Diagnostic Real-Time PCR Assays for the Detection of Emetic Bacillus cereus Strains in Foods and Recent Food-Borne Outbreaks

Cereulide-producing Bacillus cereus can cause an emetic type of food-borne disease that mimics the symptoms provoked by Staphylococcus aureus. Based on the recently discovered genetic background for cereulide formation, a novel 5' nuclease (TaqMan) real-time PCR assay was developed to provide a...

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Veröffentlicht in:	Applied and Environmental Microbiology 2007-03, Vol.73 (6), p.1892-1898
Hauptverfasser:	Fricker, Martina, Messelhäusser, Ute, Busch, Ulrich, Scherer, Siegfried, Ehling-Schulz, Monika
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus cereus Bacillus cereus - genetics Bacillus cereus - isolation & purification Bacteria Bacteriology Benzothiazoles Biological and medical sciences Brassica Deoxyribonucleic acid Diagnosis, Differential Diamines Disease Outbreaks DNA DNA Primers - genetics Food contamination & poisoning Food Microbiology Foodborne Diseases - diagnosis Foodborne Diseases - epidemiology Foodborne Diseases - microbiology Fundamental and applied biological sciences. Psychology Genetics Humans Microbiology Organic Chemicals - metabolism Oryza sativa Pathology Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards Quinolines Real time Reference Standards Sensitivity and Specificity Staining and Labeling - methods Staphylococcus aureus
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