Activator recruitment by the general transcription machinery: X-ray structural analysis of the Oct-1 POU domain/human U1 octamer/SNAP190 peptide ternary complex
Transcriptional activation of the human U1 snRNA genes is dependent on a noncanonical octamer element contained within an upstream enhancer. The U1 octamer only weakly recruits the Oct-1 POU domain, although recruitment is stimulated by a peptide containing the Oct-1-binding domain of SNAP190. Struc...
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| Veröffentlicht in: | Genes & development 2002-11, Vol.16 (21), p.2772-2777 |
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| container_title | Genes & development |
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| creator | Hovde, Stacy Hinkley, Craig S Strong, Katie Brooks, Aimee Gu, Liping Henry, R William Geiger, James |
| description | Transcriptional activation of the human U1 snRNA genes is dependent on a noncanonical octamer element contained within an upstream enhancer. The U1 octamer only weakly recruits the Oct-1 POU domain, although recruitment is stimulated by a peptide containing the Oct-1-binding domain of SNAP190. Structural analysis of the Oct-1 POU domain/U1 octamer/SNAP190 peptide complex revealed that SNAP190 makes extensive protein contacts with the Oct-1 POU-specific domain and with the DNA phosphate backbone within the enhancer. Although SNAP190 and OCA-B both interact with the Oct-1 POU domain through the same Oct-1 interface, a single nucleotide within the U1 octamer ablates OCA-B recruitment without compromising activator recruitment by SNAP190. |
| doi_str_mv | 10.1101/gad.1021002 |
| format | Article |
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Although SNAP190 and OCA-B both interact with the Oct-1 POU domain through the same Oct-1 interface, a single nucleotide within the U1 octamer ablates OCA-B recruitment without compromising activator recruitment by SNAP190.</description><subject>DNA-Binding Proteins - chemistry</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Host Cell Factor C1</subject><subject>Humans</subject><subject>Nucleic Acid Conformation</subject><subject>Octamer Transcription Factor-1</subject><subject>Protein Binding</subject><subject>Protein Conformation</subject><subject>Protein Structure, Tertiary - genetics</subject><subject>Research Communication</subject><subject>RNA, Small Nuclear - chemistry</subject><subject>RNA, Small Nuclear - genetics</subject><subject>Transcription Factors - chemistry</subject><subject>Transcription Factors - genetics</subject><subject>Transcriptional Activation</subject><issn>0890-9369</issn><issn>1549-5477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS1ERYfCij3yik2VznXixDESi1HFn1R1KpWR2FmO7cwYJXawnYq8DY-KS0f8rLry4n736Ph-CL0icEEIkPVe6gsCJQEon6AVqSkvasrYU7SClkPBq4afoucxfgOABprmGTolJSWUVbBCPzcq2TuZfMDBqDDbNBqXcLfgdDB4b5wJcsApSBdVsFOy3uFRqoPNg-Ut_loEueCYwqzSfE9KJ4cl2oh9_zthq1JB8M12h7UfpXXrwzxKh3cEe5XkaML69npzQzjgyeR0bXAywcmwYOXHaTA_XqCTXg7RvDy-Z2j34f2Xy0_F1fbj58vNVaEosFSwpoISTNkw3qmK9rxvgXOtQHPaKdJL1ne6BUI4ZVAbTnotjdS9KrmudNNWZ-jdQ-40d6PRKl8h_0dMwY65jfDSiv8nzh7E3t8J0jLKaN5_c9wP_vtsYhKjjcoMg3TGz1Gwsqlo9vEoSNq6BlryDJ4_gCr4GIPp_5QhIO7Ni2xeHM1n-vW__f-yR9XVL9HorS4</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>Hovde, Stacy</creator><creator>Hinkley, Craig S</creator><creator>Strong, Katie</creator><creator>Brooks, Aimee</creator><creator>Gu, Liping</creator><creator>Henry, R William</creator><creator>Geiger, James</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20021101</creationdate><title>Activator recruitment by the general transcription machinery: X-ray structural analysis of the Oct-1 POU domain/human U1 octamer/SNAP190 peptide ternary complex</title><author>Hovde, Stacy ; 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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics Host Cell Factor C1 Humans Nucleic Acid Conformation Octamer Transcription Factor-1 Protein Binding Protein Conformation Protein Structure, Tertiary - genetics Research Communication RNA, Small Nuclear - chemistry RNA, Small Nuclear - genetics Transcription Factors - chemistry Transcription Factors - genetics Transcriptional Activation |
| title | Activator recruitment by the general transcription machinery: X-ray structural analysis of the Oct-1 POU domain/human U1 octamer/SNAP190 peptide ternary complex |
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