Serial transplantation reveals the stem-cell-like regenerative potential of adult mouse hepatocytes
Previous work has shown that adult mouse hepatocytes can divide at least 18 times in vivo. To test whether this represents the upper limit of their regenerative capacity, we performed serial transplantation of hepatocytes in the fumarylacetoacetate hydrolase deficiency murine model of liver repopula...
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| Veröffentlicht in: | The American journal of pathology 1997-11, Vol.151 (5), p.1273-1280 |
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| creator | Overturf, K al-Dhalimy, M Ou, CN Finegold, M Grompe, M |
| description | Previous work has shown that adult mouse hepatocytes can divide at least 18 times in vivo. To test whether this represents the upper limit of their regenerative capacity, we performed serial transplantation of hepatocytes in the fumarylacetoacetate hydrolase deficiency murine model of liver repopulation. Hepatocytes from adult donors were serially transplanted in limiting numbers six times and resulted in complete repopulation during each cycle. This corresponds to a minimal number of 69 cell doublings or a 7.3 x 10(20)-fold expansion. No evidence for abnormal liver function or altered hepatic architecture was found in repopulated animals. We conclude that a fraction of adult mouse hepatocytes have growth potential similar to that of hematopoietic stem cells. |
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To test whether this represents the upper limit of their regenerative capacity, we performed serial transplantation of hepatocytes in the fumarylacetoacetate hydrolase deficiency murine model of liver repopulation. Hepatocytes from adult donors were serially transplanted in limiting numbers six times and resulted in complete repopulation during each cycle. This corresponds to a minimal number of 69 cell doublings or a 7.3 x 10(20)-fold expansion. No evidence for abnormal liver function or altered hepatic architecture was found in repopulated animals. We conclude that a fraction of adult mouse hepatocytes have growth potential similar to that of hematopoietic stem cells.</description><identifier>ISSN: 0002-9440</identifier><identifier>EISSN: 1525-2191</identifier><identifier>PMID: 9358753</identifier><identifier>CODEN: AJPAA4</identifier><language>eng</language><publisher>Bethesda, MD: ASIP</publisher><subject>Animals ; Biological and medical sciences ; Cell Transformation, Neoplastic ; Cell Transplantation ; Degeneration. Regeneration. Wound healing. Graft ; Fundamental and applied biological sciences. 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To test whether this represents the upper limit of their regenerative capacity, we performed serial transplantation of hepatocytes in the fumarylacetoacetate hydrolase deficiency murine model of liver repopulation. Hepatocytes from adult donors were serially transplanted in limiting numbers six times and resulted in complete repopulation during each cycle. This corresponds to a minimal number of 69 cell doublings or a 7.3 x 10(20)-fold expansion. No evidence for abnormal liver function or altered hepatic architecture was found in repopulated animals. We conclude that a fraction of adult mouse hepatocytes have growth potential similar to that of hematopoietic stem cells.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Transformation, Neoplastic</subject><subject>Cell Transplantation</subject><subject>Degeneration. Regeneration. Wound healing. Graft</subject><subject>Fundamental and applied biological sciences. 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Regeneration. Wound healing. Graft</topic><topic>Fundamental and applied biological sciences. 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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Biological and medical sciences Cell Transformation, Neoplastic Cell Transplantation Degeneration. Regeneration. Wound healing. Graft Fundamental and applied biological sciences. Psychology Liver - cytology Liver - physiology Liver Regeneration - physiology Mice Mice, Mutant Strains Stem Cells - physiology Tissue Donors Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Serial transplantation reveals the stem-cell-like regenerative potential of adult mouse hepatocytes |
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