pathology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19931201</creationdate><title>Differential expression of SKALP/Elafin in human epidermal tumors</title><author>Alkemade, HA ; Molhuizen, HO ; van Vlijmen-Willems, IM ; van Haelst, UJ ; Schalkwijk, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h322t-a729364a211567e1ac4abdd4a04f17b416b5eaad38ef47f8297d9378dad1a1ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Bowen's Disease - chemistry</topic><topic>Bowen's Disease - pathology</topic><topic>Carcinoma, Basal Cell - chemistry</topic><topic>Carcinoma, Basal Cell - pathology</topic><topic>Carcinoma, Basal Cell - physiopathology</topic><topic>Carcinoma, Squamous Cell - chemistry</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Carcinoma, Squamous Cell - physiopathology</topic><topic>Dermatology</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Keratoacanthoma - metabolism</topic><topic>Keratoacanthoma - pathology</topic><topic>Medical sciences</topic><topic>Proteinase Inhibitory Proteins, Secretory</topic><topic>Proteins</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>Serine Proteinase Inhibitors - analysis</topic><topic>Serine Proteinase Inhibitors - genetics</topic><topic>Serine Proteinase Inhibitors - metabolism</topic><topic>Skin - chemistry</topic><topic>Skin - metabolism</topic><topic>Skin - pathology</topic><topic>Skin Diseases - metabolism</topic><topic>Skin Diseases - pathology</topic><topic>Skin Neoplasms - chemistry</topic><topic>Skin Neoplasms - pathology</topic><topic>Skin Neoplasms - physiopathology</topic><topic>Tumors of the skin and soft tissue. Premalignant lesions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alkemade, HA</creatorcontrib><creatorcontrib>Molhuizen, HO</creatorcontrib><creatorcontrib>van Vlijmen-Willems, IM</creatorcontrib><creatorcontrib>van Haelst, UJ</creatorcontrib><creatorcontrib>Schalkwijk, J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The American journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alkemade, HA</au><au>Molhuizen, HO</au><au>van Vlijmen-Willems, IM</au><au>van Haelst, UJ</au><au>Schalkwijk, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential expression of SKALP/Elafin in human epidermal tumors</atitle><jtitle>The American journal of pathology</jtitle><addtitle>Am J Pathol</addtitle><date>1993-12-01</date><risdate>1993</risdate><volume>143</volume><issue>6</issue><spage>1679</spage><epage>1687</epage><pages>1679-1687</pages><issn>0002-9440</issn><eissn>1525-2191</eissn><coden>AJPAA4</coden><abstract>Recently we described a new epidermal serine proteinase inhibitor, skin-derived antileukoproteinase (SKALP), also known as elafin. SKALP/elafin was found to be absent in normal human epidermis, but can be induced in vitro and in vivo under hyperproliferative conditions. Here we studied the expression of SKALP/elafin in several types of epidermal tumors (basal cell carcinoma, squamous cell carcinoma, Bowen's disease, actinic keratosis, and keratoacanthoma). Using immunohistochemical staining SKALP/elafin appeared to be differentially expressed in these tumors. Functional measurements of anti-proteinase activity, and Western blotting of tumor extracts confirmed our findings at the histological level. In well differentiated squamous cell carcinoma, SKALP/elafin messenger RNA was demonstrated by non-radioactive in situ hybridization. We conclude that SKALP/elafin is a marker for abnormal or disturbed squamous differentiation. A possible role of SKALP/elafin in the control of tumor cell invasion is discussed.</abstract><cop>Bethesda, MD</cop><pub>ASIP</pub><pmid>8256855</pmid><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Biological and medical sciences Blotting, Western Bowen's Disease - chemistry Bowen's Disease - pathology Carcinoma, Basal Cell - chemistry Carcinoma, Basal Cell - pathology Carcinoma, Basal Cell - physiopathology Carcinoma, Squamous Cell - chemistry Carcinoma, Squamous Cell - pathology Carcinoma, Squamous Cell - physiopathology Dermatology Electrophoresis, Polyacrylamide Gel Humans Immunohistochemistry In Situ Hybridization Keratoacanthoma - metabolism Keratoacanthoma - pathology Medical sciences Proteinase Inhibitory Proteins, Secretory Proteins RNA, Messenger - analysis RNA, Messenger - genetics Serine Proteinase Inhibitors - analysis Serine Proteinase Inhibitors - genetics Serine Proteinase Inhibitors - metabolism Skin - chemistry Skin - metabolism Skin - pathology Skin Diseases - metabolism Skin Diseases - pathology Skin Neoplasms - chemistry Skin Neoplasms - pathology Skin Neoplasms - physiopathology Tumors of the skin and soft tissue. Premalignant lesions |
| title | Differential expression of SKALP/Elafin in human epidermal tumors |
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