Neuropilin-1 Participates in Wound Angiogenesis
Angiogenesis, the formation of new capillaries from existing vasculature, plays an essential role in tissue repair. The rapid onset and predominance of proangiogenic factors optimizes healing in damaged tissues. One factor that directly mediates wound vessel angiogenesis is vascular endothelial grow...
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| Veröffentlicht in: | The American journal of pathology 2002, Vol.160 (1), p.289-296 |
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| creator | Matthies, Annette M. Low, Quentin E.H. Lingen, Mark W. DiPietro, Luisa A. |
| description | Angiogenesis, the formation of new capillaries from existing vasculature, plays an essential role in tissue repair. The rapid onset and predominance of proangiogenic factors optimizes healing in damaged tissues. One factor that directly mediates wound vessel angiogenesis is vascular endothelial growth factor (VEGF). Although much is known about the biology of VEGF and its cognate receptors, VEGFR1 and VEGFR2, the role of a recently identified co-receptor for VEGF, neuropilin-1, is not well understood. Using a murine model of dermal wound repair, we found that neuropilin-1 was abundantly expressed on new vasculature in healing wounds. Moreover, mice treated with anti-neuropilin-1 antibodies exhibited a significant decrease in vascular density within these wounds (67% decrease,
P = 0.0132). In
in vitro assays, VEGF induced formation of endothelial cord-like structures on collagen gel and endothelial cell migration toward VEGF was inhibited by antibodies directed against neuropilin-1. These results provide both
in vitro and
in vivo evidence for a critical role of neuropilin-1 in wound angiogenesis. |
| doi_str_mv | 10.1016/S0002-9440(10)64372-6 |
| format | Article |
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P = 0.0132). In
in vitro assays, VEGF induced formation of endothelial cord-like structures on collagen gel and endothelial cell migration toward VEGF was inhibited by antibodies directed against neuropilin-1. These results provide both
in vitro and
in vivo evidence for a critical role of neuropilin-1 in wound angiogenesis.</description><identifier>ISSN: 0002-9440</identifier><identifier>EISSN: 1525-2191</identifier><identifier>DOI: 10.1016/S0002-9440(10)64372-6</identifier><identifier>PMID: 11786422</identifier><identifier>CODEN: AJPAA4</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Cells, Cultured ; Degeneration. Regeneration. Wound healing. Graft ; Endothelial Growth Factors - genetics ; Female ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; Lymphokines - genetics ; Mice ; Mice, Inbred BALB C ; Neovascularization, Physiologic - physiology ; Nerve Tissue Proteins - physiology ; Neuropilin-1 ; Protein Isoforms - genetics ; Receptor Protein-Tyrosine Kinases - genetics ; Receptors, Growth Factor - genetics ; Receptors, Vascular Endothelial Growth Factor ; Regular ; RNA, Messenger - metabolism ; Skin - injuries ; Tissue Distribution ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Wound Healing - physiology ; Wounds and Injuries - metabolism ; Wounds and Injuries - physiopathology</subject><ispartof>The American journal of pathology, 2002, Vol.160 (1), p.289-296</ispartof><rights>2002 American Society for Investigative Pathology</rights><rights>2002 INIST-CNRS</rights><rights>Copyright American Society for Investigative Pathology Jan 2002</rights><rights>Copyright © 2002, American Society for Investigative Pathology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c617t-defb35df2dab79429fcbb804feda2da91ad5c81c19e52b375bf23954b265ce9a3</citedby><cites>FETCH-LOGICAL-c617t-defb35df2dab79429fcbb804feda2da91ad5c81c19e52b375bf23954b265ce9a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1867138/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0002-9440(10)64372-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,3552,4026,27930,27931,27932,46002,53798,53800</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13443551$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11786422$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matthies, Annette M.</creatorcontrib><creatorcontrib>Low, Quentin E.H.</creatorcontrib><creatorcontrib>Lingen, Mark W.</creatorcontrib><creatorcontrib>DiPietro, Luisa A.</creatorcontrib><title>Neuropilin-1 Participates in Wound Angiogenesis</title><title>The American journal of pathology</title><addtitle>Am J Pathol</addtitle><description>Angiogenesis, the formation of new capillaries from existing vasculature, plays an essential role in tissue repair. The rapid onset and predominance of proangiogenic factors optimizes healing in damaged tissues. One factor that directly mediates wound vessel angiogenesis is vascular endothelial growth factor (VEGF). Although much is known about the biology of VEGF and its cognate receptors, VEGFR1 and VEGFR2, the role of a recently identified co-receptor for VEGF, neuropilin-1, is not well understood. Using a murine model of dermal wound repair, we found that neuropilin-1 was abundantly expressed on new vasculature in healing wounds. Moreover, mice treated with anti-neuropilin-1 antibodies exhibited a significant decrease in vascular density within these wounds (67% decrease,
P = 0.0132). In
in vitro assays, VEGF induced formation of endothelial cord-like structures on collagen gel and endothelial cell migration toward VEGF was inhibited by antibodies directed against neuropilin-1. These results provide both
in vitro and
in vivo evidence for a critical role of neuropilin-1 in wound angiogenesis.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Degeneration. Regeneration. Wound healing. Graft</subject><subject>Endothelial Growth Factors - genetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>Lymphokines - genetics</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Neovascularization, Physiologic - physiology</subject><subject>Nerve Tissue Proteins - physiology</subject><subject>Neuropilin-1</subject><subject>Protein Isoforms - genetics</subject><subject>Receptor Protein-Tyrosine Kinases - genetics</subject><subject>Receptors, Growth Factor - genetics</subject><subject>Receptors, Vascular Endothelial Growth Factor</subject><subject>Regular</subject><subject>RNA, Messenger - metabolism</subject><subject>Skin - injuries</subject><subject>Tissue Distribution</subject><subject>Vascular Endothelial Growth Factor A</subject><subject>Vascular Endothelial Growth Factors</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Wound Healing - physiology</subject><subject>Wounds and Injuries - metabolism</subject><subject>Wounds and Injuries - physiopathology</subject><issn>0002-9440</issn><issn>1525-2191</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU-LFDEQxYMo7rj6EZRBUPTQbirppDsXZVn8B4sKKh5DOqmeydCT9CbdK357MzvDjnrxFFL51at6eYQ8BvoKKMizr5RSVqm6pi-AvpQ1b1gl75AFCCYqBgruksUtckIe5LwpV8lbep-cADStrBlbkLNPOKc4-sGHCpZfTJq89aOZMC99WP6Ic3DL87DycYUBs88Pyb3eDBkfHc5T8v3d228XH6rLz-8_XpxfVlZCM1UO-44L1zNnukbVTPW261pa9-hMqSkwTtgWLCgUrOON6HrGlag7JoVFZfgpeb3XHedui85imJIZ9Jj81qRfOhqv_34Jfq1X8VpDKxvgbRF4fhBI8WrGPOmtzxaHwQSMc9YFkpIxKODTf8BNnFMo5jSDVkmQLS2Q2EM2xZwT9rebANW7PPRNHnr32bvSTR5alr4nf9o4dh0CKMCzA2CyNUOfTLA-Hzle11wIONpZ-9X6p0-o89YMQ5EFbTYjyDJWs1YV8M0exJLOtceks_UYLLrSZCftov_Pzr8Boum0jA</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>Matthies, Annette M.</creator><creator>Low, Quentin E.H.</creator><creator>Lingen, Mark W.</creator><creator>DiPietro, Luisa A.</creator><general>Elsevier Inc</general><general>ASIP</general><general>American Society for Investigative Pathology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2002</creationdate><title>Neuropilin-1 Participates in Wound Angiogenesis</title><author>Matthies, Annette M. ; Low, Quentin E.H. ; Lingen, Mark W. ; DiPietro, Luisa A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c617t-defb35df2dab79429fcbb804feda2da91ad5c81c19e52b375bf23954b265ce9a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Degeneration. Regeneration. Wound healing. Graft</topic><topic>Endothelial Growth Factors - genetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>Lymphokines - genetics</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Neovascularization, Physiologic - physiology</topic><topic>Nerve Tissue Proteins - physiology</topic><topic>Neuropilin-1</topic><topic>Protein Isoforms - genetics</topic><topic>Receptor Protein-Tyrosine Kinases - genetics</topic><topic>Receptors, Growth Factor - genetics</topic><topic>Receptors, Vascular Endothelial Growth Factor</topic><topic>Regular</topic><topic>RNA, Messenger - metabolism</topic><topic>Skin - injuries</topic><topic>Tissue Distribution</topic><topic>Vascular Endothelial Growth Factor A</topic><topic>Vascular Endothelial Growth Factors</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Wound Healing - physiology</topic><topic>Wounds and Injuries - metabolism</topic><topic>Wounds and Injuries - physiopathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matthies, Annette M.</creatorcontrib><creatorcontrib>Low, Quentin E.H.</creatorcontrib><creatorcontrib>Lingen, Mark W.</creatorcontrib><creatorcontrib>DiPietro, Luisa A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The American journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matthies, Annette M.</au><au>Low, Quentin E.H.</au><au>Lingen, Mark W.</au><au>DiPietro, Luisa A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neuropilin-1 Participates in Wound Angiogenesis</atitle><jtitle>The American journal of pathology</jtitle><addtitle>Am J Pathol</addtitle><date>2002</date><risdate>2002</risdate><volume>160</volume><issue>1</issue><spage>289</spage><epage>296</epage><pages>289-296</pages><issn>0002-9440</issn><eissn>1525-2191</eissn><coden>AJPAA4</coden><abstract>Angiogenesis, the formation of new capillaries from existing vasculature, plays an essential role in tissue repair. The rapid onset and predominance of proangiogenic factors optimizes healing in damaged tissues. One factor that directly mediates wound vessel angiogenesis is vascular endothelial growth factor (VEGF). Although much is known about the biology of VEGF and its cognate receptors, VEGFR1 and VEGFR2, the role of a recently identified co-receptor for VEGF, neuropilin-1, is not well understood. Using a murine model of dermal wound repair, we found that neuropilin-1 was abundantly expressed on new vasculature in healing wounds. Moreover, mice treated with anti-neuropilin-1 antibodies exhibited a significant decrease in vascular density within these wounds (67% decrease,
P = 0.0132). In
in vitro assays, VEGF induced formation of endothelial cord-like structures on collagen gel and endothelial cell migration toward VEGF was inhibited by antibodies directed against neuropilin-1. These results provide both
in vitro and
in vivo evidence for a critical role of neuropilin-1 in wound angiogenesis.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>11786422</pmid><doi>10.1016/S0002-9440(10)64372-6</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Biological and medical sciences Cells, Cultured Degeneration. Regeneration. Wound healing. Graft Endothelial Growth Factors - genetics Female Fundamental and applied biological sciences. Psychology Immunohistochemistry Lymphokines - genetics Mice Mice, Inbred BALB C Neovascularization, Physiologic - physiology Nerve Tissue Proteins - physiology Neuropilin-1 Protein Isoforms - genetics Receptor Protein-Tyrosine Kinases - genetics Receptors, Growth Factor - genetics Receptors, Vascular Endothelial Growth Factor Regular RNA, Messenger - metabolism Skin - injuries Tissue Distribution Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors Vertebrates: anatomy and physiology, studies on body, several organs or systems Wound Healing - physiology Wounds and Injuries - metabolism Wounds and Injuries - physiopathology |
| title | Neuropilin-1 Participates in Wound Angiogenesis |
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