Regulation of fibronectin synthesis by interleukin-1 and interleukin-6 in rat hepatocytes
The authors have observed previously that recombinant human interleukin-1 (rhIL-1) administered into rats increased plasma fibronectin (Fn) level concomitant with the increase of Fn in the liver. Because IL-1 induces interleukin-6 (IL-6) in certain cell types, the IL-1 effect might be mediated by IL...
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| Veröffentlicht in: | The American journal of pathology 1990-01, Vol.136 (1), p.39-47 |
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| creator | Hagiwara, T Suzuki, H Kono, I Kashiwagi, H Akiyama, Y Onozaki, K |
| description | The authors have observed previously that recombinant human interleukin-1 (rhIL-1) administered into rats increased plasma fibronectin (Fn) level concomitant with the increase of Fn in the liver. Because IL-1 induces interleukin-6 (IL-6) in certain cell types, the IL-1 effect might be mediated by IL-6. To evaluate this possibility, the effect of recombinant human interleukin-6 (rhIL-6), rhIL-1 alpha, and rhIL-1 beta on Fn synthesis in cultured rat hepatocytes was studied. It was shown that rhIL-6 increased Fn synthesis in hepatocytes, in contrast, rhIL-1 alpha, rhIL-I beta and TNF did not have any effect on Fn synthesis. When we studied the interaction of IL-1 and IL-6, IL-1 did not exhibit any synergistic effect with IL-6. Conditioned medium (CM) from rhIL-1 stimulated peripheral blood monocytes (PBM) increased the Fn synthesis, and its activity was neutralized significantly by anti-rhIL-6 antibodies. The CM from rhIL-1-stimulated PBM was analyzed by enzyme linked immunosorbent assay and revealed the increase of IL-6. Furthermore, it was found that intraperitoneal administration of rhIL-1 induced IL-6 into blood. The administration of rhIL-6 into rats increased circulating Fn levels. These results strongly suggest that the in vivo effect of IL-1 on Fn synthesis is mediated by IL-6. |
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Because IL-1 induces interleukin-6 (IL-6) in certain cell types, the IL-1 effect might be mediated by IL-6. To evaluate this possibility, the effect of recombinant human interleukin-6 (rhIL-6), rhIL-1 alpha, and rhIL-1 beta on Fn synthesis in cultured rat hepatocytes was studied. It was shown that rhIL-6 increased Fn synthesis in hepatocytes, in contrast, rhIL-1 alpha, rhIL-I beta and TNF did not have any effect on Fn synthesis. When we studied the interaction of IL-1 and IL-6, IL-1 did not exhibit any synergistic effect with IL-6. Conditioned medium (CM) from rhIL-1 stimulated peripheral blood monocytes (PBM) increased the Fn synthesis, and its activity was neutralized significantly by anti-rhIL-6 antibodies. The CM from rhIL-1-stimulated PBM was analyzed by enzyme linked immunosorbent assay and revealed the increase of IL-6. Furthermore, it was found that intraperitoneal administration of rhIL-1 induced IL-6 into blood. The administration of rhIL-6 into rats increased circulating Fn levels. These results strongly suggest that the in vivo effect of IL-1 on Fn synthesis is mediated by IL-6.</description><identifier>ISSN: 0002-9440</identifier><identifier>EISSN: 1525-2191</identifier><identifier>PMID: 2404418</identifier><identifier>CODEN: AJPAA4</identifier><language>eng</language><publisher>Bethesda, MD: ASIP</publisher><subject>Animals ; Biological and medical sciences ; Biological Factors - pharmacology ; Cell physiology ; Cells, Cultured ; Child ; Culture Media - pharmacology ; Cytokines ; Fibronectins - blood ; Fibronectins - genetics ; Fibronectins - metabolism ; Fundamental and applied biological sciences. Psychology ; Humans ; Interleukin-1 - metabolism ; Interleukin-1 - pharmacology ; Interleukin-6 - metabolism ; Interleukin-6 - pharmacology ; Liver - cytology ; Liver - drug effects ; Liver - metabolism ; Male ; Molecular and cellular biology ; Rats ; Rats, Inbred Strains ; Recombinant Proteins - pharmacology ; RNA, Messenger - drug effects ; RNA, Messenger - metabolism</subject><ispartof>The American journal of pathology, 1990-01, Vol.136 (1), p.39-47</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1877464/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1877464/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,4012,53774,53776</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4427323$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2404418$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hagiwara, T</creatorcontrib><creatorcontrib>Suzuki, H</creatorcontrib><creatorcontrib>Kono, I</creatorcontrib><creatorcontrib>Kashiwagi, H</creatorcontrib><creatorcontrib>Akiyama, Y</creatorcontrib><creatorcontrib>Onozaki, K</creatorcontrib><title>Regulation of fibronectin synthesis by interleukin-1 and interleukin-6 in rat hepatocytes</title><title>The American journal of pathology</title><addtitle>Am J Pathol</addtitle><description>The authors have observed previously that recombinant human interleukin-1 (rhIL-1) administered into rats increased plasma fibronectin (Fn) level concomitant with the increase of Fn in the liver. Because IL-1 induces interleukin-6 (IL-6) in certain cell types, the IL-1 effect might be mediated by IL-6. To evaluate this possibility, the effect of recombinant human interleukin-6 (rhIL-6), rhIL-1 alpha, and rhIL-1 beta on Fn synthesis in cultured rat hepatocytes was studied. It was shown that rhIL-6 increased Fn synthesis in hepatocytes, in contrast, rhIL-1 alpha, rhIL-I beta and TNF did not have any effect on Fn synthesis. When we studied the interaction of IL-1 and IL-6, IL-1 did not exhibit any synergistic effect with IL-6. Conditioned medium (CM) from rhIL-1 stimulated peripheral blood monocytes (PBM) increased the Fn synthesis, and its activity was neutralized significantly by anti-rhIL-6 antibodies. The CM from rhIL-1-stimulated PBM was analyzed by enzyme linked immunosorbent assay and revealed the increase of IL-6. Furthermore, it was found that intraperitoneal administration of rhIL-1 induced IL-6 into blood. The administration of rhIL-6 into rats increased circulating Fn levels. These results strongly suggest that the in vivo effect of IL-1 on Fn synthesis is mediated by IL-6.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Factors - pharmacology</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Child</subject><subject>Culture Media - pharmacology</subject><subject>Cytokines</subject><subject>Fibronectins - blood</subject><subject>Fibronectins - genetics</subject><subject>Fibronectins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Interleukin-1 - metabolism</subject><subject>Interleukin-1 - pharmacology</subject><subject>Interleukin-6 - metabolism</subject><subject>Interleukin-6 - pharmacology</subject><subject>Liver - cytology</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Molecular and cellular biology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Recombinant Proteins - pharmacology</subject><subject>RNA, Messenger - drug effects</subject><subject>RNA, Messenger - metabolism</subject><issn>0002-9440</issn><issn>1525-2191</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkFtLxDAQhYsoul5-gtAH9a3QXJqmL4KIN1gQRB98CtN0us2aTdckVfbfG3BZ9Gk4cw7fGWYvm5GKVgUlDdnPZmVZ0qLhvDzKjkNYJimYLA-zQ8pLzomcZe8vuJgsRDO6fOzz3rR-dKijcXnYuDhgMCFvN7lxEb3F6cO4guTgun8bkVTuIeYDriGOehMxnGYHPdiAZ9t5kr3d373ePhbz54en25t5MTBaxkIT3TUg-q6iDTIkLYDs0sVEy4r3umaEC0aBa1nXBFsBqHVdSSiRCik5sJPs-pe7ntoVdhpd9GDV2psV-I0awaj_jjODWoxfiiQiFzwBrrYAP35OGKJamaDRWnA4TkHVTdVUQlYpeP63aVexfWbyL7Y-BA229-C0CbsY57RmlKXY5W9sMIvh23hUYQXWJihRsFwTJhRRrGE_s4eNfQ</recordid><startdate>19900101</startdate><enddate>19900101</enddate><creator>Hagiwara, T</creator><creator>Suzuki, H</creator><creator>Kono, I</creator><creator>Kashiwagi, H</creator><creator>Akiyama, Y</creator><creator>Onozaki, K</creator><general>ASIP</general><general>American Society for Investigative Pathology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19900101</creationdate><title>Regulation of fibronectin synthesis by interleukin-1 and interleukin-6 in rat hepatocytes</title><author>Hagiwara, T ; Suzuki, H ; Kono, I ; Kashiwagi, H ; Akiyama, Y ; Onozaki, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h320t-c1cd9a6fd529e3e1baa8d4401c854fc7314632a4c8771eb6aecc758a0e26884a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Factors - pharmacology</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Child</topic><topic>Culture Media - pharmacology</topic><topic>Cytokines</topic><topic>Fibronectins - blood</topic><topic>Fibronectins - genetics</topic><topic>Fibronectins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Interleukin-1 - metabolism</topic><topic>Interleukin-1 - pharmacology</topic><topic>Interleukin-6 - metabolism</topic><topic>Interleukin-6 - pharmacology</topic><topic>Liver - cytology</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Molecular and cellular biology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Recombinant Proteins - pharmacology</topic><topic>RNA, Messenger - drug effects</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hagiwara, T</creatorcontrib><creatorcontrib>Suzuki, H</creatorcontrib><creatorcontrib>Kono, I</creatorcontrib><creatorcontrib>Kashiwagi, H</creatorcontrib><creatorcontrib>Akiyama, Y</creatorcontrib><creatorcontrib>Onozaki, K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The American journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hagiwara, T</au><au>Suzuki, H</au><au>Kono, I</au><au>Kashiwagi, H</au><au>Akiyama, Y</au><au>Onozaki, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of fibronectin synthesis by interleukin-1 and interleukin-6 in rat hepatocytes</atitle><jtitle>The American journal of pathology</jtitle><addtitle>Am J Pathol</addtitle><date>1990-01-01</date><risdate>1990</risdate><volume>136</volume><issue>1</issue><spage>39</spage><epage>47</epage><pages>39-47</pages><issn>0002-9440</issn><eissn>1525-2191</eissn><coden>AJPAA4</coden><abstract>The authors have observed previously that recombinant human interleukin-1 (rhIL-1) administered into rats increased plasma fibronectin (Fn) level concomitant with the increase of Fn in the liver. Because IL-1 induces interleukin-6 (IL-6) in certain cell types, the IL-1 effect might be mediated by IL-6. To evaluate this possibility, the effect of recombinant human interleukin-6 (rhIL-6), rhIL-1 alpha, and rhIL-1 beta on Fn synthesis in cultured rat hepatocytes was studied. It was shown that rhIL-6 increased Fn synthesis in hepatocytes, in contrast, rhIL-1 alpha, rhIL-I beta and TNF did not have any effect on Fn synthesis. When we studied the interaction of IL-1 and IL-6, IL-1 did not exhibit any synergistic effect with IL-6. Conditioned medium (CM) from rhIL-1 stimulated peripheral blood monocytes (PBM) increased the Fn synthesis, and its activity was neutralized significantly by anti-rhIL-6 antibodies. The CM from rhIL-1-stimulated PBM was analyzed by enzyme linked immunosorbent assay and revealed the increase of IL-6. Furthermore, it was found that intraperitoneal administration of rhIL-1 induced IL-6 into blood. The administration of rhIL-6 into rats increased circulating Fn levels. These results strongly suggest that the in vivo effect of IL-1 on Fn synthesis is mediated by IL-6.</abstract><cop>Bethesda, MD</cop><pub>ASIP</pub><pmid>2404418</pmid><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Biological and medical sciences Biological Factors - pharmacology Cell physiology Cells, Cultured Child Culture Media - pharmacology Cytokines Fibronectins - blood Fibronectins - genetics Fibronectins - metabolism Fundamental and applied biological sciences. Psychology Humans Interleukin-1 - metabolism Interleukin-1 - pharmacology Interleukin-6 - metabolism Interleukin-6 - pharmacology Liver - cytology Liver - drug effects Liver - metabolism Male Molecular and cellular biology Rats Rats, Inbred Strains Recombinant Proteins - pharmacology RNA, Messenger - drug effects RNA, Messenger - metabolism |
| title | Regulation of fibronectin synthesis by interleukin-1 and interleukin-6 in rat hepatocytes |
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