Visualization of Trichoderma reesei cellobiohydrolase I and endoglucanase I on aspen cellulose by using monoclonal antibody-colloidal gold conjugates

Monoclonal antibodies (MAbs) specific for cellobiohydrolase I (CBH 1) and endoglucanase I (EG 1) were conjugated to 10- and 15-nm colloidal gold particles, respectively. The binding of CBH I and EG I was visualized by utilizing the MAb-colloidal gold probes. The visualization procedure involved immo...

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Veröffentlicht in:	Applied and Environmental Microbiology 1991-11, Vol.57 (11), p.3163-3170
Hauptverfasser:	Nieves, R.A. (Solar Energy Research Institute, Golden, CO), Ellis, R.P, Todd, R.J, Johnson, T.J.A, Grohmann, K, Himmel, M.E
Format:	Artikel
Sprache:	eng
Schlagworte:	ANTIBODIES ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES BASIC BIOLOGICAL SCIENCES BIODEGRADATION CARBOHYDRATES cellobiohydrolase I CELLULASE CELLULOSE CELULOSA CHEMICAL REACTIONS DECOMPOSITION ELECTRON MICROSCOPY endoglucanase I ENZIMAS ENZYME ENZYME ACTIVITY ENZYMES FUNGI GLYCOSYL HYDROLASES HIDROLASAS HYDROLASE HYDROLASES MICROSCOPY MONOCLONAL ANTIBODIES O-GLYCOSYL HYDROLASES ORGANIC COMPOUNDS Physiology and Biotechnology PLANTS POLYSACCHARIDES PROTEINS SACCHARIDES 550200 > Biochemistry TRANSMISSION ELECTRON MICROSCOPY TRICHODERMA
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