Molecular Classification of Crohn's Disease and Ulcerative Colitis Patients Using Transcriptional Profiles in Peripheral Blood Mononuclear Cells
Ulcerative colitis (UC) and Crohn's disease (CD) are common inflammatory bowel diseases producing intestinal inflammation and tissue damage. Although emerging evidence suggests these diseases are distinct, ∼10% of patients remain classified as indeterminate inflammatory bowel disease even after...
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| Veröffentlicht in: | The Journal of molecular diagnostics : JMD 2006-02, Vol.8 (1), p.51-61 |
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| creator | Burczynski, Michael E. Peterson, Ron L. Twine, Natalie C. Zuberek, Krystyna A. Brodeur, Brendan J. Casciotti, Lori Maganti, Vasu Reddy, Padma S. Strahs, Andrew Immermann, Fred Spinelli, Walter Schwertschlag, Ulrich Slager, Anna M. Cotreau, Monette M. Dorner, Andrew J. |
| description | Ulcerative colitis (UC) and Crohn's disease (CD) are common inflammatory bowel diseases producing intestinal inflammation and tissue damage. Although emerging evidence suggests these diseases are distinct, ∼10% of patients remain classified as indeterminate inflammatory bowel disease even after invasive colonoscopy intended for diagnosis. A molecular diagnostic assay using a clinically accessible tissue would greatly assist in the classification of these diseases. In the present study we assessed transcriptional profiles in peripheral blood mononuclear cells from 42 healthy individuals, 59 CD patients, and 26 UC patients by hybridization to microarrays interrogating more than 22,000 sequences. Supervised analysis identified a set of 12 genes that distinguished UC and CD patient samples with high accuracy. The alterations in transcript levels observed by microarray were verified by real-time polymerase chain reaction. The results suggest that a peripheral blood mononuclear cell-based gene expression signature can provide a molecular biomarker that can complement the standard dia-gnosis of UC and CD. |
| doi_str_mv | 10.2353/jmoldx.2006.050079 |
| format | Article |
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Although emerging evidence suggests these diseases are distinct, ∼10% of patients remain classified as indeterminate inflammatory bowel disease even after invasive colonoscopy intended for diagnosis. A molecular diagnostic assay using a clinically accessible tissue would greatly assist in the classification of these diseases. In the present study we assessed transcriptional profiles in peripheral blood mononuclear cells from 42 healthy individuals, 59 CD patients, and 26 UC patients by hybridization to microarrays interrogating more than 22,000 sequences. Supervised analysis identified a set of 12 genes that distinguished UC and CD patient samples with high accuracy. The alterations in transcript levels observed by microarray were verified by real-time polymerase chain reaction. The results suggest that a peripheral blood mononuclear cell-based gene expression signature can provide a molecular biomarker that can complement the standard dia-gnosis of UC and CD.</description><identifier>ISSN: 1525-1578</identifier><identifier>EISSN: 1943-7811</identifier><identifier>DOI: 10.2353/jmoldx.2006.050079</identifier><identifier>PMID: 16436634</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Case-Control Studies ; Colitis, Ulcerative - blood ; Colitis, Ulcerative - diagnosis ; Crohn Disease - blood ; Crohn Disease - diagnosis ; Gene Expression Profiling ; Humans ; Leukocytes, Mononuclear - chemistry ; Male ; Molecular Diagnostic Techniques ; Regular ; Reverse Transcriptase Polymerase Chain Reaction - methods</subject><ispartof>The Journal of molecular diagnostics : JMD, 2006-02, Vol.8 (1), p.51-61</ispartof><rights>2006 American Society for Investigative Pathology and Association for Molecular Pathology</rights><rights>Copyright © American Society for Investigative Pathology and the Association for Molecular Pathology 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c547t-e12537c06b6defcfb938a790c2e880e873ff533b19272c84a8400e41c47839d93</citedby><cites>FETCH-LOGICAL-c547t-e12537c06b6defcfb938a790c2e880e873ff533b19272c84a8400e41c47839d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1525157810602929$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16436634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Burczynski, Michael E.</creatorcontrib><creatorcontrib>Peterson, Ron L.</creatorcontrib><creatorcontrib>Twine, Natalie C.</creatorcontrib><creatorcontrib>Zuberek, Krystyna A.</creatorcontrib><creatorcontrib>Brodeur, Brendan J.</creatorcontrib><creatorcontrib>Casciotti, Lori</creatorcontrib><creatorcontrib>Maganti, Vasu</creatorcontrib><creatorcontrib>Reddy, Padma S.</creatorcontrib><creatorcontrib>Strahs, Andrew</creatorcontrib><creatorcontrib>Immermann, Fred</creatorcontrib><creatorcontrib>Spinelli, Walter</creatorcontrib><creatorcontrib>Schwertschlag, Ulrich</creatorcontrib><creatorcontrib>Slager, Anna M.</creatorcontrib><creatorcontrib>Cotreau, Monette M.</creatorcontrib><creatorcontrib>Dorner, Andrew J.</creatorcontrib><title>Molecular Classification of Crohn's Disease and Ulcerative Colitis Patients Using Transcriptional Profiles in Peripheral Blood Mononuclear Cells</title><title>The Journal of molecular diagnostics : JMD</title><addtitle>J Mol Diagn</addtitle><description>Ulcerative colitis (UC) and Crohn's disease (CD) are common inflammatory bowel diseases producing intestinal inflammation and tissue damage. Although emerging evidence suggests these diseases are distinct, ∼10% of patients remain classified as indeterminate inflammatory bowel disease even after invasive colonoscopy intended for diagnosis. A molecular diagnostic assay using a clinically accessible tissue would greatly assist in the classification of these diseases. In the present study we assessed transcriptional profiles in peripheral blood mononuclear cells from 42 healthy individuals, 59 CD patients, and 26 UC patients by hybridization to microarrays interrogating more than 22,000 sequences. Supervised analysis identified a set of 12 genes that distinguished UC and CD patient samples with high accuracy. The alterations in transcript levels observed by microarray were verified by real-time polymerase chain reaction. The results suggest that a peripheral blood mononuclear cell-based gene expression signature can provide a molecular biomarker that can complement the standard dia-gnosis of UC and CD.</description><subject>Adult</subject><subject>Case-Control Studies</subject><subject>Colitis, Ulcerative - blood</subject><subject>Colitis, Ulcerative - diagnosis</subject><subject>Crohn Disease - blood</subject><subject>Crohn Disease - diagnosis</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - chemistry</subject><subject>Male</subject><subject>Molecular Diagnostic Techniques</subject><subject>Regular</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><issn>1525-1578</issn><issn>1943-7811</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuO0zAUhiMEYoaBF2CBLCHBqsWOndiREBKUqzQjupiuLdc5aTxy7Y5P0hnegkfGUSouG1a-fec___FfFM8ZXZa84m9u9tG398uS0npJK0pl86A4Z43gC6kYe5j3VVktWCXVWfEE8YZSJkRdPi7OWC14XXNxXvy8ih7s6E0iK28QXeesGVwMJHZklWIfXiP56BAMAjGhJRtvIWXiCGQVvRscknU-QhiQbNCFHblOJqBN7jDJGE_WKXbOAxIXyBryfZ8FPPngY2zJVQwxjNbDZAC8x6fFo854hGen9aLYfP50vfq6uPz-5dvq_eXCVkIOC2BlxaWl9bZuobPdtuHKyIbaEpSioCTvuorzLWtKWVoljBKUgmBWSMWbtuEXxbtZ9zBu99DaPEB2pQ_J7U36oaNx-t-X4Hq9i0fNVC0rybPAq5NAircj4KD3Dm0ewQSII2pJpWCsZBksZ9CmiJig-92EUT0Fqecg9RSknoPMRS_-tven5JRcBl7OQO92_Z1LoHFvvM84y3qt0kxXU--3MwX5K48Okkabs7LQ5go76Da6_7n4BQwiv8Q</recordid><startdate>20060201</startdate><enddate>20060201</enddate><creator>Burczynski, Michael E.</creator><creator>Peterson, Ron L.</creator><creator>Twine, Natalie C.</creator><creator>Zuberek, Krystyna A.</creator><creator>Brodeur, Brendan J.</creator><creator>Casciotti, Lori</creator><creator>Maganti, Vasu</creator><creator>Reddy, Padma S.</creator><creator>Strahs, Andrew</creator><creator>Immermann, Fred</creator><creator>Spinelli, Walter</creator><creator>Schwertschlag, Ulrich</creator><creator>Slager, Anna M.</creator><creator>Cotreau, Monette M.</creator><creator>Dorner, Andrew J.</creator><general>Elsevier Inc</general><general>ASIP</general><general>American Society for Investigative Pathology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20060201</creationdate><title>Molecular Classification of Crohn's Disease and Ulcerative Colitis Patients Using Transcriptional Profiles in Peripheral Blood Mononuclear Cells</title><author>Burczynski, Michael E. ; 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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adult Case-Control Studies Colitis, Ulcerative - blood Colitis, Ulcerative - diagnosis Crohn Disease - blood Crohn Disease - diagnosis Gene Expression Profiling Humans Leukocytes, Mononuclear - chemistry Male Molecular Diagnostic Techniques Regular Reverse Transcriptase Polymerase Chain Reaction - methods |
| title | Molecular Classification of Crohn's Disease and Ulcerative Colitis Patients Using Transcriptional Profiles in Peripheral Blood Mononuclear Cells |
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