Phenotypic and genotypic analysis of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc
D341 Med is a new continuous cell line and transplantable xenograft derived from a cerebellar medulloblastoma. This line grew in vitro in suspension culture with spontaneous macroscopic spheroid formation and demonstrated 20-fold amplification of c-myc. Cultured D341 Med cells injected subcutaneousl...
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| Veröffentlicht in: | The American journal of pathology 1988-03, Vol.130 (3), p.472-484 |
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| container_title | The American journal of pathology |
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| creator | Friedman, HS Burger, PC Bigner, SH Trojanowski, JQ Brodeur, GM He, XM Wikstrand, CJ Kurtzberg, J Berens, ME Halperin, EC |
| description | D341 Med is a new continuous cell line and transplantable xenograft derived from a cerebellar medulloblastoma. This line grew in vitro in suspension culture with spontaneous macroscopic spheroid formation and demonstrated 20-fold amplification of c-myc. Cultured D341 Med cells injected subcutaneously into athymic mice grew as markedly cellular, highly invasive undifferentiated neoplasms. Intracranial tumors grew as markedly cellular mitotically active neoplasms largely located within the subarachnoid space or lining the ventricular system. Immunocytochemical analysis of the cell line and SQ tumors revealed the high (NFP-H) and middle (NFP-M) molecular weight (Mr) neurofilament proteins (NFPs). Immunoblots demonstrated the presence of molecular species that co-migrated with authentic human NFP-H and NFP-M. This cell line and transplantable xenograft may allow, in conjunction with the authors' other models of human medulloblastoma, analysis of the heterogeneous biologic properties and therapeutic sensitivity of this tumor. |
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This line grew in vitro in suspension culture with spontaneous macroscopic spheroid formation and demonstrated 20-fold amplification of c-myc. Cultured D341 Med cells injected subcutaneously into athymic mice grew as markedly cellular, highly invasive undifferentiated neoplasms. Intracranial tumors grew as markedly cellular mitotically active neoplasms largely located within the subarachnoid space or lining the ventricular system. Immunocytochemical analysis of the cell line and SQ tumors revealed the high (NFP-H) and middle (NFP-M) molecular weight (Mr) neurofilament proteins (NFPs). Immunoblots demonstrated the presence of molecular species that co-migrated with authentic human NFP-H and NFP-M. This cell line and transplantable xenograft may allow, in conjunction with the authors' other models of human medulloblastoma, analysis of the heterogeneous biologic properties and therapeutic sensitivity of this tumor.</description><identifier>ISSN: 0002-9440</identifier><identifier>EISSN: 1525-2191</identifier><identifier>PMID: 3279793</identifier><identifier>CODEN: AJPAA4</identifier><language>eng</language><publisher>Bethesda, MD: ASIP</publisher><subject>Animals ; Biological and medical sciences ; Cell Line ; Cerebellar Neoplasms - genetics ; Cerebellar Neoplasms - pathology ; Child, Preschool ; Female ; Gene Amplification ; Genotype ; Humans ; Immunosorbent Techniques ; Karyotyping ; Male ; Medical sciences ; Medulloblastoma - genetics ; Medulloblastoma - pathology ; Mice ; Mice, Nude ; Microscopy, Electron ; Neoplasm Transplantation ; Neurology ; Oncogenes ; Phenotype ; Radioimmunoassay ; Transplantation, Heterologous ; Tumors of the nervous system. Phacomatoses</subject><ispartof>The American journal of pathology, 1988-03, Vol.130 (3), p.472-484</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1880676/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1880676/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7245541$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3279793$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Friedman, HS</creatorcontrib><creatorcontrib>Burger, PC</creatorcontrib><creatorcontrib>Bigner, SH</creatorcontrib><creatorcontrib>Trojanowski, JQ</creatorcontrib><creatorcontrib>Brodeur, GM</creatorcontrib><creatorcontrib>He, XM</creatorcontrib><creatorcontrib>Wikstrand, CJ</creatorcontrib><creatorcontrib>Kurtzberg, J</creatorcontrib><creatorcontrib>Berens, ME</creatorcontrib><creatorcontrib>Halperin, EC</creatorcontrib><title>Phenotypic and genotypic analysis of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc</title><title>The American journal of pathology</title><addtitle>Am J Pathol</addtitle><description>D341 Med is a new continuous cell line and transplantable xenograft derived from a cerebellar medulloblastoma. This line grew in vitro in suspension culture with spontaneous macroscopic spheroid formation and demonstrated 20-fold amplification of c-myc. Cultured D341 Med cells injected subcutaneously into athymic mice grew as markedly cellular, highly invasive undifferentiated neoplasms. Intracranial tumors grew as markedly cellular mitotically active neoplasms largely located within the subarachnoid space or lining the ventricular system. Immunocytochemical analysis of the cell line and SQ tumors revealed the high (NFP-H) and middle (NFP-M) molecular weight (Mr) neurofilament proteins (NFPs). Immunoblots demonstrated the presence of molecular species that co-migrated with authentic human NFP-H and NFP-M. This cell line and transplantable xenograft may allow, in conjunction with the authors' other models of human medulloblastoma, analysis of the heterogeneous biologic properties and therapeutic sensitivity of this tumor.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cerebellar Neoplasms - genetics</subject><subject>Cerebellar Neoplasms - pathology</subject><subject>Child, Preschool</subject><subject>Female</subject><subject>Gene Amplification</subject><subject>Genotype</subject><subject>Humans</subject><subject>Immunosorbent Techniques</subject><subject>Karyotyping</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Medulloblastoma - genetics</subject><subject>Medulloblastoma - pathology</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Microscopy, Electron</subject><subject>Neoplasm Transplantation</subject><subject>Neurology</subject><subject>Oncogenes</subject><subject>Phenotype</subject><subject>Radioimmunoassay</subject><subject>Transplantation, Heterologous</subject><subject>Tumors of the nervous system. Phacomatoses</subject><issn>0002-9440</issn><issn>1525-2191</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2L1jAUhYso4-voTxCyENFFIV9tko0g4yeM6ELX4TZN2gz5qE2rvr_DP2xGX8Zx5SoczslzT27uNAfS0a6lRJG7zQFjTFvFOb7fPCjlqsqeSXzWnDEqlFDs0Pz8NNuUt-PiDYI0oumWgnAsvqDsEKB5j5BQtOMeQh4ClC1HQMaGgIJP9vfdbYVUlgBpgyFY9KOiphXchp69YpygD3Z8jkYbcyo1ufk0IYhL8M6bqnK6HmTaeDQPm3sOQrGPTud58-XN688X79rLj2_fX7y8bGfW0a0VciTYGcyccwL3ymBlnHAwKExHKx3puQSnht5SUJxh7saOGEa4HJyV3LHz5sUf7rIP9WXGptor6GX1EdajzuD1v07ys57yN02kxL3oK-DpCbDmr7stm46-XO8Eks170UISqrDE_w2SjjAiOa_Bx7cr3XQ5_Vf1n5x8KAaCqxs3vtzEBOVdx8nfebOf5u9-tbpECKFCiYarhTCsmeaCsl9SZbBK</recordid><startdate>19880301</startdate><enddate>19880301</enddate><creator>Friedman, HS</creator><creator>Burger, PC</creator><creator>Bigner, SH</creator><creator>Trojanowski, JQ</creator><creator>Brodeur, GM</creator><creator>He, XM</creator><creator>Wikstrand, CJ</creator><creator>Kurtzberg, J</creator><creator>Berens, ME</creator><creator>Halperin, EC</creator><general>ASIP</general><general>American Society for Investigative Pathology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19880301</creationdate><title>Phenotypic and genotypic analysis of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc</title><author>Friedman, HS ; Burger, PC ; Bigner, SH ; Trojanowski, JQ ; Brodeur, GM ; He, XM ; Wikstrand, CJ ; Kurtzberg, J ; Berens, ME ; Halperin, EC</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h352t-78d10fc03fff7069c09cf7fab902de8f1648af9b6e2a94304fd51c3148bfe84f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cerebellar Neoplasms - genetics</topic><topic>Cerebellar Neoplasms - pathology</topic><topic>Child, Preschool</topic><topic>Female</topic><topic>Gene Amplification</topic><topic>Genotype</topic><topic>Humans</topic><topic>Immunosorbent Techniques</topic><topic>Karyotyping</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Medulloblastoma - genetics</topic><topic>Medulloblastoma - pathology</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Microscopy, Electron</topic><topic>Neoplasm Transplantation</topic><topic>Neurology</topic><topic>Oncogenes</topic><topic>Phenotype</topic><topic>Radioimmunoassay</topic><topic>Transplantation, Heterologous</topic><topic>Tumors of the nervous system. Phacomatoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Friedman, HS</creatorcontrib><creatorcontrib>Burger, PC</creatorcontrib><creatorcontrib>Bigner, SH</creatorcontrib><creatorcontrib>Trojanowski, JQ</creatorcontrib><creatorcontrib>Brodeur, GM</creatorcontrib><creatorcontrib>He, XM</creatorcontrib><creatorcontrib>Wikstrand, CJ</creatorcontrib><creatorcontrib>Kurtzberg, J</creatorcontrib><creatorcontrib>Berens, ME</creatorcontrib><creatorcontrib>Halperin, EC</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The American journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Friedman, HS</au><au>Burger, PC</au><au>Bigner, SH</au><au>Trojanowski, JQ</au><au>Brodeur, GM</au><au>He, XM</au><au>Wikstrand, CJ</au><au>Kurtzberg, J</au><au>Berens, ME</au><au>Halperin, EC</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenotypic and genotypic analysis of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc</atitle><jtitle>The American journal of pathology</jtitle><addtitle>Am J Pathol</addtitle><date>1988-03-01</date><risdate>1988</risdate><volume>130</volume><issue>3</issue><spage>472</spage><epage>484</epage><pages>472-484</pages><issn>0002-9440</issn><eissn>1525-2191</eissn><coden>AJPAA4</coden><abstract>D341 Med is a new continuous cell line and transplantable xenograft derived from a cerebellar medulloblastoma. This line grew in vitro in suspension culture with spontaneous macroscopic spheroid formation and demonstrated 20-fold amplification of c-myc. Cultured D341 Med cells injected subcutaneously into athymic mice grew as markedly cellular, highly invasive undifferentiated neoplasms. Intracranial tumors grew as markedly cellular mitotically active neoplasms largely located within the subarachnoid space or lining the ventricular system. Immunocytochemical analysis of the cell line and SQ tumors revealed the high (NFP-H) and middle (NFP-M) molecular weight (Mr) neurofilament proteins (NFPs). Immunoblots demonstrated the presence of molecular species that co-migrated with authentic human NFP-H and NFP-M. This cell line and transplantable xenograft may allow, in conjunction with the authors' other models of human medulloblastoma, analysis of the heterogeneous biologic properties and therapeutic sensitivity of this tumor.</abstract><cop>Bethesda, MD</cop><pub>ASIP</pub><pmid>3279793</pmid><tpages>13</tpages></addata></record> |
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| ispartof | The American journal of pathology, 1988-03, Vol.130 (3), p.472-484 |
| issn | 0002-9440 1525-2191 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Biological and medical sciences Cell Line Cerebellar Neoplasms - genetics Cerebellar Neoplasms - pathology Child, Preschool Female Gene Amplification Genotype Humans Immunosorbent Techniques Karyotyping Male Medical sciences Medulloblastoma - genetics Medulloblastoma - pathology Mice Mice, Nude Microscopy, Electron Neoplasm Transplantation Neurology Oncogenes Phenotype Radioimmunoassay Transplantation, Heterologous Tumors of the nervous system. Phacomatoses |
| title | Phenotypic and genotypic analysis of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc |
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