USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products

We present a method that allows simultaneous fusion and cloning of multiple PCR products in a rapid and efficient manner. The procedure is based on the use of PCR primers that contain a single deoxyuridine residue near their 5' end. Treatment of the PCR products with a commercial deoxyuridine-e...

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Veröffentlicht in:	Nucleic acids research 2007-04, Vol.35 (7), p.e55-e55
Hauptverfasser:	Geu-Flores, Fernando, Nour-Eldin, Hussam H, Nielsen, Morten T, Halkier, Barbara A
Format:	Artikel
Sprache:	eng
Schlagworte:	Artificial Gene Fusion - methods Chromatography, Agarose Cloning, Molecular - methods Methods Online Open Reading Frames Polymerase Chain Reaction
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