Immunohistochemical localization of defensin in human coronary vessels

Neutrophil defensins comprise a family of cationic peptides that possess potent antimicrobial activity. Defensins are normally sequestered in cytoplasmic granules with their primary site of action in phagolysosomes, although some peptide is released into the circulation during the course of infectio...

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Veröffentlicht in:The American journal of pathology 1997-03, Vol.150 (3), p.1009-1020
Hauptverfasser: Barnathan, ES, Raghunath, PN, Tomaszewski, JE, Ganz, T, Cines, DB, Higazi A, al-R
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container_end_page 1020
container_issue 3
container_start_page 1009
container_title The American journal of pathology
container_volume 150
creator Barnathan, ES
Raghunath, PN
Tomaszewski, JE
Ganz, T
Cines, DB
Higazi A, al-R
description Neutrophil defensins comprise a family of cationic peptides that possess potent antimicrobial activity. Defensins are normally sequestered in cytoplasmic granules with their primary site of action in phagolysosomes, although some peptide is released into the circulation during the course of infection or inflammation. In view of the fact that neutrophils adhere to the endothelium and that defensins have been reported to bind to human endothelial cells in vitro, we used immunohistochemistry to study the distribution of these peptides in normal and in atherosclerotic human coronary arteries. Defensin was found primarily in the intima of normal and atherosclerotic vessels, most prominently in association with intimal smooth muscle cells. Both large- and small-vessel endothelium stained focally for defensin. Defensin was also found in the media near the external elastic lamina and in some periadventitial vessels. The same distribution was seen in vessels that had been perfusion fixed immediately upon procurement, excluding diffusion of defensin from PMNs ex vivo. These data indicate that neutrophil defensin is present in the walls of human coronary arteries. The deposition of defensin in vessels may contribute to the pathophysiological consequences of inflammation in addition to their role in host defense.
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Defensins are normally sequestered in cytoplasmic granules with their primary site of action in phagolysosomes, although some peptide is released into the circulation during the course of infection or inflammation. In view of the fact that neutrophils adhere to the endothelium and that defensins have been reported to bind to human endothelial cells in vitro, we used immunohistochemistry to study the distribution of these peptides in normal and in atherosclerotic human coronary arteries. Defensin was found primarily in the intima of normal and atherosclerotic vessels, most prominently in association with intimal smooth muscle cells. Both large- and small-vessel endothelium stained focally for defensin. Defensin was also found in the media near the external elastic lamina and in some periadventitial vessels. The same distribution was seen in vessels that had been perfusion fixed immediately upon procurement, excluding diffusion of defensin from PMNs ex vivo. These data indicate that neutrophil defensin is present in the walls of human coronary arteries. The deposition of defensin in vessels may contribute to the pathophysiological consequences of inflammation in addition to their role in host defense.</abstract><cop>Bethesda, MD</cop><pub>ASIP</pub><pmid>9060838</pmid><tpages>12</tpages></addata></record>
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subjects Anti-Infective Agents - analysis
Anti-Infective Agents - metabolism
Antibodies - analysis
Arteriosclerosis - pathology
Arteriosclerosis - physiopathology
Atherosclerosis (general aspects, experimental research)
Biological and medical sciences
Blood and lymphatic vessels
Blood Proteins - analysis
Blood Proteins - immunology
Blood Proteins - metabolism
Cardiology. Vascular system
Coronary Vessels - chemistry
Coronary Vessels - metabolism
Defensins
Endothelium, Vascular - chemistry
Female
Fibrinolysis - physiology
Humans
Immunohistochemistry
Male
Medical sciences
Reference Values
Tunica Intima - chemistry
Tunica Intima - cytology
Tunica Media - chemistry
Tunica Media - cytology
title Immunohistochemical localization of defensin in human coronary vessels
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