Pattern of nonspecific (or global) DNA methylation in oral carcinogenesis
Background. Although alterations in nonspecific (or global) DNA methylation (GDM) in specific cells are known to be involved in the process of lung carcinogenesis, similar associations have not been evaluated in other smoking‐related cancers of the head and neck. Methods. We evaluated the status of...
Gespeichert in:
| Veröffentlicht in: | Head & neck 2005-12, Vol.27 (12), p.1061-1067 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1067 |
|---|---|
| container_issue | 12 |
| container_start_page | 1061 |
| container_title | Head & neck |
| container_volume | 27 |
| creator | Piyathilake, Chandrika J. Bell, Walter C. Jones, Jennifer Henao, Olga L. Heimburger, Douglas C. Niveleau, Alain Grizzle, William E. |
| description | Background.
Although alterations in nonspecific (or global) DNA methylation (GDM) in specific cells are known to be involved in the process of lung carcinogenesis, similar associations have not been evaluated in other smoking‐related cancers of the head and neck.
Methods.
We evaluated the status of GDM by using monoclonal antibodies specific for 5‐methylcytosine (5‐mc) in oral squamous cell carcinoma (SCC) specimens of 48 cigarette smokers who had SCC develop and in 93 age‐, race‐, and sex‐matched smokers who did not.
Results.
Percentages of cells positive for 5‐mc immunostaining of DNA of SCC and dysplastic lesions were significantly higher than those of normal oral epithelial cells from cancer subjects and from noncancer subjects. The degree of DNA methylation was unrelated to DNA content.
Conclusions.
The pattern of GDM in oral SCCs is different from that of lung SCCs. The differences in nutrient risk factor profiles that are related to GDM and differential activity of DNA methyltranferases between oral and lung SCCs may explain these observations. © 2005 Wiley Periodicals, Inc. Head Neck 27: XXX–XXX, 2005 |
| doi_str_mv | 10.1002/hed.20288 |
| format | Article |
| fullrecord | <record><control><sourceid>wiley_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1853326</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>HED20288</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4818-97e62eb613032d58b889ddf1021a87d22fbd79e7e364b23b9c28d23c867c5e253</originalsourceid><addsrcrecordid>eNp1kElPwzAQhS0EYj_wB1AuSHAIeEli-4JUKBQkVEACcbQcZ9IaUruyw9J_T6BlO3CakeZ7740eQjsEHxKM6dEYqkOKqRBLaJ1gyVPMMr78sWcsZZhna2gjxkeMMSsyuorWSEHyXBK-ji5vdNtCcImvE-ddnIKxtTXJvg_JqPGlbg6S_rCXTKAdzxrdWu8S29FBN4nRwVjnR-Ag2riFVmrdRNhezE10f352d3qRXl0PLk97V6nJBBGp5FBQKAvCMKNVLkohZFXVBFOiBa8orcuKS-DQvVpSVkpDRUWZEQU3OdCcbaLjue_0uZxAZcC13TNqGuxEh5ny2qq_F2fHauRfFBE5Y7ToDA7mBib4GAPU31qC1UefqutTffbZsbu_w37IRYEdsLcAdDS6qYN2xsYfjjOSSyE77mjOvdoGZv8nqouz_ld0OlfY2MLbt0KHJ1VwxnP1MByovmTD28EJUXfsHYXVm30</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Pattern of nonspecific (or global) DNA methylation in oral carcinogenesis</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Piyathilake, Chandrika J. ; Bell, Walter C. ; Jones, Jennifer ; Henao, Olga L. ; Heimburger, Douglas C. ; Niveleau, Alain ; Grizzle, William E.</creator><creatorcontrib>Piyathilake, Chandrika J. ; Bell, Walter C. ; Jones, Jennifer ; Henao, Olga L. ; Heimburger, Douglas C. ; Niveleau, Alain ; Grizzle, William E.</creatorcontrib><description>Background.
Although alterations in nonspecific (or global) DNA methylation (GDM) in specific cells are known to be involved in the process of lung carcinogenesis, similar associations have not been evaluated in other smoking‐related cancers of the head and neck.
Methods.
We evaluated the status of GDM by using monoclonal antibodies specific for 5‐methylcytosine (5‐mc) in oral squamous cell carcinoma (SCC) specimens of 48 cigarette smokers who had SCC develop and in 93 age‐, race‐, and sex‐matched smokers who did not.
Results.
Percentages of cells positive for 5‐mc immunostaining of DNA of SCC and dysplastic lesions were significantly higher than those of normal oral epithelial cells from cancer subjects and from noncancer subjects. The degree of DNA methylation was unrelated to DNA content.
Conclusions.
The pattern of GDM in oral SCCs is different from that of lung SCCs. The differences in nutrient risk factor profiles that are related to GDM and differential activity of DNA methyltranferases between oral and lung SCCs may explain these observations. © 2005 Wiley Periodicals, Inc. Head Neck 27: XXX–XXX, 2005</description><identifier>ISSN: 1043-3074</identifier><identifier>EISSN: 1097-0347</identifier><identifier>DOI: 10.1002/hed.20288</identifier><identifier>PMID: 16155917</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>5-Methylcytosine - immunology ; Antibodies, Monoclonal - immunology ; Biological and medical sciences ; Carcinoma, Squamous Cell - genetics ; Carcinoma, Squamous Cell - pathology ; Case-Control Studies ; DNA ; DNA Methylation ; Epithelium - pathology ; Humans ; Immunohistochemistry ; Medical sciences ; methylation ; Mouth Mucosa - pathology ; Mouth Neoplasms - genetics ; Mouth Neoplasms - pathology ; oral cancer ; Otorhinolaryngology. Stomatology ; Precancerous Conditions - genetics ; Precancerous Conditions - pathology ; Smoking - epidemiology ; Tumors ; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><ispartof>Head & neck, 2005-12, Vol.27 (12), p.1061-1067</ispartof><rights>Copyright © 2005 Wiley Periodicals, Inc.</rights><rights>2006 INIST-CNRS</rights><rights>(c) 2005 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4818-97e62eb613032d58b889ddf1021a87d22fbd79e7e364b23b9c28d23c867c5e253</citedby><cites>FETCH-LOGICAL-c4818-97e62eb613032d58b889ddf1021a87d22fbd79e7e364b23b9c28d23c867c5e253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fhed.20288$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fhed.20288$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17315989$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16155917$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Piyathilake, Chandrika J.</creatorcontrib><creatorcontrib>Bell, Walter C.</creatorcontrib><creatorcontrib>Jones, Jennifer</creatorcontrib><creatorcontrib>Henao, Olga L.</creatorcontrib><creatorcontrib>Heimburger, Douglas C.</creatorcontrib><creatorcontrib>Niveleau, Alain</creatorcontrib><creatorcontrib>Grizzle, William E.</creatorcontrib><title>Pattern of nonspecific (or global) DNA methylation in oral carcinogenesis</title><title>Head & neck</title><addtitle>Head Neck</addtitle><description>Background.
Although alterations in nonspecific (or global) DNA methylation (GDM) in specific cells are known to be involved in the process of lung carcinogenesis, similar associations have not been evaluated in other smoking‐related cancers of the head and neck.
Methods.
We evaluated the status of GDM by using monoclonal antibodies specific for 5‐methylcytosine (5‐mc) in oral squamous cell carcinoma (SCC) specimens of 48 cigarette smokers who had SCC develop and in 93 age‐, race‐, and sex‐matched smokers who did not.
Results.
Percentages of cells positive for 5‐mc immunostaining of DNA of SCC and dysplastic lesions were significantly higher than those of normal oral epithelial cells from cancer subjects and from noncancer subjects. The degree of DNA methylation was unrelated to DNA content.
Conclusions.
The pattern of GDM in oral SCCs is different from that of lung SCCs. The differences in nutrient risk factor profiles that are related to GDM and differential activity of DNA methyltranferases between oral and lung SCCs may explain these observations. © 2005 Wiley Periodicals, Inc. Head Neck 27: XXX–XXX, 2005</description><subject>5-Methylcytosine - immunology</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Case-Control Studies</subject><subject>DNA</subject><subject>DNA Methylation</subject><subject>Epithelium - pathology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medical sciences</subject><subject>methylation</subject><subject>Mouth Mucosa - pathology</subject><subject>Mouth Neoplasms - genetics</subject><subject>Mouth Neoplasms - pathology</subject><subject>oral cancer</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Precancerous Conditions - genetics</subject><subject>Precancerous Conditions - pathology</subject><subject>Smoking - epidemiology</subject><subject>Tumors</subject><subject>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><issn>1043-3074</issn><issn>1097-0347</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kElPwzAQhS0EYj_wB1AuSHAIeEli-4JUKBQkVEACcbQcZ9IaUruyw9J_T6BlO3CakeZ7740eQjsEHxKM6dEYqkOKqRBLaJ1gyVPMMr78sWcsZZhna2gjxkeMMSsyuorWSEHyXBK-ji5vdNtCcImvE-ddnIKxtTXJvg_JqPGlbg6S_rCXTKAdzxrdWu8S29FBN4nRwVjnR-Ag2riFVmrdRNhezE10f352d3qRXl0PLk97V6nJBBGp5FBQKAvCMKNVLkohZFXVBFOiBa8orcuKS-DQvVpSVkpDRUWZEQU3OdCcbaLjue_0uZxAZcC13TNqGuxEh5ny2qq_F2fHauRfFBE5Y7ToDA7mBib4GAPU31qC1UefqutTffbZsbu_w37IRYEdsLcAdDS6qYN2xsYfjjOSSyE77mjOvdoGZv8nqouz_ld0OlfY2MLbt0KHJ1VwxnP1MByovmTD28EJUXfsHYXVm30</recordid><startdate>200512</startdate><enddate>200512</enddate><creator>Piyathilake, Chandrika J.</creator><creator>Bell, Walter C.</creator><creator>Jones, Jennifer</creator><creator>Henao, Olga L.</creator><creator>Heimburger, Douglas C.</creator><creator>Niveleau, Alain</creator><creator>Grizzle, William E.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>John Wiley & Sons</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>200512</creationdate><title>Pattern of nonspecific (or global) DNA methylation in oral carcinogenesis</title><author>Piyathilake, Chandrika J. ; Bell, Walter C. ; Jones, Jennifer ; Henao, Olga L. ; Heimburger, Douglas C. ; Niveleau, Alain ; Grizzle, William E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4818-97e62eb613032d58b889ddf1021a87d22fbd79e7e364b23b9c28d23c867c5e253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>5-Methylcytosine - immunology</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Squamous Cell - genetics</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Case-Control Studies</topic><topic>DNA</topic><topic>DNA Methylation</topic><topic>Epithelium - pathology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Medical sciences</topic><topic>methylation</topic><topic>Mouth Mucosa - pathology</topic><topic>Mouth Neoplasms - genetics</topic><topic>Mouth Neoplasms - pathology</topic><topic>oral cancer</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Precancerous Conditions - genetics</topic><topic>Precancerous Conditions - pathology</topic><topic>Smoking - epidemiology</topic><topic>Tumors</topic><topic>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Piyathilake, Chandrika J.</creatorcontrib><creatorcontrib>Bell, Walter C.</creatorcontrib><creatorcontrib>Jones, Jennifer</creatorcontrib><creatorcontrib>Henao, Olga L.</creatorcontrib><creatorcontrib>Heimburger, Douglas C.</creatorcontrib><creatorcontrib>Niveleau, Alain</creatorcontrib><creatorcontrib>Grizzle, William E.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Head & neck</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Piyathilake, Chandrika J.</au><au>Bell, Walter C.</au><au>Jones, Jennifer</au><au>Henao, Olga L.</au><au>Heimburger, Douglas C.</au><au>Niveleau, Alain</au><au>Grizzle, William E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pattern of nonspecific (or global) DNA methylation in oral carcinogenesis</atitle><jtitle>Head & neck</jtitle><addtitle>Head Neck</addtitle><date>2005-12</date><risdate>2005</risdate><volume>27</volume><issue>12</issue><spage>1061</spage><epage>1067</epage><pages>1061-1067</pages><issn>1043-3074</issn><eissn>1097-0347</eissn><abstract>Background.
Although alterations in nonspecific (or global) DNA methylation (GDM) in specific cells are known to be involved in the process of lung carcinogenesis, similar associations have not been evaluated in other smoking‐related cancers of the head and neck.
Methods.
We evaluated the status of GDM by using monoclonal antibodies specific for 5‐methylcytosine (5‐mc) in oral squamous cell carcinoma (SCC) specimens of 48 cigarette smokers who had SCC develop and in 93 age‐, race‐, and sex‐matched smokers who did not.
Results.
Percentages of cells positive for 5‐mc immunostaining of DNA of SCC and dysplastic lesions were significantly higher than those of normal oral epithelial cells from cancer subjects and from noncancer subjects. The degree of DNA methylation was unrelated to DNA content.
Conclusions.
The pattern of GDM in oral SCCs is different from that of lung SCCs. The differences in nutrient risk factor profiles that are related to GDM and differential activity of DNA methyltranferases between oral and lung SCCs may explain these observations. © 2005 Wiley Periodicals, Inc. Head Neck 27: XXX–XXX, 2005</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>16155917</pmid><doi>10.1002/hed.20288</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1043-3074 |
| ispartof | Head & neck, 2005-12, Vol.27 (12), p.1061-1067 |
| issn | 1043-3074 1097-0347 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1853326 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | 5-Methylcytosine - immunology Antibodies, Monoclonal - immunology Biological and medical sciences Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - pathology Case-Control Studies DNA DNA Methylation Epithelium - pathology Humans Immunohistochemistry Medical sciences methylation Mouth Mucosa - pathology Mouth Neoplasms - genetics Mouth Neoplasms - pathology oral cancer Otorhinolaryngology. Stomatology Precancerous Conditions - genetics Precancerous Conditions - pathology Smoking - epidemiology Tumors Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology |
| title | Pattern of nonspecific (or global) DNA methylation in oral carcinogenesis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T13%3A09%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wiley_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pattern%20of%20nonspecific%20(or%20global)%20DNA%20methylation%20in%20oral%20carcinogenesis&rft.jtitle=Head%20&%20neck&rft.au=Piyathilake,%20Chandrika%20J.&rft.date=2005-12&rft.volume=27&rft.issue=12&rft.spage=1061&rft.epage=1067&rft.pages=1061-1067&rft.issn=1043-3074&rft.eissn=1097-0347&rft_id=info:doi/10.1002/hed.20288&rft_dat=%3Cwiley_pubme%3EHED20288%3C/wiley_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/16155917&rfr_iscdi=true |