Fis Targets Assembly of the Xis Nucleoprotein Filament to Promote Excisive Recombination by Phage Lambda

The phage-encoded Xis protein is the major determinant controlling the direction of recombination in phage lambda. Xis is a winged-helix DNA binding protein that cooperatively binds to the attR recombination site to generate a curved microfilament, which promotes assembly of the excisive intasome bu...

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Veröffentlicht in:	Journal of molecular biology 2007-03, Vol.367 (2), p.328-343
Hauptverfasser:	Papagiannis, Christie V., Sam, My D., Abbani, Mohamad A., Yoo, Daniel, Cascio, Duilio, Clubb, Robert T., Johnson, Reid C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - metabolism Bacteriophage lambda - genetics Bacteriophage lambda - metabolism Base Sequence Binding Sites Crystallography, X-Ray Dimerization DNA architectural proteins DNA bending DNA Nucleotidyltransferases - genetics DNA Nucleotidyltransferases - metabolism Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Factor For Inversion Stimulation Protein Membrane Proteins - metabolism Molecular Sequence Data Mutation nonspecific DNA binding Nucleoproteins - genetics Nucleoproteins - metabolism Promoter Regions, Genetic Protein Binding Protein Conformation Recombination, Genetic site-specific DNA recombination Transcription Factors - genetics Transcription Factors - metabolism Viral Proteins - genetics Viral Proteins - metabolism Virus Activation X-ray crystallography
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