APOBEC-1 and AID are nucleo-cytoplasmic trafficking proteins but APOBEC3G cannot traffic
Human APOBEC3G (hA3G) is a member of the APOBEC-1 related protein (ARP) family of cytidine deaminases. hA3G functions as a natural defense against endogenous retrotransposons and a multitude of retroviruses, most notably human immunodeficiency virus type 1 (HIV-1). Nothing is known about the cellula...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2006-11, Vol.350 (1), p.214-219 |
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| creator | Bennett, Ryan P. Diner, Elie Sowden, Mark P. Lees, Joshua A. Wedekind, Joseph E. Smith, Harold C. |
| description | Human APOBEC3G (hA3G) is a member of the APOBEC-1 related protein (ARP) family of cytidine deaminases. hA3G functions as a natural defense against endogenous retrotransposons and a multitude of retroviruses, most notably human immunodeficiency virus type 1 (HIV-1). Nothing is known about the cellular function of hA3G, however, upon HIV-1 infection hA3G functions as an antiviral factor by mutating viral single-stranded DNA during reverse transcription. Whereas homologous deaminases such as APOBEC-1 and AID act on RNA and DNA, respectively, in the cell nucleus, hA3G mutagenic activity appears to be restricted to the cytoplasm. We demonstrate that hA3G is not a nucleo-cytoplasmic shuttling protein like APOBEC-1 and AID, but is strongly retained in the cytoplasm through a mechanism that involves both the N and C-terminal regions of the protein. |
| doi_str_mv | 10.1016/j.bbrc.2006.09.032 |
| format | Article |
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Nothing is known about the cellular function of hA3G, however, upon HIV-1 infection hA3G functions as an antiviral factor by mutating viral single-stranded DNA during reverse transcription. Whereas homologous deaminases such as APOBEC-1 and AID act on RNA and DNA, respectively, in the cell nucleus, hA3G mutagenic activity appears to be restricted to the cytoplasm. 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Nothing is known about the cellular function of hA3G, however, upon HIV-1 infection hA3G functions as an antiviral factor by mutating viral single-stranded DNA during reverse transcription. Whereas homologous deaminases such as APOBEC-1 and AID act on RNA and DNA, respectively, in the cell nucleus, hA3G mutagenic activity appears to be restricted to the cytoplasm. 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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | AID Amino Acid Sequence APOBEC-1 APOBEC-1 Deaminase APOBEC3G Cell Nucleus - metabolism Cytidine Deaminase - chemistry Cytidine Deaminase - genetics Cytidine Deaminase - metabolism Cytoplasm - metabolism HeLa Cells Human immunodeficiency virus 1 Humans Leucine - genetics Leucine - metabolism Molecular Sequence Data NES NLS Protein Transport Retrovirus Sequence Alignment Sequence Homology, Amino Acid Trafficking |
| title | APOBEC-1 and AID are nucleo-cytoplasmic trafficking proteins but APOBEC3G cannot traffic |
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