Novel Short-Fragment PCR Assay for Highly Sensitive Broad-Spectrum Detection of Anogenital Human Papillomaviruses

A novel set of polymerase chain reaction (PCR) primers, designated SPF1 and SPF2 and located in the L1 region, was developed for universal detection of human papillomavirus (HPV). A short PCR fragment (SPF) of only 65 pb was synthesized. SPF amplimers were detected in a microtiter-based hybridizatio...

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Veröffentlicht in:	The American journal of pathology 1998-12, Vol.153 (6), p.1731-1739
Hauptverfasser:	Kleter, Bernhard, van Doorn, Leen-Jan, ter Schegget, Jan, Schrauwen, Lianne, van Krimpen, Kees, Burger, Matthé, ter Harmsel, Bram, Quint, Wim
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Blotting, Southern Cervix Uteri - virology Female Humans Investigative techniques, diagnostic techniques (general aspects) Medical sciences Miscellaneous. Technology Oligonucleotide Probes Papillomaviridae - isolation & purification Papillomavirus Infections - diagnosis Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase Chain Reaction - methods Precancerous Conditions - virology Sensitivity and Specificity Technical Advance Tumor Virus Infections - diagnosis Uterine Cervical Neoplasms - virology Vaginal Smears
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