Carp, a Cardiac Ankyrin-Repeated Protein, and Its New Homologue, Arpp, Are Differentially Expressed in Heart, Skeletal Muscle, and Rhabdomyosarcomas
Arpp, a protein containing an ankyrin repeat domain, PEST sequence, and proline-rich region, is a novel ankyrin-repeated protein highly homologous to Carp, which is proposed to be the putative genetic marker for cardiac hypertrophy. In this study, we comparatively analyzed expression of Arpp and Car...
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| Veröffentlicht in: | The American journal of pathology 2002-05, Vol.160 (5), p.1767-1778 |
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| creator | Ishiguro, Naoko Baba, Takeshi Ishida, Tsuyoshi Takeuchi, Kengo Osaki, Mitsuhiko Araki, Nobuhito Okada, Eikichi Takahashi, Satsuki Saito, Masahiro Watanabe, Mari Nakada, Chisato Tsukamoto, Yoshiyuki Sato, Kenzo Ito, Kinji Fukayama, Masashi Mori, Shigeo Ito, Hisao Moriyama, Masatsugu |
| description | Arpp, a protein containing an ankyrin repeat domain, PEST sequence, and proline-rich region, is a novel ankyrin-repeated protein highly homologous to Carp, which is proposed to be the putative genetic marker for cardiac hypertrophy. In this study, we comparatively analyzed expression of Arpp and Carp protein in skeletal and cardiac muscles and rhabdomyosarcomas (RMSs). In adult skeletal muscle, Arpp was preferentially expressed in the nucleus and cytoplasm of type I fibers, whereas Carp was barely detectable in skeletal muscle. On the other hand, in adult cardiac muscle, interestingly, Arpp was expressed in ventricles mostly, whereas Carp was expressed throughout the atrium and ventricle. Furthermore, although Carp was identified in fetal heart at 11 developmental weeks, Arpp was very low or undetectable in these fetal hearts. These results suggest that Arpp and Carp are differentially expressed and function in both skeletal and cardiac muscle of fetus and adult. We found that Arpp expression was induced during the differentiation of C2C12 cells
in vitro
, suggesting that Arpp-expression may be associated with the differentiation stage during myogenesis. Both Arpp and Carp were found to be expressed in all of the RMS cases studied. Because the expression patterns of Arpp in RMS were different from those of muscle actin or desmin, Arpp may be detectable in RMS cases that do not express other existing RMS markers. |
| doi_str_mv | 10.1016/S0002-9440(10)61123-6 |
| format | Article |
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in vitro
, suggesting that Arpp-expression may be associated with the differentiation stage during myogenesis. Both Arpp and Carp were found to be expressed in all of the RMS cases studied. Because the expression patterns of Arpp in RMS were different from those of muscle actin or desmin, Arpp may be detectable in RMS cases that do not express other existing RMS markers.</description><identifier>ISSN: 0002-9440</identifier><identifier>EISSN: 1525-2191</identifier><identifier>DOI: 10.1016/S0002-9440(10)61123-6</identifier><identifier>PMID: 12000728</identifier><identifier>CODEN: AJPAA4</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Actins - analysis ; Adolescent ; Adult ; Aged ; Amino Acid Sequence ; Animals ; Antibodies - immunology ; Antibody Specificity ; Biological and medical sciences ; Cell Differentiation ; Cell Line ; Child ; Desmin - analysis ; Diseases of the osteoarticular system ; Female ; Fetal Heart - chemistry ; HeLa Cells ; Humans ; Immunohistochemistry ; Leiomyosarcoma - metabolism ; Leiomyosarcoma - pathology ; Male ; Medical sciences ; Middle Aged ; Molecular Sequence Data ; Muscle Fibers, Slow-Twitch - chemistry ; Muscle Proteins ; Muscle, Skeletal - chemistry ; Muscle, Skeletal - cytology ; Muscle, Smooth - chemistry ; Myocardium - cytology ; Myocardium - metabolism ; Myoglobin - analysis ; Nuclear Proteins - biosynthesis ; Nuclear Proteins - genetics ; Nuclear Proteins - immunology ; Regular ; Repressor Proteins - biosynthesis ; Repressor Proteins - genetics ; Repressor Proteins - immunology ; Rhabdomyosarcoma - metabolism ; Rhabdomyosarcoma - pathology ; Sequence Homology, Amino Acid ; Tumors of striated muscle and skeleton</subject><ispartof>The American journal of pathology, 2002-05, Vol.160 (5), p.1767-1778</ispartof><rights>2002 American Society for Investigative Pathology</rights><rights>2002 INIST-CNRS</rights><rights>Copyright © 2002, American Society for Investigative Pathology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c591t-508c061c06cb850dbe4bc79c4318d70e5cb4e3b788cf7c8cae69a672401617e23</citedby><cites>FETCH-LOGICAL-c591t-508c061c06cb850dbe4bc79c4318d70e5cb4e3b788cf7c8cae69a672401617e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1850855/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0002-9440(10)61123-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3536,27903,27904,45974,53770,53772</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14169117$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12000728$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ishiguro, Naoko</creatorcontrib><creatorcontrib>Baba, Takeshi</creatorcontrib><creatorcontrib>Ishida, Tsuyoshi</creatorcontrib><creatorcontrib>Takeuchi, Kengo</creatorcontrib><creatorcontrib>Osaki, Mitsuhiko</creatorcontrib><creatorcontrib>Araki, Nobuhito</creatorcontrib><creatorcontrib>Okada, Eikichi</creatorcontrib><creatorcontrib>Takahashi, Satsuki</creatorcontrib><creatorcontrib>Saito, Masahiro</creatorcontrib><creatorcontrib>Watanabe, Mari</creatorcontrib><creatorcontrib>Nakada, Chisato</creatorcontrib><creatorcontrib>Tsukamoto, Yoshiyuki</creatorcontrib><creatorcontrib>Sato, Kenzo</creatorcontrib><creatorcontrib>Ito, Kinji</creatorcontrib><creatorcontrib>Fukayama, Masashi</creatorcontrib><creatorcontrib>Mori, Shigeo</creatorcontrib><creatorcontrib>Ito, Hisao</creatorcontrib><creatorcontrib>Moriyama, Masatsugu</creatorcontrib><title>Carp, a Cardiac Ankyrin-Repeated Protein, and Its New Homologue, Arpp, Are Differentially Expressed in Heart, Skeletal Muscle, and Rhabdomyosarcomas</title><title>The American journal of pathology</title><addtitle>Am J Pathol</addtitle><description>Arpp, a protein containing an ankyrin repeat domain, PEST sequence, and proline-rich region, is a novel ankyrin-repeated protein highly homologous to Carp, which is proposed to be the putative genetic marker for cardiac hypertrophy. In this study, we comparatively analyzed expression of Arpp and Carp protein in skeletal and cardiac muscles and rhabdomyosarcomas (RMSs). In adult skeletal muscle, Arpp was preferentially expressed in the nucleus and cytoplasm of type I fibers, whereas Carp was barely detectable in skeletal muscle. On the other hand, in adult cardiac muscle, interestingly, Arpp was expressed in ventricles mostly, whereas Carp was expressed throughout the atrium and ventricle. Furthermore, although Carp was identified in fetal heart at 11 developmental weeks, Arpp was very low or undetectable in these fetal hearts. These results suggest that Arpp and Carp are differentially expressed and function in both skeletal and cardiac muscle of fetus and adult. We found that Arpp expression was induced during the differentiation of C2C12 cells
in vitro
, suggesting that Arpp-expression may be associated with the differentiation stage during myogenesis. Both Arpp and Carp were found to be expressed in all of the RMS cases studied. Because the expression patterns of Arpp in RMS were different from those of muscle actin or desmin, Arpp may be detectable in RMS cases that do not express other existing RMS markers.</description><subject>Actins - analysis</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Child</subject><subject>Desmin - analysis</subject><subject>Diseases of the osteoarticular system</subject><subject>Female</subject><subject>Fetal Heart - chemistry</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Leiomyosarcoma - metabolism</subject><subject>Leiomyosarcoma - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Muscle Fibers, Slow-Twitch - chemistry</subject><subject>Muscle Proteins</subject><subject>Muscle, Skeletal - chemistry</subject><subject>Muscle, Skeletal - cytology</subject><subject>Muscle, Smooth - chemistry</subject><subject>Myocardium - cytology</subject><subject>Myocardium - metabolism</subject><subject>Myoglobin - analysis</subject><subject>Nuclear Proteins - biosynthesis</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - immunology</subject><subject>Regular</subject><subject>Repressor Proteins - biosynthesis</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - immunology</subject><subject>Rhabdomyosarcoma - metabolism</subject><subject>Rhabdomyosarcoma - pathology</subject><subject>Sequence Homology, Amino Acid</subject><subject>Tumors of striated muscle and skeleton</subject><issn>0002-9440</issn><issn>1525-2191</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdtu1DAQhiMEotvCI4B8AwVpA54kdpIb0GopbKVyUAvXluNMdt06cbCTln0PHhhvs2rhigt7fPjmn9H8UfQM6BugwN9eUEqTuMwy-groaw6QpDF_EM2AJSxOoISH0ewOOYgOvb8MV54W9HF0AEk450kxi34vpevnRJIQay0VWXRXW6e7-Bx7lAPW5JuzA-ouMF1NTgdPvuANWdnWGrsecU4Wru93O5IPumnQYTdoacyWnPzqHXofJHRHVijdMCcXV2hwkIZ8Hr0yOImeb2RV23ZrvXTKttI_iR410nh8uo9H0Y-PJ9-Xq_js66fT5eIsVqyEIWa0UJRDWKoqGK0rzCqVlypLoahzikxVGaZVXhSqyVWhJPJS8jzJwvwgxyQ9it5Nuv1YtVir0LqTRvROt9JthZVa_PvT6Y1Y22sBoVzBWBB4uRdw9ueIfhCt9gqNkR3a0YscOGesTAPIJlA5673D5q4IULHzU9z6KXZm7Z5u_RQ85D3_u8P7rL2BAXixB6RX0jROdkr7ey4DXgLkgTueuI1eb260Q-Hb4FKQBSEve-BUMAE535HvJxLD5K81OuGVxk5hHbLUIGqr_9P0H9NWy10</recordid><startdate>20020501</startdate><enddate>20020501</enddate><creator>Ishiguro, Naoko</creator><creator>Baba, Takeshi</creator><creator>Ishida, Tsuyoshi</creator><creator>Takeuchi, Kengo</creator><creator>Osaki, Mitsuhiko</creator><creator>Araki, Nobuhito</creator><creator>Okada, Eikichi</creator><creator>Takahashi, Satsuki</creator><creator>Saito, Masahiro</creator><creator>Watanabe, Mari</creator><creator>Nakada, Chisato</creator><creator>Tsukamoto, Yoshiyuki</creator><creator>Sato, Kenzo</creator><creator>Ito, Kinji</creator><creator>Fukayama, Masashi</creator><creator>Mori, Shigeo</creator><creator>Ito, Hisao</creator><creator>Moriyama, Masatsugu</creator><general>Elsevier Inc</general><general>ASIP</general><general>American Society for Investigative Pathology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20020501</creationdate><title>Carp, a Cardiac Ankyrin-Repeated Protein, and Its New Homologue, Arpp, Are Differentially Expressed in Heart, Skeletal Muscle, and Rhabdomyosarcomas</title><author>Ishiguro, Naoko ; Baba, Takeshi ; Ishida, Tsuyoshi ; Takeuchi, Kengo ; Osaki, Mitsuhiko ; Araki, Nobuhito ; Okada, Eikichi ; Takahashi, Satsuki ; Saito, Masahiro ; Watanabe, Mari ; Nakada, Chisato ; Tsukamoto, Yoshiyuki ; Sato, Kenzo ; Ito, Kinji ; Fukayama, Masashi ; Mori, Shigeo ; Ito, Hisao ; Moriyama, Masatsugu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c591t-508c061c06cb850dbe4bc79c4318d70e5cb4e3b788cf7c8cae69a672401617e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Actins - analysis</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies - immunology</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Child</topic><topic>Desmin - analysis</topic><topic>Diseases of the osteoarticular system</topic><topic>Female</topic><topic>Fetal Heart - chemistry</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Leiomyosarcoma - metabolism</topic><topic>Leiomyosarcoma - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Muscle Fibers, Slow-Twitch - chemistry</topic><topic>Muscle Proteins</topic><topic>Muscle, Skeletal - chemistry</topic><topic>Muscle, Skeletal - cytology</topic><topic>Muscle, Smooth - chemistry</topic><topic>Myocardium - cytology</topic><topic>Myocardium - metabolism</topic><topic>Myoglobin - analysis</topic><topic>Nuclear Proteins - biosynthesis</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - immunology</topic><topic>Regular</topic><topic>Repressor Proteins - biosynthesis</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - immunology</topic><topic>Rhabdomyosarcoma - metabolism</topic><topic>Rhabdomyosarcoma - pathology</topic><topic>Sequence Homology, Amino Acid</topic><topic>Tumors of striated muscle and skeleton</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ishiguro, Naoko</creatorcontrib><creatorcontrib>Baba, Takeshi</creatorcontrib><creatorcontrib>Ishida, Tsuyoshi</creatorcontrib><creatorcontrib>Takeuchi, Kengo</creatorcontrib><creatorcontrib>Osaki, Mitsuhiko</creatorcontrib><creatorcontrib>Araki, Nobuhito</creatorcontrib><creatorcontrib>Okada, Eikichi</creatorcontrib><creatorcontrib>Takahashi, Satsuki</creatorcontrib><creatorcontrib>Saito, Masahiro</creatorcontrib><creatorcontrib>Watanabe, Mari</creatorcontrib><creatorcontrib>Nakada, Chisato</creatorcontrib><creatorcontrib>Tsukamoto, Yoshiyuki</creatorcontrib><creatorcontrib>Sato, Kenzo</creatorcontrib><creatorcontrib>Ito, Kinji</creatorcontrib><creatorcontrib>Fukayama, Masashi</creatorcontrib><creatorcontrib>Mori, Shigeo</creatorcontrib><creatorcontrib>Ito, Hisao</creatorcontrib><creatorcontrib>Moriyama, Masatsugu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The American journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ishiguro, Naoko</au><au>Baba, Takeshi</au><au>Ishida, Tsuyoshi</au><au>Takeuchi, Kengo</au><au>Osaki, Mitsuhiko</au><au>Araki, Nobuhito</au><au>Okada, Eikichi</au><au>Takahashi, Satsuki</au><au>Saito, Masahiro</au><au>Watanabe, Mari</au><au>Nakada, Chisato</au><au>Tsukamoto, Yoshiyuki</au><au>Sato, Kenzo</au><au>Ito, Kinji</au><au>Fukayama, Masashi</au><au>Mori, Shigeo</au><au>Ito, Hisao</au><au>Moriyama, Masatsugu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carp, a Cardiac Ankyrin-Repeated Protein, and Its New Homologue, Arpp, Are Differentially Expressed in Heart, Skeletal Muscle, and Rhabdomyosarcomas</atitle><jtitle>The American journal of pathology</jtitle><addtitle>Am J Pathol</addtitle><date>2002-05-01</date><risdate>2002</risdate><volume>160</volume><issue>5</issue><spage>1767</spage><epage>1778</epage><pages>1767-1778</pages><issn>0002-9440</issn><eissn>1525-2191</eissn><coden>AJPAA4</coden><abstract>Arpp, a protein containing an ankyrin repeat domain, PEST sequence, and proline-rich region, is a novel ankyrin-repeated protein highly homologous to Carp, which is proposed to be the putative genetic marker for cardiac hypertrophy. In this study, we comparatively analyzed expression of Arpp and Carp protein in skeletal and cardiac muscles and rhabdomyosarcomas (RMSs). In adult skeletal muscle, Arpp was preferentially expressed in the nucleus and cytoplasm of type I fibers, whereas Carp was barely detectable in skeletal muscle. On the other hand, in adult cardiac muscle, interestingly, Arpp was expressed in ventricles mostly, whereas Carp was expressed throughout the atrium and ventricle. Furthermore, although Carp was identified in fetal heart at 11 developmental weeks, Arpp was very low or undetectable in these fetal hearts. These results suggest that Arpp and Carp are differentially expressed and function in both skeletal and cardiac muscle of fetus and adult. We found that Arpp expression was induced during the differentiation of C2C12 cells
in vitro
, suggesting that Arpp-expression may be associated with the differentiation stage during myogenesis. Both Arpp and Carp were found to be expressed in all of the RMS cases studied. Because the expression patterns of Arpp in RMS were different from those of muscle actin or desmin, Arpp may be detectable in RMS cases that do not express other existing RMS markers.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>12000728</pmid><doi>10.1016/S0002-9440(10)61123-6</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The American journal of pathology, 2002-05, Vol.160 (5), p.1767-1778 |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Actins - analysis Adolescent Adult Aged Amino Acid Sequence Animals Antibodies - immunology Antibody Specificity Biological and medical sciences Cell Differentiation Cell Line Child Desmin - analysis Diseases of the osteoarticular system Female Fetal Heart - chemistry HeLa Cells Humans Immunohistochemistry Leiomyosarcoma - metabolism Leiomyosarcoma - pathology Male Medical sciences Middle Aged Molecular Sequence Data Muscle Fibers, Slow-Twitch - chemistry Muscle Proteins Muscle, Skeletal - chemistry Muscle, Skeletal - cytology Muscle, Smooth - chemistry Myocardium - cytology Myocardium - metabolism Myoglobin - analysis Nuclear Proteins - biosynthesis Nuclear Proteins - genetics Nuclear Proteins - immunology Regular Repressor Proteins - biosynthesis Repressor Proteins - genetics Repressor Proteins - immunology Rhabdomyosarcoma - metabolism Rhabdomyosarcoma - pathology Sequence Homology, Amino Acid Tumors of striated muscle and skeleton |
| title | Carp, a Cardiac Ankyrin-Repeated Protein, and Its New Homologue, Arpp, Are Differentially Expressed in Heart, Skeletal Muscle, and Rhabdomyosarcomas |
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