Ultrastructural evidence for co-localization of corticotropin-releasing factor receptor and μ-opioid receptor in the rat nucleus locus coeruleus
Previous studies have shown that corticotropin-releasing factor (CRF), an integral mediator of the stress response, and opioids regulate the activity of the locus-coeruleus-norepinephrine (LC-NE) system during stress in a reciprocal manner. Furthermore, repeated opiate exposure sensitizes noradrener...
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| Veröffentlicht in: | Neuroscience letters 2007-02, Vol.413 (3), p.216-221 |
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| creator | Reyes, Beverly A.S. Glaser, Julia D. Van Bockstaele, Elisabeth J. |
| description | Previous studies have shown that corticotropin-releasing factor (CRF), an integral mediator of the stress response, and opioids regulate the activity of the locus-coeruleus-norepinephrine (LC-NE) system during stress in a reciprocal manner. Furthermore, repeated opiate exposure sensitizes noradrenergic neurons to CRF. Previous studies have shown that μ-opioid receptors (μORs) are prominently distributed within somatodendritic processes of catecholaminergic neurons in the LC and axon terminals containing opioid peptides and CRF converge within the LC. To further examine cellular sites for interactions between CRF receptor type 1 (CRFr) and μOR, immunofluorescence and electron microscopic analysis of the rat LC was conducted. Triple immunofluorescence showed prominent co-localization of the CRFr and μOR in noradrenergic somata in the LC. Ultrastructural analysis confirmed dual localization of CRFr and μOR in common dendritic processes in the LC. Semi-quantitative analysis showed that of the dendrites exhibiting CRFr immunolabeling, 57% expressed μOR immunoreactivity. These data provide ultrastructural evidence that CRFr and μOR are co-localized in LC neurons, a cellular substrate that may underlie opiate-induced sensitization of brain noradrenergic neurons to CRF. |
| doi_str_mv | 10.1016/j.neulet.2006.11.069 |
| format | Article |
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These data provide ultrastructural evidence that CRFr and μOR are co-localized in LC neurons, a cellular substrate that may underlie opiate-induced sensitization of brain noradrenergic neurons to CRF.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/j.neulet.2006.11.069</identifier><identifier>PMID: 17194545</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Biological and medical sciences ; Corticotropin-releasing factor receptor 1 ; Electron microscopy ; Fluorescent Antibody Technique - methods ; Fundamental and applied biological sciences. Psychology ; Immunocytochemistry ; Locus Coeruleus - metabolism ; Locus Coeruleus - ultrastructure ; Male ; Microscopy, Immunoelectron - methods ; Models, Biological ; Rats ; Rats, Sprague-Dawley ; Receptors, Corticotropin-Releasing Hormone - metabolism ; Receptors, Opioid, mu - metabolism ; Tyrosine 3-Monooxygenase - metabolism ; Vertebrates: nervous system and sense organs ; μ-Opioid receptor</subject><ispartof>Neuroscience letters, 2007-02, Vol.413 (3), p.216-221</ispartof><rights>2006 Elsevier Ireland Ltd</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-c2b3642d8ab711496813d5229ff7d5bd804a903c3c4fc6941d0172d46a12f5d13</citedby><cites>FETCH-LOGICAL-c491t-c2b3642d8ab711496813d5229ff7d5bd804a903c3c4fc6941d0172d46a12f5d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.neulet.2006.11.069$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18547612$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17194545$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reyes, Beverly A.S.</creatorcontrib><creatorcontrib>Glaser, Julia D.</creatorcontrib><creatorcontrib>Van Bockstaele, Elisabeth J.</creatorcontrib><title>Ultrastructural evidence for co-localization of corticotropin-releasing factor receptor and μ-opioid receptor in the rat nucleus locus coeruleus</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>Previous studies have shown that corticotropin-releasing factor (CRF), an integral mediator of the stress response, and opioids regulate the activity of the locus-coeruleus-norepinephrine (LC-NE) system during stress in a reciprocal manner. Furthermore, repeated opiate exposure sensitizes noradrenergic neurons to CRF. Previous studies have shown that μ-opioid receptors (μORs) are prominently distributed within somatodendritic processes of catecholaminergic neurons in the LC and axon terminals containing opioid peptides and CRF converge within the LC. To further examine cellular sites for interactions between CRF receptor type 1 (CRFr) and μOR, immunofluorescence and electron microscopic analysis of the rat LC was conducted. Triple immunofluorescence showed prominent co-localization of the CRFr and μOR in noradrenergic somata in the LC. Ultrastructural analysis confirmed dual localization of CRFr and μOR in common dendritic processes in the LC. Semi-quantitative analysis showed that of the dendrites exhibiting CRFr immunolabeling, 57% expressed μOR immunoreactivity. These data provide ultrastructural evidence that CRFr and μOR are co-localized in LC neurons, a cellular substrate that may underlie opiate-induced sensitization of brain noradrenergic neurons to CRF.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Corticotropin-releasing factor receptor 1</subject><subject>Electron microscopy</subject><subject>Fluorescent Antibody Technique - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunocytochemistry</subject><subject>Locus Coeruleus - metabolism</subject><subject>Locus Coeruleus - ultrastructure</subject><subject>Male</subject><subject>Microscopy, Immunoelectron - methods</subject><subject>Models, Biological</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Corticotropin-Releasing Hormone - metabolism</subject><subject>Receptors, Opioid, mu - metabolism</subject><subject>Tyrosine 3-Monooxygenase - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>μ-Opioid receptor</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUuOEzEQhlsIxISBGyDkDbPr4HK7H94goREvaSQ2zNpy7PKMI8cOtjsS3IL7cIY5E24lIrBh40f5_6vK9TXNS6BroDC82a4Dzh7LmlE6rAHWdBCPmhVMI2tHMbLHzYp2lLed4PSieZbzllLaQ8-fNhcwguA971fNz1tfksolzbrMSXmCB2cwaCQ2JqJj66NW3v1QxcVAoq2hVJyOJcW9C21Cjyq7cEes0qU6EmrcLwcVDHn41VZVdOYcdoGUeyRJFRJm7XHOpFaoq46Y5uX-vHlilc_44rRfNrcf3n-9_tTefPn4-frdTau5gNJqtukGzsykNiMAF8MEnekZE9aOpt-YiXIlaKc7za0eBAdDYWSGDwqY7Q10l83bY979vNmh0RjqILzcJ7dT6buMysl_X4K7l3fxIGHqhOiXBFenBCl-mzEXuXNZo_cqYJyzHKYqqyCqkB-FOsWcE9o_RYDKhaXcyiNLubCUALKyrLZXfzd4Np3gVcHrk0DlCskmFbTLZ93U83EAdv4p1nEeHCaZtVsYG1e5FGmi-38nvwEJ6MUi</recordid><startdate>20070221</startdate><enddate>20070221</enddate><creator>Reyes, Beverly A.S.</creator><creator>Glaser, Julia D.</creator><creator>Van Bockstaele, Elisabeth J.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20070221</creationdate><title>Ultrastructural evidence for co-localization of corticotropin-releasing factor receptor and μ-opioid receptor in the rat nucleus locus coeruleus</title><author>Reyes, Beverly A.S. ; Glaser, Julia D. ; Van Bockstaele, Elisabeth J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-c2b3642d8ab711496813d5229ff7d5bd804a903c3c4fc6941d0172d46a12f5d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Corticotropin-releasing factor receptor 1</topic><topic>Electron microscopy</topic><topic>Fluorescent Antibody Technique - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunocytochemistry</topic><topic>Locus Coeruleus - metabolism</topic><topic>Locus Coeruleus - ultrastructure</topic><topic>Male</topic><topic>Microscopy, Immunoelectron - methods</topic><topic>Models, Biological</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Corticotropin-Releasing Hormone - metabolism</topic><topic>Receptors, Opioid, mu - metabolism</topic><topic>Tyrosine 3-Monooxygenase - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>μ-Opioid receptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reyes, Beverly A.S.</creatorcontrib><creatorcontrib>Glaser, Julia D.</creatorcontrib><creatorcontrib>Van Bockstaele, Elisabeth J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reyes, Beverly A.S.</au><au>Glaser, Julia D.</au><au>Van Bockstaele, Elisabeth J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrastructural evidence for co-localization of corticotropin-releasing factor receptor and μ-opioid receptor in the rat nucleus locus coeruleus</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2007-02-21</date><risdate>2007</risdate><volume>413</volume><issue>3</issue><spage>216</spage><epage>221</epage><pages>216-221</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Previous studies have shown that corticotropin-releasing factor (CRF), an integral mediator of the stress response, and opioids regulate the activity of the locus-coeruleus-norepinephrine (LC-NE) system during stress in a reciprocal manner. Furthermore, repeated opiate exposure sensitizes noradrenergic neurons to CRF. Previous studies have shown that μ-opioid receptors (μORs) are prominently distributed within somatodendritic processes of catecholaminergic neurons in the LC and axon terminals containing opioid peptides and CRF converge within the LC. To further examine cellular sites for interactions between CRF receptor type 1 (CRFr) and μOR, immunofluorescence and electron microscopic analysis of the rat LC was conducted. Triple immunofluorescence showed prominent co-localization of the CRFr and μOR in noradrenergic somata in the LC. Ultrastructural analysis confirmed dual localization of CRFr and μOR in common dendritic processes in the LC. Semi-quantitative analysis showed that of the dendrites exhibiting CRFr immunolabeling, 57% expressed μOR immunoreactivity. These data provide ultrastructural evidence that CRFr and μOR are co-localized in LC neurons, a cellular substrate that may underlie opiate-induced sensitization of brain noradrenergic neurons to CRF.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>17194545</pmid><doi>10.1016/j.neulet.2006.11.069</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Corticotropin-releasing factor receptor 1 Electron microscopy Fluorescent Antibody Technique - methods Fundamental and applied biological sciences. Psychology Immunocytochemistry Locus Coeruleus - metabolism Locus Coeruleus - ultrastructure Male Microscopy, Immunoelectron - methods Models, Biological Rats Rats, Sprague-Dawley Receptors, Corticotropin-Releasing Hormone - metabolism Receptors, Opioid, mu - metabolism Tyrosine 3-Monooxygenase - metabolism Vertebrates: nervous system and sense organs μ-Opioid receptor |
| title | Ultrastructural evidence for co-localization of corticotropin-releasing factor receptor and μ-opioid receptor in the rat nucleus locus coeruleus |
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