Nitric oxide synthase is expressed in human macrophages during foreign body inflammation
Although nitric oxide (NO) is a well documented effector molecule in rodent macrophages, its significance in human mononuclear phagocytic cells has been controversial. The foreign body inflammatory reaction around loosened joint replacement implants leads to formation of an osteolytic granulomatous...
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| Veröffentlicht in: | The American journal of pathology 1997-03, Vol.150 (3), p.881-887 |
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| creator | Moilanen, E Moilanen, T Knowles, R Charles, I Kadoya, Y al-Saffar, N Revell, PA Moncada, S |
| description | Although nitric oxide (NO) is a well documented effector molecule in rodent macrophages, its significance in human mononuclear phagocytic cells has been controversial. The foreign body inflammatory reaction around loosened joint replacement implants leads to formation of an osteolytic granulomatous pseudo-synovial membrane rich in activated macrophages. We studied 13 specimens of interface membrane tissue collected from revision surgery of aseptically loosened hip and knee prostheses for the presence of inducible NO synthase (iNOS). The presence of iNOS was demonstrated immunohistochemically in 10 of these specimens. Within the tissue this enzyme was confined to macrophages and vascular endothelial cells. iNOS activity was demonstrated biochemically by measuring the calcium-independent generation of citrulline from L-arginine, and the presence of iNOS mRNA was demonstrated using reverse transcriptase polymerase chain reaction. NO synthesis in the interface tissue may be an important factor in the maintenance of the inflammatory and osteolytic processes. |
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The foreign body inflammatory reaction around loosened joint replacement implants leads to formation of an osteolytic granulomatous pseudo-synovial membrane rich in activated macrophages. We studied 13 specimens of interface membrane tissue collected from revision surgery of aseptically loosened hip and knee prostheses for the presence of inducible NO synthase (iNOS). The presence of iNOS was demonstrated immunohistochemically in 10 of these specimens. Within the tissue this enzyme was confined to macrophages and vascular endothelial cells. iNOS activity was demonstrated biochemically by measuring the calcium-independent generation of citrulline from L-arginine, and the presence of iNOS mRNA was demonstrated using reverse transcriptase polymerase chain reaction. NO synthesis in the interface tissue may be an important factor in the maintenance of the inflammatory and osteolytic processes.</description><identifier>ISSN: 0002-9440</identifier><identifier>EISSN: 1525-2191</identifier><identifier>PMID: 9060826</identifier><identifier>CODEN: AJPAA4</identifier><language>eng</language><publisher>Bethesda, MD: ASIP</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Antibodies - analysis ; Base Sequence ; Biological and medical sciences ; Foreign-Body Reaction - metabolism ; Foreign-Body Reaction - pathology ; Humans ; Immunohistochemistry ; Joint Prosthesis ; Macrophage Activation ; Macrophages - enzymology ; Medical sciences ; Middle Aged ; Nitric Oxide Synthase - biosynthesis ; Orthopedic surgery ; Polymerase Chain Reaction ; Prosthesis Failure ; Reoperation ; RNA - isolation & purification ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Synovial Membrane - chemistry ; Synovial Membrane - pathology</subject><ispartof>The American journal of pathology, 1997-03, Vol.150 (3), p.881-887</ispartof><rights>1997 INIST-CNRS</rights><rights>Copyright American Society for Investigative Pathology Mar 1997</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1857881/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1857881/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2600268$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9060826$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moilanen, E</creatorcontrib><creatorcontrib>Moilanen, T</creatorcontrib><creatorcontrib>Knowles, R</creatorcontrib><creatorcontrib>Charles, I</creatorcontrib><creatorcontrib>Kadoya, Y</creatorcontrib><creatorcontrib>al-Saffar, N</creatorcontrib><creatorcontrib>Revell, PA</creatorcontrib><creatorcontrib>Moncada, S</creatorcontrib><title>Nitric oxide synthase is expressed in human macrophages during foreign body inflammation</title><title>The American journal of pathology</title><addtitle>Am J Pathol</addtitle><description>Although nitric oxide (NO) is a well documented effector molecule in rodent macrophages, its significance in human mononuclear phagocytic cells has been controversial. The foreign body inflammatory reaction around loosened joint replacement implants leads to formation of an osteolytic granulomatous pseudo-synovial membrane rich in activated macrophages. We studied 13 specimens of interface membrane tissue collected from revision surgery of aseptically loosened hip and knee prostheses for the presence of inducible NO synthase (iNOS). The presence of iNOS was demonstrated immunohistochemically in 10 of these specimens. Within the tissue this enzyme was confined to macrophages and vascular endothelial cells. iNOS activity was demonstrated biochemically by measuring the calcium-independent generation of citrulline from L-arginine, and the presence of iNOS mRNA was demonstrated using reverse transcriptase polymerase chain reaction. NO synthesis in the interface tissue may be an important factor in the maintenance of the inflammatory and osteolytic processes.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antibodies - analysis</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Foreign-Body Reaction - metabolism</subject><subject>Foreign-Body Reaction - pathology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Joint Prosthesis</subject><subject>Macrophage Activation</subject><subject>Macrophages - enzymology</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Nitric Oxide Synthase - biosynthesis</subject><subject>Orthopedic surgery</subject><subject>Polymerase Chain Reaction</subject><subject>Prosthesis Failure</subject><subject>Reoperation</subject><subject>RNA - isolation & purification</subject><subject>Surgery (general aspects). 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The foreign body inflammatory reaction around loosened joint replacement implants leads to formation of an osteolytic granulomatous pseudo-synovial membrane rich in activated macrophages. We studied 13 specimens of interface membrane tissue collected from revision surgery of aseptically loosened hip and knee prostheses for the presence of inducible NO synthase (iNOS). The presence of iNOS was demonstrated immunohistochemically in 10 of these specimens. Within the tissue this enzyme was confined to macrophages and vascular endothelial cells. iNOS activity was demonstrated biochemically by measuring the calcium-independent generation of citrulline from L-arginine, and the presence of iNOS mRNA was demonstrated using reverse transcriptase polymerase chain reaction. NO synthesis in the interface tissue may be an important factor in the maintenance of the inflammatory and osteolytic processes.</abstract><cop>Bethesda, MD</cop><pub>ASIP</pub><pmid>9060826</pmid><tpages>7</tpages></addata></record> |
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| ispartof | The American journal of pathology, 1997-03, Vol.150 (3), p.881-887 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Adult Aged Aged, 80 and over Antibodies - analysis Base Sequence Biological and medical sciences Foreign-Body Reaction - metabolism Foreign-Body Reaction - pathology Humans Immunohistochemistry Joint Prosthesis Macrophage Activation Macrophages - enzymology Medical sciences Middle Aged Nitric Oxide Synthase - biosynthesis Orthopedic surgery Polymerase Chain Reaction Prosthesis Failure Reoperation RNA - isolation & purification Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Synovial Membrane - chemistry Synovial Membrane - pathology |
| title | Nitric oxide synthase is expressed in human macrophages during foreign body inflammation |
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