Neuropilin-1 Participates in Wound Angiogenesis

Angiogenesis, the formation of new capillaries from existing vasculature, plays an essential role in tissue repair. The rapid onset and predominance of proangiogenic factors optimizes healing in damaged tissues. One factor that directly mediates wound vessel angiogenesis is vascular endothelial grow...

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Veröffentlicht in:The American journal of pathology 2002, Vol.160 (1), p.289-296
Hauptverfasser: Matthies, Annette M., Low, Quentin E.H., Lingen, Mark W., DiPietro, Luisa A.
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container_start_page 289
container_title The American journal of pathology
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creator Matthies, Annette M.
Low, Quentin E.H.
Lingen, Mark W.
DiPietro, Luisa A.
description Angiogenesis, the formation of new capillaries from existing vasculature, plays an essential role in tissue repair. The rapid onset and predominance of proangiogenic factors optimizes healing in damaged tissues. One factor that directly mediates wound vessel angiogenesis is vascular endothelial growth factor (VEGF). Although much is known about the biology of VEGF and its cognate receptors, VEGFR1 and VEGFR2, the role of a recently identified co-receptor for VEGF, neuropilin-1, is not well understood. Using a murine model of dermal wound repair, we found that neuropilin-1 was abundantly expressed on new vasculature in healing wounds. Moreover, mice treated with anti-neuropilin-1 antibodies exhibited a significant decrease in vascular density within these wounds (67% decrease, P = 0.0132). In in vitro assays, VEGF induced formation of endothelial cord-like structures on collagen gel and endothelial cell migration toward VEGF was inhibited by antibodies directed against neuropilin-1. These results provide both in vitro and in vivo evidence for a critical role of neuropilin-1 in wound angiogenesis.
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subjects Animals
Biological and medical sciences
Cells, Cultured
Degeneration. Regeneration. Wound healing. Graft
Endothelial Growth Factors - genetics
Female
Fundamental and applied biological sciences. Psychology
Immunohistochemistry
Lymphokines - genetics
Mice
Mice, Inbred BALB C
Neovascularization, Physiologic - physiology
Nerve Tissue Proteins - physiology
Neuropilin-1
Protein Isoforms - genetics
Receptor Protein-Tyrosine Kinases - genetics
Receptors, Growth Factor - genetics
Receptors, Vascular Endothelial Growth Factor
Regular
RNA, Messenger - metabolism
Skin - injuries
Tissue Distribution
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Wound Healing - physiology
Wounds and Injuries - metabolism
Wounds and Injuries - physiopathology
title Neuropilin-1 Participates in Wound Angiogenesis
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