Optimal processing of bone marrow trephine biopsy: the Hammersmith Protocol
Specimens of bone marrow trephine biopsy (BMT) are transported and fixed in acetic acid–zinc–formalin fixative, decalcified in 10% formic acid–5% formaldehyde and processed with other specimens to paraffin-wax embedding. Sections, 1-μm-thick, are cut by experienced histotechnologists and used for ha...
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| Veröffentlicht in: | Journal of clinical pathology 2006-09, Vol.59 (9), p.903-911 |
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| creator | Naresh, K N Lampert, I Hasserjian, R Lykidis, D Elderfield, K Horncastle, D Smith, N Murray-Brown, W Stamp, G W |
| description | Specimens of bone marrow trephine biopsy (BMT) are transported and fixed in acetic acid–zinc–formalin fixative, decalcified in 10% formic acid–5% formaldehyde and processed with other specimens to paraffin-wax embedding. Sections, 1-μm-thick, are cut by experienced histotechnologists and used for haematoxylin and eosin, Giemsa, reticulin silver and other histological stains. Further, all immunohistochemical procedures used in the laboratory, including double immunostaining, can be used on these sections with no or minimal modifications. About 10 000 BMT specimens have been analysed using this procedure since 1997 and diseases involving the bone marrow have been classified successfully. More recently, standardised polymerase chain reaction-based analysis and mRNA in situ hybridisation studies have been conducted. Excellent morphology with good antigen, DNA and RNA preservation is offered by the Hammersmith Protocol. |
| doi_str_mv | 10.1136/jcp.2004.020610 |
| format | Article |
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Sections, 1-μm-thick, are cut by experienced histotechnologists and used for haematoxylin and eosin, Giemsa, reticulin silver and other histological stains. Further, all immunohistochemical procedures used in the laboratory, including double immunostaining, can be used on these sections with no or minimal modifications. About 10 000 BMT specimens have been analysed using this procedure since 1997 and diseases involving the bone marrow have been classified successfully. More recently, standardised polymerase chain reaction-based analysis and mRNA in situ hybridisation studies have been conducted. Excellent morphology with good antigen, DNA and RNA preservation is offered by the Hammersmith Protocol.</description><identifier>ISSN: 0021-9746</identifier><identifier>EISSN: 1472-4146</identifier><identifier>DOI: 10.1136/jcp.2004.020610</identifier><identifier>PMID: 16935969</identifier><identifier>CODEN: JCPAAK</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and Association of Clinical Pathologists</publisher><subject>acetic acid–zinc–formalin ; Acids ; Antigens ; Automation ; AZF ; Biological and medical sciences ; Biopsy ; Biopsy - methods ; BMT ; Bone marrow ; Bone Marrow - pathology ; bone marrow biopsy using trephine ; Bone Marrow Examination - methods ; Bone Marrow Examination - standards ; Clinical Protocols ; DNA, Neoplasm - analysis ; EBV ; EDTA ; Epstein–Barr virus ; ethylene diamine tetra acetate ; H&E ; haematoxylin and eosin ; Hematology ; Histopathology ; Humans ; in situ hybridisation ; Investigative techniques, diagnostic techniques (general aspects) ; ISH ; Laboratories ; Medical sciences ; Methods ; Morphology ; My Approach ; Pathology. 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Miscellaneous investigative techniques ; Polymerase Chain Reaction - methods ; Protocol ; Staining and Labeling - methods ; Tissue Fixation - methods</subject><ispartof>Journal of clinical pathology, 2006-09, Vol.59 (9), p.903-911</ispartof><rights>Copyright 2006 Journal of Clinical Pathology</rights><rights>2006 INIST-CNRS</rights><rights>Copyright: 2006 Copyright 2006 Journal of Clinical Pathology</rights><rights>Copyright © 2006 The BMJ Publishing Group and the Association of Clinical Pathologists</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b522t-d43df22d580b6253fc93b4bf360dbe7de729042d38abdf5ee3df71aada897d953</citedby><cites>FETCH-LOGICAL-b522t-d43df22d580b6253fc93b4bf360dbe7de729042d38abdf5ee3df71aada897d953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://jcp.bmj.com/content/59/9/903.full.pdf$$EPDF$$P50$$Gbmj$$H</linktopdf><linktohtml>$$Uhttps://jcp.bmj.com/content/59/9/903.full$$EHTML$$P50$$Gbmj$$H</linktohtml><link.rule.ids>114,115,230,314,723,776,780,881,3183,23550,27901,27902,53766,53768,77343,77374</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18079054$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16935969$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Naresh, K N</creatorcontrib><creatorcontrib>Lampert, I</creatorcontrib><creatorcontrib>Hasserjian, R</creatorcontrib><creatorcontrib>Lykidis, D</creatorcontrib><creatorcontrib>Elderfield, K</creatorcontrib><creatorcontrib>Horncastle, D</creatorcontrib><creatorcontrib>Smith, N</creatorcontrib><creatorcontrib>Murray-Brown, W</creatorcontrib><creatorcontrib>Stamp, G W</creatorcontrib><title>Optimal processing of bone marrow trephine biopsy: the Hammersmith Protocol</title><title>Journal of clinical pathology</title><addtitle>J Clin Pathol</addtitle><description>Specimens of bone marrow trephine biopsy (BMT) are transported and fixed in acetic acid–zinc–formalin fixative, decalcified in 10% formic acid–5% formaldehyde and processed with other specimens to paraffin-wax embedding. Sections, 1-μm-thick, are cut by experienced histotechnologists and used for haematoxylin and eosin, Giemsa, reticulin silver and other histological stains. Further, all immunohistochemical procedures used in the laboratory, including double immunostaining, can be used on these sections with no or minimal modifications. About 10 000 BMT specimens have been analysed using this procedure since 1997 and diseases involving the bone marrow have been classified successfully. More recently, standardised polymerase chain reaction-based analysis and mRNA in situ hybridisation studies have been conducted. Excellent morphology with good antigen, DNA and RNA preservation is offered by the Hammersmith Protocol.</description><subject>acetic acid–zinc–formalin</subject><subject>Acids</subject><subject>Antigens</subject><subject>Automation</subject><subject>AZF</subject><subject>Biological and medical sciences</subject><subject>Biopsy</subject><subject>Biopsy - methods</subject><subject>BMT</subject><subject>Bone marrow</subject><subject>Bone Marrow - pathology</subject><subject>bone marrow biopsy using trephine</subject><subject>Bone Marrow Examination - methods</subject><subject>Bone Marrow Examination - standards</subject><subject>Clinical Protocols</subject><subject>DNA, Neoplasm - analysis</subject><subject>EBV</subject><subject>EDTA</subject><subject>Epstein–Barr virus</subject><subject>ethylene diamine tetra acetate</subject><subject>H&E</subject><subject>haematoxylin and eosin</subject><subject>Hematology</subject><subject>Histopathology</subject><subject>Humans</subject><subject>in situ hybridisation</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>ISH</subject><subject>Laboratories</subject><subject>Medical sciences</subject><subject>Methods</subject><subject>Morphology</subject><subject>My Approach</subject><subject>Pathology. 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| subjects | acetic acid–zinc–formalin Acids Antigens Automation AZF Biological and medical sciences Biopsy Biopsy - methods BMT Bone marrow Bone Marrow - pathology bone marrow biopsy using trephine Bone Marrow Examination - methods Bone Marrow Examination - standards Clinical Protocols DNA, Neoplasm - analysis EBV EDTA Epstein–Barr virus ethylene diamine tetra acetate H&E haematoxylin and eosin Hematology Histopathology Humans in situ hybridisation Investigative techniques, diagnostic techniques (general aspects) ISH Laboratories Medical sciences Methods Morphology My Approach Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase Chain Reaction - methods Protocol Staining and Labeling - methods Tissue Fixation - methods |
| title | Optimal processing of bone marrow trephine biopsy: the Hammersmith Protocol |
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