Low Copy Number DNA Template Can Render Polymerase Chain Reaction Error Prone in a Sequence-Dependent Manner

Paraffin-embedded tissue is an important source of material for molecular pathology and genetic investigations. We used DNA isolated from microdissected formalin-fixed, paraffin-embedded gastric tumors for mutation analysis of a region of the human gene for uracil-DNA glycosylase ( UNG), encoding th...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	The Journal of molecular diagnostics : JMD 2005-02, Vol.7 (1), p.36-39
Hauptverfasser:	Akbari, Mansour, Doré Hansen, Marianne, Halgunset, Jostein, Skorpen, Frank, Krokan, Hans E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Catalytic Domain - genetics DNA Glycosylases - genetics DNA Mutational Analysis DNA, Neoplasm - analysis Humans Mutagenesis Mutation - genetics Polymerase Chain Reaction Regular Templates, Genetic Uracil-DNA Glycosidase
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!