Identification of Phytophthora citrophthora with cloned DNA probes
Two different DNA fragments, one of 2.9 kilobases and the other of 5.1 kilobases, were cloned from Phytophthora citrophthora and showed no homology with DNA from plants and other related fungi. These DNA probes hybridized with DNA from 12 different P. citrophthora isolates obtained from a variety of...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1990-03, Vol.56 (3), p.669-674 |
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| container_title | Applied and Environmental Microbiology |
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| creator | Goodwin, P.H. (University of Guelph, Guelph, Ontario, Canada) Kirkpatrick, B.C Duniway, J.M |
| description | Two different DNA fragments, one of 2.9 kilobases and the other of 5.1 kilobases, were cloned from Phytophthora citrophthora and showed no homology with DNA from plants and other related fungi. These DNA probes hybridized with DNA from 12 different P. citrophthora isolates obtained from a variety of hosts but did not hybridize with DNA from 6 P. citrophthora isolates obtained from cacao. Southern blot analysis revealed that the probes contained repetitive DNA, and restriction fragment length polymorphisms were identified among several P. citrophthora isolates. Of the isolates tested, two major groups were observed whose genetic similarity correlated with geographical distribution. One of the DNA probes was used to detect P. citrophthora growing from infected citrus roots incubated on semiselective medium. P. citrophthora was not detected by a hybridization assay of total DNA extracted directly from infected roots |
| doi_str_mv | 10.1128/AEM.56.3.669-674.1990 |
| format | Article |
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(University of Guelph, Guelph, Ontario, Canada) ; Kirkpatrick, B.C ; Duniway, J.M</creator><creatorcontrib>Goodwin, P.H. (University of Guelph, Guelph, Ontario, Canada) ; Kirkpatrick, B.C ; Duniway, J.M</creatorcontrib><description>Two different DNA fragments, one of 2.9 kilobases and the other of 5.1 kilobases, were cloned from Phytophthora citrophthora and showed no homology with DNA from plants and other related fungi. These DNA probes hybridized with DNA from 12 different P. citrophthora isolates obtained from a variety of hosts but did not hybridize with DNA from 6 P. citrophthora isolates obtained from cacao. Southern blot analysis revealed that the probes contained repetitive DNA, and restriction fragment length polymorphisms were identified among several P. citrophthora isolates. Of the isolates tested, two major groups were observed whose genetic similarity correlated with geographical distribution. One of the DNA probes was used to detect P. citrophthora growing from infected citrus roots incubated on semiselective medium. P. citrophthora was not detected by a hybridization assay of total DNA extracted directly from infected roots</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.56.3.669-674.1990</identifier><identifier>PMID: 16348140</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>ADN ; Biological and medical sciences ; CITRUS ; CLONE ; CLONES ; DNA ; Fundamental and applied biological sciences. 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(University of Guelph, Guelph, Ontario, Canada)</creatorcontrib><creatorcontrib>Kirkpatrick, B.C</creatorcontrib><creatorcontrib>Duniway, J.M</creatorcontrib><title>Identification of Phytophthora citrophthora with cloned DNA probes</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Two different DNA fragments, one of 2.9 kilobases and the other of 5.1 kilobases, were cloned from Phytophthora citrophthora and showed no homology with DNA from plants and other related fungi. These DNA probes hybridized with DNA from 12 different P. citrophthora isolates obtained from a variety of hosts but did not hybridize with DNA from 6 P. citrophthora isolates obtained from cacao. Southern blot analysis revealed that the probes contained repetitive DNA, and restriction fragment length polymorphisms were identified among several P. citrophthora isolates. Of the isolates tested, two major groups were observed whose genetic similarity correlated with geographical distribution. One of the DNA probes was used to detect P. citrophthora growing from infected citrus roots incubated on semiselective medium. P. citrophthora was not detected by a hybridization assay of total DNA extracted directly from infected roots</description><subject>ADN</subject><subject>Biological and medical sciences</subject><subject>CITRUS</subject><subject>CLONE</subject><subject>CLONES</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>IDENTIFICACION</subject><subject>IDENTIFICATION</subject><subject>Microbiology</subject><subject>Microorganism-Plant Interactions</subject><subject>Mycological methods and techniques used in mycology</subject><subject>Mycology</subject><subject>PHYTOPHTHORA</subject><subject>probes</subject><subject>restriction fragment length polymorphism</subject><subject>VARIACION GENETICA</subject><subject>VARIATION GENETIQUE</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNp90ctu1DAUBmALgei08AJIoGyAVYbj-L5gMbQFKpWLBF1bZxxnYpTEg52h6tvjakYt3bCyLH-_fayfkJcUlpQ2-t3q_MtSyCVbSmlqqfiSGgOPyIKC0bVgTD4mCwBj6qbhcESOc_4FABykfkqOqGRcUw4L8uGi9dMcuuBwDnGqYld972_muO3nPiasXJjT3eY6zH3lhjj5tjr7uqq2Ka59fkaedDhk__ywnpCrj-c_Tz_Xl98-XZyuLmsnAea6VS0Dh0p76YV03OlGrZtGcCGoYVyhF6oDwbzz1HWNVrzxiKrt1k4xdIadkPf7e7e79ehbV-ZOONhtCiOmGxsx2IcnU-jtJv6xVDMOrOTfHvIp_t75PNsxZOeHAScfd9kqxjhXWogi3_xXUiGEolwVKPbQpZhz8t3dNBTsbU0W_WiFtMyWmmypyd7WVHKv_v3KferQSwGvDwCzw6FLOLmQ753hUkgji6v2rg-b_jokbzGPDx4t5MWedBgtblK55uqHgUaDVOwvp1ev-Q</recordid><startdate>19900301</startdate><enddate>19900301</enddate><creator>Goodwin, P.H. 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(University of Guelph, Guelph, Ontario, Canada)</au><au>Kirkpatrick, B.C</au><au>Duniway, J.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Phytophthora citrophthora with cloned DNA probes</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1990-03-01</date><risdate>1990</risdate><volume>56</volume><issue>3</issue><spage>669</spage><epage>674</epage><pages>669-674</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Two different DNA fragments, one of 2.9 kilobases and the other of 5.1 kilobases, were cloned from Phytophthora citrophthora and showed no homology with DNA from plants and other related fungi. These DNA probes hybridized with DNA from 12 different P. citrophthora isolates obtained from a variety of hosts but did not hybridize with DNA from 6 P. citrophthora isolates obtained from cacao. Southern blot analysis revealed that the probes contained repetitive DNA, and restriction fragment length polymorphisms were identified among several P. citrophthora isolates. Of the isolates tested, two major groups were observed whose genetic similarity correlated with geographical distribution. One of the DNA probes was used to detect P. citrophthora growing from infected citrus roots incubated on semiselective medium. P. citrophthora was not detected by a hybridization assay of total DNA extracted directly from infected roots</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16348140</pmid><doi>10.1128/AEM.56.3.669-674.1990</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0099-2240 |
| ispartof | Applied and Environmental Microbiology, 1990-03, Vol.56 (3), p.669-674 |
| issn | 0099-2240 1098-5336 |
| language | eng |
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| source | American Society for Microbiology; PubMed Central; Alma/SFX Local Collection |
| subjects | ADN Biological and medical sciences CITRUS CLONE CLONES DNA Fundamental and applied biological sciences. Psychology IDENTIFICACION IDENTIFICATION Microbiology Microorganism-Plant Interactions Mycological methods and techniques used in mycology Mycology PHYTOPHTHORA probes restriction fragment length polymorphism VARIACION GENETICA VARIATION GENETIQUE |
| title | Identification of Phytophthora citrophthora with cloned DNA probes |
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