Development of a New, Combined Rapid Method Using Phage and PCR for Detection and Identification of Viable Mycobacterium paratuberculosis Bacteria within 48 Hours
The FASTPlaqueTB assay is an established diagnostic aid for the rapid detection of Mycobacterium tuberculosis from human sputum samples. Using the FASTPlaqueTB assay reagents, viable Mycobacterium avium subsp. paratuberculosis cells were detected as phage plaques in just 24 h. The bacteriophage used...
Gespeichert in:
| Veröffentlicht in: | Applied and Environmental Microbiology 2007-03, Vol.73 (6), p.1851-1857 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1857 |
|---|---|
| container_issue | 6 |
| container_start_page | 1851 |
| container_title | Applied and Environmental Microbiology |
| container_volume | 73 |
| creator | Stanley, Emma C Mole, Richard J Smith, Rebecca J Glenn, Sarah M Barer, Michael R McGowan, Michael Rees, Catherine E.D |
| description | The FASTPlaqueTB assay is an established diagnostic aid for the rapid detection of Mycobacterium tuberculosis from human sputum samples. Using the FASTPlaqueTB assay reagents, viable Mycobacterium avium subsp. paratuberculosis cells were detected as phage plaques in just 24 h. The bacteriophage used does not infect M. avium subsp. paratuberculosis alone, so to add specificity to this assay, a PCR-based identification method was introduced to amplify M. avium subsp. paratuberculosis-specific sequences from the DNA of the mycobacterial cell detected by the phage. To give further diagnostic information, a multiplex PCR method was developed to allow simultaneous amplification of either M. avium subsp. paratuberculosis or M. tuberculosis complex-specific sequences from plaque samples. Combining the plaque PCR technique with the phage-based detection assay allowed the rapid and specific detection of viable M. avium subsp. paratuberculosis in milk samples in just 48 h. |
| doi_str_mv | 10.1128/AEM.01722-06 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1828794</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1234586021</sourcerecordid><originalsourceid>FETCH-LOGICAL-c520t-996ba14817d880f24379f1bc52ea6186dcd382400e425afe5ef19e543a83f56e3</originalsourceid><addsrcrecordid>eNpd0cFu1DAQBuAIgehSuHEGgwSnpthO7NgXpLIttFIXqsJytSbJZOMqiRc76aqvw5NidlcUOFma-fRrxpMkzxk9Zoyrdydni2PKCs5TKh8kM0a1SkWWyYfJjFKtU85zepA8CeGGUppTqR4nB5ELnUk-S36e4i12bt3jMBLXECCfcXNE5q4v7YA1uYa1rckCx9bVZBnssCJXLayQwFCTq_k1aZwnpzhiNVo3bKsXdcyyja1gW4qh3y2UHZLFXeVKqEb0durJGjyMU4m-mjoXbCAfdi0gGzu2diC5Iudu8uFp8qiBLuCz_XuYLD-efZufp5dfPl3MTy7TSnA6plrLEliuWFErRRueZ4VuWBmbCJIpWVd1puJfUMy5gAYFNkyjyDNQWSMkZofJ-13ueip7rKu4hYfOrL3twd8ZB9b82xlsa1bu1jDFVaHzGPB2H-DdjwnDaHobKuw6GNBNwTCtVa65iPD1f_AmLjrE5QynQhcs5zyiox2qvAvBY_NnEkbN78ubeHmzvbyhMvIXf09_j_enjuDNHkCooGs8DJUN905JRnmho3u1c61dtRvr0UDoDWBviszI6ASL5uXONOAMrHzMWX7llGWUFqIohMx-AalEy68</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>205971422</pqid></control><display><type>article</type><title>Development of a New, Combined Rapid Method Using Phage and PCR for Detection and Identification of Viable Mycobacterium paratuberculosis Bacteria within 48 Hours</title><source>American Society for Microbiology</source><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Stanley, Emma C ; Mole, Richard J ; Smith, Rebecca J ; Glenn, Sarah M ; Barer, Michael R ; McGowan, Michael ; Rees, Catherine E.D</creator><creatorcontrib>Stanley, Emma C ; Mole, Richard J ; Smith, Rebecca J ; Glenn, Sarah M ; Barer, Michael R ; McGowan, Michael ; Rees, Catherine E.D</creatorcontrib><description>The FASTPlaqueTB assay is an established diagnostic aid for the rapid detection of Mycobacterium tuberculosis from human sputum samples. Using the FASTPlaqueTB assay reagents, viable Mycobacterium avium subsp. paratuberculosis cells were detected as phage plaques in just 24 h. The bacteriophage used does not infect M. avium subsp. paratuberculosis alone, so to add specificity to this assay, a PCR-based identification method was introduced to amplify M. avium subsp. paratuberculosis-specific sequences from the DNA of the mycobacterial cell detected by the phage. To give further diagnostic information, a multiplex PCR method was developed to allow simultaneous amplification of either M. avium subsp. paratuberculosis or M. tuberculosis complex-specific sequences from plaque samples. Combining the plaque PCR technique with the phage-based detection assay allowed the rapid and specific detection of viable M. avium subsp. paratuberculosis in milk samples in just 48 h.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.01722-06</identifier><identifier>PMID: 17259362</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Bacteria ; Bacteriological Techniques ; Biological and medical sciences ; Cells ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial - genetics ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; Humans ; Methods ; Microbiology ; Milk - microbiology ; Mycobacteriophages - growth & development ; Mycobacterium avium ; Mycobacterium avium subsp. paratuberculosis - genetics ; Mycobacterium avium subsp. paratuberculosis - isolation & purification ; Mycobacterium avium subsp. paratuberculosis - virology ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - isolation & purification ; Mycobacterium tuberculosis - virology ; Polymerase Chain Reaction - methods ; Public Health Microbiology ; Sensitivity and Specificity ; Viral Plaque Assay ; Viruses</subject><ispartof>Applied and Environmental Microbiology, 2007-03, Vol.73 (6), p.1851-1857</ispartof><rights>2007 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Mar 2007</rights><rights>Copyright © 2007, American Society for Microbiology 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520t-996ba14817d880f24379f1bc52ea6186dcd382400e425afe5ef19e543a83f56e3</citedby><cites>FETCH-LOGICAL-c520t-996ba14817d880f24379f1bc52ea6186dcd382400e425afe5ef19e543a83f56e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828794/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828794/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18610279$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17259362$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stanley, Emma C</creatorcontrib><creatorcontrib>Mole, Richard J</creatorcontrib><creatorcontrib>Smith, Rebecca J</creatorcontrib><creatorcontrib>Glenn, Sarah M</creatorcontrib><creatorcontrib>Barer, Michael R</creatorcontrib><creatorcontrib>McGowan, Michael</creatorcontrib><creatorcontrib>Rees, Catherine E.D</creatorcontrib><title>Development of a New, Combined Rapid Method Using Phage and PCR for Detection and Identification of Viable Mycobacterium paratuberculosis Bacteria within 48 Hours</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>The FASTPlaqueTB assay is an established diagnostic aid for the rapid detection of Mycobacterium tuberculosis from human sputum samples. Using the FASTPlaqueTB assay reagents, viable Mycobacterium avium subsp. paratuberculosis cells were detected as phage plaques in just 24 h. The bacteriophage used does not infect M. avium subsp. paratuberculosis alone, so to add specificity to this assay, a PCR-based identification method was introduced to amplify M. avium subsp. paratuberculosis-specific sequences from the DNA of the mycobacterial cell detected by the phage. To give further diagnostic information, a multiplex PCR method was developed to allow simultaneous amplification of either M. avium subsp. paratuberculosis or M. tuberculosis complex-specific sequences from plaque samples. Combining the plaque PCR technique with the phage-based detection assay allowed the rapid and specific detection of viable M. avium subsp. paratuberculosis in milk samples in just 48 h.</description><subject>Animals</subject><subject>Bacteria</subject><subject>Bacteriological Techniques</subject><subject>Biological and medical sciences</subject><subject>Cells</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Methods</subject><subject>Microbiology</subject><subject>Milk - microbiology</subject><subject>Mycobacteriophages - growth & development</subject><subject>Mycobacterium avium</subject><subject>Mycobacterium avium subsp. paratuberculosis - genetics</subject><subject>Mycobacterium avium subsp. paratuberculosis - isolation & purification</subject><subject>Mycobacterium avium subsp. paratuberculosis - virology</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Mycobacterium tuberculosis - virology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Public Health Microbiology</subject><subject>Sensitivity and Specificity</subject><subject>Viral Plaque Assay</subject><subject>Viruses</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0cFu1DAQBuAIgehSuHEGgwSnpthO7NgXpLIttFIXqsJytSbJZOMqiRc76aqvw5NidlcUOFma-fRrxpMkzxk9Zoyrdydni2PKCs5TKh8kM0a1SkWWyYfJjFKtU85zepA8CeGGUppTqR4nB5ELnUk-S36e4i12bt3jMBLXECCfcXNE5q4v7YA1uYa1rckCx9bVZBnssCJXLayQwFCTq_k1aZwnpzhiNVo3bKsXdcyyja1gW4qh3y2UHZLFXeVKqEb0durJGjyMU4m-mjoXbCAfdi0gGzu2diC5Iudu8uFp8qiBLuCz_XuYLD-efZufp5dfPl3MTy7TSnA6plrLEliuWFErRRueZ4VuWBmbCJIpWVd1puJfUMy5gAYFNkyjyDNQWSMkZofJ-13ueip7rKu4hYfOrL3twd8ZB9b82xlsa1bu1jDFVaHzGPB2H-DdjwnDaHobKuw6GNBNwTCtVa65iPD1f_AmLjrE5QynQhcs5zyiox2qvAvBY_NnEkbN78ubeHmzvbyhMvIXf09_j_enjuDNHkCooGs8DJUN905JRnmho3u1c61dtRvr0UDoDWBviszI6ASL5uXONOAMrHzMWX7llGWUFqIohMx-AalEy68</recordid><startdate>20070301</startdate><enddate>20070301</enddate><creator>Stanley, Emma C</creator><creator>Mole, Richard J</creator><creator>Smith, Rebecca J</creator><creator>Glenn, Sarah M</creator><creator>Barer, Michael R</creator><creator>McGowan, Michael</creator><creator>Rees, Catherine E.D</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7QH</scope><scope>5PM</scope></search><sort><creationdate>20070301</creationdate><title>Development of a New, Combined Rapid Method Using Phage and PCR for Detection and Identification of Viable Mycobacterium paratuberculosis Bacteria within 48 Hours</title><author>Stanley, Emma C ; Mole, Richard J ; Smith, Rebecca J ; Glenn, Sarah M ; Barer, Michael R ; McGowan, Michael ; Rees, Catherine E.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c520t-996ba14817d880f24379f1bc52ea6186dcd382400e425afe5ef19e543a83f56e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Bacteria</topic><topic>Bacteriological Techniques</topic><topic>Biological and medical sciences</topic><topic>Cells</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Methods</topic><topic>Microbiology</topic><topic>Milk - microbiology</topic><topic>Mycobacteriophages - growth & development</topic><topic>Mycobacterium avium</topic><topic>Mycobacterium avium subsp. paratuberculosis - genetics</topic><topic>Mycobacterium avium subsp. paratuberculosis - isolation & purification</topic><topic>Mycobacterium avium subsp. paratuberculosis - virology</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Mycobacterium tuberculosis - isolation & purification</topic><topic>Mycobacterium tuberculosis - virology</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Public Health Microbiology</topic><topic>Sensitivity and Specificity</topic><topic>Viral Plaque Assay</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stanley, Emma C</creatorcontrib><creatorcontrib>Mole, Richard J</creatorcontrib><creatorcontrib>Smith, Rebecca J</creatorcontrib><creatorcontrib>Glenn, Sarah M</creatorcontrib><creatorcontrib>Barer, Michael R</creatorcontrib><creatorcontrib>McGowan, Michael</creatorcontrib><creatorcontrib>Rees, Catherine E.D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Aqualine</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stanley, Emma C</au><au>Mole, Richard J</au><au>Smith, Rebecca J</au><au>Glenn, Sarah M</au><au>Barer, Michael R</au><au>McGowan, Michael</au><au>Rees, Catherine E.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a New, Combined Rapid Method Using Phage and PCR for Detection and Identification of Viable Mycobacterium paratuberculosis Bacteria within 48 Hours</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2007-03-01</date><risdate>2007</risdate><volume>73</volume><issue>6</issue><spage>1851</spage><epage>1857</epage><pages>1851-1857</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>The FASTPlaqueTB assay is an established diagnostic aid for the rapid detection of Mycobacterium tuberculosis from human sputum samples. Using the FASTPlaqueTB assay reagents, viable Mycobacterium avium subsp. paratuberculosis cells were detected as phage plaques in just 24 h. The bacteriophage used does not infect M. avium subsp. paratuberculosis alone, so to add specificity to this assay, a PCR-based identification method was introduced to amplify M. avium subsp. paratuberculosis-specific sequences from the DNA of the mycobacterial cell detected by the phage. To give further diagnostic information, a multiplex PCR method was developed to allow simultaneous amplification of either M. avium subsp. paratuberculosis or M. tuberculosis complex-specific sequences from plaque samples. Combining the plaque PCR technique with the phage-based detection assay allowed the rapid and specific detection of viable M. avium subsp. paratuberculosis in milk samples in just 48 h.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>17259362</pmid><doi>10.1128/AEM.01722-06</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0099-2240 |
| ispartof | Applied and Environmental Microbiology, 2007-03, Vol.73 (6), p.1851-1857 |
| issn | 0099-2240 1098-5336 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1828794 |
| source | American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Bacteria Bacteriological Techniques Biological and medical sciences Cells Deoxyribonucleic acid DNA DNA, Bacterial - genetics Food Microbiology Fundamental and applied biological sciences. Psychology Humans Methods Microbiology Milk - microbiology Mycobacteriophages - growth & development Mycobacterium avium Mycobacterium avium subsp. paratuberculosis - genetics Mycobacterium avium subsp. paratuberculosis - isolation & purification Mycobacterium avium subsp. paratuberculosis - virology Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Mycobacterium tuberculosis - virology Polymerase Chain Reaction - methods Public Health Microbiology Sensitivity and Specificity Viral Plaque Assay Viruses |
| title | Development of a New, Combined Rapid Method Using Phage and PCR for Detection and Identification of Viable Mycobacterium paratuberculosis Bacteria within 48 Hours |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T06%3A01%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20a%20New,%20Combined%20Rapid%20Method%20Using%20Phage%20and%20PCR%20for%20Detection%20and%20Identification%20of%20Viable%20Mycobacterium%20paratuberculosis%20Bacteria%20within%2048%20Hours&rft.jtitle=Applied%20and%20Environmental%20Microbiology&rft.au=Stanley,%20Emma%20C&rft.date=2007-03-01&rft.volume=73&rft.issue=6&rft.spage=1851&rft.epage=1857&rft.pages=1851-1857&rft.issn=0099-2240&rft.eissn=1098-5336&rft.coden=AEMIDF&rft_id=info:doi/10.1128/AEM.01722-06&rft_dat=%3Cproquest_pubme%3E1234586021%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=205971422&rft_id=info:pmid/17259362&rfr_iscdi=true |